LOCUS       RC218040                8448 bp    DNA     circular  SYN 
DEFINITION  INPP5D (Myc-DDK-tagged)-Human inositol polyphosphate-5-phosphatase, 145kDa (INPP5D), transcript variant 1
ACCESSION   NM_001017915
VERSION     NM_001017915.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4605..4696 
                     /label="Myc-DDK"  
     misc_feature    4726..5311 
                     /label="PolyA signal"  
     misc_feature    4783..4802 
                     /label="Restriction Enzyme"  
     misc_feature    5587..6259 
                     /label="ColE1"  
     misc_feature    6689..7483 
                     /label="Kan/Neo"  
     misc_feature    7518..7820 
                     /label="SV40 ori"  
     misc_feature    7906..8383 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4587..4593 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4587 
                     /label="INPP5D"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCCCTGC TGGAACCATG GCAACATCAC CCGCTCCAAG GCGGAGGAGC 
     1081 TGCTTTCCAG GACAGGCAAG GACGGGAGCT TCCTCGTGCG TGCCAGCGAG TCCATCTCCC 
     1141 GGGCATACGC GCTCTGCGTG CTGTATCGGA ATTGCGTTTA CACTTACAGA ATTCTGCCCA 
     1201 ATGAAGATGA TAAATTCACT GTTCAGGCAT CCGAAGGCGT CTCCATGAGG TTCTTCACCA 
     1261 AGCTGGACCA GCTCATCGAG TTTTACAAGA AGGAAAACAT GGGGCTGGTG ACCCATCTGC 
     1321 AATACCCTGT GCCGCTGGAG GAAGAGGACA CAGGCGACGA CCCTGAGGAG GACACAGTAG 
     1381 AAAGTGTCGT GTCTCCACCC GAGCTGCCCC CAAGAAACAT CCCGCTGACT GCCAGCTCCT 
     1441 GTGAGGCCAA GGAGGTTCCT TTTTCAAACG AGAATCCCCG AGCGACCGAG ACCAGCCGGC 
     1501 CGAGCCTCTC CGAGACATTG TTCCAGCGAC TGCAAAGCAT GGACACCAGT GGGCTTCCAG 
     1561 AAGAGCATCT TAAGGCCATC CAAGATTATT TAAGCACTCA GCTCGCCCAG GACTCTGAAT 
     1621 TTGTGAAGAC AGGGTCCAGC AGTCTTCCTC ACCTGAAGAA ACTGACCACA CTGCTCTGCA 
     1681 AGGAGCTCTA TGGAGAAGTC ATCCGGACCC TCCCATCCCT GGAGTCTCTG CAGAGGTTAT 
     1741 TTGACCAGCA GCTCTCCCCG GGCCTCCGTC CACGTCCTCA GGTTCCTGGT GAGGCCAATC 
     1801 CCATCAACAT GGTGTCCAAG CTCAGCCAAC TGACAAGCCT GTTGTCATCC ATTGAAGACA 
     1861 AGGTCAAGGC CTTGCTGCAC GAGGGTCCTG AGTCTCCGCA CCGGCCCTCC CTTATCCCTC 
     1921 CAGTCACCTT TGAGGTGAAG GCAGAGTCTC TGGGGATTCC TCAGAAAATG CAGCTCAAAG 
     1981 TCGACGTTGA GTCTGGGAAA CTGATCATTA AGAAGTCCAA GGATGGTTCT GAGGACAAGT 
     2041 TCTACAGCCA CAAGAAAATC CTGCAGCTCA TTAAGTCACA GAAATTTCTG AATAAGTTGG 
     2101 TGATCTTGGT GGAAACAGAG AAGGAGAAGA TCCTGCGGAA GGAATATGTT TTTGCTGACT 
     2161 CCAAAAAGAG AGAAGGCTTC TGCCAGCTCC TGCAGCAGAT GAAGAACAAG CACTCAGAGC 
     2221 AGCCGGAGCC CGACATGATC ACCATCTTCA TCGGCACCTG GAACATGGGT AACGCCCCCC 
     2281 CTCCCAAGAA GATCACGTCC TGGTTTCTCT CCAAGGGGCA GGGAAAGACG CGGGACGACT 
     2341 CTGCGGACTA CATCCCCCAT GACATTTACG TGATCGGCAC CCAAGAGGAC CCCCTGAGTG 
     2401 AGAAGGAGTG GCTGGAGATC CTCAAACACT CCCTGCAAGA AATCACCAGT GTGACTTTTA 
     2461 AAACAGTCGC CATCCACACG CTCTGGAACA TCCGCATCGT GGTGCTGGCC AAGCCTGAGC 
     2521 ACGAGAACCG GATCAGCCAC ATCTGTACTG ACAACGTGAA GACAGGCATT GCAAACACAC 
     2581 TGGGGAACAA GGGAGCCGTG GGGGTGTCGT TCATGTTCAA TGGAACCTCC TTAGGGTTCG 
     2641 TCAACAGCCA CTTGACTTCA GGAAGTGAAA AGAAACTCAG GCGAAACCAA AACTATATGA 
     2701 ACATTCTCCG GTTCCTGGCC CTGGGCGACA AGAAGCTGAG TCCCTTTAAC ATCACTCACC 
     2761 GCTTCACGCA CCTCTTCTGG TTTGGGGATC TTAACTACCG TGTGGATCTG CCTACCTGGG 
     2821 AGGCAGAAAC CATCATCCAG AAAATCAAGC AGCAGCAGTA CGCAGACCTC CTGTCCCACG 
     2881 ACCAGCTGCT CACAGAGAGG AGGGAGCAGA AGGTCTTCCT ACACTTCGAG GAGGAAGAAA 
     2941 TCACGTTTGC CCCAACCTAC CGTTTTGAGA GACTGACTCG GGACAAATAC GCCTACACCA 
     3001 AGCAGAAAGC GACAGGGATG AAGTACAACT TGCCTTCCTG GTGTGACCGA GTCCTCTGGA 
     3061 AGTCTTATCC CCTGGTGCAC GTGGTGTGTC AGTCTTATGG CAGTACCAGC GACATCATGA 
     3121 CGAGTGACCA CAGCCCTGTC TTTGCCACAT TTGAGGCAGG AGTCACTTCC CAGTTTGTCT 
     3181 CCAAGAACGG TCCCGGGACT GTTGACAGCC AAGGACAGAT TGAGTTTCTC AGGTGCTATG 
     3241 CCACATTGAA GACCAAGTCC CAGACCAAAT TCTACCTGGA GTTCCACTCG AGCTGCTTGG 
     3301 AGAGTTTTGT CAAGAGTCAG GAAGGAGAAA ATGAAGAAGG AAGTGAGGGG GAGCTGGTGG 
     3361 TGAAGTTTGG TGAGACTCTT CCAAAGCTGA AGCCCATTAT CTCTGACCCT GAGTACCTGC 
     3421 TAGACCAGCA CATCCTCATC AGCATCAAGT CCTCTGACAG CGACGAATCC TATGGCGAGG 
     3481 GCTGCATTGC CCTTCGGTTA GAGGCCACAG AAACGCAGCT GCCCATCTAC ACGCCTCTCA 
     3541 CCCACCATGG GGAGTTGACA GGCCACTTCC AGGGGGAGAT CAAGCTGCAG ACCTCTCAGG 
     3601 GCAAGACGAG GGAGAAGCTC TATGACTTTG TGAAGACGGA GCGTGATGAA TCCAGTGGGC 
     3661 CAAAGACCCT GAAGAGCCTC ACCAGCCACG ACCCCATGAA GCAGTGGGAA GTCACTAGCA 
     3721 GGGCCCCTCC GTGCAGTGGC TCCAGCATCA CTGAAATCAT CAACCCCAAC TACATGGGAG 
     3781 TGGGGCCCTT TGGGCCACCA ATGCCCCTGC ACGTGAAGCA GACCTTGTCC CCTGACCAGC 
     3841 AGCCCACAGC CTGGAGCTAC GACCAGCCGC CCAAGGACTC CCCGCTGGGG CCCTGCAGGG 
