LOCUS       RC218681                7848 bp    DNA     circular  SYN 
DEFINITION  KCNH1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 1 (KCNH1), transcript variant 1
ACCESSION   NM_172362
VERSION     NM_172362.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4005..4096 
                     /label="Myc-DDK"  
     misc_feature    4126..4711 
                     /label="PolyA signal"  
     misc_feature    4183..4202 
                     /label="Restriction Enzyme"  
     misc_feature    4987..5659 
                     /label="ColE1"  
     misc_feature    6089..6883 
                     /label="Kan/Neo"  
     misc_feature    6918..7220 
                     /label="SV40 ori"  
     misc_feature    7306..7783 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3987..3993 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3987 
                     /label="KCNH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCATGGCT GGGGGCAGGA GGGGACTAGT GGCCCCTCAA AACACGTTTC 
     1081 TGGAGAATAT TGTTCGGCGG TCCAATGATA CTAATTTTGT GTTGGGGAAT GCTCAGATAG 
     1141 TGGACTGGCC TATTGTGTAC AGCAATGATG GATTTTGCAA GCTGTCTGGC TATCACAGGG 
     1201 CAGAAGTGAT GCAAAAAAGC AGCACCTGCA GTTTTATGTA TGGGGAGCTG ACTGATAAAG 
     1261 ACACGATTGA AAAAGTGCGG CAAACATTTG AGAACTATGA GATGAATTCC TTTGAAATTC 
     1321 TGATGTACAA GAAGAACAGG ACACCTGTGT GGTTCTTTGT GAAAATTGCT CCAATTCGAA 
     1381 ACGAACAGGA TAAAGTGGTT TTATTTCTTT GCACTTTCAG TGACATAACA GCTTTCAAAC 
     1441 AGCCAATTGA GGATGATTCA TGTAAAGGCT GGGGGAAGTT TGCTCGGCTG ACAAGAGCAC 
     1501 TGACAAGCAG CAGGGGTGTC CTGCAGCAGC TGGCTCCAAG CGTGCAAAAA GGCGAGAATG 
     1561 TCCACAAGCA CTCCCGCCTG GCAGAGGTCC TACAGCTGGG CTCAGACATC CTTCCCCAGT 
     1621 ACAAGCAAGA GGCACCAAAG ACTCCCCCTC ACATCATCTT ACATTATTGT GTTTTTAAGA 
     1681 CCACGTGGGA TTGGATCATC TTGATCTTGA CCTTCTATAC AGCCATCTTG GTCCCTTATA 
     1741 ATGTCTCCTT CAAAACCAGG CAGAATAATG TGGCCTGGCT GGTTGTTGAT AGCATCGTGG 
     1801 ATGTTATCTT TTTGGTGGAC ATTGTGCTCA ATTTTCATAC CACCTTTGTT GGACCAGCAG 
     1861 GGGAGGTGAT TTCTGACCCC AAACTTATCC GCATGAACTA CCTGAAGACG TGGTTTGTGA 
     1921 TTGACCTTCT GTCCTGTTTG CCATATGATG TCATCAACGC TTTTGAGAAC GTGGATGAGG 
     1981 TTAGTGCCTT TATGGGTGAT CCAGGGAAGA TTGGTTTTGC TGATCAGATT CCACCACCAC 
     2041 TGGAGGGGAG AGAGAGTCAG GGCATCAGCA GCCTGTTCAG CTCTCTAAAA GTTGTCCGGC 
     2101 TGCTCCGTCT TGGGCGAGTG GCCCGTAAGC TGGACCACTA CATTGAATAT GGAGCTGCTG 
     2161 TGCTGGTCCT GCTGGTGTGT GTGTTTGGGC TGGCTGCACA CTGGATGGCC TGCATCTGGT 
     2221 ACAGCATTGG GGACTATGAG ATCTTTGACG AGGACACCAA GACAATCCGC AACAACAGCT 
     2281 GGCTGTACCA ACTAGCGATG GACATTGGCA CCCCTTACCA GTTTAATGGG TCTGGCTCAG 
     2341 GGAAGTGGGA AGGTGGTCCC AGCAAGAATT CTGTCTACAT CTCCTCGTTG TATTTCACAA 
     2401 TGACCAGCCT CACCAGTGTG GGCTTTGGGA ACATCGCCCC ATCCACAGAC ATTGAGAAGA 
     2461 TCTTTGCAGT GGCCATCATG ATGATTGGCT CACTTCTCTA TGCCACCATC TTCGGGAATG 
     2521 TGACGACTAT TTTCCAACAG ATGTATGCCA ACACCAACAG ATACCATGAG ATGCTCAACA 
     2581 GTGTTCGGGA CTTCCTGAAG CTCTACCAGG TGCCAAAAGG ATTGAGTGAG CGAGTAATGG 
     2641 ATTATATTGT GTCCACTTGG TCCATGTCCA GAGGCATTGA CACAGAGAAG GTCCTGCAGA 
     2701 TCTGCCCCAA GGACATGAGA GCCGACATCT GCGTGCACCT GAACCGCAAG GTGTTCAAGG 
     2761 AGCACCCGGC CTTCCGGCTG GCCAGTGATG GCTGCCTCCG GGCACTGGCC ATGGAGTTCC 
     2821 AGACGGTGCA CTGTGCCCCA GGGGACCTCA TCTACCATGC AGGAGAGAGC GTTGACAGCC 
     2881 TCTGCTTTGT GGTTTCTGGC TCCCTGGAGG TGATCCAAGA TGATGAGGTG GTGGCCATTC 
     2941 TAGGAAAAGG AGACGTGTTT GGAGATGTGT TCTGGAAGGA AGCCACCCTT GCCCAGTCCT 
     3001 GTGCCAATGT TAGGGCCTTG ACCTACTGTG ATCTGCATGT GATCAAGCGG GATGCCCTGC 
     3061 AGAAAGTGCT GGAATTCTAC ACGGCCTTCT CCCATTCCTT CTCCCGGAAC CTGATTCTGA 
     3121 CGTACAACTT GAGGAAGAGG ATTGTGTTCC GGAAGATCAG CGATGTGAAA CGTGAAGAGG 
     3181 AAGAACGCAT GAAACGAAAG AATGAGGCCC CCCTGATCTT GCCCCCGGAC CACCCTGTCC 
     3241 GGCGCCTCTT CCAGAGATTC CGACAGCAGA AAGAGGCCAG GCTGGCAGCT GAGAGAGGGG 
     3301 GCCGGGACCT GGATGACCTA GATGTGGAGA AGGGCAATGT CCTTACAGAG CATGCCTCCG 
     3361 CCAACCACAG CCTCGTGAAG GCCAGCGTGG TCACCGTGCG TGAGAGTCCT GCCACGCCCG 
     3421 TATCCTTCCA GGCAGCCTCC ACCTCCGGGG TGCCAGACCA CGCAAAGCTA CAGGCGCCAG 
     3481 GGTCCGAGTG CCTGGGCCCC AAGGGGGGCG GGGGCGATTG TGCCAAGCGC AAAAGCTGGG 
     3541 CCCGCTTCAA AGATGCTTGC GGGAAGAGTG AGGACTGGAA CAAGGTGTCC AAGGCTGAGT 
