LOCUS       RC218669                7761 bp    DNA     circular  SYN 
DEFINITION  MAP3K10 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 10 (MAP3K10)
ACCESSION   NM_002446
VERSION     NM_002446.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3916..4007 
                     /label="Myc-DDK"  
     misc_feature    4037..4622 
                     /label="PolyA signal"  
     misc_feature    4094..4113 
                     /label="Restriction Enzyme"  
     misc_feature    4898..5570 
                     /label="ColE1"  
     misc_feature    6000..6794 
                     /label="Kan/Neo"  
     misc_feature    6829..7131 
                     /label="SV40 ori"  
     misc_feature    7217..7694 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    3891..3898 
                     /label="Restriction Enzyme"  
     misc_feature    1029..3891 
                     /label="MAP3K10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GAGGAGGAGG AGGGGGCGGT GGCCAAGGAG TGGGGCACGA 
     1081 CCCCCGCGGG GCCCGTCTGG ACCGCGGTGT TCGACTACGA GGCGGCGGGC GACGAGGAGC 
     1141 TGACCCTGCG GAGGGGCGAT CGCGTCCAGG TGCTTTCCCA AGACTGTGCG GTGTCCGGCG 
     1201 ACGAGGGCTG GTGGACCGGG CAGCTCCCCA GCGGCCGCGT GGGCGTCTTC CCCAGCAACT 
     1261 ACGTGGCCCC CGGCGCCCCC GCTGCACCCG CGGGCCTCCA GCTGCCCCAG GAGATCCCCT 
     1321 TCCACGAGCT GCAGCTAGAG GAGATCATCG GTGTGGGGGG CTTTGGCAAG GTCTATCGGG 
     1381 CCCTGTGGCG TGGCGAGGAG GTGGCAGTCA AGGCCGCCCG GCTGGACCCT GAGAAGGACC 
     1441 CGGCAGTGAC AGCGGAGCAG GTGTGCCAGG AAGCCCGGCT CTTTGGAGCC CTGCAGCACC 
     1501 CCAACATAAT TGCCCTTAGG GGCGCCTGCC TCAACCCCCC ACACCTCTGC CTAGTGATGG 
     1561 AGTATGCCCG GGGTGGTGCA CTGAGCAGGG TGCTGGCAGG TCGCCGGGTG CCACCTCACG 
     1621 TGCTGGTCAA CTGGGCTGTG CAGGTGGCCC GGGGCATGAA CTACCTACAC AATGATGCCC 
     1681 CTGTGCCCAT CATCCACCGG GACCTCAAGT CCATCAACAT CCTGATCCTG GAGGCCATCG 
     1741 AGAACCACAA CCTCGCAGAC ACGGTGCTCA AGATCACGGA CTTCGGCCTC GCCCGCGAGT 
     1801 GGCACAAGAC CACCAAGATG AGCGCTGCGG GGACCTACGC CTGGATGGCG CCGGAGGTTA 
     1861 TCCGTCTCTC CCTCTTCTCC AAAAGCAGTG ATGTCTGGAG CTTCGGGGTG CTGCTGTGGG 
     1921 AGCTGCTGAC GGGGGAGGTC CCCTACCGTG AGATCGACGC CTTGGCCGTG GCGTATGGCG 
     1981 TGGCTATGAA TAAGCTGACG CTGCCCATTC CCTCCACGTG CCCCGAGCCC TTTGCCCGCC 
     2041 TCCTGGAGGA ATGCTGGGAC CCAGACCCCC ACGGGCGGCC AGATTTCGGT AGCATCTTGA 
     2101 AGCGGCTTGA AGTCATCGAA CAGTCAGCCC TGTTCCAGAT GCCACTGGAG TCCTTCCACT 
     2161 CGCTGCAGGA AGACTGGAAG CTGGAGATTC AGCACATGTT TGATGACCTT CGGACCAAGG 
     2221 AGAAGGAGCT TCGGAGCCGT GAGGAGGAGC TGCTGCGGGC GGCACAGGAG CAGCGCTTCC 
     2281 AGGAGGAGCA GCTGCGGCGG CGGGAGCAGG AGCTGGCAGA ACGTGAGATG GACATCGTGG 
     2341 AACGGGAGCT GCACCTGCTC ATGTGCCAGC TGAGCCAGGA GAAGCCCCGG GTCCGCAAGC 
     2401 GCAAGGGCAA CTTCAAGCGC AGCCGCCTGC TCAAGCTGCG GGAAGGCGGC AGCCACATCA 
     2461 GCCTGCCCTC TGGCTTTGAG CATAAGATCA CAGTCCAGGC CTCTCCAACT CTGGATAAGC 
     2521 GGAAAGGATC CGATGGGGCC AGCCCCCCTG CAAGCCCCAG CATCATCCCC CGGCTGAGGG 
     2581 CCATTCGCCT GACTCCCGTG GACTGTGGTG GCAGCAGCAG TGGCAGCAGC AGTGGAGGAA 
     2641 GTGGGACATG GAGCCGCGGT GGGCCCCCAA AGAAGGAAGA ACTGGTCGGG GGCAAGAAGA 
     2701 AGGGACGAAC GTGGGGGCCC AGCTCCACCC TGCAGAAGGA GCGGGTGGGA GGAGAGGAGA 
     2761 GGCTGAAGGG GCTGGGGGAA GGAAGCAAAC AGTGGTCATC AAGTGCCCCC AACCTGGGCA 
     2821 AGTCCCCCAA ACACACACCC ATCGCCCCTG GCTTTGCCAG CCTCAATGAG ATGGAGGAGT 
     2881 TCGCGGAGGC AGAGGATGGA GGCAGCAGCG TGCCCCCTTC CCCCTACTCG ACCCCGTCCT 
     2941 ACCTCTCAGT GCCACTGCCT GCCGAGCCCT CCCCGGGGGC GCGGGCGCCG TGGGAGCCGA 
     3001 CGCCGTCCGC GCCCCCCGCT CGGTGGGGAC ACGGCGCCCG GCGGCGCTGC GACCTGGCGC 
     3061 TGCTAGGCTG CGCCACGCTG CTGGGGGCTG TGGGCCTGGG CGCCGACGTG GCCGAGGCGC 
     3121 GCGCGGCCGA CGGTGAGGAG CAGCGGCGCT GGCTCGACGG CCTCTTCTTT CCCCGCGCCG 
     3181 GCCGCTTCCC GCGGGGCCTC AGCCCACCCG CGCGTCCCCA CGGCCGCCGC GAAGACGTGG 
     3241 GCCCCGGCCT GGGCCTGGCG CCCTCGGCCA CCCTCGTGTC GCTGTCGTCC GTGTCCGACT 
     3301 GCAACTCCAC GCGTTCACTG CTGCGCTCTG ACAGTGACGA GGCCGCACCG GCCGCGCCCT 
     3361 CCCCACCACC CTCCCCGCCC GCGCCCACAC CCACGCCCTC GCCCAGCACC AACCCCCTGG 
     3421 TGGACCTGGA GCTGGAGAGC TTCAAGAAGG ACCCCCGCCA GTCGCTCACG CCCACCCACG 
     3481 TCACGGCTGC ATGCGCTGTG AGCCGCGGGC ACCGGCGGAC GCCATCGGAC GGGGCGCTGG 
     3541 GGCAGCGGGG GCCGCCCGAG CCCGCGGGCC ATGGCCCTGG CCCTCGTGAC CTTCTGGACT 
