LOCUS RC218385 8652 bp DNA circular SYN DEFINITION IRS4 (Myc-DDK-tagged)-Human insulin receptor substrate 4 (IRS4) ACCESSION NM_003604 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 4809..4900 /label="Myc-DDK" misc_feature 4930..5515 /label="PolyA signal" misc_feature 4987..5006 /label="Restriction Enzyme" misc_feature 5791..6463 /label="ColE1" misc_feature 6893..7687 /label="Kan/Neo" misc_feature 7722..8024 /label="SV40 ori" misc_feature 8110..8587 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 4791..4797 /label="Restriction Enzyme" misc_feature 1020..4791 /label="IRS4" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCGAGTTGC TCCTTCACTC GCGACCAAGC GACAAGAAGA CTAAGAGGTG 1081 CAGCAGCGGC GGCAGCGGCA GCTCTAGCAG CAGTGGTGAC CACCCCGCTT CTTTCCTCGG 1141 GAACCCCGAC CGCACTCATT GGGACCGGGT CGTCTTGTCC GGGAGCCATG TGGCTCTCCA 1201 CGGCCACTGG CTCCCGGTCA GACTCCGAGT CCGAAGAGGA GGACCTGCCC GTCGGGGAGG 1261 AAGTCTGCAA ACGCGGCTAC CTGCGGAAAC AGAAGCATGG GCACAGGCGC TACTTCGTGC 1321 TCAAACTCGA GACTGCTGAC GCCCCAGCTC GGCTGGAATA CTACGAAAAT GCCAGGAAGT 1381 TCCGGCACAG TGTCCGCGCC GCGGCGGCTG CAGCAGCGGC GGCCGCCTCT GGCGCCGCGA 1441 TCCCCCCGCT CATTCCACCG CGGCGCGTGA TCACCCTATA CCAGTGCTTT TCCGTGAGCC 1501 AGCGAGCAGA TGCAAGGTAC CGACACCTCA TTGCTCTTTT CACCCAAGAC GAATACTTCG 1561 CGATGGTGGC CGAGAACGAG TCGGAGCAGG AAAGCTGGTA CTTGCTGCTC AGCCGCCTCA 1621 TCCTCGAGAG CAAGCGCCGC CGCTGCGGCA CGCTCGGCGC GCAGCCGGAC GGAGAGCCGG 1681 CCGCGCTGGC GGCGGCAGCG GCGGCGGAGC CACCCTTCTA TAAAGATGTG TGGCAGGTAA 1741 TAGTCAAACC CAGGGGGCTG GGGCACAGAA AAGAGCTGAG CGGCGTGTTC CGGCTGTGTC 1801 TAACCGACGA GGAGGTCGTG TTTGTGAGGC TGAACACCGA AGTGGCCAGC GTGGTCGTCC 1861 AGCTCCTGAG CATCCGTCGC TGTGGACACT CGGAGCAGTA TTTCTTCTTG GAAGTAGGCA 1921 GGTCCACTGT CATCGGTCCG GGAGAGCTCT GGATGCAGGT CGATGACTGT GTGGTTGCCC 1981 AAAACATGCA TGAGCTGTTT TTGGAGAAGA TGAGAGCCTT GTGTGCAGAC GAATACAGAG 2041 CCCGCTGCCG CAGCTACAGC ATCAGCATCG GCGCCCACCT GTTAACCCTG CTGTCCGCTA 2101 GGAGGCACCT GGGCTTGGTG CCGCTCGAGC CGGGAGGCTG GCTCAGAAGG TCCCGCTTTG 2161 AGCAGTTTTG CCACCTCAGG GCCATCGGCG ACGGGGAAGA CGAGATGCTT TTCACCAGGC 2221 GCTTCGTAAC ACCCAGCGAG CCTGTGGCCC ACTCCAGGCG AGGAAGACTG CACCTGCCCA 2281 GAGGGCGCAG GTCAAGGAGA GCGGTTTCAG TGCCGGCCAG CTTTTTTCGC CGCTTAGCAC 2341 CCAGCCCAGC ACGTCCCCGG CACCCTGCAG AAGCCCCGAA CAATGGAGCT CGCCTGTCTT 2401 CTGAAGTGTC TGGTTCTGGC TCTGGCAACT TTGGGGAGGA AGGCAATCCC CAGGGCAAAG 2461 AAGATCAGGA AGGAAGCGGA GGTGACTACA TGCCTATGAA CAATTGGGGC TCAGGAAATG 2521 GCCGGGGCTC AGGAGGTGGC CAGGGCTCAA ATGGCCAAGG CTCCAGTAGC CATAGCTCGG 2581 GAGGAAACCA GTGTTCAGGC GAGGGACAGG GATCCCGAGG TGGTCAGGGC TCAAATGGCC 2641 AGGGCTCAGG AGGAAACCAG TGCTCTAGAG ATGGCCAGGG CACCGCAGGT GGGCACGGTT 2701 CAGGTGGTGG CCAGAGACCT GGAGGTGGGC ATGGCTCAGG TGGTGGCCAG GGACCTGGAG 2761 ATGGCCATGG CTCAGGTGGT GGCAAGAACT CTGGGGGGGG CAAAGGCTCA GGAAGTGGGA 2821 AAGGATCCGA TGGTGATGGT GAACGTGGAA AATCTCTGAA GAAAAGATCC TATTTTGGCA 2881 AATTAACTCA AAGCAAGCAA CAGCAAATGC CACCACCTCC ACCACCTCCT CCTCCACCCC 2941 CACCAGCTGG AGGAACTGGT GGAAAAGGGA AGTCTGGGGG AAGATTCAGA CTTTATTTTT 3001 GTGTTGACAG AGGAGCCACG AAAGAATGCA AAGAAGCCAA AGAAGTGAAA GATGCAGAGA 3061 TCCCAGAAGG TGCAGCTCGA GGTCCCCACA GAGCCAGAGC TTTTGATGAA GATGAGGATG 3121 ACCCATACGT GCCAATGAGG CCAGGGGTGG CCACCCCTCT TGTAAGCTCC AGTGATTATA 3181 TGCCAATGGC TCCTCAAAAT GTCTCTGCTT CAAAAAAGCG CCACTCTCGA TCCCCTTTTG 3241 AAGATTCAAG AGGGTACATG ATGATGTTTC CCAGAGTGAG CCCACCACCT GCTCCGAGTC 3301 CTCCAAAAGC ACCTGATACT AATAAAGAGG ATGACTCAAA GGACAATGAC AGTGAGAGTG 3361 ACTACATGTT TATGGCTCCT GGAGCCGGTG CAATTCCAAA AAACCCCAGA AATCCTCAGG 3421 GTGGCTCTTC CTCCAAAAGT TGGAGCTCCT ACTTCTCTCT ACCAAACCCT TTTCGGAGCT 3481 CACCTTTGGG ACAGAATGAC AACAGTGAGT ATGTGCCAAT GTTACCTGGA AAGTTCCTGG 3541 GGAGGGGCCT AGACAAAGAA GTCTCCTATA ACTGGGACCC CAAAGATGCA GCTTCAAAGC 3601 CTTCAGGTGA GGGATCATTC TCAAAGCCTG GAGATGGGGG ATCACCTTCA AAGCCTTCAG 3661 ATGATGAGCC CCCAAAGAAT AAAGCTAAGA GACCTAACCG ACTTTCTTTT ATTACAAAAG 3721 GATATAAAAT CAAGCCAAAA CCACAAAAGC CCACACATGA GCAGAGAGAA GCTGACAGCT 3781 CTAGTGACTA CGTCAACATG GACTTCACTA AAAGAGAGAG CAATACACCA GCTCCCTCTA 3841 CTCAAGGACT ACCAGATTCG TGGGGCATAA TTGCTGAACC CAGACAGTCA GCCTTTTCTA 3901 ATTATGTGAA TGTTGAGTTT GGAGTGCCAT TTCCAAATCC AGCAAACGAC CTCTCAGATC 3961 TTTTAAGAGC TATACCACGT GCCAACCCCT TATCTCTGGA CAGTGCTAGG TGGCCACTTC 4021 CTCCCCTTCC CCTCAGTGCT ACAGGTAGCA ATGCTATTGA GGAAGAGGGT GACTACATTG 4081 AAGTAATTTT CAACTCAGCA ATGACACCAG CCATGGCTCT TGCTGACAGT GCCATTCGCT 4141 ATGATGCTGA AACAGGTCGA ATCTATGTGG TCGACCCATT TTCTGAGTGC TGTATGGATA 4201 TTTCTCTCTC CCCCAGCCGA TGTTCTGAAC CACCACCTGT AGCTAGGCTG