LOCUS       RC218845                8967 bp    DNA     circular  SYN 
DEFINITION  BRD4 (Myc-DDK-tagged)-Human bromodomain containing 4 (BRD4), transcript variant long
ACCESSION   NM_058243
VERSION     NM_058243.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5124..5215 
                     /label="Myc-DDK"  
     misc_feature    5245..5830 
                     /label="PolyA signal"  
     misc_feature    5302..5321 
                     /label="Restriction Enzyme"  
     misc_feature    6106..6778 
                     /label="ColE1"  
     misc_feature    7208..8002 
                     /label="Kan/Neo"  
     misc_feature    8037..8339 
                     /label="SV40 ori"  
     misc_feature    8425..8902 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5106..5112 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5106 
                     /label="BRD4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGCGGAG AGCGGCCCTG GGACGAGATT GAGAAATCTG CCAGTAATGG 
     1081 GGGATGGACT AGAAACTTCC CAAATGTCTA CAACACAGGC CCAGGCCCAA CCCCAGCCAG 
     1141 CCAACGCAGC CAGCACCAAC CCCCCGCCCC CAGAGACCTC CAACCCTAAC AAGCCCAAGA 
     1201 GGCAGACCAA CCAACTGCAA TACCTGCTCA GAGTGGTGCT CAAGACACTA TGGAAACACC 
     1261 AGTTTGCATG GCCTTTCCAG CAGCCTGTGG ATGCCGTCAA GCTGAACCTC CCTGATTACT 
     1321 ATAAGATCAT TAAAACGCCT ATGGATATGG GAACAATAAA GAAGCGCTTG GAAAACAACT 
     1381 ATTACTGGAA TGCTCAGGAA TGTATCCAGG ACTTCAACAC TATGTTTACA AATTGTTACA 
     1441 TCTACAACAA GCCTGGAGAT GACATAGTCT TAATGGCAGA AGCTCTGGAA AAGCTCTTCT 
     1501 TGCAAAAAAT AAATGAGCTA CCCACAGAAG AAACCGAGAT CATGATAGTC CAGGCAAAAG 
     1561 GAAGAGGACG TGGGAGGAAA GAAACAGGGA CAGCAAAACC TGGCGTTTCC ACGGTACCAA 
     1621 ACACAACTCA AGCATCGACT CCTCCGCAGA CCCAGACCCC TCAGCCGAAT CCTCCTCCTG 
     1681 TGCAGGCCAC GCCTCACCCC TTCCCTGCCG TCACCCCGGA CCTCATCGTC CAGACCCCTG 
     1741 TCATGACAGT GGTGCCTCCC CAGCCACTGC AGACGCCCCC GCCAGTGCCC CCCCAGCCAC 
     1801 AACCCCCACC CGCTCCAGCT CCCCAGCCCG TACAGAGCCA CCCACCCATC ATCGCGGCCA 
     1861 CCCCACAGCC TGTGAAGACA AAGAAGGGAG TGAAGAGGAA AGCAGACACC ACCACCCCCA 
     1921 CCACCATTGA CCCCATTCAC GAGCCACCCT CGCTGCCCCC GGAGCCCAAG ACCACCAAGC 
     1981 TGGGCCAGCG GCGGGAGAGC AGCCGGCCTG TGAAACCTCC AAAGAAGGAC GTGCCCGACT 
     2041 CTCAGCAGCA CCCAGCACCA GAGAAGAGCA GCAAGGTCTC GGAGCAGCTC AAGTGCTGCA 
     2101 GCGGCATCCT CAAGGAGATG TTTGCCAAGA AGCACGCCGC CTACGCCTGG CCCTTCTACA 
     2161 AGCCTGTGGA CGTGGAGGCA CTGGGCCTAC ACGACTACTG TGACATCATC AAGCACCCCA 
     2221 TGGACATGAG CACAATCAAG TCTAAACTGG AGGCCCGTGA GTACCGTGAT GCTCAGGAGT 
     2281 TTGGTGCTGA CGTCCGATTG ATGTTCTCCA ACTGCTATAA GTACAACCCT CCTGACCATG 
     2341 AGGTGGTGGC CATGGCCCGC AAGCTCCAGG ATGTGTTCGA AATGCGCTTT GCCAAGATGC 
     2401 CGGACGAGCC TGAGGAGCCA GTGGTGGCCG TGTCCTCCCC GGCAGTGCCC CCTCCCACCA 
     2461 AGGTTGTGGC CCCGCCCTCA TCCAGCGACA GCAGCAGCGA TAGCTCCTCG GACAGTGACA 
     2521 GTTCGACTGA TGACTCTGAG GAGGAGCGAG CCCAGCGGCT GGCTGAGCTC CAGGAGCAGC 
     2581 TCAAAGCCGT GCACGAGCAG CTTGCAGCCC TCTCTCAGCC CCAGCAGAAC AAACCAAAGA 
     2641 AAAAGGAGAA AGACAAGAAG GAAAAGAAAA AAGAAAAGCA CAAAAGGAAA GAGGAAGTGG 
     2701 AAGAGAATAA AAAAAGCAAA GCCAAGGAAC CTCCTCCTAA AAAGACGAAG AAAAATAATA 
     2761 GCAGCAACAG CAATGTGAGC AAGAAGGAGC CAGCGCCCAT GAAGAGCAAG CCCCCTCCCA 
     2821 CGTATGAGTC GGAGGAAGAG GACAAGTGCA AGCCTATGTC CTATGAGGAG AAGCGGCAGC 
