LOCUS       RC218496                7902 bp    DNA     circular  SYN 
DEFINITION  SALL2 (Myc-DDK-tagged)-Human sal-like 2 (Drosophila) (SALL2)
ACCESSION   NM_005407
VERSION     NM_005407.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4059..4150 
                     /label="Myc-DDK"  
     misc_feature    4180..4765 
                     /label="PolyA signal"  
     misc_feature    4237..4256 
                     /label="Restriction Enzyme"  
     misc_feature    5041..5713 
                     /label="ColE1"  
     misc_feature    6143..6937 
                     /label="Kan/Neo"  
     misc_feature    6972..7274 
                     /label="SV40 ori"  
     misc_feature    7360..7837 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4041..4047 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4041 
                     /label="SALL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTCGGCGA AAGCAGCGGA AACCCCAACA GTTAATCTCG GACTGCGAAG 
     1081 GTCCCAGCGC GTCTGAGAAC GGTGATGCTA GCGAGGAGGA TCACCCCCAA GTCTGTGCCA 
     1141 AGTGCTGCGC ACAATTCACT GACCCAACTG AATTCCTCGC CCACCAGAAC GCATGTTCTA 
     1201 CTGACCCTCC TGTAATGGTG ATAATTGGGG GCCAGGAGAA CCCCAACAAC TCTTCGGCCT 
     1261 CCTCTGAACC CCGGCCTGAG GGTCACAATA ATCCTCAGGT CATGGACACA GAGCATAGCA 
     1321 ACCCCCCAGA TTCTGGGTCC TCCGTGCCCA CGGATCCCAC CTGGGGCCCA GAGAGGAGAG 
     1381 GAGAGGAGTC TCCAGGGCAT TTCCTGGTCG CTGCCACAGG TACAGCGGCT GGGGGAGGCG 
     1441 GGGGCCTGAT CTTGGCCAGT CCCAAGCTGG GAGCAACCCC ATTACCTCCA GAATCGACCC 
     1501 CTGCACCCCC TCCTCCTCCA CCACCCCCTC CGCCCCCAGG GGTAGGCAGT GGCCACTTGA 
     1561 ATATCCCCCT GATCTTGGAA GAGCTACGGG TGCTGCAGCA GCGGCAGATC CATCAGATGC 
     1621 AGATGACTGA GCAAATCTGC AGGCAGGTGC TGTTGCTTGG CTCCTTAGGC CAGACGGTGG 
     1681 GTGCCCCTGC CAGTCCCTCA GAGCTACCTG GGACAGGGAC TGCCTCTTCC ACCAAGCCCC 
     1741 TACTACCCCT CTTCAGCCCC ATCAAGCCTG TCCAAACCAG CAAGACACTG GCATCTTCCT 
     1801 CCTCCTCCTC CTCTTCCTCT TCAGGGGCAG AAACGCCCAA GCAGGCCTTC TTCCACCTTT 
     1861 ACCACCCACT GGGGTCACAG CATCCTTTCT CTGCTGGAGG GGTTGGGCGA AGCCACAAAC 
     1921 CCACCCCTGC CCCTTCCCCA GCCTTGCCAG GCAGCACAGA TCAGCTGATT GCCTCGCCTC 
     1981 ATCTGGCATT CCCAAGCACC ACGGGACTAC TGGCAGCACA GTGTCTTGGG GCAGCCCGAG 
     2041 GCCTTGAGGC CACTGCCTCC CCAGGGCTCC TGAAGCCAAA GAATGGAAGT GGTGAGCTGA 
     2101 GCTACGGAGA AGTGATGGGT CCCTTGGAGA AGCCTGGTGG AAGGCACAAA TGCCGCTTCT 
     2161 GTGCCAAAGT ATTTGGCAGT GACAGTGCCC TGCAGATCCA CCTTCGTTCC CACACGGGTG 
     2221 AGAGGCCCTA TAAGTGCAAT GTCTGTGGAA ACCGTTTTAC CACCCGTGGC AACCTCAAAG 
     2281 TGCATTTCCA CCGGCATCGT GAGAAGTACC CACATGTGCA GATGAACCCA CACCCAGTAC 
     2341 CAGAGCACCT AGACTATGTC ATTACCAGCA GTGGCTTGCC TTATGGTATG TCCGTGCCAC 
     2401 CAGAGAAGGC CGAGGAGGAG GCAGCCACTC CAGGTGGAGG GGTTGAGCGC AAGCCTCTGG 
     2461 TGGCCTCCAC AACAGCACTC AGTGCCACAG AGAGCCTGAC TCTGCTCTCC ACCAGTGCAG 
     2521 GCACAGCCAC GGCTCCAGGA CTCCCTGCTT TCAATAAGTT TGTGCTCATG AAAGCAGTGG 
     2581 AACCCAAGAA TAAAGCTGAT GAAAACACCC CCCCAGGGAG TGAGGGCTCA GCCATCAGTG 
     2641 GAGTGGCAGA AAGTAGCACG GCAACTCGCA TGCAACTAAG TAAGTTGGTG ACTTCACTAC 
     2701 CAAGCTGGGC ACTGCTTACC AACCACTTCA AGTCCACTGG CAGCTTCCCC TTCCCCTATG 
     2761 TGCTAGAGCC CTTGGGGGCC TCACCCTCTG AGACATCAAA GCTGCAGCAA CTGGTAGAAA 
     2821 AGATTGACCG GCAAGGAGCT GTGGCGGTGA CCTCAGCTGC CTCAGGAGCC CCCACCACCT 
     2881 CTGCCCCTGC ACCTTCATCC TCAGCCTCTT CTGGACCTAA CCAGTGTGTC ATCTGTCTCC 
     2941 GAGTGCTTAG CTGTCCTCGG GCCCTACGCC TTCATTATGG CCAACATGGA GGTGAGAGGC 
     3001 CCTTCAAATG CAAAGTGTGT GGCAGAGCCT TCTCCACCAG GGGTAATCTG CGTGCACATT 
     3061 TCGTGGGCCA CAAGGCCAGT CCAGCTGCCC GGGCACAGAA TTCCTGCCCC ATCTGCCAGA 
     3121 AGAAGTTCAC CAATGCTGTC ACTCTGCAGC AGCATGTCCG GATGCACCTG GGGGGCCAGA 
     3181 TCCCCAACGG TGGTACTGCA CTCCCTGAAG GTGGAGGAGC TGCTCAGGAG AATGGCTCCG 
     3241 AGCAATCTAC AGTCTCCGGG GCAGGGAGTT TCCCCCAGCA GCAGTCCCAG CAGCCATCAC 
     3301 CGGAAGAGGA GTTGTCTGAG GAGGAGGAAG AGGAGGATGA GGAAGAAGAG GAAGATGTGA 
     3361 CTGATGAAGA TTCCCTGGCA GGGAGAGGCT CAGAGAGTGG AGGTGAGAAG GCAATATCAG 
     3421 TGAGAGGTGA TTCAGAAGAG GCATCTGGGG CAGAGGAGGA GGTGGGGACA GTGGCGGCAG 
     3481 CAGCCACAGC TGGGAAGGAG ATGGACAGTA ATGAGAAAAC TACTCAACAG TCTTCTTTGC 
     3541 CACCACCACC ACCACCTGAC AGCCTGGATC AGCCTCAGCC AATGGAGCAG GGAAGCAGTG 
     3601 GTGTTTTAGG AGGCAAGGAA GAGGGGGGCA AACCGGAGAG AAGCTCAAGT CCGGCATCAG 
