LOCUS       RC218211                8454 bp    DNA     circular  SYN 
DEFINITION  ARHGAP20 (Myc-DDK-tagged)-Human Rho GTPase activating protein 20 (ARHGAP20)
ACCESSION   NM_020809
VERSION     NM_020809.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4611..4702 
                     /label="Myc-DDK"  
     misc_feature    4732..5317 
                     /label="PolyA signal"  
     misc_feature    4789..4808 
                     /label="Restriction Enzyme"  
     misc_feature    5593..6265 
                     /label="ColE1"  
     misc_feature    6695..7489 
                     /label="Kan/Neo"  
     misc_feature    7524..7826 
                     /label="SV40 ori"  
     misc_feature    7912..8389 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4593..4599 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4593 
                     /label="ARHGAP20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAGCGATG TCCCCCCAGC AGGAGACTCT AGGGGGACAG CCGGGGCGCT 
     1081 CCTCTTCCCT GACAGGAGTG TCTCGGCTCG CGGGAGGCAG CTGCACCAAG AAGAAAATGA 
     1141 AAACACTAGC AGAAAGGAGG AGGAGCGCTC CATCTCTTAT CCTGGATAAA GCCCTACAAA 
     1201 AACGGCCTAC TACCAGGGAC AGTCCTTCTG CTAGTGTTGA CACATGCACA TTTCTGTCAT 
     1261 CATTAGTGTG CTCCAATAGG ACTCTGCTGA TTGATGGCCG GGCAGAACTC AAAAGAGGCC 
     1321 TCCAGAGGCA GGAGCGGCAT CTTTTCCTAT TCAATGATCT GTTTGTTGTG GCCAAAATCA 
     1381 AATATAACAA TAACTTTAAG ATAAAAAATA AAATTAAATT AACTGATATG TGGACAGCAA 
     1441 GCTGTGTGGA TGAAGTGGGA GAAGGCAACA CCAATGCCAT GAAATCCTTT GTTTTGGGCT 
     1501 GGCCCACAGT GAACTTTGTG GCCACTTTCA GTTCTCCAGA ACAAAAGGAC AAATGGCTCT 
     1561 CTCTCCTTCA GAGATACATC AATCTAGAGA AAGAAAAGGA CTACCCGAAG AGCATTCCCC 
     1621 TCAAAATCTT CGCCAAGGAC ATTGGGAATT GTGCCTACTC TAAAACTATA ACAGTAATGA 
     1681 ATTCAGATAC AGCGAATGAA GTTATCAACA TGTCATTACC AATGCTAGGG ATAACTGGCT 
     1741 CTGAGAGAGA TTACCAGTTG TGGGTCAATT CTGGCAAAGA AGAGGCTCCA TACCCACTCA 
     1801 TTGGGCATGA ATATCCATAT GGAATTAAAA TGAGCCATCT TCGAGACTCT GCACTCCTGA 
     1861 CACCGGGATC AAAGGACTCT ACCACCCCTT TCAACCTCCA GGAGCCCTTC CTTATGGAAC 
     1921 AGCTCCCCCG AGAGATGCAG TGCCAGTTCA TCCTGAAGCC CAGCCGCCTG GCTGCAGCCC 
     1981 AGCAACTGAG TGATTCAGGT CATAAGACAT TTAAAAGGAG AAGATCTATC ATAAACTGGG 
     2041 CCTTCTGGCG AGGTTCTAGC ACTCACCTGG ACAACTTGCC CTCATCGCCA ACATCACCTA 
     2101 TGCCAGGACA GCTCTTTGGA ATTTCTCTGC CAAATATTTG TGAGAATGAC AATCTGCCCA 
     2161 AACCTGTCTT GGATATGCTT TTCTTTCTTA ATCAAAAAGG ACCTCTCACC AAAGGTATCT 
     2221 TCAGGCAATC AGCCAATGTG AAATCCTGCA GAGAACTAAA AGAGAAATTG AATTCTGGAG 
     2281 TCGAAGTACA CCTAGACTGT GAATCTATTT TTGTGATAGC ATCTGTCTTA AAGGATTTTC 
     2341 TGCGAAATAT TCCAGGAAGT ATTTTTTCAT CAGATCTCTA TGATCACTGG GTCTCTGTAA 
     2401 TGGATCAAGG AAATGATGAA GAGAAAATAA ATACTGTTCA AAGGCTATTA GACCAGCTTC 
     2461 CGAGAGCCAA TGTTGTTCTC CTAAGGTATC TTTTTGGGGT GTTACACAAC ATTGAGCAAC 
     2521 ATTCCTCATC CAATCAGATG ACTGCATTTA ATTTAGCTGT GTGTGTCGCT CCAAGTATTC 
     2581 TTTGGCCTCC TGCTTCCTCC AGCCCAGAAC TAGAAAACGA ATTTACAAAA AAGGTTTCCC 
     2641 TGCTTATACA ATTTCTGATT GAGAATTGCC TTAGGATATT TGGAGAAGAA ATCACTTCCC 
     2701 TCTTCAGAGA GGTTTCAGTG AGATGTGACA CTAGAGAGAA TGCCTCAGAT ATTTCTTGCT 
     2761 TTCAACTGAA TGACTCCTCC TATGACAGCT TGGAAAATGA GCTAAATGAG GATGTTGATG 
     2821 CACCATGCAG TGACTTGGTA AAGAAACTTG GCCAGGGGAG CAGAAGCATG GACTCTGTCT 
     2881 TAACCCTCAG TGACTATGAT CTTGACCAGC CCGAGGTGGA AGGCCTTTTA ACCCTAAGCG 
     2941 ACTTTGACTT GGCCCATTCT AAAGATGAAG ATGTTCAAAT GAAACGGCCT CTTGAATCCA 
     3001 AGCCGGTGAA CATTTTAGTG TACACAAAGA TCCCACTGCG GGATCATGCC AGGGCCCCAT 
     3061 CTGCCATGTG CACACCCAGC TACCTGTCCA CAGCTGCAGC AAATGCTGCA AAAAGCCTGA 
     3121 GGCGACACCG GCGTTGCTCA GAGCCCAGCA TCGACTATCT GGATTCAAAG CTTTCCTACC 
     3181 TCAGGGAGTT TTATCAGAAA AAGCTACGCA AGTCCAGCTG TGATGCAATT CTTTCTCAAA 
     3241 AAGATGAAGA CTATCTGAAG CAGAATCAAC CCCTCCAGGA GGAAGGAAAG ACATGTTTTA 
     3301 AACAGAGTTT AGTCACAGGC ACTGATGTCA GCAAGAAAAA TGCCACTACT CAAAACACTA 
     3361 AGAAGAAAAG CTTGTCTGGT AGTGAAGGAA ATCACGTGAA ACTTTTCCCT AAGTCTAAGC 
     3421 CAGTGGCCAT TTCTGTGGCA TCTTATAGTC CTATGTCCTC ACAGGATCAT TCCAAGAACC 
     3481 AGCCCTTTGA TGTGAATACA TCTGGATACT CCCCACCACA CACAGCAGAT GCCCTCAAGG 
     3541 GTCCAAGGAC ACATCGGCGC TGCTCAGAGC CCAACATAGA AGACCAGAAC CGCAAGCTGA 
     3601 CCTATCTCAG GGGAATTTAT TCAAAGAAAC AACATAAAAC CAGCTGTGAA GCTGGTCTCT 
     3661 TGCATGGAGA GGAGGATTAT CTCAAACGGC ATAAGTCTTT GCAAATGGAG GGGCAGAAGC 
     3721 TCATTAATCA GAGTTTAGTC ATGGGGATTG AGGTGGGCAA GAGTAGTGCC ACAAACCAAA 
     3781 ACACTGAGAA GGTTTTACCC CCAAGATTAA ACCTTTGCCC AAGGACCAGC TATTCCAGCT 
     3841 TATCCTCCCC AGGCACTTCC CCATCCGGCT CATCAGTAAG CTCCCAAGAC AGTGCTTTTT 