     3601 TCCCCCGCCT GCCCGACCCC CAGGCCCTGT TCCCAGCCCG CCGCCGGCCC CCTGAGTTCC 
     3661 CAGGCCGCCC CACCACCCTG ACCTTTGCCC CGAGACCTCG GCCGGCTGCC AGTCGCCCCC 
     3721 GCTTGGACCC CTGGAAACTG GTCTCCTTCG GCCGGACACT CACCATCTCG CCTCCCAGCA 
     3781 GGCCAGACAC TCCGGAGAGC CCTGGGCCCC CCAGCGTGCA GCCCACACTG CTGGACATGG 
     3841 ACATGGAGGG GCAGAACCAA GACAGCACAG TGCCCCTGTG CGGGGCCCAC GGCTCCCACA 
     3901 GCGGACCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3961 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4021 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4081 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4141 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4201 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4261 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4321 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4381 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4441 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4501 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4561 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4621 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4681 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4741 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4801 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4861 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4921 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4981 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5041 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5101 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5161 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5221 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5281 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5341 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5401 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5461 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5521 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5581 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5641 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5701 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5761 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5821 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5881 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5941 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6001 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6061 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6121 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6181 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6241 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6301 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6361 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6421 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6481 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6541 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6601 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6661 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6721 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6781 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6841 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6901 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6961 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7021 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7081 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7141 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7201 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7261 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7321 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7381 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7441 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7501 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7561 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7621 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7681 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7741 CAAAAATTTA ACGCGAATTT T
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