     3661 CACTCACCCC AGAAGGGGAA GCCACCAGCG TGACCTTGGT AGAGGAGCTG AGCCTGCAGG 
     3721 AGGCAATGAG AAAGGAGCCA GGAGAGAGCA GCAGCAGAAA GGCCTGCGAA GTGTGTGGCC 
     3781 AGGCCTTTCC CTCCCAGGCA GCTCTGGAGG AGCATCAGAA GACCCACCCC AAGGAGGGGC 
     3841 CGCTCTTCAC TTGTGTTTTC TGCAGGCAGG GCTTTCTTGA GCGGGCTACC CTCAAGAAGC 
     3901 ATATGCTCCT GGCACACCAC CAGGTACAGC CCTTTGCCCC CCATGGCCCT CAGAATATTG 
     3961 CTGCTCTTTC TCTAGTCCCT GGCTGTTCGC CTTCCATCAC CTCCACAGGG CTCTCCCCCT 
     4021 TTCCCCGAAA AGATGACCCC ACGATCCCAA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4081 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4141 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4201 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4261 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4321 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4381 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4441 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4501 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4561 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4621 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4681 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4741 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4801 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4861 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4921 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4981 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5041 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5101 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5161 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5221 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5281 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5341 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5401 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5461 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5521 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5581 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5641 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5701 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5761 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5821 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5881 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5941 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6001 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6061 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6121 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6181 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6241 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6301 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6361 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6421 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6481 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6541 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6601 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6661 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6721 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6781 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6841 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6901 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6961 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7021 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7081 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7141 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7201 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7261 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7321 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7381 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7441 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7501 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7561 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7621 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7681 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7741 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7801 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7861 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//