LOCUS       RC218019                9033 bp    DNA     circular  SYN 
DEFINITION  CNTNAP1 (Myc-DDK-tagged)-Human contactin associated protein 1 (CNTNAP1)
ACCESSION   NM_003632
VERSION     NM_003632.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5190..5281 
                     /label="Myc-DDK"  
     misc_feature    5311..5896 
                     /label="PolyA signal"  
     misc_feature    5368..5387 
                     /label="Restriction Enzyme"  
     misc_feature    6172..6844 
                     /label="ColE1"  
     misc_feature    7274..8068 
                     /label="Kan/Neo"  
     misc_feature    8103..8405 
                     /label="SV40 ori"  
     misc_feature    8491..8968 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5172..5178 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5172 
                     /label="CNTNAP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCATCTC CGGCTCTTCT GCATCCTGCT CGCCGCGGTC TCAGGAGCCG 
     1081 AGGGCTGGGG CTACTACGGC TGCGACGAGG AGCTGGTGGG TCCCCTGTAT GCACGCTCCC 
     1141 TGGGCGCCTC CTCCTACTAC AGTCTCCTTA CTGCGCCGCG ATTCGCCAGG CTGCACGGCA 
     1201 TAAGCGGGTG GTCACCACGG ATTGGGGATC CGAATCCCTG GCTCCAGATA GACTTAATGA 
     1261 AGAAGCACCG GATCCGGGCC GTGGCCACAC AGGGCTCCTT TAATTCTTGG GACTGGGTCA 
     1321 CACGTTACAT GCTACTCTAC GGCGACCGAG TGGACAGCTG GACACCGTTC TACCAGCGAG 
     1381 GGCACAACTC GACCTTCTTT GGTAACGTGA ACGAGTCGGC GGTGGTGCGC CATGACCTGC 
     1441 ACTTCCACTT CACTGCGCGC TACATCCGCA TCGTGCCCCT GGCCTGGAAC CCACGCGGCA 
     1501 AGATCGGCCT GAGGCTCGGC CTCTATGGCT GCCCATACAA GGCCGACATA CTCTATTTCG 
     1561 ACGGCGACGA TGCCATCTCC TACCGCTTCC CGCGAGGGGT CAGCCGAAGC CTGTGGGACG 
     1621 TGTTCGCCTT CAGCTTCAAG ACCGAGGAGA AGGACGGTCT TCTGCTGCAC GCCGAGGGCG 
     1681 CCCAGGGCGA CTACGTGACG CTCGAGCTGG AGGGGGCACA CCTGCTGCTG CACATGAGCC 
     1741 TGGGCAGCAG CCCTATCCAG CCAAGACCAG GTCACACCAC CGTGAGCGCA GGCGGAGTCC 
     1801 TCAATGACCA GCACTGGCAC TATGTGCGGG TGGACCGATT TGGCCGCGAT GTAAATTTCA 
     1861 CCCTGGACGG CTATGTGCAG CGCTTTATTC TCAATGGAGA CTTCGAGAGG CTGAACCTGG 
     1921 ACACTGAGAT GTTCATCGGA GGTCTGGTGG GCGCCGCGCG GAAGAACCTG GCCTATCGGC 
     1981 ATAACTTCCG CGGCTGCATA GAAAACGTAA TCTTCAACCG CGTCAACATC GCAGACCTGG 
     2041 CCGTGCGGCG CCATTCCCGG ATCACCTTCG AGGGTAAGGT GGCTTTTCGT TGCCTGGACC 
     2101 CGGTACCGCA CCCTATCAAC TTCGGAGGCC CTCACAACTT CGTTCAAGTG CCCGGTTTCC 
     2161 CACGCCGTGG CCGCCTGGCA GTCTCATTTC GCTTCCGCAC CTGGGACCTC ACCGGGCTTC 
     2221 TCCTTTTCTC CCGTCTGGGG GACGGGCTGG GCCACGTGGA GCTGACGCTC AGCGAAGGGC 
     2281 AGGTCAACGT GTCCATCGCG CAGAGCGGCC GAAAGAAGCT TCAGTTCGCT GCTGGGTACC 
     2341 GACTGAATGA CGGCTTTTGG CACGAGGTGA ATTTTGTGGC ACAGGAAAAC CATGCAGTTA 
     2401 TCAGCATTGA TGATGTGGAA GGGGCAGAGG TCAGGGTCTC ATACCCGTTG CTGATCCGGA 
     2461 CAGGGACCTC ATATTTCTTT GGGGGTTGTC CCAAGCCAGC CAGTCGATGG GACTGCCACT 
     2521 CCAACCAGAC GGCATTCCAT GGCTGCATGG AGCTGCTCAA GGTGGATGGT CAACTGGTCA 
     2581 ACCTGACTCT GGTGGAGGGC CGGCGGCTTG GATTCTATGC TGAGGTCCTC TTTGATACAT 
     2641 GTGGCATCAC TGATAGGTGC AGCCCTAACA TGTGTGAGCA TGATGGACGC TGCTACCAGT 
     2701 CTTGGGATGA CTTCATTTGC TACTGCGAAC TGACGGGCTA CAAGGGAGAG ACCTGCCACA 
     2761 CACCTTTGTA TAAGGAATCC TGTGAGGCTT ATCGGCTCAG TGGGAAAACT TCTGGAAACT 
     2821 TCACCATTGA TCCTGATGGC AGTGGCCCCC TGAAGCCATT TGTAGTGTAC TGTGATATCC 
     2881 GAGAGAACCG AGCGTGGACA GTTGTGCGGC ATGACAGGCT GTGGACAACT CGAGTGACAG 
