LOCUS       RC218160                8877 bp    DNA     circular  SYN 
DEFINITION  SOS2 (Myc-DDK-tagged)-Human son of sevenless homolog 2 (Drosophila) (SOS2)
ACCESSION   NM_006939
VERSION     NM_006939.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5034..5125 
                     /label="Myc-DDK"  
     misc_feature    5155..5740 
                     /label="PolyA signal"  
     misc_feature    5212..5231 
                     /label="Restriction Enzyme"  
     misc_feature    6016..6688 
                     /label="ColE1"  
     misc_feature    7118..7912 
                     /label="Kan/Neo"  
     misc_feature    7947..8249 
                     /label="SV40 ori"  
     misc_feature    8335..8812 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5016..5022 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5016 
                     /label="SOS2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCAGGCG CCGCAGCCTT ACGAGTTCTT CAGCGAGGAG AACAGTCCGA 
     1081 AATGGCGGGG ACTGTTGGTC TCGGCCCTGC GGAAGGTTCA GGAACAAGTG CATCCCACTC 
     1141 TCTCAGCTAA TGAAGAGTCT CTCTATTATA TTGAAGAGCT GATTTTTCAG CTGCTTAATA 
     1201 AATTATGCAT GGCCCAGCCA AGGACTGTTC AAGATGTAGA GGAGCGAGTT CAGAAGACCT 
     1261 TTCCTCACCC AATTGATAAA TGGGCCATTG CTGATGCACA ATCTGCTATA GAAAAACGAA 
     1321 AACGAAGAAA TCCTCTTTTA CTGCCTGTGG ACAAAATCCA TCCTTCGTTG AAGGAAGTAT 
     1381 TAGGGTACAA AGTGGACTAC CATGTATCCC TATATATTGT GGCTGTACTA GAGTATATCT 
     1441 CAGCTGATAT TTTAAAATTG GCTGGTAATT ATGTTTTTAA TATCCGGCAT TATGAAATAT 
     1501 CTCAGCAGGA CATTAAAGTG TCAATGTGTG CGGATAAGGT TTTGATGGAC ATGTTTGATC 
     1561 AGGATGACAT AGGTTTGGTT TCTCTCTGTG AAGATGAACC TAGTTCTTCT GGTGAATTAA 
     1621 ACTACTATGA TCTTGTCAGA ACTGAAATCG CAGAAGAAAG ACAGTATCTA CGGGAATTAA 
     1681 ATATGATCAT AAAAGTGTTT CGAGAAGCCT TTCTTTCTGA TAGAAAGCTG TTTAAACCTT 
     1741 CTGATATCGA AAAGATTTTT AGTAACATTT CAGATATACA TGAATTGACT GTGAAACTTT 
     1801 TAGGTTTGAT TGAAGACACA GTTGAAATGA CTGATGAAAG CAGTCCTCAT CCCTTAGCTG 
     1861 GCAGCTGTTT TGAAGATTTG GCAGAAGAGC AAGCATTTGA TCCTTATGAA ACATTATCAC 
     1921 AGGACATTCT TTCACCAGAG TTTCATGAAC ATTTCAATAA ATTGATGGCC AGACCTGCAG 
     1981 TTGCTCTACA CTTTCAGTCC ATTGCTGATG GTTTTAAAGA GGCAGTTCGT TATGTCCTTC 
     2041 CACGTCTTAT GCTGGTGCCA GTGTATCACT GTTGGCACTA CTTTGAGTTA CTAAAGCAAT 
     2101 TGAAAGCATG TAGTGAAGAA CAAGAAGACA GAGAATGTTT GAACCAAGCT ATTACTGCTC 
     2161 TCATGAATCT CCAAGGTAGC ATGGACCGAA TTTACAAGCA GTATTCACCT AGACGTCGAC 
     2221 CTGGAGATCC TGTTTGCCCT TTTTATAGTC ACCAATTAAG AAGCAAACAC CTGGCTATCA 
     2281 AAAAAATGAA TGAAATTCAG AAAAATATCG ATGGATGGGA AGGCAAAGAT ATTGGACAGT 
     2341 GTTGTAATGA ATTCATTATG GAGGGACCAT TGACAAGAAT CGGTGCCAAA CATGAACGGC 
     2401 ATATTTTTCT GTTTGATGGC TTAATGATCA GTTGTAAACC TAATCATGGC CAGACTCGGC 
     2461 TTCCAGGTTA CAGTAGTGCA GAATACAGGT TAAAAGAAAA ATTTGTCATG AGGAAAATAC 
     2521 AAATTTGTGA TAAAGAAGAT ACTTGTGAGC ACAAGCATGC ATTTGAATTA GTATCCAAAG 
     2581 ATGAGAACAG CATAATATTT GCTGCTAAGT CTGCTGAAGA AAAAAACAAC TGGATGGCAG 
     2641 CCCTTATTTC TCTTCATTAT CGTAGTACTC TAGATCGAAT GTTAGATTCA GTATTATTGA 
     2701 AAGAAGAAAA TGAGCAACCA CTGAGATTAC CAAGTCCTGA AGTATATCGT TTTGTAGTAA 
     2761 AAGACTCTGA GGAAAACATT GTTTTTGAAG ACAACTTGCA AAGTAGAAGT GGCATCCCCA 
     2821 TTATTAAAGG AGGAACTGTA GTGAAATTAA TTGAAAGGTT AACATATCAT ATGTATGCAG 
