LOCUS       RC218422                7806 bp    DNA     circular  SYN 
DEFINITION  RNF20 (Myc-DDK-tagged)-Human ring finger protein 20 (RNF20)
ACCESSION   NM_019592
VERSION     NM_019592.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3963..4054 
                     /label="Myc-DDK"  
     misc_feature    4084..4669 
                     /label="PolyA signal"  
     misc_feature    4141..4160 
                     /label="Restriction Enzyme"  
     misc_feature    4945..5617 
                     /label="ColE1"  
     misc_feature    6047..6841 
                     /label="Kan/Neo"  
     misc_feature    6876..7178 
                     /label="SV40 ori"  
     misc_feature    7264..7741 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3945..3951 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3945 
                     /label="RNF20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGGAATT GGAAATAAAA GAGCAGCTGG AGAACCTGGC ACCTCCATGC 
     1081 CTCCTGAGAA GAAGGCAGCT GTTGAAGATT CAGGGACCAC AGTGGAAACA ATTAAGCTAG 
     1141 GAGGTGTCTC TTCAACGGAG GAACTAGACA TTAGAACACT GCAAACCAAA AATCGCAAGC 
     1201 TGGCAGAAAT GTTGGATCAG CGGCAGGCCA TTGAAGATGA ACTTCGTGAG CACATTGAAA 
     1261 AACTGGAACG ACGACAGGCC ACTGATGATG CCTCACTATT GATTGTCAAC CGATACTGGA 
     1321 GTCAGTTTGA TGAAAACATC CGTATCATCC TTAAACGTTA TGATCTGGAG CAGGGCTTGG 
     1381 GAGACCTACT CACAGAACGA AAAGCCCTTG TTGTGCCTGA ACCAGAACCA GACTCTGATA 
     1441 GCAATCAGGA GCGTAAAGAT GACCGAGAGA GAGGGGAAGG GCAAGAGCCA GCTTTCTCTT 
     1501 TCCTTGCTAC TTTGGCCAGC AGTTCCAGTG AAGAGATGGA GTCTCAGCTG CAGGAACGTG 
     1561 TGGAGTCTTC CCGCCGAGCC GTGTCCCAGA TTGTGACTGT TTATGATAAA TTGCAAGAAA 
     1621 AAGTGGAGCT CTTATCCCGG AAGCTAAACA GTGGAGATAA TCTGATAGTG GAGGAAGCAG 
     1681 TGCAGGAGCT GAACTCTTTC CTCGCACAGG AGAATATGAG GCTACAGGAA TTGACAGATC 
     1741 TTCTTCAGGA AAAGCATCGC ACCATGTCTC AGGAGTTCTC CAAGTTGCAG AGTAAAGTGG 
     1801 AGACAGCCGA ATCACGAGTG TCTGTCCTGG AGTCCATGAT TGATGACCTG CAGTGGGATA 
     1861 TTGACAAAAT TCGAAAGAGG GAACAGCGAC TCAACCGACA CTTAGCAGAA GTCCTAGAAC 
     1921 GGGTGAATTC CAAAGGTTAT AAGGTGTATG GAGCGGGGAG CAGTCTGTAT GGCGGCACAA 
     1981 TCACTATCAA TGCTCGGAAG TTTGAGGAAA TGAATGCAGA GCTTGAGGAG AACAAAGAGT 
     2041 TGGCTCAGAA CCGTCTCTGT GAGCTGGAGA AACTTCGGCA AGACTTTGAG GAGGTCACTA 
     2101 CACAAAATGA AAAGCTGAAG GTGGAATTGC GGAGTGCAGT GGAGCAAGTC GTTAAGGAAA 
     2161 CTCCAGAATA TCGCTGCATG CAGTCACAGT TCTCCGTCTT GTATAATGAG AGCCTACAGT 
     2221 TGAAAGCACA CTTGGATGAG GCTCGGACCC TGCTTCATGG CACCAGAGGA ACCCACCAGC 
     2281 ACCAGGTTGA GCTTATTGAG CGAGATGAGG TTAGTCTTCA TAAGAAGCTG AGGACTGAAG 
     2341 TAATTCAGCT AGAAGATACA TTGGCCCAGG TCCGCAAGGA GTATGAAATG CTGAGGATAG 
     2401 AATTTGAGCA GACCCTTGCT GCCAATGAAC AAGCAGGCCC TATAAACAGG GAGATGCGCC 
     2461 ACCTCATCAG TAGCCTCCAG AATCACAATC ACCAGCTGAA AGGGGAGGTC CTGAGATATA 
     2521 AGCGGAAATT GAGAGAAGCC CAGTCTGACC TGAACAAGAC ACGCCTGCGT AGTGGTAGTG 
     2581 CCCTCCTGCA GTCCCAGTCT AGTACTGAGG ACCCGAAGGA TGAGCCTGCG GAGCTAAAAC 
     2641 CAGATTCTGA GGACTTATCC TCCCAGTCCT CAGCTTCAAA GGCATCTCAG GAGGATGCCA 
     2701 ATGAAATCAA GTCTAAACGG GATGAAGAAG AACGAGAACG AGAAAGGAGG GAGAAGGAGA 
     2761 GGGAACGAGA AAGAGAACGG GAGAAGGAGA AGGAGAGAGA ACGAGAGAAG CAGAAGCTAA 
     2821 AAGAGTCAGA AAAAGAGAGA GATTCTGCTA AGGATAAAGA GAAAGGCAAA CATGATGATG 
     2881 GACGGAAAAA GGAAGCAGAA ATTATCAAAC AATTGAAGAT TGAACTCAAG AAGGCACAGG 
     2941 AGAGCCAAAA GGAGATGAAA CTATTGCTGG ATATGTACCG TTCTGCCCCA AAGGAACAGA 
     3001 GAGACAAAGT TCAGCTGATG GCAGCTGAGA AGAAGTCTAA GGCAGAGTTG GAAGATCTAA 
     3061 GGCAAAGACT CAAGGATCTG GAAGATAAAG AGAAGAAAGA GAACAAGAAA ATGGCTGATG 
     3121 AGGATGCCTT GAGGAAGATC CGGGCAGTGG AGGAGCAGAT AGAATACCTA CAGAAGAAGC 
     3181 TAGCCATGGC CAAGCAGGAA GAAGAAGCAC TCCTCTCTGA AATGGATGTC ACAGGCCAGG 
     3241 CCTTTGAAGA CATGCAGGAG CAAAATATCC GTTTGATGCA GCAATTGCGG GAGAAGGATG 
     3301 ATGCAAATTT CAAGCTCATG TCAGAGCGTA TCAAGTCCAA TCAGATCCAT AAGTTGCTTA 
     3361 AAGAAGAGAA GGAGGAGCTG GCAGACCAGG TGTTGACTCT GAAGACTCAG GTTGATGCCC 
     3421 AGCTACAGGT AGTAAGGAAA CTGGAAGAGA AGGAGCATCT GTTACAGAGC AACATTGGCA 
     3481 CAGGGGAGAA AGAGCTGGGT CTTAGGACCC AAGCCTTAGA GATGAATAAA CGCAAGGCAA 
     3541 TGGAGGCAGC CCAGCTTGCA GATGACCTCA AAGCACAACT GGAGTTGGCT CAGAAGAAGC 
