LOCUS       RC218143                8259 bp    DNA     circular  SYN 
DEFINITION  ARHGAP33 (Myc-DDK-tagged)-Human Rho GTPase activating protein 33 (ARHGAP33), transcript variant 1
ACCESSION   NM_052948
VERSION     NM_052948.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4416..4507 
                     /label="Myc-DDK"  
     misc_feature    4537..5122 
                     /label="PolyA signal"  
     misc_feature    4594..4613 
                     /label="Restriction Enzyme"  
     misc_feature    5398..6070 
                     /label="ColE1"  
     misc_feature    6500..7294 
                     /label="Kan/Neo"  
     misc_feature    7329..7631 
                     /label="SV40 ori"  
     misc_feature    7717..8194 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4398..4404 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4398 
                     /label="ARHGAP33"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTGGCACGC AGCACTGACA GCCTGGATGG CCCAGGGGAG GGCTCGGTGC 
     1081 AGCCTCTACC CACTGCTGGG GGGCCCAGTG TGAAGGGGAA GCCTGGGAAG AGGCTCTCAG 
     1141 CTCCTCGAGG CCCCTTCCCG CGGCTGGCTG ACTGCGCCCA TTTCCACTAC GAGAACGTTG 
     1201 ACTTTGGCCA CATTCAGCTC CTGCTGTCTC CAGACCGTGA AGGGCCCAGC CTCTCTGGAG 
     1261 AGAATGAGCT GGTGTTCGGG GTGCAGGTGA CCTGTCAGGG CCGTTCCTGG CCGGTTCTCC 
     1321 GGAGTTACGA TGACTTTCGT TCCCTGGATG CCCACCTCCA CCGGTGCATA TTTGACCGGA 
     1381 GGTTCTCCTG CCTTCCGGAG CTTCCCCCGC CCCCCGAGGG TGCCAGGGCT GCCCAGATGC 
     1441 TGGTGCCACT GCTGCTGCAG TACCTGGAGA CACTGTCAGG ACTGGTGGAC AGTAACCTCA 
     1501 ACTGCGGGCC TGTGCTCACC TGGATGGAGC TGGACAATCA CGGCCGGCGA CTGCTCCTCA 
     1561 GTGAGGAGGC GTCACTCAAT ATCCCTGCAG TGGCGGCCGC CCATGTGATC AAACGGTATA 
     1621 CAGCCCAGGC GCCAGATGAG CTGTCCTTTG AGGTGGGAGA CATTGTCTCG GTGATCGACA 
     1681 TGCCACCCAC AGAGGATCGG AGCTGGTGGC GGGGCAAGCG AGGCTTCCAG GTCGGGTTCT 
     1741 TCCCCAGTGA GTGTGTGGAA CTCTTCACAG AGCGGCCAGG TCCGGGCCTG AAGGCGGATG 
     1801 CCGATGGCCC CCCATGTGGC ATCCCGGCTC CCCAGGGTAT CTCGTCTCTG ACCTCAGCTG 
     1861 TGCCACGGCC TCGTGGGAAG CTGGCCGGCC TGCTCCGCAC CTTCATGCGC TCCCGCCCTT 
     1921 CTCGGCAGCG GCTGCGGCAG CGGGGAATCC TGCGACAGAG GGTGTTTGGC TGCGATCTTG 
     1981 GCGAGCACCT CAGCAACTCA GGCCAGGATG TGCCCCAGGT GCTGCGCTGC TGCTCCGAGT 
     2041 TCATTGAGGC CCACGGGGTG GTGGATGGGA TCTACCGGCT CTCAGGCGTG TCTTCCAACA 
     2101 TCCAGAGGCT TCGGCACGAG TTTGACAGTG AGAGGATCCC GGAGCTGTCT GGCCCTGCAT 
     2161 TCCTGCAGGA CATCCACAGC GTGTCCTCCC TCTGCAAGCT CTACTTCCGA GAGCTTCCGA 
     2221 ACCCTCTGCT CACCTACCAG CTCTATGGGA AGTTCAGTGA GGCCATGTCA GTGCCTGGGG 
     2281 AGGAGGAGCG TCTGGTGCGG GTGCACGATG TCATCCAGCA GCTGCCCCCA CCACATTACA 
     2341 GGACCCTGGA GTACCTGCTG AGGCACCTGG CCCGCATGGC GAGACACAGT GCCAACACCA 
     2401 GCATGCATGC CCGCAACCTG GCCATTGTCT GGGCACCCAA CCTGCTACGG TCCATGGAGC 
     2461 TGGAGTCAGT GGGAATGGGT GGCGCGGCGG CGTTCCGGGA AGTTCGGGTG CAGTCGGTGG 
     2521 TGGTGGAGTT TCTGCTCACC CATGTGGACG TCCTGTTCAG CGACACCTTC ACCTCCGCCG 
     2581 GCCTCGACCC TGCAGGCCGC TGCCTGCTCC CCAGGCCCAA GTCCCTTGCG GGCAGCTGCC 
     2641 CCTCCACCCG CCTGCTGACG CTGGAGGAAG CCCAGGCACG CACCCAGGGC CGGCTGGGGA 
     2701 CGCCCACGGA GCCCACAACT CCCAAGGCCC CGGCCTCACC TGCGGAAAGG AGGAAAGGGG 
     2761 AGAGAGGGGA GAAGCAGCGG AAGCCAGGGG GCAGCAGCTG GAAGACGTTC TTTGCACTGG 
     2821 GCCGGGGCCC CAGTGTCCCT CGAAAGAAGC CCCTGCCCTG GCTGGGGGGC ACCCGTGCCC 
     2881 CACCGCAGCC TTCAGGCAGC AGACCCGACA CCGTCACACT GAGATCTGCC AAGAGCGAGG 
     2941 AGTCTCTGTC ATCGCAGGCC AGCGGGGCTG AACTGCTGGG GGCTGGGGGA GCACCTGCCT 
     3001 CAGCCACCCC AACACCAGCT CTCAGCCCCG GCCGGAGCCT GCGCCCCCAT CTCATACCCC 
     3061 TGCTGCTGCG AGGAGCCGAG GCCCCGCTGA CTGACGCCTG CCAGCAGGAG ATGTGCAGCA 
     3121 AGCTCCGGGG AGCCCAGGGC CCACTCGGTC CTGATATGGA GTCACCACTG CCACCCCCTC 
     3181 CCCTGTCTCT CCTGCGCCCT GGGGGTGCCC CACCCCCGCC CCCTAAGAAC CCAGCACGCC 
     3241 TCATGGCCCT GGCCCTGGCT GAGCGGGCTC AGCAGGTGGC CGAGCAACAG AGCCAGCAGG 
     3301 AGTGTGGGGG CACCCCACCT GCTTCCCAAT CCCCCTTCCA CCGCTCGCTG TCTCTGGAGG 
     3361 TGGGCGGGGA GCCCCTGGGG ACCTCAGGGA GTGGGCCACC TCCCAACTCC CTAGCACACC 
     3421 CGGGTGCCTG GGTCCCGGGA CCCCCACCCT ACTTACCAAG GCAACAAAGT GATGGGAGCC 
     3481 TGCTGAGGAG CCAGCGGCCC ATGGGGACCT CAAGGAGGGG ACTCCGAGGC CCTGCCCAGG 
     3541 TCAGTGCCCA GCTCAGGGCA GGTGGCGGGG GCAGGGATGC GCCAGAGGCA GCAGCCCAGT 
     3601 CCCCATGTTC TGTCCCCTCA CAGGTTCCTA CCCCCGGCTT CTTCTCCCCA GCCCCCAGGG 
     3661 AGTGCCTGCC ACCCTTCCTC GGGGTCCCCA AGCCAGGCTT GTACCCCCTG GGCCCCCCAT 
     3721 CCTTCCAGCC CAGTTCCCCA GCCCCAGTCT GGAGGAGCTC TCTGGGCCCC CCTGCACCAC 
     3781 TCGACAGGGG AGAGAACCTG TACTATGAGA TCGGGGCAAG TGAGGGGTCC CCCTATTCTG 
