LOCUS       RC218825L2                9597 bp    DNA     circular  SYN 
DEFINITION  PI4KA (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1
ACCESSION   NM_002650
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7888..8068 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8796..9455 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    8116..8735 
                     /label="ColE1"  
     misc_feature    6763..6783 
                     /label="LR50"  
     misc_feature    5903..6628 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6772..7363 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5882..5888 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5882 
                     /label="PI4KA"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCGGGAGATG GCAGGGGCCT GGCACATGAC 
     3361 GGTGGAGCAG AAATTTGGCC TGTTTTCTGC TGAGATAAAG GAAGCAGACC CCCTGGCTGC 
     3421 CTCGGAAGCA AGTCAACCCA AACCCTGTCC CCCCGAAGTG ACCCCCCACT ACATCTGGAT 
     3481 CGACTTCCTG GTGCAGCGGT TTGAGATCGC CAAGTACTGC AGCTCTGACC AAGTGGAGAT 
     3541 CTTCTCCAGC CTGCTGCAGC GCTCCATGTC CCTGAACATC GGCGGGGCCA AGGGGAGCAT 
     3601 GAACCGGCAC GTGGCGGCCA TCGGGCCCCG CTTCAAGCTG CTGACCCTGG GGCTGTCCCT 
     3661 CCTGCATGCC GATGTGGTTC CAAATGCAAC CATCCGCAAT GTGCTTCGCG AGAAGATCTA 
     3721 CTCCACTGCC TTTGACTACT TCAGCTGTCC CCCAAAGTTC CCTACTCAAG GAGAGAAGCG 
     3781 GCTGCGTGAA GACATAAGCA TCATGATTAA ATTTTGGACC GCCATGTTCT CAGATAAGAA 
     3841 GTACCTGACC GCCAGCCAGC TTGTTCCCCC AGATAATCAG GACACCCGGA GCAACCTGGA 
     3901 CATAACTGTC GGCTCTCGGC AACAAGCCAC CCAAGGCTGG ATCAACACAT ACCCCCTGTC 
     3961 CAGCGGCATG TCCACCATCT CCAAGAAATC AGGCATGTCT AAGAAAACCA ACCGGGGCTC 
     4021 CCAGCTGCAC AAATACTACA TGAAGCGCAG GACGCTGCTG CTGTCCCTGC TGGCCACTGA 
     4081 GATCGAGCGT CTCATCACAT GGTACAACCC GCTGTCAGCC CCGGAACTGG AACTAGACCA 
     4141 GGCCGGAGAG AACAGCGTGG CCAACTGGAG ATCTAAGTAC ATCAGCCTGA GTGAGAAGCA 
     4201 GTGGAAGGAC AACGTGAACC TCGCCTGGAG CATCTCTCCC TACCTAGCCG TGCAGCTGCC 
     4261 TGCCAGGTTT AAGAACACAG AAGCCATTGG GAACGAAGTG ACCCGTCTCG TTCGGTTGGA 
     4321 CCCGGGAGCC GTTAGTGATG TGCCTGAAGC AATCAAGTTC CTGGTCACCT GGCACACCAT 
     4381 CGACGCCGAT GCTCCAGAGC TCAGCCATGT GCTGTGCTGG GCGCCCACGG ACCCACCCAC 
     4441 AGGCCTCTCC TACTTCTCCA GCATGTACCC GCCGCACCCT CTCACGGCGC AGTACGGGGT 
     4501 GAAAGTCCTG CGGTCCTTCC CTCCGGACGC CATCCTCTTC TACATCCCCC AGATTGTGCA 
     4561 GGCCCTCAGG TACGACAAGA TGGGCTATGT GCGGGAGTAT ATTCTGTGGG CAGCGTCTAA 
     4621 ATCCCAGCTT CTGGCACACC AGTTCATCTG GAACATGAAG ACTAACATTT ATCTAGATGA 
     4681 AGAGGGCCAC CAGAAAGACC CTGACATCGG CGACCTCCTG GATCAGTTGG TAGAGGAGAT 
     4741 CACAGGCTCC TTGTCCGGCC CAGCGAAGGA CTTTTACCAG CGGGAGTTTG ATTTCTTTAA 
     4801 CAAGATCACC AACGTGTCGG CTATCATCAA GCCCTACCCT AAAGGCGACG AGAGAAAGAA 
     4861 GGCTTGTCTG TCGGCCCTGT CTGAAGTGAA GGTGCAGCCG GGCTGCTACC TGCCCAGCAA 
     4921 CCCTGAGGCC ATTGTGCTGG ACATCGACTA CAAGTCTGGG ACCCCGATGC AGAGTGCTGC 
