LOCUS       RC218130                7644 bp    DNA     circular  SYN 
DEFINITION  COL9A1 (Myc-DDK-tagged)-Human collagen, type IX, alpha 1 (COL9A1), transcript variant 1
ACCESSION   NM_001851
VERSION     NM_001851.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3801..3892 
                     /label="Myc-DDK"  
     misc_feature    3922..4507 
                     /label="PolyA signal"  
     misc_feature    3979..3998 
                     /label="Restriction Enzyme"  
     misc_feature    4783..5455 
                     /label="ColE1"  
     misc_feature    5885..6679 
                     /label="Kan/Neo"  
     misc_feature    6714..7016 
                     /label="SV40 ori"  
     misc_feature    7102..7579 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3783..3789 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3783 
                     /label="COL9A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGACCTGC TGGAAAATTC CAGTTTTCTT CTTTGTGTGC AGTTTCCTGG 
     1081 AACCCTGGGC ATCTGCAGCT GTCAAGCGTC GCCCCAGATT CCCTGTCAAT TCCAATTCTA 
     1141 ATGGTGGAAA TGAACTCTGT CCAAAGATCA GGATTGGCCA AGATGACTTA CCAGGGTTTG 
     1201 ATCTGATCTC TCAGTTCCAG GTAGATAAAG CAGCATCTAG AAGAGCTATC CAGAGAGTAG 
     1261 TGGGATCAGC TACATTGCAG GTGGCTTACA AGTTGGGAAA TAATGTAGAC TTCAGGATTC 
     1321 CAACTAGGAA TTTATATCCC AGTGGACTGC CTGAAGAATA CTCCTTCTTG ACGACGTTTC 
     1381 GAATGACTGG AAGCACTCTC AAAAAGAACT GGAACATTTG GCAGATTCAG GATTCCTCTG 
     1441 GGAAGGAGCA AGTTGGCATA AAGATTAATG GCCAAACACA ATCTGTTGTA TTTTCATACA 
     1501 AGGGACTGGA TGGAAGTCTC CAAACAGCAG CCTTTTCGAA TTTGTCCTCC TTGTTTGATT 
     1561 CCCAGTGGCA TAAGATCATG ATTGGCGTGG AGAGGAGTAG TGCTACTCTT TTTGTTGACT 
     1621 GCAACAGGAT TGAATCTTTA CCTATAAAGC CAAGAGGCCC AATTGACATT GATGGCTTTG 
     1681 CTGTGCTGGG AAAACTTGCA GATAATCCTC AAGTTTCTGT TCCATTTGAA CTTCAATGGA 
     1741 TGCTGATCCA TTGTGACCCC CTGCGGCCCA GGAGAGAAAC TTGCCATGAG CTGCCAGCCA 
     1801 GAATAACGCC CAGCCAGACC ACCGACGAGA GAGGTCCCCC GGGTGAGCAG GGTCCTCCCG 
     1861 GGCCTCCGGG CCCCCCTGGA GTTCCAGGCA TCGATGGCAT CGACGGTGAC CGAGGTCCTA 
     1921 AGGGCCCCCC GGGCCCCCCG GGTCCTGCAG GTGAACCGGG AAAGCCAGGA GCTCCAGGCA 
     1981 AGCCTGGCAC ACCTGGCGCT GATGGATTAA CAGGACCTGA TGGATCCCCT GGCTCCATTG 
     2041 GGTCAAAGGG ACAAAAAGGA GAACCTGGTG TGCCTGGATC GCGTGGATTT CCAGGCCGTG 
     2101 GTATTCCTGG ACCCCCTGGT CCTCCTGGGA CAGCAGGACT CCCTGGAGAG CTTGGCCGTG 
     2161 TAGGACCTGT TGGTGACCCT GGGAGAAGAG GACCACCTGG CCCCCCTGGC CCCCCAGGAC 
     2221 CCAGAGGAAC AATTGGCTTT CATGATGGAG ATCCATTGTG TCCCAATGCC TGTCCACCAG 
     2281 GTCGCTCAGG ATATCCAGGC CTACCAGGCA TGAGGGGTCA TAAAGGGGCT AAAGGAGAAA 
     2341 TTGGTGAACC AGGAAGACAA GGACACAAGG GTGAAGAAGG TGACCAGGGA GAACTCGGAG 
     2401 AAGTTGGAGC TCAAGGACCT CCAGGAGCCC AGGGTTTGCG AGGCATCACC GGCATAGTTG 
     2461 GGGACAAAGG GGAAAAAGGT GCTCGGGGCT TAGATGGTGA ACCTGGGCCT CAGGGTCTTC 
     2521 CTGGTGCACC TGGTGATCAA GGACAGCGAG GACCTCCAGG AGAAGCAGGT CCCAAAGGAG 
     2581 ATAGAGGGGC TGAAGGTGCT AGAGGAATTC CTGGTCTCCC TGGGCCCAAA GGAGACACGG 
     2641 GTTTGCCAGG TGTGGATGGC CGTGATGGGA TCCCTGGAAT GCCTGGAACA AAGGGTGAAC 
     2701 CAGGAAAACC TGGGCCTCCT GGTGATGCAG GATTGCAGGG GTTACCAGGT GTACCTGGAA 
     2761 TTCCTGGTGC AAAGGGTGTT GCTGGTGAAA AGGGTAGCAC AGGTGCTCCA GGGAAGCCTG 
     2821 GTCAGATGGG AAATTCAGGC AAACCGGGCC AACAGGGGCC TCCAGGAGAG GTGGGACCCC 
     2881 GAGGACCCCA GGGGCTTCCT GGCAGTAGAG GAGAATTAGG ACCAGTGGGA TCCCCAGGCC 
     2941 TACCAGGTAA ACTGGGTTCT CTGGGTAGCC CTGGCCTCCC TGGCTTGCCT GGGCCCCCTG 
     3001 GACTTCCTGG AATGAAAGGT GACAGGGGTG TAGTCGGTGA ACCGGGTCCA AAGGGTGAAC 
     3061 AGGGTGCCTC TGGTGAAGAA GGTGAAGCAG GAGAAAGGGG GGAACTTGGA GATATAGGAT 
     3121 TACCTGGCCC AAAGGGATCT GCAGGTAATC CTGGGGAACC TGGCTTGAGA GGGCCTGAGG 
     3181 GAAGTCGGGG GCTTCCTGGA GTGGAAGGAC CAAGAGGACC ACCTGGACCC CGGGGTGTGC 
     3241 AGGGAGAACA GGGTGCCACC GGCCTGCCTG GTGTCCAGGG CCCTCCGGGT AGAGCACCGA 
     3301 CAGATCAGCA CATTAAGCAG GTTTGCATGA GAGTCATACA AGAACATTTT GCTGAGATGG 
     3361 CTGCCAGTCT TAAGCGTCCA GACTCAGGTG CCACTGGGCT TCCTGGAAGG CCTGGCCCTC 
     3421 CTGGTCCCCC CGGCCCTCCT GGAGAGAATG GTTTCCCAGG CCAGATGGGA ATTCGTGGCC 
