LOCUS       RC218432                9204 bp    DNA     circular  SYN 
DEFINITION  RSF1 (Myc-DDK-tagged)-Human remodeling and spacing factor 1 (RSF1)
ACCESSION   NM_016578
VERSION     NM_016578.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5361..5452 
                     /label="Myc-DDK"  
     misc_feature    5482..6067 
                     /label="PolyA signal"  
     misc_feature    5539..5558 
                     /label="Restriction Enzyme"  
     misc_feature    6343..7015 
                     /label="ColE1"  
     misc_feature    7445..8239 
                     /label="Kan/Neo"  
     misc_feature    8274..8576 
                     /label="SV40 ori"  
     misc_feature    8662..9139 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5343..5349 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5343 
                     /label="RSF1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACGGCG GCGGCAGCGG CGGCGGTGAT GGCTCCTCCG GGCTGCCCGG 
     1081 GTTCGTGCCC CAACTTCGCC GTAGTCTGCT CCTTCTTGGA GCGCTACGGG CCGCTGCTAG 
     1141 ACCTGCCTGA GTTGCCGTTC CCTGAGCTGG AGCGGGTGCT GCAGGCGCCG CCGCCGGACG 
     1201 TCGGCAACGG AGAAGTACCA AAAGAATTGG TGGAGCTCCA TTTGAAGCTG ATGAGGAAAA 
     1261 TTGGCAAATC TGTTACTGCA GACAGATGGG AAAAATATTT GATCAAGATA TGCCAAGAGT 
     1321 TTAACAGTAC CTGGGCATGG GAGATGGAGA AGAAGGGCTA TCTTGAAATG AGTGTTGAAT 
     1381 GCAAACTAGC ACTCTTAAAG TACCTCTGTG AGTGTCAGTT TGATGACAAT CTCAAATTCA 
     1441 AGAATATTAT TAATGAGGAG GATGCCGATA CTATGCGTCT CCAGCCAATT GGTCGAGACA 
     1501 AAGATGGCCT CATGTACTGG TACCAATTGG ATCAAGATCA CAATGTCAGA ATGTACATAG 
     1561 AAGAACAAGA TGATCAAGAT GGCTCTTCAT GGAAATGCAT TGTCAGAAAT CGAAACGAGT 
     1621 TGGCTGAGAC TCTTGCACTC CTGAAAGCAC AAATTGATCC TGTACTATTG AAAAACTCTA 
     1681 GCCAACAAGA CAACTCTTCT CGGGAAAGTC CCAGCTTAGA GGATGAGGAG ACTAAAAAAG 
     1741 AGGAAGAAAC ACCTAAACAA GAGGAACAGA AAGAAAGTGA AAAGATGAAA AGTGAGGAGC 
     1801 AGCCTATGGA TTTAGAAAAC CGTTCTACAG CCAATGTTCT AGAAGAGACT ACTGTGAAAA 
     1861 AAGAAAAAGA AGATGAAAAG GAACTTGTGA AACTGCCAGT CATAGTGAAG CTAGAAAAAC 
     1921 CTTTGCCAGA AAATGAAGAA AAAAAGATTA TCAAAGAAGA AAGTGATTCC TTCAAGGAAA 
     1981 ATGTCAAACC CATTAAAGTT GAGGTGAAGG AATGTAGAGC AGATCCTAAA GATACCAAAA 
     2041 GTAGCATGGA GAAGCCAGTG GCACAGGAGC CTGAAAGGAT CGAATTTGGT GGCAATATTA 
     2101 AATCTTCTCA CGAAATTACT GAGAAATCTA CTGAAGAAAC TGAGAAACTT AAAAATGACC 
     2161 AGCAGGCCAA GATACCACTA AAAAAACGAG AAATTAAACT GAGTGATGAT TTTGACAGTC 
     2221 CAGTCAAGGG ACCTTTGTGT AAATCAGTTA CTCCAACAAA AGAGTTTTTG AAAGATGAAA 
     2281 TAAAACAAGA GGAAGAGACT TGTAAAAGGA TCTCTACAAT CACTGCTTTG GGTCATGAAG 
     2341 GGAAACAGCT GGTAAATGGA GAAGTTAGTG ATGAAAGGGT AGCTCCAAAT TTTAAGACAG 
     2401 AACCAATAGA GACAAAGTTT TATGAGACAA AGGAAGAGAG CTATAGCCCC CCTAAGGACA 
     2461 GAAATATCAT CACGGAGGGA AATGGAACAG AGTCCTTAAA TTCTGTCATA ACAAGTATGA 
     2521 AAACAGGTGA GCTTGAGAAA GAAACAGCCC CTTTGAGGAA AGATGCAGAT AGTTCAATAT 
     2581 CAGTCTTAGA GATCCATAGT CAAAAAGCAC AAATAGAGGA ACCCGATCCT CCAGAAATGG 
     2641 AAACTTCTCT TGATTCTTCT GAGATGGCAA AAGATCTCTC TTCAAAAACT GCTTTATCTT 
     2701 CCACCGAGTC GTGTACCATG AAAGGTGAAG AGAAGTCTCC CAAAACTAAG AAGGATAAGC 
     2761 GCCCACCAAT CCTAGAATGT CTTGAAAAGT TAGAGAAGTC CAAAAAGACT TTTCTTGATA 
     2821 AGGACGCACA AAGATTGAGT CCAATACCAG AAGAAGTTCC AAAGAGTACT CTAGAGTCAG 
     2881 AAAAGCCTGG CTCTCCTGAG GCAGCTGAAA CTTCTCCACC ATCTAATATC ATTGACCACT 
     2941 GTGAGAAACT AGCCTCAGAA AAAGAAGTGG TAGAATGCCA GAGTACAAGT ACTGTTGGTG 