     3601 TACATGATTT TCAGGATGAG ATCGTGGAGA ACAGTGTTAC CAAAGAAAAG GACATGTTCA 
     3661 ATTTCAAACG AGCCCAGGAG GACATCTCTA GACTTCGCAG GAAGCTGGAG ACCACAAAGA 
     3721 AACCAGACAA TGTACCCAAG TGTGATGAGA TTCTGATGGA AGAGATTAAG GATTACAAGG 
     3781 CACGCTTGAC CTGTCCGTGC TGTAACATGC GTAAAAAGGA TGCTGTTCTT ACTAAGTGTT 
     3841 TTCATGTCTT CTGCTTTGAG TGTGTGAAGA CACGCTATGA CACCCGCCAG CGCAAATGTC 
     3901 CCAAGTGTAA TGCTGCTTTT GGTGCCAATG ATTTTCATCG CATCTACATT GGTACGCGTA 
     3961 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4021 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4081 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4141 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4201 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4261 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4321 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4381 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4441 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4501 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4561 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4621 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4681 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4741 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4801 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4861 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4921 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4981 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5041 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5101 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5161 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5221 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5281 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5341 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5401 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5461 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5521 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5581 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5641 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5701 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5761 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5821 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5881 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5941 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6001 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6061 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6121 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6181 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6241 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6301 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6361 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6421 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6481 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6541 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6601 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6661 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6721 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6781 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6841 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6901 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6961 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7021 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7081 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7141 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7201 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7261 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7321 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7381 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7441 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7501 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7561 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7621 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7681 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7741 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7801 AATTTT
//