LOCUS       RC218088                9066 bp    DNA     circular  SYN 
DEFINITION  LTBP1 (Myc-DDK-tagged)-Human latent transforming growth factor beta binding protein 1 (LTBP1), transcript variant 2
ACCESSION   NM_000627
VERSION     NM_000627.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5223..5314 
                     /label="Myc-DDK"  
     misc_feature    5344..5929 
                     /label="PolyA signal"  
     misc_feature    5401..5420 
                     /label="Restriction Enzyme"  
     misc_feature    6205..6877 
                     /label="ColE1"  
     misc_feature    7307..8101 
                     /label="Kan/Neo"  
     misc_feature    8136..8438 
                     /label="SV40 ori"  
     misc_feature    8524..9001 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5205..5211 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5205 
                     /label="LTBP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATACTAAG CTGATGTGTT TGTTGTTCTT TTTCTCCCTG CCTCCGCTCC 
     1081 TAGTGAGTAA CCACACTGGC CGCATCAAGG TGGTCTTTAC TCCGAGCATC TGTAAAGTGA 
     1141 CCTGCACCAA GGGCAGCTGT CAGAACAGCT GTGAGAAGGG GAACACCACC ACTCTCATTA 
     1201 GTGAGAATGG TCATGCTGCC GACACCCTGA CGGCCACGAA CTTCCGAGTG GTAATTTGCC 
     1261 ATCTTCCATG TATGAATGGT GGCCAGTGCA GTTCAAGGGA CAAATGTCAG TGCCCTCCAA 
     1321 ATTTCACAGG AAAACTTTGT CAGATCCCAG TCCATGGTGC CAGCGTGCCT AAACTTTATC 
     1381 AGCATTCCCA GCAGCCAGGC AAGGCGTTGG GGACGCATGT CATCCATTCA ACACATACCT 
     1441 TGCCTCTGAC CGTGACTAGC CAGCAAGGAG TCAAAGTGAA ATTTCCTCCT AACATAGTCA 
     1501 ATATCCATGT GAAACATCCT CCTGAAGCTT CCGTCCAGAT ACATCAGGTT TCAAGAATTG 
     1561 ATGGCCCAAC AGGCCAGAAG ACAAAAGAAG CTCAACCAGG CCAATCCCAA GTCTCGTACC 
     1621 AAGGGCTTCC TGTCCAGAAG ACCCAGACCA TACATTCCAC ATACTCCCAC CAGCAGGTCA 
     1681 TTCCTCACGT CTACCCCGTG GCTGCTAAGA CACAGCTTGG CCGGTGCTTC CAGGAAACCA 
     1741 TTGGGTCACA GTGTGGCAAA GCGCTCCCTG GCCTTTCAAA GCAAGAGGAC TGCTGTGGAA 
     1801 CTGTGGGTAC CTCCTGGGGC TTTAACAAAT GCCAGAAATG CCCCAAGAAA CCATCTTATC 
     1861 ATGGATACAA CCAAATGATG GAATGCCTAC CGGGTTATAA GCGGGTTAAC AACACCTTTT 
     1921 GCCAAGATAT TAATGAATGT CAGCTACAAG GTGTATGCCC TAATGGTGAG TGTTTGAATA 
     1981 CCATGGGCAG CTATCGATGT ACCTGCAAAA TAGGATTTGG GCCGGATCCT ACCTTTTCAA 
     2041 GTTGTGTTCC TGATCCCCCT GTGATCTCGG AAGAGAAAGG GCCCTGTTAC CGACTTGTCA 
     2101 GTTCTGGAAG ACAGTGTATG CACCCTCTGT CTGTTCACCT CACCAAGCAG CTCTGCTGTT 
     2161 GTAGTGTGGG CAAGGCCTGG GGCCCACACT GTGAGAAATG TCCCCTTCCA GGCACAGCTG 
     2221 CTTTTAAGGA AATCTGTCCT GGTGGAATGG GTTATACGGT TTCTGGCGTT CATAGACGCA 
     2281 GGCCAATCCA TCACCATGTA GGTAAAGGAC CTGTATTTGT CAAGCCAAAG AACACTCAAC 
     2341 CTGTTGCTAA AAGTACTCAT CCTCCACCTC TCCCAGCCAA GGAAGAGCCA GTGGAGGCCC 
     2401 TGACCTTCTC CCGGGAACAC GGGCCAGGAG TGGCGGAGCC AGAAGTGGCA ACTGCACCCC 
     2461 CTGAAAAGGA AATACCTTCA TTGGATCAAG AGAAAACCAA ACTTGAGCCT GGTCAACCCC 
     2521 AGCTGTCTCC AGGCATTTCC ACTATTCATC TGCATCCACA GTTTCCAGTA GTGATTGAAA 
     2581 AAACATCACC TCCTGTGCCT GTTGAAGTAG CTCCTGAAGC TTCTACGTCT AGTGCCAGCC 
     2641 AAGTGATTGC TCCTACTCAA GTGACAGAAA TCAATGAATG TACTGTGAAC CCTGATATCT 
     2701 GTGGAGCAGG ACACTGCATT AACCTACCAG TGAGATATAC CTGTATATGC TACGAGGGCT 
     2761 ACAGGTTCAG TGAACAACAG AGGAAATGTG TGGATATTGA TGAGTGTACT CAGGTCCAAC 
     2821 ACCTCTGCTC CCAGGGCCGC TGTGAAAACA CCGAGGGAAG TTTCTTGTGC ATTTGCCCAG 