     3001 GCCAGTCTGT GAAAAAAGTA GACCTAGAAA CCCTAAAAGA GGATTCTGAG TTCACAAAGG 
     3061 TAGAAATGGA TAATCTGGAC AATGCCCAGA CCTCTGGCAT AGAGGAGCCT TCTGAGACAA 
     3121 AGGGTTCTAT GCAAAAAAGC AAATTCAAAT ATAAGTTGGT TCCTGAAGAA GAAACCACTG 
     3181 CCTCAGAAAA TACAGAGATA ACCTCTGAAA GGCAGAAAGA GGGCATCAAA TTAACAATCA 
     3241 GGATATCAAG TCGGAAAAAG AAGCCCGATT CTCCCCCCAA AGTTCTAGAA CCAGAAAACA 
     3301 AGCAAGAGAA GACAGAAAAG GAAGAGGAGA AAACAAATGT GGGTCGTACT TTAAGAAGAT 
     3361 CTCCAAGAAT ATCTAGACCC ACTGCAAAAG TGGCTGAGAT CAGAGATCAG AAAGCTGATA 
     3421 AAAAAAGAGG GGAAGGAGAA GATGAGGTGG AAGAAGAGTC AACAGCTTTG CAAAAAACTG 
     3481 ACAAAAAGGA AATTTTGAAA AAATCAGAGA AAGATACAAA TTCTAAAGTA AGCAAGGTAA 
     3541 AACCCAAAGG CAAAGTTCGA TGGACTGGTT CTCGGACACG TGGCAGATGG AAATATTCCA 
     3601 GCAATGATGA AAGTGAAGGG TCTGGCAGTG AAAAATCATC TGCAGCTTCA GAAGAGGAGG 
     3661 AAGAAAAGGA AAGTGAAGAA GCCATCCTAG CAGATGATGA TGAACCATGC AAAAAATGTG 
     3721 GCCTTCCAAA CCATCCTGAG CTAATTCTTC TGTGTGACTC TTGCGATAGT GGATACCATA 
     3781 CTGCCTGCCT TCGCCCTCCT CTGATGATCA TCCCAGATGG AGAATGGTTC TGCCCACCTT 
     3841 GCCAACATAA ACTGCTCTGT GAAAAATTAG AGGAACAGTT GCAGGATTTG GATGTTGCCT 
     3901 TAAAGAAGAA AGAGCGTGCC GAACGAAGAA AAGAACGCTT GGTGTATGTT GGTATCAGTA 
     3961 TTGAAAACAT CATTCCTCCA CAAGAGCCAG ACTTTTCTGA AGATCAAGAA GAAAAGAAAA 
     4021 AAGATTCAAA AAAATCCAAA GCAAACTTGC TTGAAAGGAG GTCAACAAGA ACAAGGAAAT 
     4081 GTATAAGCTA CAGATTTGAT GAGTTTGATG AAGCAATTGA TGAAGCTATT GAAGATGACA 
     4141 TCAAAGAAGC CGATGGAGGA GGAGTTGGCC GAGGAAAAGA TATCTCCACC ATCACAGGTC 
     4201 ATCGTGGGAA AGACATCTCT ACTATTTTGG ATGAAGAAAG AAAAGAAAAT AAACGACCCC 
     4261 AGAGGGCAGC TGCTGCTCGA AGGAAGAAAC GCCGGCGATT AAATGATCTG GACAGTGATA 
     4321 GCAACCTGGA TGAAGAAGAG AGCGAGGATG AATTCAAGAT CAGTGATGGA TCTCAAGATG 
     4381 AGTTTGTTGT GTCTGATGAA AACCCAGATG AAAGTGAAGA AGATCCGCCA TCTAATGATG 
     4441 ACAGTGACAC TGACTTTTGT AGCCGTAGAC TGAGGCGACA CCCCTCTCGG CCAATGAGGC 
     4501 AGAGCAGGCG TTTGCGAAGA AAGACCCCAA AGAAAAAATA TTCCGATGAT GATGAAGAGG 
     4561 AGGAATCTGA GGAGAATAGT AGAGACTCTG AAAGTGACTT CAGTGATGAT TTTAGTGATG 
     4621 ATTTTGTAGA AACTCGGCGA AGGCGGTCAA GGAGAAATCA GAAAAGACAA ATTAACTACA 
     4681 AAGAAGACTC AGAAAGTGAC GGTTCCCAGA AGAGTTTGCG ACGTGGTAAA GAAATAAGGC 
     4741 GAGTACACAA GCGAAGACTT TCCAGCTCAG AGAGTGAAGA GAGCTATTTG TCCAAGAACT 
     4801 CTGAAGATGA TGAGCTAGCT AAAGAATCAA AGCGGTCAGT TCGAAAGCGG GGCCGAAGCA 
     4861 CAGACGAGTA TTCAGAAGCA GATGAGGAGG AGGAGGAAGA GGAAGGCAAA CCATCCCGCA 
     4921 AACGGCTACA CCGGATTGAG ACGGATGAGG AGGAGAGTTG TGACAATGCT CATGGAGATG 
     4981 CAAATCAGCC TGCCCGTGAC AGCCAGCCTA GGGTCCTGCC CTCAGAACAA GAGAGCACCA 
     5041 AGAAGCCCTA CCGGATAGAA AGTGATGAGG AAGAGGACTT TGAAAATGTA GGCAAAGTGG 
     5101 GGAGCCCATT GGACTATAGC TTAGTGGACT TACCTTCAAC CAATGGACAG AGCCCTGGCA 
     5161 AAGCCATTGA GAACTTGATT GGCAAGCCTA CTGAGAAGTC TCAGACCCCC AAGGACAACA 
     5221 GCACAGCCAG TGCAAGCCTA GCCTCCAATG GGACAAGTGG TGGGCAGGAG GCAGGAGCAC 
     5281 CAGAAGAGGA GGAAGATGAG CTTTTGAGAG TGACTGACCT TGTTGATTAT GTCTGTAACA 
     5341 GTGAACAGTT AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     5401 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     5461 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     5521 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5581 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5641 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5701 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5761 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5821 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5881 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5941 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     6001 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     6061 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     6121 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     6181 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     6241 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     6301 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     6361 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     6421 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     6481 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6541 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6601 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6661 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6721 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6781 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6841 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6901 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6961 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     7021 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     7081 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     7141 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     7201 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     7261 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     7321 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     7381 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     7441 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     7501 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7561 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7621 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7681 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7741 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7801 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7861 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7921 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7981 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     8041 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     8101 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     8161 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     8221 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     8281 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     8341 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     8401 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     8461 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8521 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8581 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8641 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8701 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8761 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8821 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8881 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8941 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     9001 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     9061 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     9121 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     9181 TAACAAAAAT TTAACGCGAA TTTT
//