LOCUS       RC218917                8283 bp    DNA     circular  SYN 
DEFINITION  UBN1 (Myc-DDK-tagged)-Human ubinuclein 1 (UBN1), transcript variant 1
ACCESSION   NM_016936
VERSION     NM_016936.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4440..4531 
                     /label="Myc-DDK"  
     misc_feature    4561..5146 
                     /label="PolyA signal"  
     misc_feature    4618..4637 
                     /label="Restriction Enzyme"  
     misc_feature    5422..6094 
                     /label="ColE1"  
     misc_feature    6524..7318 
                     /label="Kan/Neo"  
     misc_feature    7353..7655 
                     /label="SV40 ori"  
     misc_feature    7741..8218 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4422..4428 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4422 
                     /label="UBN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGGAGCCC CACAGGGTCC AGTTCACCTC TCTCCCAGGT TCCCTGAATC 
     1081 CTGCGTTTTT GAAGAAGTCC CGGAAGGAGG AGGCTGGGGC AGGAGAACAG CATCAGGACT 
     1141 GTGAGCCGGC TGCAGCAGCT GTTCGGATTA CACTCACCCT CTTTGAACCA GATCACAAAC 
     1201 GCTGCCCAGA GTTCTTCTAC CCAGAGCTGG TGAAGAATAT CCGAGGGAAG GTAAAAGGCC 
     1261 TTCAGCCTGG AGATAAGAAG AAAGATCTGT CAGATCCTTT CAATGACGAA GAAAAGGAAA 
     1321 GGCATAAAGT AGAGGCCCTT GCCCGAAAAT TTGAAGAAAA ATACGGTGGA AAGAAACGTA 
     1381 GAAAAGACCG AATACAGGAC TTGATCGATA TGGGGTATGG TTATGATGAA TCCGACTCCT 
     1441 TCATCGATAA CTCTGAGGCG TATGATGAGC TTGTTCCTGC TTCTTTGACT ACGAAGTATG 
     1501 GAGGATTTTA CATTAACTCG GGAACCCTGC AGTTTAGACA AGCATCAGAG TCTGAAGATG 
     1561 ACTTCATTAA AGAAAAGAAG AAAAAATCTC CAAAGAAGCG GAAGTTGAAG GAAGGTGGTG 
     1621 AGAAGATAAA GAAGAAGAAA AAAGATGACA CTTATGACAA GGAGAAGAAA TCGAAAAAGT 
     1681 CCAAGTTTTC CAAAGCCGGC TTCACAGCCC TCAATGCCAG TAAGGAGAAG AAGAAGAAGA 
     1741 AATATTCTGG GGCTTTAAGC GTTAAAGAGA TGCTAAAGAA ATTTCAGAAA GAGAAAGAGG 
     1801 CTCAGAAAAA AAGGGAGGAG GAGCATAAGC CTGTTGCGGT CCCATCAGCG GAAGCTCAGG 
     1861 GCCTGCGGGA ACTGGAGGGT GCCTCTGACC CCTTGCTCTC ACTCTTTGGC TCTACTTCTG 
     1921 ACAACGACTT GCTCCAGGCG GCCACTGCCA TGGACTCGCT GACGGATTTG GACTTGGAGC 
     1981 ATCTGCTCAG TGAGTCTCCA GAAGGAAGTC CCTTCCGAGA TATGGATGAT GGAAGTGATT 
     2041 CCCTTGGGGT GGGATTGGAC CAGGAATTCA GGCAGCCCTC TTCTCTCCCC GAAGGCCTGC 
     2101 CAGCACCCCT GGAGAAGCGC GTTAAGGAGC TGGCTCAGGC TGCCAGAGCT GCTGAGGGGG 
     2161 AGAGCAGACA GAAGTTCTTC ACCCAGGATA TTAATGGAAT CCTATTAGAC ATAGAGGCGC 
     2221 AGACTCGGGA GCTGAGCAGT CAGGTCCGCT CTGGGGTGTA TGCCTATCTT GCGTCATTCC 
     2281 TGCCCTGCAG CAAGGATGCC CTGCTCAAGC GTGCTCGGAA ACTTCACCTC TATGAACAGG 
     2341 GGGGCCGTCT GAAGGAGCCT CTCCAGAAGC TCAAGGAAGC CATTGGCAGG GCGATGCCAG 
     2401 AGCAGATGGC CAAGTACCAG GACGAATGCC AGGCACACAC GCAGGCAAAG GTTGCCAAGA 
     2461 TGCTGGAAGA GGAGAAAGAC AAGGAGCAGA GGGACCGGAT TTGTTCGGAT GAGGAAGAAG 
     2521 ATGAAGAAAA AGGGGGCAGG AGGATAATGG GACCTCGGAA GAAGTTCCAA TGGAATGATG 
     2581 AGATCAGGGA GCTACTGTGC CAGGTGGTGA AGATCAAACT GGAGAGCCAG GACCTGGAGA 
     2641 GGAACAACAA AGCCCAGGCT TGGGAGGACT GTGTGAAGGG CTTTCTGGAT GCGGAAGTCA 
     2701 AGCCCCTCTG GCCCAAAGGC TGGATGCAGG CCAGAACTCT GTTTAAGGAG AGCAGACGAG 
     2761 GCCATGGGCA CCTGACTTCA ATCCTGGCCA AGAAGAAAGT TATGGCCCCT TCTAAAATCA 
     2821 AGGTGAAGGA ATCGTCTACG AAGCCTGATA AAAAGGTTTC TGTCCCATCA GGACAGATTG 
     2881 GTGGCCCCAT TGCTTTGCCC TCAGATCACC AAACAGGAGG CCTGAGTATT GGGGCCTCGA 
     2941 GCAGGGAGCT CCCATCCCAG GCATCGGGCG GCCTTGCTAA CCCTCCTCCT GTCAACCTGG 
     3001 AGGACTCATT GGATGAAGAC TTGATCCGCA ATCCAGCCTC CTCGGTGGAA GCCGTGTCCA 
     3061 AGGAATTGGC TGCATTGAAT AGCAGAGCAG CTGGGAACTC TGAATTCACA CTGCCTGCAC 
     3121 CCTCAAAAGC ACCTGCAGAA AAAGTTGGAG GCGTTTTATG TACAGAAGAA AAAAGGAACT 
     3181 TTGCGAAGCC TAGTCCTTCT GCTCCACCAC CAGCTAGCTC TCTGCAGTCA CCCCTCAATT 
     3241 TTCTGGCAGA ACAGGCTCTG GCACTGGGGC AGTCCTCTCA GGAGAAAAAA CCAGAGAGTT 
     3301 CTGGCTACAA AGAGCTGTCC TGCCAGGCTC CCCTCAATAA GGGCCTGCCA GAAGTACATC 
     3361 AGTCCAAAGC TAAGCACCAC AGCTTGCCAC GGACGTCTCA CGGGCCCCAA GTGGCAGTTC 
     3421 CTGTGCCTGG CCCCCAGGTC AAAGTCTTTC ATGCCGGCAC TCAGCAGCAG AAAAACTTCA 
     3481 CGCCCCCATC TCCATTTGCC AATAAGCTCC AAGGCCCAAA GGCTTCTCCC ACACAGTGTC 
     3541 ATCGTTCCCT CCTGCAGTTA GTGAAGACAG CGGCCAAAGG CCAGGGCTTC CATCCCTCTG 
     3601 CACCAGCCAC CTCAGGAGGC CTGTCAGCCT CCAGCAGCAG CTCTCACAAG ACCCCAGCCT 
     3661 CGTCCTCTTC TGCCCTGAGC CATCCAGCAA AGCCACATTC AGTCAGCTCT GCAGGCTCAT 
     3721 CTTACAAGAA TAATCCCTTT GCCAGCTCAA TCTCCAAACA TGGGGTTTCT TCTGGCAGCT 
     3781 CTTCCTCGGG AGGAACACCA GTCCAGAGTT CTGTTTCTGG GAGCCTGGTC CCTGGCATAC 