     2881 CAGGATTTAT GGCCAGTGAG GAGGGTACTA ACTGCATAGA TGTTGACGAA TGCCTGAGGC 
     2941 CGGACGTCTG TGGGGAGGGG CACTGTGTCA ATACTGTGGG GGCCTTCCGG TGTGAATACT 
     3001 GTGACAGCGG GTACCGCATG ACTCAGAGAG GCCGTTGTGA GGATATTGAT GAATGTTTGA 
     3061 ATCCAAGCAC TTGTCCAGAT GAGCAGTGTG TGAATTCTCC TGGATCTTAC CAGTGCGTTC 
     3121 CCTGCACAGA AGGATTCCGA GGCTGGAATG GACAGTGCCT TGATGTGGAC GAGTGCCTGG 
     3181 AACCAAACGT CTGCGCAAAT GGTGATTGTT CCAACCTTGA AGGCTCCTAC ATGTGTTCAT 
     3241 GCCACAAAGG CTATACCCGG ACTCCGGACC ACAAGCACTG TAGAGATATT GATGAATGTC 
     3301 AGCAAGGGAA TCTATGTGTA AACGGGCAGT GCAAAAATAC CGAGGGCTCC TTCAGGTGCA 
     3361 CCTGTGGACA GGGGTACCAG CTGTCGGCAG CTAAAGACCA GTGTGAAGAC ATTGATGAAT 
     3421 GCCAGCACCG TCATCTCTGT GCTCATGGGC AGTGCAGGAA CACTGAGGGC TCTTTTCAAT 
     3481 GTGTGTGTGA CCAGGGTTAC AGAGCATCTG GGCTTGGAGA CCACTGTGAA GATATCAATG 
     3541 AATGCTTGGA GGACAAGAGT GTTTGCCAGA GAGGAGACTG CATTAATACT GCAGGGTCCT 
     3601 ATGATTGTAC TTGTCCGGAT GGATTTCAGC TAGATGACAA TAAAACATGT CAAGATATTA 
     3661 ATGAATGTGA ACATCCAGGG CTCTGTGGTC CGCAAGGGGA GTGCCTAAAC ACAGAGGGTT 
     3721 CTTTCCATTG TGTCTGCCAG CAGGGTTTCT CAATCTCTGC AGATGGCCGT ACGTGTGAAG 
     3781 ATATTGATGA ATGTGTAAAC AACACTGTTT GTGACAGTCA CGGGTTTTGT GACAATACAG 
     3841 CTGGCTCCTT CCGCTGCCTC TGTTATCAGG GCTTTCAAGC CCCACAGGAT GGGCAAGGGT 
     3901 GTGTGGATGT GAATGAATGT GAACTGCTCA GTGGGGTGTG TGGTGAAGCC TTCTGTGAAA 
     3961 ACGTGGAAGG GTCCTTCCTG TGCGTGTGTG CTGATGAAAA CCAAGAGTAC AGCCCCATGA 
     4021 CTGGGCAGTG CCGCTCCCGG ACCTCCACAG ATTTAGATGT AGATGTAGAT CAACCCAAAG 
     4081 AAGAAAAGAA AGAATGCTAC TATAATCTCA ATGACGCCAG TCTCTGTGAT AATGTGTTGG 
     4141 CCCCCAATGT CACGAAACAA GAATGCTGCT GTACATCAGG CGCGGGATGG GGAGATAACT 
     4201 GCGAAATCTT CCCCTGCCCG GTCTTGGGAA CTGCTGAGTT CACTGAAATG TGTCCCAAAG 
     4261 GGAAAGGTTT TGTGCCTGCT GGAGAATCAT CTTCTGAAGC TGGTGGTGAG AACTATAAAG 
     4321 ATGCAGATGA ATGCCTACTT TTTGGACAAG AAATCTGCAA AAATGGTTTC TGTTTGAACA 
     4381 CTCGGCCTGG GTATGAATGC TACTGTAAGC AAGGGACGTA CTATGATCCT GTGAAACTGC 
     4441 AGTGCTTTGA TATGGATGAA TGTCAAGACC CCAGTAGTTG TATTGATGGC CAGTGTGTTA 
     4501 ATACAGAGGG CTCTTACAAC TGCTTCTGTA CTCACCCCAT GGTCCTGGAT GCGTCAGAAA 
     4561 AAAGATGTAT ACGACCGGCT GAGTCAAACG AACAAATAGA AGAAACTGAT GTCTACCAAG 
     4621 ATTTGTGCTG GGAACATCTG AGTGATGAAT ACGTGTGTAG CCGGCCTCTT GTGGGCAAGC 
     4681 AGACAACGTA CACTGAGTGC TGCTGTCTGT ATGGAGAGGC CTGGGGCATG CAGTGTGCCC 
     4741 TCTGCCCCCT GAAGGATTCA GATGACTATG CTCAGCTGTG TAACATCCCC GTGACGGGAC 
     4801 GCCGGCAGCC ATATGGACGG GACGCCTTGG TTGACTTCAG TGAACAGTAT ACTCCAGAAG 
     4861 CCGATCCCTA CTTCATCCAA GACCGTTTTC TAAATAGCTT TGAGGAGTTA CAGGCTGAGG 
     4921 AATGCGGCAT CCTCAATGGA TGTGAAAATG GTCGCTGTGT GAGGGTCCAG GAAGGTTACA 
     4981 CCTGCGATTG CTTTGATGGG TATCACTTGG ATACGGCCAA GATGACCTGT GTCGATGTAA 
     5041 ATGAATGCGA TGAGTTGAAC AACCGGATGT CTCTCTGCAA GAATGCCAAG TGCATTAACA 
     5101 CCGATGGTTC CTACAAGTGT TTGTGTCTGC CAGGCTACGT GCCTTCTGAC AAGCCAAACT 
     5161 ACTGCACTCC GTTGAATACC GCCTTGAATT TAGAGAAAGA CAGTGACCTG GAGACGCGTA 
     5221 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5281 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5341 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5401 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5461 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5521 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5581 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5641 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5701 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5761 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5821 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5881 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5941 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6001 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6061 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6121 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6181 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6241 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6301 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6361 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6421 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6481 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6541 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6601 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6661 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6721 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6781 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6841 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6901 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6961 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7021 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7081 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7141 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7201 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7261 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7321 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7381 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7441 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7501 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7561 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7621 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7681 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7741 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7801 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7861 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7921 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7981 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8041 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8101 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8161 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8221 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8281 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8341 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8401 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8461 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8521 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8581 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8641 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8701 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8761 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8821 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8881 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8941 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9001 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9061 AATTTT
//