LOCUS       RC218508                8904 bp    DNA     circular  SYN 
DEFINITION  MYO3B (Myc-DDK-tagged)-Human myosin IIIB (MYO3B), transcript variant 2
ACCESSION   NM_138995
VERSION     NM_138995.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5061..5152 
                     /label="Myc-DDK"  
     misc_feature    5182..5767 
                     /label="PolyA signal"  
     misc_feature    5239..5258 
                     /label="Restriction Enzyme"  
     misc_feature    6043..6715 
                     /label="ColE1"  
     misc_feature    7145..7939 
                     /label="Kan/Neo"  
     misc_feature    7974..8276 
                     /label="SV40 ori"  
     misc_feature    8362..8839 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5043..5049 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5043 
                     /label="MYO3B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAACATCTG TATGGATTAT TTCACTATAA TCCTATGATG CTTGGACTTG 
     1081 AATCACTTCC AGATCCCACA GACACCTGGG AAATTATAGA GACCATTGGT AAAGGCACCT 
     1141 ATGGCAAAGT CTACAAGGTA ACTAACAAGA GAGATGGGAG CCTGGCTGCA GTGAAAATTC 
     1201 TGGATCCAGT CAGTGATATG GATGAAGAAA TTGAGGCAGA ATACAACATT TTGCAGTTCC 
     1261 TTCCTAATCA TCCCAATGTT GTAAAGTTTT ATGGGATGTT TTACAAAGCG GATCACTGTG 
     1321 TAGGGGGACA GCTGTGGCTG GTCCTGGAGC TGTGTAATGG GGGCTCAGTC ACTGAGCTTG 
     1381 TCAAAGGTCT ACTCAGATGT GGCCAGCGGT TGGATGAAGC AATGATCTCA TACATCTTGT 
     1441 ACGGGGCCCT CTTGGGCCTT CAGCATTTGC ACAACAACCG AATCATCCAC CGTGATGTGA 
     1501 AGGGGAATAA CATTCTTCTG ACAACAGAAG GAGGAGTTAA GCTCGTTGAC TTTGGTGTTT 
     1561 CAGCTCAACT CACCAGTACA CGTCTGCGGA GAAACACATC TGTTGGCACC CCATTCTGGA 
     1621 TGGCCCCTGA GGTCATTGCC TGTGAGCAGC AGTATGACTC TTCCTATGAC GCTCGCTGTG 
     1681 ACGTCTGGTC CTTGGGGATC ACAGCTATTG AACTGGGGGA TGGAGACCCT CCCCTCTTTG 
     1741 ACATGCATCC TGTGAAAACA CTCTTTAAGA TTCCAAGAAA TCCTCCACCT ACTTTACTTC 
     1801 ATCCAGAAAA ATGGTGTGAA GAATTCAACC ACTTTATTTC ACAGTGTCTT ATTAAGGATT 
     1861 TTGAAAGGCG ACCTTCCGTC ACACATCTCC TTGACCACCC ATTTATTAAA GGAGTACATG 
     1921 GAAAAGTTCT GTTTCTGCAA AAACAGCTGG CCAAGGTTCT CCAAGACCAG AAGCATCAAA 
     1981 ATCCTGTTGC TAAAACCAGG CATGAGAGGA TGCATACCAG AAGACCTTAT CATGTGGAAG 
     2041 ATGCTGAAAA ATACTGCCTT GAGGATGATT TGGTCAACCT AGAGGTTCTG GATGAGGATA 
     2101 CAATTATCCA TCAGTTGCAG AAACGTTATG CAGACTTGCT AATTTACACA TATGTTGGAG 
     2161 ACATCTTAAT TGCCTTAAAC CCCTTCCAGA ATCTAAGCAT ATACTCTCCA CAGTTTTCCA 
     2221 GACTTTATCA TGGGGTGAAA CGCGCCTCCA ATCCCCCCCA CATATTTGCA TCAGCAGATG 
     2281 CTGCTTACCA GTGCATGGTT ACTCTCAGCA AAGACCAGTG CATTGTCATC AGCGGAGAGA 
     2341 GTGGCTCTGG GAAGACAGAA AGCGCCCACC TGATTGTTCA ACATTTGACT TTCTTGGGAA 
     2401 AGGCCAATAA TCAGACCTTG AGAGAGAAAA TTCTACAAGT CAACTCCCTG GTGGAAGCCT 
     2461 TTGGGAACTC ATGCACTGCC ATCAATGACA ACTCGAGCCG TTTTGGAAAA TATCTGGAAA 
     2521 TGATGTTTAC ACCAACTGGA GTTGTGATGG GGGCAAGAAT CTCTGAATAT CTCCTGGAAA 
     2581 AATCCAGAGT TATAAAACAG GCAGCGAGAG AGAAAAATTT TCATATATTT TACTATATTT 
     2641 ATGCTGGTCT TCATCACCAA AAGAAGCTTT CTGATTTCAG ACTTCCTGAG GAAAAACCTC 
     2701 CTAGGTACAT AGCTGATGAA ACTGGAAGGG TGATGCACGA CATAACTTCC AAGGAGTCTT 
     2761 ACAGAAGACA ATTCGAAGCA ATTCAGCATT GCTTCAGGAT TATAGGGTTC ACGGACAAAG 
     2821 AGGTGCACTC AGTGTACAGA ATTTTGGCTG GGATTTTGAA TATTGGGAAC ATTGAGTTCG 