     4981 AAAAGCCCCA TATCTGGCCA AGTTCAAGGT GAAGCGATGT GGAGTTAGTG AACTTGAAAA 
     5041 AGAAGGTCTG CGGTGCCGCT CAGACTCCGA GGATGAGTGC AGCACGCAGG AGGCCGACGG 
     5101 CCAGAAGATC TCCTGGCAGG CAGCCATCTT CAAGGTGGGA GACGACTGCC GGCAGGACAT 
     5161 GCTGGCCCTG CAGATCATCG ACCTCTTCAA GAACATCTTC CAGCTGGTCG GCCTGGACCT 
     5221 CTTTGTTTTT CCCTACCGCG TGGTGGCCAC TGCCCCTGGG TGCGGGGTGA TCGAGTGCAT 
     5281 CCCCGACTGC ACCTCCCGGG ACCAGCTGGG CCGCCAGACA GACTTCGGCA TGTACGACTA 
     5341 CTTCACACGC CAGTACGGGG ATGAGTCCAC TCTGGCCTTC CAGCAGGCCC GCTACAACTT 
     5401 CATCCGAAGC ATGGCCGCCT ACAGCCTCCT GCTGTTCCTG CTGCAGATCA AGGACAGACA 
     5461 CAACGGCAAC ATTATGCTGG ACAAGAAGGG TCATATCATC CACATCGACT TTGGCTTCAT 
     5521 GTTTGAAAGC TCGCCGGGCG GCAATCTCGG CTGGGAACCC GACATCAAGC TGACGGATGA 
     5581 GATGGTGATG ATCATGGGGG GCAAGATGGA GGCCACACCC TTCAAGTGGT TCATGGAGAT 
     5641 GTGTGTCCGA GGCTACCTGG CTGTGCGGCC CTACATGGAC GCGGTCGTCT CCCTGGTCAC 
     5701 TCTCATGTTG GACACGGGCC TGCCCTGTTT TCGCGGCCAG ACAATCAAGC TCTTGAAGCA 
     5761 CAGGTTTAGC CCCAACATGA CTGAGCGCGA GGCTGCAAAT TTCATCATGA AGGTCATCCA 
     5821 GAGCTGCTTC CTCAGCAACA GGAGCCGGAC CTACGACATG ATCCAGTACT ATCAGAATGA 
     5881 CATCCCCTAC ACGCGTACGC GGCCGCTCGA GATGAGCGGG GGCGAGGAGC TGTTCGCCGG 
     5941 CATCGTGCCC GTGCTGATCG AGCTGGACGG CGACGTGCAC GGCCACAAGT TCAGCGTGCG 
     6001 CGGCGAGGGC GAGGGCGACG CCGACTACGG CAAGCTGGAG ATCAAGTTCA TCTGCACCAC 
     6061 CGGCAAGCTG CCCGTGCCCT GGCCCACCCT GGTGACCACC CTCTGCTACG GCATCCAGTG 
     6121 CTTCGCCCGC TACCCCGAGC ACATGAAGAT GAACGACTTC TTCAAGAGCG CCATGCCCGA 
     6181 GGGCTACATC CAGGAGCGCA CCATCCAGTT CCAGGACGAC GGCAAGTACA AGACCCGCGG 
     6241 CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC GAGCTGAAGG GCAAGGACTT 
     6301 CAAGGAGGAC GGCAACATCC TGGGCCACAA GCTGGAGTAC AGCTTCAACA GCCACAACGT 
     6361 GTACATCCGC CCCGACAAGG CCAACAACGG CCTGGAGGCT AACTTCAAGA CCCGCCACAA 
     6421 CATCGAGGGC GGCGGCGTGC AGCTGGCCGA CCACTACCAG ACCAACGTGC CCCTGGGCGA 
     6481 CGGCCCCGTG CTGATCCCCA TCAACCACTA CCTGAGCACT CAGACCAAGA TCAGCAAGGA 
     6541 CCGCAACGAG GCCCGCGACC ACATGGTGCT CCTGGAGTCC TTCAGCGCCT GCTGCCACAC 
     6601 CCACGGCATG GACGAGCTGT ACAGGTCCGG ACTCAGATAA GTTTAAACGG CCGGCCGCGG 
     6661 TCTGTACAAG TAGGATTCGT CGAGGGACCT AATAACTTCG TATAGCATAC ATTATACGAA 
     6721 GTTATACATG TTTAAGGGTT CCGGTTCCAC TAGGTACAAT TCGATATCAA GCTTATCGAT 
     6781 AATCAACCTC TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT 
     6841 CCTTTTACGC TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT 
     6901 ATGGCTTTCA TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG 
     6961 TGGCCCGTTG TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT 
     7021 GGTTGGGGCA TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT 
     7081 ATTGCCACGG CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG 
     7141 TTGGGCACTG ACAATTCCGT GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC 
