LOCUS       RC218562                9483 bp    DNA     circular  SYN 
DEFINITION  SLIT1 (Myc-DDK-tagged)-Human slit homolog 1 (Drosophila) (SLIT1)
ACCESSION   NM_003061
VERSION     NM_003061.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5640..5731 
                     /label="Myc-DDK"  
     misc_feature    5761..6346 
                     /label="PolyA signal"  
     misc_feature    5818..5837 
                     /label="Restriction Enzyme"  
     misc_feature    6622..7294 
                     /label="ColE1"  
     misc_feature    7724..8518 
                     /label="Kan/Neo"  
     misc_feature    8553..8855 
                     /label="SV40 ori"  
     misc_feature    8941..9418 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5622..5628 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5622 
                     /label="SLIT1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGCTGACT CCCGGGTGGG GGTCCTCGGC GGGGCCGGTC CGGCCGGAGC 
     1081 TCTGGCTGCT GCTGTGGGCA GCCGCGTGGC GCCTGGGTGC CTCGGCGTGC CCCGCCCTCT 
     1141 GCACCTGCAC CGGAACCACG GTGGACTGCC ACGGCACGGG GCTGCAGGCC ATTCCCAAGA 
     1201 ATATACCTCG GAACACCGAG CGCCTGGAAC TCAATGGCAA CAACATCACT CGGATCCATA 
     1261 AGAATGACTT TGCGGGGCTC AAGCAGCTGC GGGTGCTGCA GCTGATGGAG AACCAGATTG 
     1321 GAGCAGTGGA ACGTGGTGCT TTTGATGACA TGAAGGAGCT GGAGCGGCTG CGACTGAACC 
     1381 GAAACCAGCT GCACATGTTA CCGGAACTGC TGTTCCAGAA CAACCAGGCT TTGTCAAGAC 
     1441 TGGACTTGAG TGAGAACGCC ATCCAGGCCA TCCCCAGGAA AGCTTTTCGG GGAGCTACGG 
     1501 ACCTTAAAAA TTTACGGCTG GACAAGAACC AGATCAGCTG CATTGAGGAA GGGGCCTTCC 
     1561 GTGCTCTGCG GGGGCTGGAG GTGCTGACCC TGAACAACAA CAATATCACC ACCATCCCCG 
     1621 TGTCCAGCTT CAACCATATG CCCAAGCTAC GGACCTTCCG CCTGCACTCC AACCACCTGT 
     1681 TTTGCGACTG CCACCTGGCC TGGCTCTCGC AGTGGCTGAG GCAGCGGCCA ACCATCGGGC 
     1741 TCTTCACCCA GTGCTCGGGC CCAGCCAGCC TGCGTGGCCT CAATGTGGCA GAGGTCCAGA 
     1801 AGAGTGAGTT CAGCTGCTCA GGCCAGGGAG AAGCGGGGCG CGTGCCCACC TGCACCCTGT 
     1861 CCTCCGGCTC CTGCCCGGCC ATGTGCACCT GCAGCAATGG CATCGTGGAC TGTCGTGGAA 
     1921 AAGGCCTCAC TGCCATCCCG GCCAACCTGC CCGAGACCAT GACGGAGATA CGCCTGGAGC 
     1981 TGAACGGCAT CAAGTCCATC CCTCCTGGAG CCTTCTCACC CTACAGAAAG CTACGGAGGA 
     2041 TAGACCTGAG CAACAATCAG ATCGCTGAGA TTGCACCCGA CGCCTTCCAG GGCCTCCGCT 
     2101 CCCTGAACTC GCTGGTCCTC TATGGAAACA AGATCACAGA CCTCCCCCGT GGTGTGTTTG 
     2161 GAGGCCTATA CACCCTACAG CTCCTGCTCC TGAATGCCAA CAAGATCAAC TGCATCCGGC 
     2221 CCGATGCCTT CCAGGACCTG CAGAACCTCT CACTGCTCTC CCTGTATGAC AACAAGATCC 
     2281 AGAGCCTCGC CAAGGGCACT TTCACCTCCC TGCGGGCCAT CCAGACTCTG CACCTGGCCC 
     2341 AGAACCCTTT CATTTGCGAC TGTAACCTCA AGTGGCTGGC AGACTTCCTG CGCACCAATC 
     2401 CCATCGAGAC GAGTGGTGCC CGCTGTGCCA GTCCCCGGCG CCTCGCCAAC AAGCGCATCG 
     2461 GGCAGATCAA GAGCAAGAAG TTCCGGTGCT CAGCCAAAGA GCAGTACTTC ATTCCAGGCA 
     2521 CGGAGGATTA CCAGCTGAAC AGCGAGTGCA ACAGCGACGT GGTCTGTCCC CACAAGTGCC 
     2581 GCTGTGAGGC CAACGTGGTG GAGTGCTCCA GCCTGAAGCT CACCAAGATC CCTGAGCGCA 
     2641 TCCCCCAGTC CACGGCAGAA CTGCGATTGA ATAACAATGA GATTTCCATC CTGGAGGCCA 
     2701 CTGGGATGTT TAAAAAACTT ACACATCTGA AGAAAATCAA TCTGAGCAAC AACAAGGTGT 
     2761 CAGAAATTGA AGATGGGGCC TTCGAGGGCG CAGCCTCTGT GAGCGAGCTG CACCTAACTG 
     2821 CCAACCAGCT GGAGTCCATC CGGAGCGGCA TGTTCCGGGG TCTGGATGGC TTGAGGACCC 
     2881 TAATGCTGCG GAACAACCGC ATCAGCTGCA TCCACAACGA CAGCTTCACG GGCCTGCGCA 
     2941 ACGTCCGGCT CCTCTCGCTC TACGACAACC AGATCACCAC CGTATCCCCA GGAGCCTTCG 
     3001 ACACCCTCCA GTCCCTCTCC ACACTGAATC TCCTGGCCAA CCCTTTCAAC TGCAACTGCC 
     3061 AGCTGGCCTG GCTAGGAGGC TGGCTACGGA AGCGCAAGAT CGTGACGGGG AACCCGCGAT 