     2881 CAGCTATTTC CTCTCAACAT CAGACTGATA AAAGTGAGGT GCCCAATGCT GAAGCTTTGC 
     2941 AAAATGCTGC CTCTGTTCTG TGCATTAGCC CTGAAGAGCT CCAGGAGGCC CTCACCTCCC 
     3001 ACTGTGTGGT CACCCGGGGC GAGACCATCA TCCGTGCCAA CACTGTAGAC AGGGCTGCGG 
     3061 ACGTTCGAGA CGCCATGTCC AAAGCCCTGT ATGGGAGGCT CTTCAGCTGG ATTGTGAATC 
     3121 GCATTAATAC ACTCCTGCAG CCAGACGAAA ACATATGTAG TGCAGGAGGT GGAATGAATG 
     3181 TGGGGATCTT GGATATCTTT GGATTCGAGA ATTTTCAGAG AAATTCATTT GAGCAGCTCT 
     3241 GCATAAACAT CGCCAATGAG CAAATCCAGT ACTATTTCAA TCAGCATGTT TTTGCTCTTG 
     3301 AGCAGATGGA ATATCAGAAT GAAGGCATTG ATGCTGTACC CGTGGAATAT GAGGACAACC 
     3361 GCCCGCTCTT GGACATGTTC CTCCAGAAAC CCCTGGGACT GCTTGCACTT TTGGATGAGG 
     3421 AAAGTCGGTT TCCCCAAGCA ACTGACCAGA CCCTGGTTGA TAAATTTGAA GATAATCTAC 
     3481 GATGCAAATA CTTCTGGAGG CCCAAAGGAG TGGAACTGTG CTTTGGCATT CAGCATTATG 
     3541 CTGGAAAGGT ATTATATGAT GCTTCTGGGG TTCTTGAGAA AAATAGAGAC ACTCTCCCTG 
     3601 CCGATGTGGT TGTGGTCCTG AGAACGTCAG AAAACATGCT TCTTCAGCAG CTCTTCTCAA 
     3661 TCCCTCTGAC CAAAACAGGT AATTTGGCCC AGACAAGAGC TAGGATAACA GTGGCCTCAA 
     3721 GTTCTTTGCC TCCACATTTC AGTGCTGGGA AAGCCAAGGT GGACACTCTG GAGGTGATAC 
     3781 GGCATCCGGA AGAAACCACC AACATGAAGA GGCAAACTGT GGCTTCTTAC TTCCGGTATT 
     3841 CTCTGATGGA CCTGCTCTCC AAAATGGTGG TTGGACAGCC CCACTTTGTG CGCTGCATTA 
     3901 AACCCAATGA TGACCGAGAG GCCCTGCAGT TCTCTCGAGA GAGGGTGCTG GCCCAGCTCC 
     3961 GCTCCACAGG GATTCTGGAG ACAGTCAGCA TCCGCCGCCA GGGCTATTCC CACCGCATCC 
     4021 TTTTTGAAGA ATTTGTGAAA AGGTATTATT ACTTGGCATT CACAGCACAT CAAACACCTC 
     4081 TTGCTAGCAA AGAGAGCTGT GTGGCTATCT TGGAAAAGTC CAGATTAGAT CACTGGGTGC 
     4141 TGGGAAAAAC AAAGGTTTTT CTCAAATATT ACCATGTTGA GCAATTAAAT TTGCTGCTTC 
     4201 GAGAAGTCAT AGGCAGAGTG GTTGTGCTGC AGGCATATAC CAAGGGGTGG CTTGGAGCCA 
     4261 GGAGATACAA AAAGGTCAGA GAGAAGAGAG AGAAGGGAGC CATTGCCATC CAGTCAGCCT 
     4321 GGAGAGGATA TGATGCTCGG AGGAAATTTA AGAAAATAAG CAACAGAAGG AATGAGTCTG 
     4381 CTGCTCATAA TCAAGCAGGG GACACTTCAA ACCAAAGCAG TGGGCCACAT TCCCCCGTCG 
     4441 CAGCAGGTAC GAGGGGAAGT GCCGAGGTTC AAGACTGCAG CGAGCCTGGT GACCATAAAG 
     4501 TTCTCAGGGG CTCTGTACAT CGTAGGAGCC ATTCACAAGC AGAATCCAAC AATGGCCGTA 
     4561 CACAGACTTC AAGCAACTCT CCTGCTGTCA CAGAGAAAAA TGGGCATTCA CAAGCCCAGA 
     4621 GTTCTCCAAA AGGGTGCGAT ATCTTCGCAG GACATGCAAA CAAGCACTCG GTTTCTGGGA 
     4681 CTGATTTGCT GTCTTCTCGG ATATGCCATC CTGCTCCAGA TCAGCAAGGA TTGAGTCTCT 
     4741 GGGGAGCCCC TCAAAAGCCT GGTTCAGAAA ATGGTCTTGC ACAGAAGCAT CGAACACCTC 
     4801 GCCGACGATG TCAGCAGCCC AAAATGCTGA GTAGCCCTGA GGACACCATG TACTATAACC 
     4861 AGTTAAATGG AACTCTAGAA TATCAAGGGA GCAAGAGGAA GCCAAGAAAA CTTGGCCAAA 
     4921 TCAAAGTACT TGATGGGGAA GATGAATATT ACAAATCTCT GTCACCAGTG GACTGTATCC 
     4981 CTGAGGAGAA CAACTCAGCC CACCCTTCCT TTTTTTCTTC ATCCTCAAAA GGAGACTCTT 
     5041 TTGCTCAACA TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5101 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5161 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5221 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5281 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5341 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5401 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5461 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5521 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5581 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5641 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5701 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5761 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5821 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5881 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5941 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6001 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6061 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6121 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6181 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6241 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6301 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6361 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6421 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6481 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6541 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6601 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6661 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6721 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6781 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6841 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6901 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6961 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7021 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7081 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7141 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7201 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7261 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7321 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7381 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7441 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7501 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7561 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7621 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7681 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7741 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7801 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7861 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7921 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7981 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8041 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8101 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8161 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8221 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8281 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8341 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8401 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8461 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8521 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8581 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8641 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8701 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8761 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8821 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8881 TAACAAAAAT TTAACGCGAA TTTT
//