     3841 AGCCTCCCTC CGTGGGACAG GCCACCAGCC GACCCGTCCC AAGTTCAGCA GGGAAAAAAA 
     3901 TGCCTGTTTC CCAGAAGTTG ACTCTGGTAG CCCCTCCAGG CGGTCCAAAC GGAGATTCCA 
     3961 GTGGTGGGAC CCAGGGAGTG GCAAAGTTGC TGACCTCGCC GTCCCTAAAG CCCTCTGCAG 
     4021 TTAGTAGTGT GACATCGTCT ACCTCCTTGT CAAAAGGAGC GAGTGGGACT GTGCTGCTGG 
     4081 CCGGCTCCTC TTTGATGGCT TCACCCTACA AATCCAGCAG CCCAAAGCTG TCTGGGGCCA 
     4141 TGAGCTCGAA CTCCTTGGGA ATTATAACCC CTGTCCCTAT TCCTGTCCAT GTGCTCTCCT 
     4201 TCAGCGCTGA CTCCTCTGCC AAAGCAGGGG TCTCCAAGGA TGCCATCGTC ACAGGCCCTG 
     4261 CCCCCGGGTC CTTCCACCAT GGCCTTGGCC ACAGTCTTCT GGCTGGCTTG CACTCCAGCC 
     4321 CGCCCCATGC AGCGCCTCTC CCACACGCTG CGGTGCCCAC CCATATCCCG CAGAGTCTGC 
     4381 CAGGTGCTTC TCAGCTTCAC GGGAAAGGGC CTGCTGTACC ACGGAAATTG ACGCGTACGC 
     4441 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4501 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4561 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4621 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4681 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4741 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4801 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4861 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4921 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4981 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5041 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5101 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5161 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5221 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5281 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5341 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5401 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5461 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5521 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5581 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5641 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5701 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5761 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5821 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5881 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5941 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6001 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6061 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6121 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6181 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6241 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6301 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6361 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6421 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6481 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6541 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6601 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6661 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6721 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6781 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6841 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6901 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6961 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7021 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7081 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7141 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7201 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7261 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7321 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7381 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7441 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7501 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7561 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7621 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7681 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7741 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7801 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7861 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7921 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7981 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8041 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8101 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8161 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8221 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8281 TTT
//