LOCUS       RC218825                7443 bp    DNA     circular  SYN 
DEFINITION  PI4KA (Myc-DDK-tagged)-Human phosphatidylinositol 4-kinase, catalytic, alpha (PI4KA), transcript variant 1
ACCESSION   NM_002650
VERSION     NM_002650.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3600..3691 
                     /label="Myc-DDK"  
     misc_feature    3721..4306 
                     /label="PolyA signal"  
     misc_feature    3778..3797 
                     /label="Restriction Enzyme"  
     misc_feature    4582..5254 
                     /label="ColE1"  
     misc_feature    5684..6478 
                     /label="Kan/Neo"  
     misc_feature    6513..6815 
                     /label="SV40 ori"  
     misc_feature    6901..7378 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3582..3588 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3582 
                     /label="PI4KA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGGAGATG GCAGGGGCCT GGCACATGAC GGTGGAGCAG AAATTTGGCC 
     1081 TGTTTTCTGC TGAGATAAAG GAAGCAGACC CCCTGGCTGC CTCGGAAGCA AGTCAACCCA 
     1141 AACCCTGTCC CCCCGAAGTG ACCCCCCACT ACATCTGGAT CGACTTCCTG GTGCAGCGGT 
     1201 TTGAGATCGC CAAGTACTGC AGCTCTGACC AAGTGGAGAT CTTCTCCAGC CTGCTGCAGC 
     1261 GCTCCATGTC CCTGAACATC GGCGGGGCCA AGGGGAGCAT GAACCGGCAC GTGGCGGCCA 
     1321 TCGGGCCCCG CTTCAAGCTG CTGACCCTGG GGCTGTCCCT CCTGCATGCC GATGTGGTTC 
     1381 CAAATGCAAC CATCCGCAAT GTGCTTCGCG AGAAGATCTA CTCCACTGCC TTTGACTACT 
     1441 TCAGCTGTCC CCCAAAGTTC CCTACTCAAG GAGAGAAGCG GCTGCGTGAA GACATAAGCA 
     1501 TCATGATTAA ATTTTGGACC GCCATGTTCT CAGATAAGAA GTACCTGACC GCCAGCCAGC 
     1561 TTGTTCCCCC AGATAATCAG GACACCCGGA GCAACCTGGA CATAACTGTC GGCTCTCGGC 
     1621 AACAAGCCAC CCAAGGCTGG ATCAACACAT ACCCCCTGTC CAGCGGCATG TCCACCATCT 
     1681 CCAAGAAATC AGGCATGTCT AAGAAAACCA ACCGGGGCTC CCAGCTGCAC AAATACTACA 
     1741 TGAAGCGCAG GACGCTGCTG CTGTCCCTGC TGGCCACTGA GATCGAGCGT CTCATCACAT 
     1801 GGTACAACCC GCTGTCAGCC CCGGAACTGG AACTAGACCA GGCCGGAGAG AACAGCGTGG 
     1861 CCAACTGGAG ATCTAAGTAC ATCAGCCTGA GTGAGAAGCA GTGGAAGGAC AACGTGAACC 
     1921 TCGCCTGGAG CATCTCTCCC TACCTAGCCG TGCAGCTGCC TGCCAGGTTT AAGAACACAG 
     1981 AAGCCATTGG GAACGAAGTG ACCCGTCTCG TTCGGTTGGA CCCGGGAGCC GTTAGTGATG 
     2041 TGCCTGAAGC AATCAAGTTC CTGGTCACCT GGCACACCAT CGACGCCGAT GCTCCAGAGC 
     2101 TCAGCCATGT GCTGTGCTGG GCGCCCACGG ACCCACCCAC AGGCCTCTCC TACTTCTCCA 
     2161 GCATGTACCC GCCGCACCCT CTCACGGCGC AGTACGGGGT GAAAGTCCTG CGGTCCTTCC 
     2221 CTCCGGACGC CATCCTCTTC TACATCCCCC AGATTGTGCA GGCCCTCAGG TACGACAAGA 
     2281 TGGGCTATGT GCGGGAGTAT ATTCTGTGGG CAGCGTCTAA ATCCCAGCTT CTGGCACACC 
     2341 AGTTCATCTG GAACATGAAG ACTAACATTT ATCTAGATGA AGAGGGCCAC CAGAAAGACC 
     2401 CTGACATCGG CGACCTCCTG GATCAGTTGG TAGAGGAGAT CACAGGCTCC TTGTCCGGCC 
     2461 CAGCGAAGGA CTTTTACCAG CGGGAGTTTG ATTTCTTTAA CAAGATCACC AACGTGTCGG 
     2521 CTATCATCAA GCCCTACCCT AAAGGCGACG AGAGAAAGAA GGCTTGTCTG TCGGCCCTGT 
     2581 CTGAAGTGAA GGTGCAGCCG GGCTGCTACC TGCCCAGCAA CCCTGAGGCC ATTGTGCTGG 
     2641 ACATCGACTA CAAGTCTGGG ACCCCGATGC AGAGTGCTGC AAAAGCCCCA TATCTGGCCA 
     2701 AGTTCAAGGT GAAGCGATGT GGAGTTAGTG AACTTGAAAA AGAAGGTCTG CGGTGCCGCT 
     2761 CAGACTCCGA GGATGAGTGC AGCACGCAGG AGGCCGACGG CCAGAAGATC TCCTGGCAGG 
     2821 CAGCCATCTT CAAGGTGGGA GACGACTGCC GGCAGGACAT GCTGGCCCTG CAGATCATCG 
     2881 ACCTCTTCAA GAACATCTTC CAGCTGGTCG GCCTGGACCT CTTTGTTTTT CCCTACCGCG 
     2941 TGGTGGCCAC TGCCCCTGGG TGCGGGGTGA TCGAGTGCAT CCCCGACTGC ACCTCCCGGG 
     3001 ACCAGCTGGG CCGCCAGACA GACTTCGGCA TGTACGACTA CTTCACACGC CAGTACGGGG 
     3061 ATGAGTCCAC TCTGGCCTTC CAGCAGGCCC GCTACAACTT CATCCGAAGC ATGGCCGCCT 
     3121 ACAGCCTCCT GCTGTTCCTG CTGCAGATCA AGGACAGACA CAACGGCAAC ATTATGCTGG 
     3181 ACAAGAAGGG TCATATCATC CACATCGACT TTGGCTTCAT GTTTGAAAGC TCGCCGGGCG 
     3241 GCAATCTCGG CTGGGAACCC GACATCAAGC TGACGGATGA GATGGTGATG ATCATGGGGG 
     3301 GCAAGATGGA GGCCACACCC TTCAAGTGGT TCATGGAGAT GTGTGTCCGA GGCTACCTGG 
     3361 CTGTGCGGCC CTACATGGAC GCGGTCGTCT CCCTGGTCAC TCTCATGTTG GACACGGGCC 
     3421 TGCCCTGTTT TCGCGGCCAG ACAATCAAGC TCTTGAAGCA CAGGTTTAGC CCCAACATGA 
     3481 CTGAGCGCGA GGCTGCAAAT TTCATCATGA AGGTCATCCA GAGCTGCTTC CTCAGCAACA 
     3541 GGAGCCGGAC CTACGACATG ATCCAGTACT ATCAGAATGA CATCCCCTAC ACGCGTACGC 
     3601 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     3661 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     3721 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     3781 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     3841 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     3901 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     3961 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4021 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4081 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4141 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4201 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4261 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4321 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4381 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4441 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4501 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4561 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     4621 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     4681 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     4741 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     4801 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     4861 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     4921 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     4981 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5041 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5101 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5161 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5221 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5281 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5341 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5401 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5461 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5521 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     5581 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     5641 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     5701 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     5761 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     5821 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     5881 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     5941 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6001 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6061 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6121 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6181 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6241 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6301 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6361 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6421 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6481 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6541 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     6601 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     6661 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     6721 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     6781 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     6841 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     6901 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     6961 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7021 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7081 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7141 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7201 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7261 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7321 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7381 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7441 TTT
//