LOCUS       RC218299                9246 bp    DNA     circular  SYN 
DEFINITION  NFAT5 (Myc-DDK-tagged)-Human nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant 4
ACCESSION   NM_173214
VERSION     NM_173214.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5403..5494 
                     /label="Myc-DDK"  
     misc_feature    5524..6109 
                     /label="PolyA signal"  
     misc_feature    5581..5600 
                     /label="Restriction Enzyme"  
     misc_feature    6385..7057 
                     /label="ColE1"  
     misc_feature    7487..8281 
                     /label="Kan/Neo"  
     misc_feature    8316..8618 
                     /label="SV40 ori"  
     misc_feature    8704..9181 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5385..5391 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5385 
                     /label="NFAT5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGGTGCT TGCAGCTCCT TTACCACCTC TTCCAGCCCT ACCATTTATT 
     1081 CTACCTCAGT CACCGACAGC AAGGCTATGC AAGTGGAGAG CTGCTCCTCA GCCGTGGGGG 
     1141 TAAGTAACAG AGGGGTAAGT GAAAAGCAGT TAACCAGTAA CACAGTTCAG CAGCATCCAT 
     1201 CAACACCGAA GAGGCACACA GTCTTGTACA TCTCACCACC ACCTGAGGAC TTGCTGGATA 
     1261 ACAGTCGGAT GTCCTGCCAG GATGAGGGGT GTGGATTGGA ATCTGAGCAG AGCTGCAGTA 
     1321 TGTGGATGGA GGATTCCCCC TCCAACTTCA GTAACATGAG CACCAGTTCC TACAATGATA 
     1381 ACACTGAGGT ACCTCGTAAA TCACGAAAAC GAAATCCAAA GCAGAGGCCG GGGGTCAAAC 
     1441 GACGAGATTG TGAAGAATCT AATATGGATA TATTTGATGC CGACAGTGCC AAAGCACCTC 
     1501 ACTATGTGCT TTCTCAGCTT ACCACGGACA ACAAAGGCAA CTCAAAAGCG GGAAATGGAA 
     1561 CATTGGAAAA CCAAAAAGGA ACTGGAGTAA AGAAGAGCCC TATGTTGTGT GGACAATATC 
     1621 CTGTTAAAAG TGAGGGAAAG GAGCTGAAGA TAGTTGTACA ACCTGAGACA CAGCACCGAG 
     1681 CTCGGTACCT GACTGAGGGC AGCCGTGGCT CAGTGAAAGA TAGAACACAG CAAGGCTTTC 
     1741 CTACAGTAAA GCTGGAAGGC CATAATGAAC CTGTAGTGTT GCAAGTGTTT GTGGGCAACG 
     1801 ACTCTGGACG AGTGAAACCA CATGGATTTT ATCAGGCCTG CAGAGTAACT GGACGAAATA 
     1861 CAACTCCTTG CAAAGAAGTG GACATTGAAG GCACTACTGT TATAGAAGTC GGCCTTGATC 
     1921 CTAGCAACAA CATGACACTG GCGGTGGACT GCGTAGGGAT ATTGAAATTG AGGAATGCTG 
     1981 ATGTCGAAGC CAGAATAGGA ATTGCTGGTT CCAAGAAGAA AAGCACTCGT GCCAGATTGG 
     2041 TTTTTCGAGT TAATATCATG AGGAAAGATG GCTCCACTTT GACACTGCAA ACACCCTCTT 
     2101 CTCCAATTTT GTGTACTCAG CCAGCAGGAG TGCCAGAAAT CTTAAAGAAA AGCTTGCATA 
     2161 GCTGTTCAGT GAAAGGAGAA GAAGAAGTGT TTTTAATCGG CAAGAACTTT CTGAAAGGAA 
     2221 CTAAAGTTAT TTTCCAAGAA AATGTTTCTG ATGAAAACTC TTGGAAGTCA GAAGCTGAAA 
     2281 TTGATATGGA ACTATTTCAT CAGAATCATC TTATTGTGAA GGTTCCTCCC TATCATGACC 
     2341 AACATATAAC TTTGCCTGTG TCAGTGGGAA TATATGTAGT GACAAATGCT GGAAGATCTC 
     2401 ATGATGTTCA ACCATTCACT TACACTCCAG ACCCAGCAGC AGCTGGTGCT TTGAATGTAA 
     2461 ATGTGAAGAA GGAAATATCT AGTCCAGCAA GACCTTGCTC TTTTGAAGAG GCCATGAAAG 
     2521 CAATGAAAAC TACTGGATGT AATTTAGATA AGGTAAATAT TATCCCTAAT GCCCTGATGA 
     2581 CTCCACTCAT ACCAAGCAGT ATGATTAAGA GTGAAGATGT TACTCCAATG GAAGTAACAG 
     2641 CAGAAAAAAG ATCTTCCACT ATTTTTAAGA CTACAAAGTC TGTTGGATCA ACTCAGCAAA 
     2701 CATTAGAAAA CATCTCAAAC ATAGCAGGAA ATGGCTCTTT TTCATCACCA TCATCTTCCC 
     2761 ACCTACCTTC TGAAAATGAA AAACAGCAGC AGATTCAGCC CAAGGCATAC AACCCAGAGA 
     2821 CCCTGACAAC TATTCAAACC CAGGACATCT CACAGCCTGG TACTTTTCCA GCAGTTTCTG 
     2881 CTTCTAGTCA GCTGCCCAAC AGCGATGCAC TATTGCAGCA GGCTACACAG TTTCAGACAA 
     2941 GAGAAACTCA GTCTAGAGAG ATATTACAGT CAGATGGTAC AGTGGTTAAT TTGTCACAAC 