     3481 TTCCGGGCAT TAAGGGGCCC CCTGGTGCTC TTGGTTTGAG GGGACCTAAA GGTGACTTGG 
     3541 GAGAAAAGGG GGAGCGTGGC CCTCCAGGAA GAGGTCCCAA CGGTTTGCCT GGAGCTATAG 
     3601 GTCTCCCAGG TGACCCAGGC CCTGCCAGCT ATGGCAGAAA TGGCCGAGAC GGTGAGCGAG 
     3661 GCCCCCCAGG GGTGGCAGGA ATTCCTGGAG TGCCTGGACC CCCGGGACCT CCTGGGCTTC 
     3721 CCGGTTTCTG TGAGCCAGCC TCCTGCACCA TGCAGGCTGG TCAGCGAGCA TTTAACAAAG 
     3781 GGCCTGACCC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3841 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3901 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3961 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4021 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4081 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4141 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4201 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4261 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4321 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4381 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4441 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4501 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4561 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4621 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4681 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4741 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4801 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4861 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4921 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4981 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5041 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5101 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5161 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5221 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5281 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5341 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5401 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5461 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5521 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5581 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5641 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5701 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5761 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5821 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5881 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5941 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6001 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6061 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6121 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6181 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6241 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6301 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6361 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6421 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6481 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6541 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6601 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6661 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6721 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6781 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6841 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6901 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6961 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7021 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7081 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7141 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7201 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7261 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7321 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7381 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7441 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7501 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7561 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7621 TAACAAAAAT TTAACGCGAA TTTT
//