     3901 GAGAAAGTCC TCCGACACCT CCCGGCCAGC CGCCCATATC ACCCAAGAAG TTTTTACCCT 
     3961 CAACAGCAAA CCGGGGTCTC CCTCCCAGGA CACAGGAGTC AAGGCCCAGT GACCTGGGGA 
     4021 AGAACGCAGG GGACACGCTG CCTCAGGAGG ACCTGCCGCT GACGAAGCCC GAGATGTTTG 
     4081 AGAACCCCCT GTATGGGTCC CTGAGTTCCT TCCCTAAGCC TGCTCCCAGG AAGGACCAGG 
     4141 AATCCCCCAA AATGCCGCGG AAGGAACCCC CGCCCTGCCC GGAACCCGGC ATCTTGTCGC 
     4201 CCAGCATCGT GCTCACCAAA GCCCAGGAGG CTGATCGCGG CGAGGGGCCC GGCAAGCAGG 
     4261 TGCCCGCGCC CCGGCTGCGC TCCTTCACGT GCTCATCCTC TGCCGAGGGC AGGGCGGCCG 
     4321 GCGGGGACAA GAGCCAAGGG AAGCCCAAGA CCCCGGTCAG CTCCCAGGCC CCGGTGCCGG 
     4381 CCAAGAGGCC CATCAAGCCT TCCAGATCGG AAATCAACCA GCAGACCCCG CCCACCCCGA 
     4441 CGCCGCGGCC GCCGCTGCCA GTCAAGAGCC CGGCGGTGCT GCACCTCCAG CACTCCAAGG 
     4501 GCCGCGACTA CCGCGACAAC ACCGAGCTCC CGCATCACGG CAAGCACCGG CCGGAGGAGG 
     4561 GGCCACCAGG GCCTCTAGGC AGGACTGCCA TGCAGACGCG TACGCGGCCG CTCGAGCAGA 
     4621 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4681 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4741 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4801 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4861 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4921 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4981 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     5041 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     5101 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     5161 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     5221 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5281 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5341 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5401 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5461 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5521 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5581 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5641 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5701 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5761 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5821 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5881 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5941 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     6001 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     6061 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     6121 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     6181 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6241 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6301 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6361 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6421 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6481 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6541 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6601 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6661 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6721 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6781 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6841 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6901 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6961 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     7021 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     7081 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     7141 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     7201 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7261 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7321 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7381 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7441 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7501 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7561 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7621 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7681 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7801 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7861 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7921 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7981 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     8041 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     8101 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     8161 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     8221 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8281 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8341 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8401 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//