LOCUS       RC218629                8889 bp    DNA     circular  SYN 
DEFINITION  WDR33 (Myc-DDK-tagged)-Human WD repeat domain 33 (WDR33), transcript variant 1
ACCESSION   NM_018383
VERSION     NM_018383.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5046..5137 
                     /label="Myc-DDK"  
     misc_feature    5167..5752 
                     /label="PolyA signal"  
     misc_feature    5224..5243 
                     /label="Restriction Enzyme"  
     misc_feature    6028..6700 
                     /label="ColE1"  
     misc_feature    7130..7924 
                     /label="Kan/Neo"  
     misc_feature    7959..8261 
                     /label="SV40 ori"  
     misc_feature    8347..8824 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5028..5034 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5028 
                     /label="WDR33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTACAGAA ATTGGTTCTC CTCCTCGTTT TTTCCATATG CCAAGGTTCC 
     1081 AGCACCAGGC ACCTCGACAG CTGTTTTATA AGCGACCTGA TTTTGCACAA CAGCAAGCAA 
     1141 TGCAACAGCT TACTTTTGAT GGAAAACGAA TGAGAAAAGC TGTGAACCGA AAAACCATAG 
     1201 ACTACAATCC ATCTGTAATT AAGTATTTGG AGAACAGAAT ATGGCAAAGA GACCAGAGAG 
     1261 ATATGCGGGC AATTCAGCCT GATGCAGGTT ATTACAATGA TCTGGTCCCA CCTATAGGAA 
     1321 TGTTGAATAA TCCTATGAAT GCAGTAACAA CAAAATTTGT TCGGACATCA ACAAATAAAG 
     1381 TAAAGTGTCC TGTATTTGTT GTTAGGTGGA CTCCAGAAGG AAGACGCTTG GTCACTGGAG 
     1441 CTTCTAGTGG GGAGTTTACC CTGTGGAATG GACTCACTTT CAATTTTGAA ACAATATTAC 
     1501 AGGCTCACGA CAGCCCAGTG AGGGCCATGA CGTGGTCACA TAATGACATG TGGATGTTGA 
     1561 CAGCAGACCA CGGAGGATAT GTGAAATATT GGCAGTCGAA CATGAACAAC GTCAAGATGT 
     1621 TCCAGGCACA TAAGGAGGCG ATTAGAGAGG CCAGTTTCTC ACCCACGGAT AATAAATTTG 
     1681 CTACATGCTC TGATGACGGC ACTGTTAGAA TCTGGGACTT TCTTCGTTGC CATGAGGAAA 
     1741 GAATTCTCCG AGGGCATGGT GCTGATGTGA AATGTGTAGA CTGGCATCCA ACCAAAGGGT 
     1801 TAGTTGTTTC AGGAAGTAAA GATAGTCAAC AGCCAATCAA GTTCTGGGAT CCCAAGACTG 
     1861 GGCAGAGTCT TGCAACACTT CATGCCCATA AAAACACAGT AATGGAAGTG AAATTAAACC 
     1921 TCAATGGCAA TTGGCTACTC ACAGCATCAC GTGATCATCT CTGTAAACTT TTTGATATCA 
     1981 GAAACCTAAA AGAAGAGCTT CAAGTCTTCC GAGGTCATAA GAAAGAAGCC ACAGCTGTGG 
     2041 CCTGGCATCC TGTTCATGAA GGACTTTTTG CCAGTGGAGG GTCTGATGGT TCTTTGTTAT 
     2101 TCTGGCATGT TGGGGTAGAG AAGGAAGTGG GTGGGATGGA GATGGCTCAC GAAGGGATGA 
     2161 TCTGGAGTCT GGCTTGGCAT CCTCTTGGGC ATATTCTCTG CTCAGGCTCA AATGACCATA 
     2221 CTAGCAAATT CTGGACTCGA AACCGACCAG GTGATAAAAT GCGAGATCGA TATAATCTAA 
     2281 ACCTTTTACC TGGAATGTCT GAAGATGGAG TAGAATATGA TGACCTCGAA CCTAATAGCC 
     2341 TGGCAGTAAT TCCAGGAATG GGAATACCAG AACAACTAAA ATTAGCTATG GAACAAGAAC 
     2401 AGATGGGGAA AGATGAATCA AATGAAATTG AAATGACAAT TCCAGGTTTA GATTGGGGAA 
     2461 TGGAGGAAGT GATGCAAAAG GATCAGAAAA AAGTACCTCA GAAGAAAGTT CCTTATGCAA 
     2521 AACCCATTCC TGCTCAGTTC CAGCAGGCTT GGATGCAAAA TAAAGTTCCA ATTCCTGCTC 
     2581 CAAATGAGGT GCTGAATGAC AGAAAAGAAG ACATTAAATT GGAAGAGAAG AAAAAAACAC 
     2641 AAGCAGAAAT TGAGCAAGAA ATGGCTACAT TACAATATAC TAACCCACAA CTTCTGGAGC 
     2701 AACTTAAAAT TGAAAGACTT GCACAGAAAC AAGTTGAGCA AATTCAGCCT CCTCCCTCAT 
     2761 CTGGCACCCC TCTCCTCGGA CCCCAGCCTT TTCCAGGACA AGGTCCAATG TCTCAGATTC 