     2881 ATCCCAATTT TGTTCGTACT TTTCTTACCA CATATCGTTC ATTTTGTAAA CCACAGGAAT 
     2941 TGCTGAGCTT ACTGATTGAA CGGTTTGAAA TTCCAGAGCC AGAACCTACT GACGCAGACA 
     3001 AATTGGCAAT AGAGAAAGGC GAGCAGCCAA TCAGTGCAGA CCTTAAAAGA TTTCGCAAGG 
     3061 AATATGTCCA ACCAGTACAA CTTAGGATCT TAAATGTATT TCGGCATTGG GTTGAACATC 
     3121 ATTTTTATGA CTTTGAAAGA GACTTGGAAT TGCTTGAAAG ACTAGAATCC TTCATTTCAA 
     3181 GTGTAAGAGG GAAAGCTATG AAAAAATGGG TAGAGTCAAT TGCTAAGATC ATCAGGAGGA 
     3241 AGAAGCAAGC TCAGGCAAAC GGAGTAAGCC ATAATATTAC CTTTGAAAGT CCACCTCCAC 
     3301 CAATTGAATG GCATATCAGC AAACCAGGAC AGTTTGAAAC ATTTGATCTC ATGACACTTC 
     3361 ATCCAATAGA AATTGCACGT CAGCTGACAC TTTTGGAGTC TGATCTTTAC AGGAAAGTTC 
     3421 AACCGTCTGA ACTTGTAGGG AGTGTGTGGA CCAAAGAAGA TAAAGAAATA AATTCTCCAA 
     3481 ATTTATTAAA AATGATTCGC CATACCACAA ATCTCACCCT CTGGTTTGAA AAATGCATTG 
     3541 TGGAAGCAGA AAATTTTGAA GAACGGGTGG CAGTACTAAG TAGAATTATA GAAATTCTGC 
     3601 AAGTTTTTCA AGATTTGAAT AATTTCAATG GCGTATTGGA GATAGTCAGT GCAGTAAATT 
     3661 CAGTGTCAGT ATACAGACTA GACCATACCT TTGAGGCACT GCAGGAAAGG AAAAGGAAAA 
     3721 TTTTGGACGA AGCTGTGGAA TTAAGTCAAG ATCACTTTAA AAAATACCTA GTAAAACTTA 
     3781 AGTCAATCAA TCCACCTTGT GTGCCTTTTT TTGGAATATA TTTAACAAAT ATTCTGAAGA 
     3841 CCGAAGAAGG GAATAATGAT TTTTTAAAAA AGAAAGGGAA AGATTTAATC AATTTCAGTA 
     3901 AGAGGAGGAA AGTAGCTGAA ATTACTGGAG AAATTCAGCA GTATCAGAAT CAGCCTTACT 
     3961 GTTTACGGAT AGAACCAGAT ATGAGGAGAT TCTTTGAAAA CCTTAACCCC ATGGGAAGTG 
     4021 CATCTGAAAA AGAGTTTACA GATTATTTGT TCAACAAGTC ACTAGAAATT GAACCTCGAA 
     4081 ACTGCAAACA GCCACCTCGA TTTCCTAGGA AATCAACTTT TTCCTTAAAA TCTCCTGGAA 
     4141 TAAGGCCTAA CACAGGCCGA CATGGCTCTA CCTCAGGTAC TTTACGAGGT CACCCAACAC 
     4201 CATTAGAAAG AGAACCATGT AAAATAAGCT TTAGTCGGAT TGCTGAAACT GAGCTGGAAT 
     4261 CAACAGTGTC AGCACCAACC TCTCCAAATA CACCATCTAC TCCACCAGTA TCTGCTTCTT 
     4321 CAGACCTTAG TGTATTTTTA GATGTGGATC TCAACAGCTC CTGTGGCAGC AATAGCATCT 
     4381 TTGCTCCAGT GCTTTTGCCA CATTCAAAGT CTTTCTTTAG TTCATGTGGT AGTTTACATA 
     4441 AACTAAGTGA AGAGCCCCTG ATTCCTCCTC CTCTTCCTCC TCGAAAAAAG TTTGATCATG 
     4501 ATGCTTCAAA TTCCAAGGGA AATATGAAAT CTGATGATGA TCCTCCTGCT ATTCCACCGA 
     4561 GACAGCCTCC TCCTCCAAAG GTAAAACCCA GAGTTCCTGT TCCTACTGGT GCATTTGATG 
     4621 GGCCTCTGCA TAGTCCACCT CCGCCACCAC CAAGAGATCC TCTTCCTGAT ACCCCTCCAC 
     4681 CAGTTCCCCT TCGGCCTCCA GAACACTTTA TAAACTGTCC ATTTAATCTT CAGCCACCTC 
     4741 CACTGGGGCA TCTTCACAGA GATTCAGACT GGCTCAGAGA CATTAGTACG TGTCCAAATT 
     4801 CGCCAAGCAC TCCTCCTAGC ACACCCTCTC CAAGGGTACC GCGTCGATGC TATGTGCTCA 
     4861 GTTCTAGTCA GAATAATCTT GCTCATCCTC CAGCTCCCCC TGTTCCACCA AGGCAGAATT 
     4921 CAAGCCCTCA TCTGCCAAAA CTGCCACCAA AGACTTACAA ACGGGAGCTT TCGCACCCCC 
     4981 CATTGTACAG ACTGCCTTTG CTAGAAAATG CAGAAACTCC CCAAACGCGT ACGCGGCCGC 
     5041 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5101 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5161 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5221 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5281 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5341 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5401 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5461 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5521 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5581 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5641 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5701 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5761 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5821 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5881 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5941 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6001 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6061 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6121 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6181 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6241 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6301 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6361 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6421 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6481 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6541 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6601 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6661 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6721 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6781 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6841 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6901 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6961 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7021 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7081 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7141 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7201 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7261 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7321 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7381 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7441 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7501 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7561 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7621 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7681 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7741 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7801 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7861 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7921 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7981 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8041 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8101 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8161 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8221 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8281 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8341 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8401 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8461 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8521 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8581 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8641 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8701 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8761 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8821 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//