     2941 GTTCCAGCAT GGAGCGGCCA TTCCTGGGGG CTATCCAGTA CTGGAATGCA TCCTGGGAGG 
     3001 AAGTCAGTGC CCTTGCCAAT GCTTCCCAGC ATTGTGAACA GTGGATCGAG TTCTCCTGCT 
     3061 ACAATTCCCG GCTGCTCAAC ACTGCAGGAG GCTACCCCTA CAGCTTTTGG ATTGGCCGAA 
     3121 ATGAGGAGCA GCACTTCTAC TGGGGAGGCT CCCAGCCTGG GATCCAGCGC TGTGCCTGTG 
     3181 GTCTGGACCG GAGCTGTGTG GACCCTGCCT TGTACTGCAA CTGTGACGCT GACCAGCCCC 
     3241 AGTGGAGAAC TGACAAGGGA CTGCTGACCT TTGTGGACCA TCTGCCTGTC ACTCAGGTAG 
     3301 TGATAGGGGA TACGAACCGC TCCACTTCTG AGGCCCAGTT CTTCCTGAGG CCTCTGCGCT 
     3361 GCTATGGCGA TCGAAATTCC TGGAACACCA TTTCCTTCCA CACCGGGGCT GCACTACGCT 
     3421 TCCCCCCAAT CCGTGCCAAC CACAGCCTGG ATGTCTCCTT CTACTTCAGG ACCTCTGCTC 
     3481 CCTCGGGGGT CTTCCTAGAG AATATGGGGG GCCCTTACTG CCAGTGGCGC CGACCTTATG 
     3541 TGCGGGTGGA ACTCAACACA TCCCGGGATG TGGTCTTCGC CTTTGATGTG GGGAATGGGG 
     3601 ATGAGAACCT CACAGTACAC TCAGACGACT TTGAGTTCAA TGATGACGAG TGGCACCTGG 
     3661 TCCGGGCTGA AATCAACGTG AAGCAGGCCC GGCTCCGAGT GGATCACCGG CCCTGGGTTC 
     3721 TGCGGCCTAT GCCACTGCAG ACCTACATCT GGATGGAGTA TGACCAGCCC CTCTATGTGG 
     3781 GATCTGCAGA GCTTAAGAGA CGCCCCTTTG TGGGTTGCTT GAGGGCCATG CGTCTGAACG 
     3841 GAGTGACTCT GAACCTGGAG GGCCGTGCCA ATGCCTCTGA GGGTACCTCA CCCAACTGCA 
     3901 CAGGCCACTG TGCCCACCCT CGGCTCCCCT GTTTCCATGG AGGCCGCTGC GTGGAGCGCT 
     3961 ATAGCTACTA CACGTGTGAC TGTGACCTCA CGGCTTTTGA TGGGCCATAC TGCAACCACG 
     4021 ATATTGGTGG TTTCTTTGAG CCGGGCACCT GGATGCGCTA TAACCTACAG TCAGCGCTGC 
     4081 GCTCTGCAGC CAGGGAGTTC TCCCACATGC TGAGCCGGCC AGTGCCAGGC TATGAGCCTG 
     4141 GCTACATCCC GGGCTATGAT ACTCCGGGCT ATGTGCCTGG CTACCATGGC CCCGGGTACC 
     4201 GCCTGCCCGA CTACCCCCGG CCTGGTCGGC CTGTGCCCGG TTACCGTGGG CCTGTCTACA 
     4261 ACGTTACGGG AGAGGAGGTC TCCTTCAGCT TCAGCACCAG CTCCGCCCCT GCTGTCCTGC 
     4321 TCTACGTCAG TTCCTTTGTT CGTGACTACA TGGCTGTGCT CATCAAGGAT GATGGGACCC 
     4381 TTCAGCTGCG ATATCAGCTG GGCACCAGTC CCTACGTGTA CCAGCTAACC ACTCGACCAG 
     4441 TGACCGATGG CCAGCCCCAT AGCATCAATA TCACCCGTGT TTACCGGAAC CTCTTCATCC 
     4501 AGGTGGACTA CTTCCCACTG ACAGAGCAGA AGTTCTCGCT GTTGGTGGAC AGCCAGTTGG 
     4561 ACTCACCCAA GGCCTTGTAT TTAGGGCGTG TGATGGAGAC AGGAGTCATT GACCCGGAGA 
     4621 TCCAGCGCTA CAACACCCCA GGTTTCTCAG GCTGCCTGTC TGGTGTTCGA TTCAACAACG 
     4681 TGGCTCCCCT CAAGACCCAC TTCCGAACCC CTCGACCCAT GACTGCTGAG CTAGCTGAGG 
     4741 CCCTTCGAGT TCAGGGAGAA CTGTCCGAAT CTAATTGCGG AGCTATGCCA CGTCTTGTTT 
     4801 CAGAGGTGCC ACCTGAGCTT GATCCCTGGT ATCTGCCCCC AGACTTCCCC TACTACCATG 
     4861 ATGAAGGATG GGTTGCCATA CTTTTAGGCT TTTTGGTGGC CTTTCTGCTG CTGGGGCTGG 
     4921 TGGGAATGTT GGTGCTCTTC TATCTGCAAA ATCATCGCTA TAAGGGCTCC TACCATACCA 
     4981 ATGAGCCCAA GGCTGCCCAC GAGTACCATC CTGGCAGCAA ACCTCCCCTA CCCACTTCAG 
     5041 GCCCTGCCCA GGTCCCCACC CCTACAGCAG CTCCCAACCA AGCTCCAGCC TCAGCCCCAG 
     5101 CCCCAGCCCC AACTCCAGCC CCAGCCCCTG GCCCCCGGGA TCAGAACCTA CCCCAGATCC 
     5161 TGGAGGAGTC CAGGTCTGAA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5221 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5281 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5341 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5401 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5461 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5521 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5581 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5641 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5701 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5761 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5821 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5881 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5941 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6001 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6061 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6121 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6181 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6241 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6301 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6361 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6421 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6481 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6541 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6601 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6661 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6721 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6781 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6841 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6901 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6961 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7021 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7081 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7141 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7201 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7261 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7321 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7381 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7441 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7501 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7561 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7621 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7681 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7741 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7801 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7861 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7921 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7981 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8041 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8101 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8161 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8221 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8281 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8341 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8401 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8461 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8521 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8581 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8641 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8701 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8761 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8821 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8881 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8941 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9001 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//