     7201 GCCTGTGTTG CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC 
     7261 AATCCAGCGG ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT 
     7321 CGCCTTCGCC CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCA TCGATACCGT 
     7381 CGACCTCGAT CGAGACCTAG AAAAACATGG AGCAATCACA AGTAGCAATA CAGCAGCTAC 
     7441 CAATGCTGAT TGTGCCTGGC TAGAAGCACA AGAGGAGGAG GAGGTGGGTT TTCCAGTCAC 
     7501 ACCTCAGGTA CCTTTAAGAC CAATGACTTA CAAGGCAGCT GTAGATCTTA GCCACTTTTT 
     7561 AAAAGAAAAG GGGGGACTGG AAGGGCTAAT TCACTCCCAA CGAAGACAAG ATATCCTTGA 
     7621 TCTGTGGATC TACCACACAC AAGGCTACTT CCCTGATTGG CAGAACTACA CACCAGGGCC 
     7681 AGGGATCAGA TATCCACTGA CCTTTGGATG GTGCTACAAG CTAGTACCAG TTGAGCAAGA 
     7741 GAAGGTAGAA GAAGCCAATG AAGGAGAGAA CACCCGCTTG TTACACCCTG TGAGCCTGCA 
     7801 TGGGATGGAT GACCCGGAGA GAGAAGTATT AGAGTGGAGG TTTGACAGCC GCCTAGCATT 
     7861 TCATCACATG GCCCGAGAGC TGCATCCGGA CTGTACTGGG TCTCTCTGGT TAGACCAGAT 
     7921 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT 
     7981 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC 
     8041 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGC ATGTGAGCAA AAGGCCAGCA 
     8101 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     8161 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     8221 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     8281 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     8341 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     8401 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     8461 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     8521 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     8581 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     8641 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     8701 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     8761 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGATTACGC CCCGCCCTGC 
     8821 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAA 
     8881 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT 
     8941 TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA 
     9001 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA 
     9061 GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC 
     9121 TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG 
     9181 AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC 
     9241 ATACGGAACT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA 
     9301 AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC 
     9361 TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT 
     9421 TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATACTCTT CCTTTTTCAA 
     9481 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     9541 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGAC
//