CTGCAGGAAG 4261 AAGAGCAGGA GAGAAGACGC CCACAAAGCC GTTCTCAAAG TTTCTTTGCA GCAGCCAGAG 4321 CCGCTGTCTC TGCTTTTCCA ACAGACAGCC TCGAGAGAGA CCTTTCCCCA TCCTCAGCCC 4381 CGGCTGTCGC TTCGGCTGCA GAGCCGACTT TAGCCCTCAG CCAAGTTGTA GCTGCGGCCT 4441 CCGCGCTCGC CGCAGCCCCG GGCATCGGCG CAGCAGCCGC AGCTGCTGGA TTTGACTCCG 4501 CCTCTGCCCG CTGGTTTCAA CCTGTTGCTA ATGCTGCTGA TGCCGAAGCA GTAAGGGGAG 4561 CCCAAGACGT TGCCGGTGGC TCGAACCCTG GAGCCCACAA CCCATCTGCA AACCTTGCCA 4621 GAGGTGATAA CCAGGCTGGC GGGGCTGCCG CTGCAGCTGC CGCTCCGGAA CCACCACCTC 4681 GCAGTCGCCG GGTGCCAAGA CCCCCGGAGA GAGAAGATTC TGACAACGAC GACGACACTC 4741 ACGTGAGAAT GGATTTTGCC AGACGTGATA ATCAGTTCGA CTCTCCCAAA AGAGGTCGGA 4801 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 4861 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 4921 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 4981 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 5041 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 5101 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 5161 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 5221 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 5281 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 5341 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 5401 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 5461 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 5521 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 5581 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 5641 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 5701 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 5761 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 5821 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 5881 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 5941 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 6001 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 6061 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 6121 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 6181 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 6241 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 6301 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 6361 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 6421 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 6481 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 6541 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 6601 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 6661 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 6721 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 6781 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 6841 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 6901 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 6961 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 7021 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 7081 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 7141 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 7201 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 7261 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 7321 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 7381 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 7441 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 7501 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 7561 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 7621 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 7681 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 7741 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 7801 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 7861 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 7921 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 7981 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 8041 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 8101 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 8161 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 8221 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 8281 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 8341 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 8401 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 8461 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 8521 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 8581 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 8641 AACGCGAATT TT //