LOCUS       RC218516                8214 bp    DNA     circular  SYN 
DEFINITION  LAMC2 (Myc-DDK-tagged)-Human laminin, gamma 2 (LAMC2), transcript variant 2
ACCESSION   NM_018891
VERSION     NM_018891.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4371..4462 
                     /label="Myc-DDK"  
     misc_feature    4492..5077 
                     /label="PolyA signal"  
     misc_feature    4549..4568 
                     /label="Restriction Enzyme"  
     misc_feature    5353..6025 
                     /label="ColE1"  
     misc_feature    6455..7249 
                     /label="Kan/Neo"  
     misc_feature    7284..7586 
                     /label="SV40 ori"  
     misc_feature    7672..8149 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4353..4359 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4353 
                     /label="LAMC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGCGCTC TGGCTGGGCT GCTGCCTCTG CTTCTCGCTC CTCCTGCCCG 
     1081 CAGCCCGGGC CACCTCCAGG AGGGAAGTCT GTGATTGCAA TGGGAAGTCC AGGCAGTGTA 
     1141 TCTTTGATCG GGAACTTCAC AGACAAACTG GTAATGGATT CCGCTGCCTC AACTGCAATG 
     1201 ACAACACTGA TGGCATTCAC TGCGAGAAGT GCAAGAATGG CTTTTACCGG CACAGAGAAA 
     1261 GGGACCGCTG TTTGCCCTGC AATTGTAACT CCAAAGGTTC TCTTAGTGCT CGATGTGACA 
     1321 ACTCCGGACG GTGCAGCTGT AAACCAGGTG TGACAGGAGC CAGATGCGAC CGATGTCTGC 
     1381 CAGGCTTCCA CATGCTCATG GATGCGGGGT GCACCCAAGA CCAGAGACTG CTAGACTCCA 
     1441 AGTGTGACTG TGACCCAGCT GGCATCGCAG GGCCCTGTGA CGCGGGCCGC TGTGTCTGCA 
     1501 AGCCAGCTGT CACTGGAGAA CGCTGTGATA GGTGTCGATC AGGTTACTAT AATCTGGATG 
     1561 GGGGGAACCC TGAGGGCTGT ACCCAGTGTT TCTGCTATGG GCATTCAGCC AGCTGCCGCA 
     1621 GCTCTGCAGA ATACAGTGTC CATAAGATCA CCTCTACCTT TCATCAAGAT GTTGATGGCT 
     1681 GGAAGGCTGT CCAACGAAAT GGGTCTCCTG CAAAGCTCCA ATGGTCACAG CGCCATCAAG 
     1741 ATGTGTTTAG CTCAGCCCAA CGACTAGACC CTGTCTATTT TGTGGCTCCT GCCAAATTTC 
     1801 TTGGGAATCA ACAGGTGAGC TATGGTCAAA GCCTGTCCTT TGACTACCGT GTGGACAGAG 
     1861 GAGGCAGACA CCCATCTGCC CATGATGTGA TTCTGGAAGG TGCTGGTCTA CGGATCACAG 
     1921 CTCCCTTGAT GCCACTTGGC AAGACACTGC CTTGTGGGCT CACCAAGACT TACACATTCA 
     1981 GGTTAAATGA GCATCCAAGC AATAATTGGA GCCCCCAGCT GAGTTACTTT GAGTATCGAA 
     2041 GGTTACTGCG GAATCTCACA GCCCTCCGCA TCCGAGCTAC ATATGGAGAA TACAGTACTG 
     2101 GGTACATTGA CAATGTGACC CTGATTTCAG CCCGCCCTGT CTCTGGAGCC CCAGCACCCT 
     2161 GGGTTGAACA GTGTATATGT CCTGTTGGGT ACAAGGGGCA ATTCTGCCAG GATTGTGCTT 
     2221 CTGGCTACAA GAGAGATTCA GCGAGACTGG GGCCTTTTGG CACCTGTATT CCTTGTAACT 
     2281 GTCAAGGGGG AGGGGCCTGT GATCCAGACA CAGGAGATTG TTATTCAGGG GATGAGAATC 
     2341 CTGACATTGA GTGTGCTGAC TGCCCAATTG GTTTCTACAA CGATCCGCAC GACCCCCGCA 
     2401 GCTGCAAGCC ATGTCCCTGT CATAACGGGT TCAGCTGCTC AGTGATGCCG GAGACGGAGG 
     2461 AGGTGGTGTG CAATAACTGC CCTCCCGGGG TCACCGGTGC CCGCTGTGAG CTCTGTGCTG 
     2521 ATGGCTACTT TGGGGACCCC TTTGGTGAAC ATGGCCCAGT GAGGCCTTGT CAGCCCTGTC 
     2581 AATGCAACAA CAATGTGGAC CCCAGTGCCT CTGGGAATTG TGACCGGCTG ACAGGCAGGT 
     2641 GTTTGAAGTG TATCCACAAC ACAGCCGGCA TCTACTGCGA CCAGTGCAAA GCAGGCTACT 
     2701 TCGGGGACCC ATTGGCTCCC AACCCAGCAG ACAAGTGTCG AGCTTGCAAC TGTAACCCCA 
     2761 TGGGCTCAGA GCCTGTAGGA TGTCGAAGTG ATGGCACCTG TGTTTGCAAG CCAGGATTTG 
     2821 GTGGCCCCAA CTGTGAGCAT GGAGCATTCA GCTGTCCAGC TTGCTATAAT CAAGTGAAGA 
     2881 TTCAGATGGA TCAGTTTATG CAGCAGCTTC AGAGAATGGA GGCCCTGATT TCAAAGGCTC 
     2941 AGGGTGGTGA TGGAGTAGTA CCTGATACAG AGCTGGAAGG CAGGATGCAG CAGGCTGAGC 
     3001 AGGCCCTTCA GGACATTCTG AGAGATGCCC AGATTTCAGA AGGTGCTAGC AGATCCCTTG 
     3061 GTCTCCAGTT GGCCAAGGTG AGGAGCCAAG AGAACAGCTA CCAGAGCCGC CTGGATGACC 
     3121 TCAAGATGAC TGTGGAAAGA GTTCGGGCTC TGGGAAGTCA GTACCAGAAC CGAGTTCGGG 
     3181 ATACTCACAG GCTCATCACT CAGATGCAGC TGAGCCTGGC AGAAAGTGAA GCTTCCTTGG 
     3241 GAAACACTAA CATTCCTGCC TCAGACCACT ACGTGGGGCC AAATGGCTTT AAAAGTCTGG 
     3301 CTCAGGAGGC CACAAGATTA GCAGAAAGCC ACGTTGAGTC AGCCAGTAAC ATGGAGCAAC 
     3361 TGACAAGGGA AACTGAGGAC TATTCCAAAC AAGCCCTCTC ACTGGTGCGC AAGGCCCTGC 
     3421 ATGAAGGAGT CGGAAGCGGA AGCGGTAGCC CGGACGGTGC TGTGGTGCAA GGGCTTGTGG 
     3481 AAAAATTGGA GAAAACCAAG TCCCTGGCCC AGCAGTTGAC AAGGGAGGCC ACTCAAGCGG 
     3541 AAATTGAAGC AGATAGGTCT TATCAGCACA GTCTCCGCCT CCTGGATTCA GTGTCTCGGC 
     3601 TTCAGGGAGT CAGTGATCAG TCCTTTCAGG TGGAAGAAGC AAAGAGGATC AAACAAAAAG 
     3661 CGGATTCACT CTCAAGCCTG GTAACCAGGC ATATGGATGA GTTCAAGCGT ACACAGAAGA 
     3721 ATCTGGGAAA CTGGAAAGAA GAAGCACAGC AGCTCTTACA GAATGGAAAA AGTGGGAGAG 
