LOCUS       RC218885                8748 bp    DNA     circular  SYN 
DEFINITION  KALRN (Myc-DDK-tagged)-Human kalirin, RhoGEF kinase (KALRN), transcript variant 3
ACCESSION   NM_007064
VERSION     NM_007064.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4905..4996 
                     /label="Myc-DDK"  
     misc_feature    5026..5611 
                     /label="PolyA signal"  
     misc_feature    5083..5102 
                     /label="Restriction Enzyme"  
     misc_feature    5887..6559 
                     /label="ColE1"  
     misc_feature    6989..7783 
                     /label="Kan/Neo"  
     misc_feature    7818..8120 
                     /label="SV40 ori"  
     misc_feature    8206..8683 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4887..4893 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4887 
                     /label="KALRN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGGCGGC GACAGGGCTT ACACCCGAGG TCCCTCTTTG GGGTGGCTCT 
     1081 TTGCTAAGTG CTGCTGTTGC TTCCCGTGTA GAGATGCATA CTCTCATTCC TCAAGCGAGA 
     1141 ATGGAGGCAA GTCCGAGTCC GTGGCCAACC TGCAGGCCCA GCCCTCCCTG AACTCCATCC 
     1201 ACAGTTCCCC GGGTCCCAAG CGCTCCACCA ACACTCTTAA GAAGTGGCTG ACGAGTCCTG 
     1261 TGCGTCGGCT TAACAGCGGG AAGGCAGATG GAAACATCAA AAAGCAGAAG AAAGTTCGCG 
     1321 ATGGTCGGAA GAGCTTTGAC CTGGGATCTC CCAAGCCTGG GGATGAAACA ACCCCTCAGG 
     1381 GAGACAGCGC TGATGAGAAG AGCAAGAAAG GTTGGGGTGA AGATGAGCCG GATGAAGAGT 
     1441 CACACACACC CCTCCCACCA CCTATGAAGA TTTTTGACAA CGACCCTACA CAGGATGAAA 
     1501 TGTCCTCCTC TTTGCTAGCA GCCCGGCAGG CTTCCACTGA AGTACCTACT GCTGCAGACC 
     1561 TTGTCAATGC AATAGAAAAG TTGGTCAAAA ACAAGCTGAG TCTAGAAGGA AGCTCATACC 
     1621 GGGGGAGCTT GAAAGACCCT GCAGGCTGCC TGAATGAGGG GATGGCCCCA CCCACACCTC 
     1681 CTAAAAACCC AGAAGAAGAA CAGAAAGCCA AGGCCCTGAG AGGCAGGATG TTTGTCCTGA 
     1741 ATGAGCTGGT ACAGACAGAG AAAGACTATG TCAAGGATCT GGGCATTGTG GTGGAGGGCT 
     1801 TCATGAAGAG AATAGAAGAA AAGGGTGTCC CTGAGGATAT GCGAGGAAAG GACAAAATCG 
     1861 TGTTTGGAAA TATTCATCAG ATTTATGACT GGCATAAGGA TTTTTTCCTG GCGGAACTGG 
     1921 AAAAGTGTAT CCAGGAGCAA GACAGATTGG CACAGCTCTT TATTAAGCAC GAGCGGAAGC 
     1981 TGCACATCTA CGTGTGGTAT TGTCAGAATA AGCCGCGCTC AGAGTACATC GTTGCTGAGT 
     2041 ATGACGCCTA CTTTGAGGAG GTAAAACAGG AGATAAATCA GAGGCTGACA CTGAGTGACT 
     2101 TCCTCATCAA GCCCATTCAG AGAATAACAA AATACCAGTT GCTCCTCAAG GACTTCCTGA 
     2161 GATACAGTGA GAAGGCTGGT TTGGAGTGTT CAGATATTGA GAAAGCAGTG GAGTTAATGT 
     2221 GCCTTGTTCC CAAACGCTGC AATGACATGA TGAATCTAGG ACGTCTGCAG GGCTTTGAGG 
     2281 GCACTCTGAC TGCTCAGGGG AAGCTGCTGC AGCAGGACAC ATTCTATGTG ATCGAGCTGG 
     2341 ATGCAGGCAT GCAGTCCCGG ACCAAAGAGA GGCGCGTGTT CCTCTTCGAG CAGATTGTCA 
     2401 TCTTCAGTGA ACTGCTCAGG AAGGGATCCC TCACCCCTGG CTACATGTTC AAAAGGAGCA 
     2461 TCAAGATGAA TTACTTGGTC CTGGAGGAGA ATGTGGACAA TGATCCCTGC AAGTTTGCAC 
     2521 TCATGAACAG AGAGACTTCT GAGAGGGTTG TTCTGCAAGC CGCCAACGCT GACATCCAGC 
     2581 AGGCCTGGGT GCAGGACATC AATCAAGTCT TAGAAACACA GCGAGACTTT TTGAATGCAC 
     2641 TGCAATCGCC CATTGAGTAT CAACGGAAAG AAAGGAGCAC AGCTGTGATG AGGTCTCAAC 
     2701 CTGCCAGGCT TCCCCAAGCC AGCCCCAGGC CCTACTCCTC TGTTCCTGCG GGCTCAGAGA 
     2761 AGCCCCCAAA GGGCTCCAGC TATAACCCAC CTCTGCCTCC CCTGAAGATA TCTACCTCCA 
     2821 ATGGCAGTCC AGGGTTTGAA TACCACCAGC CTGGGGACAA GTTCGAAGCC AGCAAGCAGA 
     2881 ACGACCTGGG AGGCTGCAAT GGGACCTCGT CCATGGCCGT GATCAAAGAT TACTATGCAC 
     2941 TGAAGGAGAA TGAAATCTGT GTGAGCCAAG GTGAGGTGGT CCAGGTCCTC GCCGTCAACC 
     3001 AGCAGAACAT GTGTCTGGTG TACCAGCCTG CCAGCGACCA TTCCCCCGCC GCCGAGGGCT 
     3061 GGGTCCCAGG CAGCATCCTG GCGCCCCTCA CCAAAGCCAC AGCAGCAGAA AGTAGTGACG 
     3121 GGAGCATCAA GAAGTCATGT TCATGGCATA CTCTACGCAT GAGAAAGCGG GCGGAAGTGG 
     3181 AGAACACGGG TAAAAATGAA GCCACAGGGC CTCGTAAACC CAAGGATATT CTGGGCAACA 
     3241 AAGTCTCTGT TAAAGAGACG AACAGTTCCG AGGAATCAGA GTGTGATGAT CTTGACCCTA 
     3301 ATACTAGCAT GGAGATCTTA AATCCAAATT TCATCCAAGA AGTGGCCCCA GAATTCCTTG 
     3361 TGCCCTTGGT GGATGTGACC TGCTTGCTTG GGGACACAGT GATACTGCAG TGCAAAGTCT 
     3421 GTGGGCGGCC AAAGCCCACC ATCACTTGGA AGGGTCCAGA CCAGAACATC CTTGACACTG 
     3481 ATAACAGCTC AGCCACATAC ACGGTCTCCT CTTGTGATTC TGGAGAAATC ACCCTGAAGA 
     3541 TCTGTAATCT GATGCCCCAA GACAGTGGGA TTTATACCTG CATAGCAACA AATGACCACG 
     3601 GGACCACATC AACGTCTGCA ACAGTCAAAG TGCAAGGTGT TCCAGCAGCC CCTAACCGCC 
     3661 CCATTGCCCA GGAGAGAAGC TGCACCTCCG TGATTCTCCG CTGGCTGCCC CCCTCCAGCA 
     3721 CAGGAAACTG CACTATTTCT GGTTACACTG TGGAGTACAG AGAGGAAGGT TCTCAGATCT 
     3781 GGCAGCAGTC AGTGGCTTCG ACCTTGGACA CTTACCTCGT CATCGAAGAC CTTAGTCCCG 
     3841 GGTGTCCTTA TCAGTTCAGA GTCAGTGCCA GTAACCCCTG GGGAATCAGC CTTCCCAGCG 
     3901 AGCCCTCGGA GTTTGTGCGA CTTCCAGAAT ATGATGCTGC TGCTGATGGT GCCACCATTT 
     3961 CTTGGAAGGA AAATTTTGAC TCAGCTTACA CTGAGCTGAA TGAAATTGGA AGAGGCCGTT 
     4021 TCTCTATAGT AAAGAAATGC ATTCACAAAG CTACCCGCAA AGATGTGGCT GTGAAATTTG 