     3001 TGACTGAGGC ATCACAACAA CAGCAGCAGT CACCACTACA AGAACAAGCA CAGACTTTAC 
     3061 AGCAGCAGAT TTCATCAAAT ATTTTTCCAT CACCAAATAG TGTGAGTCAG CTTCAGAATA 
     3121 CTATTCAGCA GCTGCAAGCA GGGAGTTTCA CAGGCAGTAC TGCTAGTGGC AGCAGTGGAA 
     3181 GTGTTGACTT GGTCCAACAA GTTTTAGAGG CACAGCAGCA GTTATCTTCA GTTTTATTTT 
     3241 CTGCTCCAGA TGGTAATGAG AATGTTCAAG AGCAGCTTAG TGCAGATATT TTTCAACAAG 
     3301 TCAGTCAAAT TCAGAGTGGT GTAAGCCCTG GAATGTTTTC CTCAACAGAG CCAACAGTCC 
     3361 ATACCAGACC AGATAATTTA TTACCTGGAA GAGCTGAAAG TGTTCATCCA CAGTCTGAAA 
     3421 ACACGTTATC TAATCAACAG CAGCAGCAGC AGCAGCAACA GCAAGTGATG GAATCTTCAG 
     3481 CCGCAATGGT GATGGAGATG CAACAGAGTA TCTGCCAGGC AGCTGCCCAG ATTCAGTCAG 
     3541 AGTTATTCCC TTCAACTGCT TCAGCAAATG GAAACCTTCA GCAATCGCCA GTTTACCAGC 
     3601 AGACTTCTCA CATGATGAGT GCATTGTCTA CCAATGAGGA TATGCAAATG CAGTGTGAAT 
     3661 TGTTTTCTTC TCCTCCTGCA GTTTCTGGAA ATGAAACTTC TACAACTACC ACACAGCAGG 
     3721 TTGCAACCCC TGGCACTACC ATGTTTCAGA CATCAAGTTC AGGAGATGGA GAAGAAACTG 
     3781 GAACACAAGC AAAACAGATT CAGAACAGTG TCTTTCAGAC CATGGTCCAA ATGCAACATA 
     3841 GTGGGGACAA TCAACCTCAA GTTAACCTTT TTTCATCCAC AAAAAGTATG ATGAGTGTTC 
     3901 AGAATAGTGG TACCCAACAA CAAGGTAATG GTTTATTCCA GCAAGGGAAT GAGATGATGT 
     3961 CACTTCAATC TGGAAATTTT TTGCAGCAGT CTTCTCATTC ACAGGCCCAA CTTTTTCATC 
     4021 CTCAAAATCC TATTGCCGAT GCTCAGAACC TTTCCCAGGA AACTCAAGGT TCTCTCTTTC 
     4081 ATAGTCCAAA TCCTATTGTC CACAGTCAGA CTTCTACAAC CTCCTCTGAA CAAATGCAGC 
     4141 CTCCAATGTT TCACTCTCAA AGTACCATTG CTGTGTTACA GGGCTCTTCA GTTCCTCAAG 
     4201 ACCAGCAGTC AACCAACATA TTTCTTTCCC AGAGTCCCAT GAATAATCTT CAGACTAACA 
     4261 CAGTAGCCCA AGAAGCATTT TTTGCAGCAC CGAACTCAAT TTCTCCACTT CAGTCAACAT 
     4321 CAAACAGTGA ACAACAAGCT GCTTTCCAAC AGCAAGCTCC AATATCACAC ATCCAGACTC 
     4381 CTATGCTTTC CCAAGAACAG GCACAACCCC CGCAGCAGGG TTTATTTCAG CCTCAGGTGG 
     4441 CCCTGGGCTC CCTTCCACCT AATCCAATGC CTCAAAGCCA ACAAGGAACC ATGTTCCAGT 
     4501 CACAGCACTC AATAGTTGCC ATGCAGAGTA ACTCTCCATC CCAGGAACAG CAGCAGCAGC 
     4561 AGCAACAGCA GCAGCAACAG CAGCAGCAAC AACAACAGAG CATTTTATTC AGTAATCAGA 
     4621 ATACCATGGC TACAATGGCG TCTCCAAAGC AACCACCACC AAACATGATA TTCAACCCAA 
     4681 ATCAAAATCC AATGGCTAAT CAGGAGCAAC AGAACCAGTC AATTTTTCAC CAACAAAGTA 
     4741 ACATGGCCCC AATGAATCAA GAGCAACAGC CCATGCAATT TCAGAGTCAG TCCACAGTTT 
     4801 CCTCACTTCA GAACCCAGGT CCTACCCAGT CGGAATCATC ACAGACCCCC TTGTTCCATA 
     4861 GCTCTCCTCA GATTCAGTTG GTACAAGGGT CACCTAGTTC TCAAGAGCAG CAAGTAACTC 
     4921 TCTTCTTATC TCCAGCATCC ATGTCTGCCT TGCAGACCAG TATAAATCAA CAAGATATGC 
     4981 AACAGTCTCC TCTTTATTCC CCTCAGAACA ACATGCCTGG AATTCAAGGA GCCACATCTT 
     5041 CGCCTCAACC ACAGGCTACT TTATTTCACA ACACAGCAGG AGGCACAATG AACCAACTGC 
     5101 AGAATTCTCC TGGCTCATCT CAGCAGACAT CAGGAATGTT CTTATTTGGC ATTCAAAATA 
     5161 ACTGTAGTCA GCTTTTAACC TCTGGACCAG CTACATTGCC TGATCAGTTG ATGGCCATAA 
     5221 GTCAGCCAGG CCAACCACAA AACGAGGGCC AGCCACCTGT GACAACACTT CTTTCTCAGC 
     5281 AAATGCCAGA GAATTCTCCA CTGGCATCCT CTATAAACAC CAACCAGAAC ATCGAAAAGA 
     5341 TTGATTTGCT TGTTTCATTG CAAAACCAAG GGAACAACTT GACTGGCTCC TTTACGCGTA 
     5401 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5461 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5521 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5581 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5641 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5701 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5761 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5821 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5881 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5941 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     6001 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     6061 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     6121 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6181 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6241 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6301 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6361 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6421 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6481 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6541 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6601 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6661 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6721 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6781 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6841 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6901 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6961 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     7021 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     7081 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     7141 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7201 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7261 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7321 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7381 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7441 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7501 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7561 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7621 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7681 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7741 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7801 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7861 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7921 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7981 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     8041 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     8101 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     8161 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8221 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8281 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8341 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8401 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8461 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8521 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8581 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8641 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8701 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8761 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8821 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8881 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8941 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     9001 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     9061 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     9121 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9181 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9241 AATTTT
//