     2881 TCAGCTTGGA CATCAACAAG CTCCCCGGCG AGAAGCTGGG CCGCGTGGTG CACATCATCC 
     2941 AGTCACGGGA GCCCTCCCTG AAGAATTCCA ACCCCGACGA GATTGAAATC GACTTTGAGA 
     3001 CCCTGAAGCC GTCCACACTG CGTGAGCTGG AGCGCTATGT CACCTCCTGT TTGCGGAAGA 
     3061 AAAGGAAACC TCAAGCTGAG AAAGTTGATG TGATTGCCGG CTCCTCCAAG ATGAAGGGCT 
     3121 TCTCGTCCTC AGAGTCGGAG AGCTCCAGTG AGTCCAGCTC CTCTGACAGC GAAGACTCCG 
     3181 AAACAGAGAT GGCTCCGAAG TCAAAAAAGA AGGGGCACCC CGGGAGGGAG CAGAAGAAGC 
     3241 ACCATCATCA CCACCATCAG CAGATGCAGC AGGCCCCGGC TCCTGTGCCC CAGCAGCCGC 
     3301 CCCCGCCTCC CCAGCAGCCC CCACCGCCTC CACCTCCGCA GCAGCAACAG CAGCCGCCAC 
     3361 CCCCGCCTCC CCCACCCTCC ATGCCGCAGC AGGCAGCCCC GGCGATGAAG TCCTCGCCCC 
     3421 CACCCTTCAT TGCCACCCAG GTGCCCGTCC TGGAGCCCCA GCTCCCAGGC AGCGTCTTTG 
     3481 ACCCCATCGG CCACTTCACC CAGCCCATCC TGCACCTGCC GCAGCCTGAG CTGCCCCCTC 
     3541 ACCTGCCCCA GCCGCCTGAG CACAGCACTC CACCCCATCT CAACCAGCAC GCAGTGGTCT 
     3601 CTCCTCCAGC TTTGCACAAC GCACTACCCC AGCAGCCATC ACGGCCCAGC AACCGAGCCG 
     3661 CTGCCCTGCC TCCCAAGCCC GCCCGGCCCC CAGCCGTGTC ACCAGCCTTG ACCCAAACAC 
     3721 CCCTGCTCCC ACAGCCCCCC ATGGCCCAAC CCCCCCAAGT GCTGCTGGAG GATGAAGAGC 
     3781 CACCTGCCCC ACCCCTCACC TCCATGCAGA TGCAGCTGTA CCTGCAGCAG CTGCAGAAGG 
     3841 TGCAGCCCCC TACGCCGCTA CTCCCTTCCG TGAAGGTGCA GTCCCAGCCC CCACCCCCCC 
     3901 TGCCGCCCCC ACCCCACCCC TCTGTGCAGC AGCAGCTGCA GCAGCAGCCG CCACCACCCC 
     3961 CACCACCCCA GCCCCAGCCT CCACCCCAGC AGCAGCATCA GCCCCCTCCA CGGCCCGTGC 
     4021 ACTTGCAGCC CATGCAGTTT TCCACCCACA TCCAACAGCC CCCGCCACCC CAGGGCCAGC 
     4081 AGCCCCCCCA TCCGCCCCCA GGCCAGCAGC CACCCCCGCC GCAGCCTGCC AAGCCTCAGC 
     4141 AAGTCATCCA GCACCACCAT TCACCCCGGC ACCACAAGTC GGACCCCTAC TCAACCGGTC 
     4201 ACCTCCGCGA AGCCCCCTCC CCGCTTATGA TACATTCCCC CCAGATGTCA CAGTTCCAGA 
     4261 GCCTGACCCA CCAGTCTCCA CCCCAGCAAA ACGTCCAGCC TAAGAAACAG GAGCTGCGTG 
     4321 CTGCCTCCGT GGTCCAGCCC CAGCCCCTCG TGGTGGTGAA GGAGGAGAAG ATCCACTCAC 
     4381 CCATCATCCG CAGCGAGCCC TTCAGCCCCT CGCTGCGGCC GGAGCCCCCC AAGCACCCGG 
     4441 AGAGCATCAA GGCCCCCGTC CACCTGCCCC AGCGGCCGGA AATGAAGCCT GTGGATGTCG 
     4501 GGAGGCCTGT GATCCGGCCC CCAGAGCAGA ACGCACCGCC ACCAGGGGCC CCTGACAAGG 
     4561 ACAAACAGAA ACAGGAGCCG AAGACTCCAG TTGCGCCCAA AAAGGACCTG AAAATCAAGA 
     4621 ACATGGGCTC CTGGGCCAGC CTAGTGCAGA AGCATCCGAC CACCCCCTCC TCCACAGCCA 
     4681 AGTCATCCAG CGACAGCTTC GAGCAGTTCC GCCGCGCCGC TCGGGAGAAA GAGGAGCGTG 
     4741 AGAAGGCCCT GAAGGCTCAG GCCGAGCACG CTGAGAAGGA GAAGGAGCGG CTGCGGCAGG 
     4801 AGCGCATGAG GAGCCGAGAG GACGAGGATG CGCTGGAGCA GGCCCGGCGG GCCCATGAGG 
     4861 AGGCACGTCG GCGCCAGGAG CAGCAGCAGC AGCAGCGCCA GGAGCAACAG CAGCAGCAGC 
     4921 AACAGCAAGC AGCTGCGGTG GCTGCCGCCG CCACCCCACA GGCCCAGAGC TCCCAGCCCC 
     4981 AGTCCATGCT GGACCAGCAG AGGGAGTTGG CCCGGAAGCG GGAGCAGGAG CGAAGACGCC 
     5041 GGGAAGCCAT GGCAGCTACC ATTGACATGA ATTTCCAGAG TGATCTATTG TCAATATTTG 
     5101 AAGAAAATCT TTTCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5161 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5221 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5281 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5341 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5401 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5461 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5521 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5581 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5641 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5701 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5761 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5821 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5881 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5941 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6001 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6061 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6121 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6181 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6241 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6301 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6361 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6421 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6481 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6541 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6601 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6661 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6721 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6781 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6841 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6901 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6961 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7021 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7081 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7141 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7201 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7261 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7321 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7381 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7441 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7501 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7561 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7621 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7681 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7741 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7801 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7861 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7921 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7981 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8041 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8101 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8161 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8221 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8281 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8341 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8401 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8461 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8521 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8581 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8641 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8701 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8761 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8821 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8881 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8941 ATTTAACAAA AATTTAACGC GAATTTT
//