     3601 CGATGGAGAC ACTTCCCGAG AGGACAAAAG CGTCAGGCGA GGCCACACTG AAGAAGACAG 
     3661 ACTCGTGTGA CAGTGGCATC ACCAAGAGCG ACTTGCGCCT GGACAACGTG GGTGAGGCCA 
     3721 GGAGTCCCCA GGATCGGAGT CCCATCCTGG CAGAGGTCAA GCATTCGTTC TACCCCATCC 
     3781 CTGAGCAGAC GCTGCAGGCC ACAGTCCTGG AGGTGAGGCA CGAGCTGAAG GAGGACATCA 
     3841 AGGCCTTAAA CGCCAAAATG ACCAATATTG AGAAACAGCT CTCTGAGATA CTCAGGATAT 
     3901 TAACTTCCAG AAGATCCTCT CAGTCTCCTC AGGAGTTGTT TGAAATATCG AGGCCACAGT 
     3961 CCCCAGAATC AGAGAGAGAC ATTTTTGGAG CCAGCACGCG TACGCGGCCG CTCGAGCAGA 
     4021 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4081 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4141 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4201 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4261 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4321 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4381 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4441 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4501 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4561 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4621 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4681 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4741 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4801 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4861 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4921 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4981 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5041 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5101 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5161 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5221 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5281 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5341 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5401 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5461 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5521 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5581 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5641 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5701 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5761 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5821 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5881 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5941 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6001 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6061 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6121 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6181 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6241 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6301 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6361 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6421 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6481 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6541 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6601 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6661 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6721 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6781 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6841 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6901 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6961 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7021 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7081 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7141 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7201 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7261 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7321 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7381 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7441 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7501 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7561 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7621 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7681 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7741 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7801 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//