     2821 CTCAAGGTTT TCAACAGCCC CATCCATCTC AGCAGATGCC AATGAACATG GCTCAAATGG 
     2881 GGCCTCCAGG TCCACAGGGA CAGTTTAGGC CTCCTGGACC CCAGGGACAA ATGGGACCAC 
     2941 AAGGTCCTCC ACTGCATCAG GGAGGTGGGG GGCCACAAGG ATTCATGGGA CCACAGGGGC 
     3001 CCCAGGGCCC GCCCCAGGGG TTGCCACGGC CTCAGGACAT GCATGGGCCC CAAGGAATGC 
     3061 AGAGGCATCC TGGACCTCAT GGCCCTTTGG GACCTCAAGG GCCACCTGGA CCACAAGGTA 
     3121 GTTCTGGTCC TCAAGGTCAT ATGGGTCCTC AGGGTCCACC TGGCCCACAG GGTCACATAG 
     3181 GCCCCCAAGG CCCGCCTGGC CCTCAGGGTC ACTTGGGCCC ACAGGGGCCT CCGGGTACTC 
     3241 AAGGTATGCA GGGACCACCT GGTCCCAGAG GAATGCAAGG GCCTCCTCAT CCTCATGGGA 
     3301 TCCAAGGCGG ACCAGGGTCT CAAGGGATCC AAGGTCCTGT GTCTCAGGGA CCTCTGATGG 
     3361 GATTGAATCC AAGAGGAATG CAGGGGCCTC CAGGCCCCCG GGAGAACCAG GGTCCTGCTC 
     3421 CCCAAGGGAT GATTATGGGC CACCCGCCTC AAGAGATGAG AGGACCTCAC CCTCCAGGTG 
     3481 GACTACTGGG ACACGGCCCT CAGGAAATGA GAGGTCCTCA GGAGATCCGA GGCATGCAGG 
     3541 GGCCTCCACC CCAAGGATCA ATGCTGGGAC CTCCCCAGGA ATTGCGAGGG CCTCCAGGCT 
     3601 CACAAAGTCA GCAGGGGCCG CCCCAGGGCT CTTTAGGACC TCCACCCCAG GGTGGCATGC 
     3661 AAGGACCCCC CGGACCTCAG GGACAGCAGA ACCCAGCAAG AGGGCCACAT CCATCTCAAG 
     3721 GGCCAATACC ATTCCAGCAA CAGAAAACGC CTCTGCTAGG TGATGGGCCC CGGGCCCCCT 
     3781 TCAACCAGGA AGGACAGAGC ACAGGCCCCC CACCCCTGAT ACCAGGCCTA GGGCAGCAGG 
     3841 GAGCACAAGG TCGCATTCCC CCTCTGAACC CCGGACAAGG ACCTGGCCCC AACAAAGGTG 
     3901 ACTCCCGCGG CCCTCCAAAC CATCACATGG GCCCGATGTC AGAGAGGCGG CATGAGCAGA 
     3961 GCGGCGGGCC TGAGCATGGG CCCGAGAGGG GGCCTTTCCG GGGTGGCCAG GACTGCAGGG 
     4021 GTCCCCCTGA TAGGCGTGGC CCTCACCCAG ACTTCCCCGA TGACTTCAGC AGACCAGATG 
     4081 ACTTCCACCC TGACAAGCGC TTTGGCCACC GGTTACGTGA ATTTGAGGGG CGAGGAGGAC 
     4141 CTTTACCGCA AGAAGAGAAG TGGAGGCGAG GGGGCCCTGG GCCTCCGTTT CCCCCTGATC 
     4201 ACAGGGAATT CAGCGAGGGG GATGGCCGGG GTGCAGCCCG AGGCCCTCCG GGGGCATGGG 
     4261 AAGGCCGCAG GCCCGGAGAT GAACGTTTCC CCCGGGATCC CGAGGACCCA CGTTTTCGAG 
     4321 GGCGCAGAGA AGAAAGTTTC AGAAGAGGAG CCCCGCCGAG GCATGAGGGC CGTGCTCCCC 
     4381 CCAGAGGAAG GGATGGTTTT CCTGGTCCTG AAGACTTTGG TCCAGAGGAG AATTTTGATG 
     4441 CTTCTGAGGA AGCGGCCCGA GGACGAGATC TCAGAGGTCG AGGTCGGGGT ACCCCACGAG 
     4501 GAGGAAGGAA GGGTTTACTT CCCACTCCTG ACGAGTTCCC TCGCTTTGAA GGAGGGCGGA 
     4561 AGCCAGATTC CTGGGATGGA AACAGAGAGC CTGGGCCAGG TCATGAACAT TTTCGTGATA 
     4621 CTCCCCGCCC TGATCATCCC CCTCACGACG GTCATTCCCC AGCCAGCAGA GAACGCTCCT 
     4681 CTTCTCTCCA AGGCATGGAC ATGGCATCCC TACCTCCCCG AAAGCGCCCC TGGCATGATG 
     4741 GCCCAGGCAC TTCTGAGCAC AGAGAGATGG AGGCCCCAGG AGGCCCTTCT GAAGACCGAG 
     4801 GAGGCAAAGG CCGAGGGGGC CCAGGACCTG CTCAGAGAGT GCCCAAATCT GGGCGTTCCA 
     4861 GCTCCTTAGA CGGAGAGCAC CACGATGGAT ACCACAGAGA TGAACCTTTT GGGGGCCCTC 
     4921 CAGGCAGTGG CACCCCTTCT CGAGGGGGCC GGAGTGGCAG TAACTGGGGT AGAGGGAGTA 
     4981 ACATGAACTC TGGCCCGCCG AGGCGAGGAG CTTCACGGGG TGGTGGAAGG GGTCGGACGC 
     5041 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5101 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5161 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5221 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5281 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5341 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5401 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5461 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5521 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5581 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5641 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5701 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5761 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5821 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5881 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5941 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6001 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6061 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6121 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6181 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6241 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6301 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6361 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6421 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6481 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6541 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6601 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6661 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6721 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6781 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6841 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6901 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6961 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7021 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7081 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7141 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7201 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7261 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7321 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7381 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7441 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7501 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7561 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7621 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7681 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7741 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7801 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7861 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7921 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7981 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8041 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8101 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8221 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8281 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8341 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8401 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8461 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8521 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8581 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8641 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8701 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8761 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8821 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8881 GCGAATTTT
//