     3781 AGAAATCAGA TCAGCTGCTT TCCCGTGCCA ATCTTGCTAA AAGCAGAGCA CAAGAAGCAC 
     3841 TGAGTATGGG CAATGCCACT TTTTATGAAG TTGAGAGCAT CCTTAAAAAC CTCAGAGAGT 
     3901 TTGACCTGCA GGTGGACAAC AGAAAAGCAG AAGCTGAAGA AGCCATGAAG AGACTCTCCT 
     3961 ACATCAGCCA GAAGGTTTCA GATGCCAGTG ACAAGACCCA GCAAGCAGAA AGAGCCCTGG 
     4021 GGAGCGCTGC TGCTGATGCA CAGAGGGCAA AGAATGGGGC CGGGGAGGCC CTGGAAATCT 
     4081 CCAGTGAGAT TGAACAGGAG ATTGGGAGTC TGAACTTGGA AGCCAATGTG ACAGCAGATG 
     4141 GAGCCTTGGC CATGGAAAAG GGACTGGCCT CTCTGAAGAG TGAGATGAGG GAAGTGGAAG 
     4201 GAGAGCTGGA AAGGAAGGAG CTGGAGTTTG ACACGAATAT GGATGCAGTA CAGATGGTGA 
     4261 TTACAGAAGC CCAGAAGGTT GATACCAGAG CCAAGAACGC TGGGGTTACA ATCCAAGACA 
     4321 CACTCAACAC ATTAGACGGC CTCCTGCATC TGATGGGTAT GACGCGTACG CGGCCGCTCG 
     4381 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4441 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4501 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4561 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4621 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4681 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4741 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4801 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4861 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4921 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4981 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5041 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5101 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5161 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5221 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5281 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5341 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5401 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5461 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5521 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5581 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5641 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5701 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5761 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5821 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5881 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5941 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6001 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6061 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6121 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6181 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6241 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6301 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6361 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6421 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6481 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6541 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6601 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6661 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6721 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6781 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6841 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6901 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6961 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7021 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7081 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7141 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7201 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7261 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7321 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7381 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7441 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7501 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7621 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7681 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7741 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7801 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7861 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7921 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7981 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8041 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8101 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8161 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//