     3901 CTCAGATTTC TGAACACTCT GTGTTTACAC CCACTGAGAC TTCCTCTCCA ATAGATTGCA 
     3961 CTTTTCAGGC TCAGAGAAAA CGGGAAGACC TTTCTCCTGA CTTTAGCAAT GCCAGCCATG 
     4021 TTTCCGGAAT GCCCGGTCCC TCATCAGGGC AGGCTTGCAG CCGCCCAGCC TATACAAAGA 
     4081 AGGACACCAT GGAGTGGCAT TCACAAATGC ATTCTGTAAC TCTTCATCCC AGCACATGGT 
     4141 TGAGAAATGG TGTGGCCAGT TTGAAAAACT GGTCCCTCAA AAAGAAAGCA AAGGCAGCCA 
     4201 GACCAGAGGA AGAGAAAATA GCTTCTCCAA AAGGACCCTT AGAGCCACCC CCACATGCTT 
     4261 CTGGTGTTCC AGAAGCCAAC TCACTGCAAG AGGAACAAAA AGACTTGCCC TTAAGGGCAG 
     4321 CTGAAGGACT GTCCCCTGTG CAGTCAGCCC AAAGGTGTAG TTCTTCTCCC TTCCAGGACT 
     4381 CAGAGAGACA CTGTAGCTCT CCATTCAGCC TGGTGGAGAG CAGACTTAAG CTGTGCATGA 
     4441 AGTCACATGA GGAAATAGAG CCTGGTAGTC AGAGCTCTTC TGGTTCTCTG CCTTGGGAAA 
     4501 GAGCCTCAGC CAGCTCTTGG ACTCTAGAGG ATGCGACCAG CCCAGACTCA GGGCCTACAG 
     4561 TGGTCTGCGA CATTGAGGAC AGGTATTTAA CCAAAGACAT TACGCGTACG CGGCCGCTCG 
     4621 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4681 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4741 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4801 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4861 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4921 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4981 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5041 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5101 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5161 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5221 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5281 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5341 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5401 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5461 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5521 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5581 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5641 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5701 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5761 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5821 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5881 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5941 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6001 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6061 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6121 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6181 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6241 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6301 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6361 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6421 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6481 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6541 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6601 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6661 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6721 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6781 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6841 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6901 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6961 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7021 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7081 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7141 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7201 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7261 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7321 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7381 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7441 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7501 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7561 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7621 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7681 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7741 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7861 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7921 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7981 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8041 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8101 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8161 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8221 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8281 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8341 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8401 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//