LOCUS RC218103 7491 bp DNA circular SYN DEFINITION GRM2 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 2 (GRM2), transcript variant 1 ACCESSION NM_000839 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3646..3737 /label="Myc-DDK" misc_feature 3767..4352 /label="PolyA signal" misc_feature 3824..3843 /label="Restriction Enzyme" misc_feature 4628..5300 /label="ColE1" misc_feature 5730..6524 /label="Kan/Neo" misc_feature 6559..6861 /label="SV40 ori" misc_feature 6947..7424 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3636..3644 /label="Restriction Enzyme" misc_feature 1020..3636 /label="GRM2" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGGATCGCTG CTTGCGCTCC TGGCACTGCT GCTGCTGTGG GGTGCTGTGG 1081 CTGAGGGCCC AGCCAAGAAG GTGCTGACCC TGGAGGGAGA CTTGGTGCTG GGTGGGCTGT 1141 TCCCAGTGCA CCAGAAGGGC GGCCCAGCAG AGGACTGTGG TCCTGTCAAT GAGCACCGTG 1201 GCATCCAGCG CCTGGAGGCC ATGCTTTTTG CACTGGACCG CATCAACCGT GACCCGCACC 1261 TGCTGCCTGG CGTGCGCCTG GGTGCACACA TCCTCGACAG TTGCTCCAAG GACACACATG 1321 CGCTGGAGCA GGCACTGGAC TTTGTGCGTG CCTCACTCAG CCGTGGTGCT GATGGCTCAC 1381 GCCACATCTG CCCCGACGGC TCTTATGCGA CCCATGGTGA TGCTCCCACT GCCATCACTG 1441 GTGTTATTGG CGGTTCCTAC AGTGATGTCT CCATCCAGGT GGCCAACCTC TTGAGGCTAT 1501 TTCAGATCCC ACAGATTAGC TACGCCTCTA CCAGTGCCAA GCTGAGTGAC AAGTCCCGCT 1561 ATGACTACTT TGCCCGCACA GTGCCTCCTG ACTTCTTCCA AGCCAAGGCC ATGGCTGAGA 1621 TTCTCCGCTT CTTCAACTGG ACCTATGTGT CCACTGTGGC GTCTGAGGGC GACTATGGCG 1681 AGACAGGCAT TGAGGCCTTT GAGCTAGAGG CTCGTGCCCG CAACATCTGT GTGGCCACCT 1741 CGGAGAAAGT GGGCCGTGCC ATGAGCCGCG CGGCCTTTGA GGGTGTGGTG CGAGCCCTGC 1801 TGCAGAAGCC CAGTGCCCGC GTGGCTGTCC TGTTCACCCG TTCTGAGGAT GCCCGGGAGC 1861 TGCTTGCTGC CAGCCAGCGC CTCAATGCCA GCTTCACCTG GGTGGCCAGT GATGGTTGGG 1921 GGGCCCTGGA GAGTGTGGTG GCAGGCAGTG AGGGGGCTGC TGAGGGTGCT ATCACCATCG 1981 AGCTGGCCTC CTACCCCATC AGTGACTTTG CCTCCTACTT CCAGAGCCTG GACCCTTGGA 2041 ACAACAGCCG GAACCCCTGG TTCCGTGAAT TCTGGGAGCA GAGGTTCCGC TGCAGCTTCC 2101 GGCAGCGAGA CTGCGCAGCC CACTCTCTCC GGGCTGTGCC CTTTGAGCAG GAGTCCAAGA 2161 TCATGTTTGT GGTCAATGCA GTGTACGCCA TGGCCCATGC GCTCCACAAC ATGCACCGTG 2221 CCCTCTGCCC CAACACCACC CGGCTCTGTG ACGCGATGCG GCCAGTTAAC GGGCGCCGCC 2281 TCTACAAGGA CTTTGTGCTC AACGTCAAGT TTGATGCCCC CTTTCGCCCA GCTGACACCC 2341 ACAATGAGGT CCGCTTTGAC CGCTTTGGTG ATGGTATTGG CCGCTACAAC ATCTTCACCT 2401 ATCTGCGTGC AGGCAGTGGG CGCTATCGCT ACCAGAAGGT GGGCTACTGG GCAGAAGGCT 2461 TGACTCTGGA CACCAGCCTC ATCCCATGGG CCTCACCCTC AGCCGGCCCC CTGCCCGCCT 2521 CTCGCTGCAG TGAGCCCTGC CTCCAGAATG AGGTGAAGAG TGTGCAGCCG GGCGAAGTCT 2581 GCTGCTGGCT CTGCATTCCG TGCCAGCCCT ATGAGTACCG ATTGGACGAA TTCACTTGCG 2641 CTGATTGTGG CCTGGGCTAC TGGCCCAATG CCAGCCTGAC TGGCTGCTTC GAACTGCCCC 2701 AGGAGTACAT CCGCTGGGGC GATGCCTGGG CTGTGGGACC TGTCACCATC GCCTGCCTCG 2761 GTGCCCTGGC CACCCTCTTT GTGCTGGGTG TCTTTGTGCG GCACAATGCC ACACCAGTGG 2821 TCAAGGCCTC AGGTCGGGAG CTCTGCTACA TCCTGCTGGG TGGTGTCTTC CTCTGCTACT 2881 GCATGACCTT CATCTTCATT GCCAAGCCAT CCACGGCAGT GTGTACCTTA CGGCGTCTTG 2941 GTTTGGGCAC TGCCTTCTCT GTCTGCTACT CAGCCCTGCT CACCAAGACC AACCGCATTG 3001 CACGCATCTT CGGTGGGGCC CGGGAGGGTG CCCAGCGGCC ACGCTTCATC AGTCCTGCCT 3061 CACAGGTGGC CATCTGCCTG GCACTTATCT CGGGCCAGCT GCTCATCGTG GTCGCCTGGC 3121 TGGTGGTGGA GGCACCGGGC ACAGGCAAGG AGACAGCCCC CGAACGGCGG GAGGTGGTGA 3181 CACTGCGCTG CAACCACCGC GATGCAAGTA TGTTGGGCTC GCTGGCCTAC AATGTGCTCC 3241 TCATCGCGCT CTGCACGCTT TATGCCTTCA AGACTCGCAA GTGCCCCGAA AACTTCAACG 3301 AGGCCAAGTT CATTGGCTTC ACCATGTACA CCACCTGCAT CATCTGGCTG GCATTCCTGC 3361 CCATCTTCTA TGTCACCTCC AGTGACTACC GGGTACAGAC CACCACCATG TGCGTGTCAG 3421 TCAGCCTCAG CGGCTCCGTG GTGCTTGGCT GCCTCTTTGC GCCCAAGCTG CACATCATCC 3481 TCTTCCAGCC GCAGAAGAAC GTGGTTAGCC ACCGGGCACC CACCAGCCGC TTTGGCAGTG 3541 CTGCTGCCAG GGCCAGCTCC AGCCTTGGCC AAGGGTCTGG CTCCCAGTTT GTCCCCACTG 3601 TTTGCAATGG CCGTGAGGTG GTGGACTCGA CAACGTCATC GCTTACGCGG CCGCTCGAGC 3661 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 3721 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 3781 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 3841 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 3901 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 3961 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 4021 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 4081 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 4141 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 4201 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 4261 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 4321 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 4381 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 4441 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 4501 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 4561 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 4621 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 4681 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 4741 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 4801 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 4861 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 4921 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 4981 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 5041 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 5101 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 5161 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 5221 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 5281 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 5341 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 5401 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 5461 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 5521 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 5581 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 5641 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 5701 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 5761 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 5821 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 5881 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 5941 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 6001 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 6061 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 6121 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 6181 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 6241 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 6301 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 6361 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 6421 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 6481 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 6541 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 6601 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 6661 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 6721 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 6781 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 6841 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 6901 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 6961 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 7021 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 7081 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 7141 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 7201 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 7261 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 7321 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 7381 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 7441 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T //