     3121 GCCAGAACCC TGACTTTTTG CGGCAGATTC CCCTGCAGGA CGTGGCCTTC CCTGACTTCA 
     3181 GGTGTGAGGA AGGCCAGGAG GAGGGGGGCT GCCTGCCCCG CCCACAGTGC CCACAGGAGT 
     3241 GCGCCTGCCT GGACACCGTG GTCCGATGCA GCAACAAGCA CCTGCGGGCC CTGCCCAAGG 
     3301 GCATTCCCAA GAATGTCACA GAACTCTATT TGGACGGGAA CCAGTTCACG CTGGTTCCGG 
     3361 GACAGCTGTC TACCTTCAAG TACCTGCAGC TCGTGGACCT GAGCAACAAC AAGATCAGTT 
     3421 CCTTAAGCAA TTCCTCCTTC ACCAACATGA GCCAGCTGAC CACTCTGATC CTCAGCTACA 
     3481 ATGCCCTGCA GTGCATCCCG CCTTTGGCCT TCCAGGGACT CCGCTCCCTG CGCCTGCTGT 
     3541 CTCTCCACGG CAATGACATC TCCACCCTCC AAGAGGGCAT CTTTGCAGAC GTGACCTCCC 
     3601 TGTCTCACCT GGCCATTGGT GCCAACCCCC TATACTGTGA CTGCCACCTC CGCTGGCTGT 
     3661 CCAGCTGGGT GAAGACTGGC TACAAGGAAC CGGGCATTGC TCGTTGTGCT GGGCCCCAGG 
     3721 ACATGGAGGG CAAGCTGCTC CTCACCACGC CTGCCAAGAA GTTTGAATGC CAAGGTCCTC 
     3781 CAACGCTGGC TGTCCAGGCC AAGTGTGATC TCTGCTTGTC CAGTCCGTGC CAGAACCAGG 
     3841 GCACCTGCCA CAACGACCCC CTTGAGGTGT ACAGGTGCGC CTGCCCCAGC GGCTATAAGG 
     3901 GTCGAGACTG TGAGGTGTCC CTGAACAGCT GTTCCAGTGG CCCCTGTGAA AATGGGGGCA 
     3961 CCTGCCATGC ACAGGAGGGC GAGGATGCCC CGTTCACGTG CTCCTGTCCC ACCGGCTTTG 
     4021 AAGGACCAAC CTGTGGGGTG AACACAGATG ACTGTGTGGA TCATGCCTGT GCCAATGGGG 
     4081 GCGTCTGTGT GGATGGTGTG GGCAACTACA CCTGCCAGTG CCCCCTGCAG TATGAGGGAA 
     4141 AGGCCTGTGA GCAGCTGGTG GACTTGTGCT CTCCGGATCT GAACCCATGT CAACACGAGG 
     4201 CCCAGTGTGT GGGCACCCCG GATGGGCCCA GGTGTGAGTG CATGCCAGGT TATGCAGGTG 
     4261 ACAACTGCAG TGAGAACCAG GATGACTGCA GGGACCACCG CTGCCAGAAT GGGGCCCAGT 
     4321 GTATGGATGA AGTCAACAGC TACTCCTGCC TCTGTGCTGA GGGCTACAGT GGACAGCTCT 
     4381 GTGAGATCCC TCCCCATCTG CCTGCCCCCA AGAGCCCCTG TGAGGGGACT GAGTGCCAGA 
     4441 ATGGGGCCAA CTGTGTGGAC CAGGGCAACA GGCCTGTGTG CCAGTGCCTC CCAGGCTTCG 
     4501 GTGGCCCTGA GTGTGAGAAG TTGCTCAGTG TCAACTTTGT GGATCGGGAC ACTTACCTGC 
     4561 AGTTCACTGA CCTGCAAAAC TGGCCACGGG CCAACATCAC GTTGCAGGTC TCCACGGCAG 
     4621 AGGACAATGG GATCCTTCTG TACAACGGGG ACAACGACCA CATTGCAGTT GAGCTGTACC 
     4681 AGGGCCATGT GCGTGTCAGC TACGACCCAG GCAGCTACCC CAGCTCTGCC ATCTACAGTG 
     4741 CTGAGACGAT CAACGATGGG CAATTCCACA CCGTTGAGCT GGTTGCCTTT GACCAGATGG 
     4801 TGAATCTCTC CATTGATGGC GGGAGCCCCA TGACCATGGA CAACTTTGGC AAACATTACA 
     4861 CGCTCAACAG CGAGGCGCCA CTCTATGTGG GAGGGATGCC CGTGGATGTC AACTCAGCTG 
     4921 CCTTCCGCCT GTGGCAGATC CTCAACGGCA CCGGCTTCCA CGGTTGCATC CGAAACCTGT 
     4981 ACATCAACAA CGAGCTGCAG GACTTCACCA AGACGCAGAT GAAGCCAGGC GTGGTGCCAG 
     5041 GCTGCGAACC CTGCCGCAAG CTCTACTGCC TGCATGGCAT CTGCCAGCCC AATGCCACCC 
     5101 CAGGGCCCAT GTGCCACTGC GAGGCTGGCT GGGTGGGCCT GCACTGTGAC CAGCCCGCTG 
     5161 ACGGCCCCTG CCATGGCCAC AAGTGTGTCC ATGGGCAATG CGTGCCCCTC GACGCTCTTT 
     5221 CCTACAGCTG CCAGTGCCAG GATGGGTACT CGGGGGCACT GTGCAACCAG GCCGGGGCCC 
     5281 TGGCAGAGCC CTGCAGAGGC CTGCAGTGCC TGCATGGCCA CTGCCAGGCC TCAGGCACCA 
     5341 AGGGGGCACA CTGTGTGTGT GACCCCGGCT TTTCGGGCGA GCTGTGTGAG CAAGAGTCCG 
     5401 AGTGCCGGGG GGACCCTGTC CGGGACTTTC ACCAGGTCCA GAGGGGCTAT GCCATCTGCC 
     5461 AGACCACGCG CCCCCTGTCA TGGGTGGAGT GCCGGGGCTC GTGCCCAGGC CAGGGCTGCT 
     5521 GCCAGGGCCT TCGGCTGAAG CGGAGGAAGT TCACCTTTGA GTGCAGCGAT GGGACCTCTT 
     5581 TTGCCGAGGA GGTGGAAAAG CCCACCAAGT GTGGCTGTGC CCTCTGCGCA ACGCGTACGC 
     5641 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5701 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5761 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5821 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5881 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5941 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     6001 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     6061 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     6121 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     6181 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     6241 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     6301 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     6361 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     6421 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     6481 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     6541 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     6601 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6661 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6721 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6781 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6841 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6901 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6961 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     7021 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     7081 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     7141 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     7201 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     7261 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     7321 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     7381 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     7441 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     7501 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     7561 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     7621 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7681 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7741 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7801 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7861 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7921 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7981 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     8041 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     8101 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     8161 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     8221 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     8281 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     8341 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     8401 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     8461 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     8521 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     8581 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     8641 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8701 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8761 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8821 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8881 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8941 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     9001 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     9061 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     9121 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     9181 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     9241 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     9301 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     9361 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     9421 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     9481 TTT
//