     3841 GCCCCACCCG CTCCTGGAGT CCCTTTCGCT CCATGCCCCC CGACAGGCTC AATGCCTCCT 
     3901 ACGGCATGCT TGGCCAATCA CCCCCACTCC ACAGGTCCCC CGACTTCCTG CTCAGCTACC 
     3961 CGCCAGCCCC CTCCTGCTTT CCCCCTGACC ACCTTGGCTA CTCAGCCCCC CAGCACCCTG 
     4021 CTCGGCGCCC TACACCGCCT GAGCCCCTCT ACGTCAACCT AGCTCTAGGG CCCAGGGGTC 
     4081 CCTCACCTGC CTCTTCCTCC TCCTCTTCCC CTCCTGCCCA CCCCCGAAGC CGTTCAGATC 
     4141 CCGGTCCCCC AGTCCCCCGC CTTCCCCAGA AACAACGGGC ACCCTGGGGA CCCCGTACCC 
     4201 CTCATAGGGT GCCGGGTCCC TGGGGCCCTC CTGAGCCTCT CCTGCTCTAC AGGGCAGCCC 
     4261 CGCCAGCCTA CGGAAGGGGG GGCGAGCTCC ACCGAGGGTC CTTGTACAGA AATGGAGGGC 
     4321 AAAGAGGGGA GGGGGCTGGT CCCCCACCCC CTTACCCCAC TCCCAGCTGG TCCCTCCACT 
     4381 CTGAGGGCCA GACCCGAAGC TACTGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4441 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4501 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4561 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4621 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4681 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4741 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4801 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4861 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4921 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4981 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5041 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5101 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5161 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5221 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5281 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5341 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5401 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5461 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5521 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5581 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5641 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5701 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5761 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5821 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5881 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5941 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6001 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6061 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6121 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6181 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6241 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6301 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6361 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6421 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6481 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6541 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6601 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6661 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6721 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6781 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6841 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6901 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6961 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7021 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7081 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7141 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7201 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7261 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7321 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7381 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7441 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7501 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7561 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7621 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7681 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7741 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7801 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7861 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7921 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7981 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8041 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8101 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8161 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8221 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//