     4081 TTAGCAAAAA AATGAAGAAG AAAGAACAGG CTGCCCACGA GGCTGCCCTG CTTCAGCACC 
     4141 TACAGCACCC CCAGTACATC ACTCTCCATG ACACCTATGA GTCCCCCACA TCCTACATCC 
     4201 TGATCTTGGA ACTGATGGAT GATGGCCGGC TCTTAGACTA CCTTATGAAT CATGATGAAC 
     4261 TGATGGAGGA AAAAGTAGCT TTCTATATCC GAGACATCAT GGAGGCTCTG CAGTACCTTC 
     4321 ACAACTGCAG GGTTGCACAT TTGGACATAA AGCCTGAAAA CCTGCTCATT GACCTACGGA 
     4381 TTCCAGTGCC TCGAGTGAAG CTCATTGACT TGGAGGATGC TGTCCAGATC TCGGGTCACT 
     4441 TCCACATTCA CCACCTGCTG GGGAACCCTG AGTTTGCTGC CCCAGAAGTC ATTCAAGGCA 
     4501 TCCCCGTCTC CCTGGGGACA GACATCTGGA GCATCGGGGT TCTGACATAT GTCATGCTGA 
     4561 GTGGGGTCTC CCCCTTCTTG GATGAGAGCA AAGAGGAGAC ATGTATCAAC GTATGCAGGG 
     4621 TGGATTTCAG CTTCCCCCAT GAATACTTCT GTGGTGTGAG CAATGCTGCC AGAGATTTCA 
     4681 TCAATGTGAT CTTACAGGAA GATTTTCGGA GGCGGCCCAC AGCAGCCACA TGCTTGCAGC 
     4741 ATCCATGGCT GCAGCCCCAT AATGGCAGCT ACTCTAAGAT CCCCCTGGAC ACCTCCCGCC 
     4801 TAGCATGCTT CATAGAACGT CGCAAGCACC AGAATGATGT GCGGCCTATT CCCAATGTCA 
     4861 AGAGCTACAT TGTCAACCGG GTGAACCAAG GGACGACGCG TACGCGGCCG CTCGAGCAGA 
     4921 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4981 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     5041 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     5101 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     5161 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     5221 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     5281 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5341 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5401 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5461 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5521 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5581 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5641 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5701 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5761 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5821 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5881 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5941 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     6001 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     6061 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     6121 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     6181 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     6241 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6301 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6361 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6421 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6481 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6541 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6601 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6661 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6721 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6781 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6841 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6901 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6961 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     7021 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     7081 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     7141 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     7201 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     7261 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7321 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7381 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7441 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7501 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7561 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7621 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7681 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7741 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7801 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7861 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7921 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7981 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     8101 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     8161 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     8221 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     8281 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8341 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8401 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8461 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8521 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8581 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8641 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8701 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//