LOCUS       RC218001                8058 bp    DNA     circular  SYN 
DEFINITION  ACAD10 (Myc-DDK-tagged)-Human acyl-CoA dehydrogenase family, member 10 (ACAD10), transcript variant 2
ACCESSION   NM_025247
VERSION     NM_025247.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4215..4306 
                     /label="Myc-DDK"  
     misc_feature    4336..4921 
                     /label="PolyA signal"  
     misc_feature    4393..4412 
                     /label="Restriction Enzyme"  
     misc_feature    5197..5869 
                     /label="ColE1"  
     misc_feature    6299..7093 
                     /label="Kan/Neo"  
     misc_feature    7128..7430 
                     /label="SV40 ori"  
     misc_feature    7516..7993 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4197..4203 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4197 
                     /label="ACAD10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGTGTCAGG AGCTGTTTCC AGTCCCCCCG TCTCCAGTGG GTGTGGAGAA 
     1081 CAGCCTTCCT GAAACACACC CAGCGCAGGC ACCAGGGGTC CCACCGATGG ACACACCTTG 
     1141 GAGGCAGCAC CTACAGAGCG GTGATTTTCG ACATGGGCGG AGTTCTCATT CCTTCTCCAG 
     1201 GGAGAGTCGC TGCAGAATGG GAGGTACAGA ATCGTATCCC TTCTGGAACT ATATTAAAGG 
     1261 CCTTGATGGA AGGTGGTGAA AATGGGCCCT GGATGAGATT TATGAGAGCA GAAATAACAG 
     1321 CAGAGGGTTT TTTACGAGAA TTTGGGAGAC TTTGCTCTGA AATGTTAAAG ACCTCCGTGC 
     1381 CTGTGGACTC ATTTTTCTCT CTGTTGACCA GTGAGCGAGT GGCAAAGCAG TTCCCAGTGA 
     1441 TGACTGAGGC CATAACTCAA ATTCGGGCAA AAGGTCTTCA GACTGCAGTC TTGAGCAATA 
     1501 ATTTTTATCT TCCCAACCAG AAAAGCTTTT TGCCCCTGGA CCGGAAACAG TTTGATGTGA 
     1561 TTGTGGAGTC CTGCATGGAA GGGATCTGTA AGCCAGACCC TAGGATCTAC AAGCTGTGCT 
     1621 TGGAGCAGCT CGGCCTGCAG CCCTCTGAGT CCATCTTTCT TGATGACCTT GGAACAAATC 
     1681 TAAAAGAAGC TGCCAGACTT GGTATTCACA CCATTAAGGT TAATGACCCA GAGACTGCAG 
     1741 TAAAGGAATT AGAAGCTCTC TTGGGTTTTA CATTGAGAGT AGGTGTTCCA AACACTCGGC 
     1801 CTGTGAAAAA GACGATGGAA ATTCCGAAAG ATTCCTTGCA GAAGTACCTC AAAGACTTAC 
     1861 TGGGTATCCA GACCACAGGC CCATTGGAAC TACTTCAGTT TGATCACGGG CAGTCAAATC 
     1921 CAACTTACTA CATCAGGCTG GCTAATCGTG ATCTAGTTCT GAGGAAGAAG CCCCCAGGGA 
     1981 CACTCCTTCC ATCTGCCCAT GCCATAGAGA GGGAGTTCAG GATTATGAAA GCCCTTGCAA 
     2041 ATGCTGGAGT ACCTGTCCCT AACGTTCTTG ATCTCTGTGA AGATTCAAGT GTCATTGGCA 
     2101 CCCCCTTCTA TGTGATGGAG TACTGCCCAG GTCTCATCTA CAAAGACCCT TCCCTGCCAG 
     2161 GCTTGGAGCC CAGCCACAGA CGAGCCATAT ACACTGCCAT GAACACAGTC CTGTGCAAAA 
     2221 TTCACAGTGT GGATCTGCAG GCTGTGGGAC TTGAAGACTA TGGGAAGCAA GGGGACTATA 
     2281 TTCCACGCCA GGTACGAACC TGGGTTAAGC AGTATCGAGC TTCCGAAACT AGCACCATCC 
     2341 CAGCCATGGA GAGGCTGATC GAATGGCTGC CCCTCCATCT TCCCCGTCAG CAGAGGACCA 
     2401 CAGTGGTGCA CGGGGACTTC AGGCTCGACA ACCTGGTGTT TCATCCAGAA GAGCCAGAGG 
     2461 TGCTTGCTGT CCTTGACTGG GAACTTTCTA CCTTGGGCGA CCCCCTTGCT GATGTGGCCT 
     2521 ACAGCTGCCT GGCTCATTAC CTGCCATCCA GTTTTCCCGT GCTGAGAGGT ATTAATGACT 
     2581 GTGACTTGAC ACAGCTGGGA ATCCCTGCTG CAGAGGAGTA TTTCAGGATG TACTGTCTCC 
     2641 AAATGGGGCT CCCTCCCACT GAGAACTGGA ACTTCTATAT GGCTTTTTCC TTTTTCCGTG 
     2701 TGGCTGCAAT CCTACAGGGA GTCTACAAGC GATCACTCAC AGGGCAAGCA AGCTCCACAT 
     2761 ATGCGGAACA AACTGGAAAG CTGACCGAAT TTGTGTCTAA CCTGGCGTGG GATTTCGCAG 
     2821 TCAAAGAAGG GTTCCGGGTT TTCAAAGAGA TGCCCTTCAC AAATCCGTTA ACAAGGTCCT 
     2881 ACCACACGTG GGCCAGGCCC CAGTCCCAGT GGTGCCCCAC AGGCAGCAGG AGTTATAGCT 
     2941 CCGTTCCAGA AGCTTCCCCA GCTCATACCT CAAGGGGAGG TCTGGTTATC TCTCCAGAGA 
     3001 GCCTCTCTCC ACCTGTCAGA GAGCTGTATC ACCGGCTGAA GCACTTCATG GAGCAACGTG 
     3061 TGTACCCTGC AGAGCCAGAG CTGCAGAGTC ACCAGGCCTC AGCAGCCAGG TGGAGCCCCT 
     3121 CCCCACTGAT CGAAGACCTC AAGGAGAAAG CCAAAGCTGA AGGACTTTGG AACCTTTTCC 
     3181 TACCCTTAGA GGCTGATCCC GAGAAAAAAT ACGGAGCAGG ACTGACCAAT GTGGAATATG 
     3241 CACATCTGTG TGAGCTCATG GGCACGTCCC TGTATGCCCC CGAGGTATGT AACTGCTCTG 
     3301 CGCCTGACAC GGGCAACATG GAGCTGCTGG TGAGGTATGG CACCGAAGCG CAGAAGGCTC 
     3361 GCTGGCTGAT TCCTCTGCTG GAGGGGAAAG CCCGCTCCTG TTTTGCTATG ACCGAGCCCC 
     3421 AGGTTGCCTC TTCAGATGCC ACCAACATTG AGGCTTCCAT CAGAGAGGAG GACAGCTTCT 
     3481 ATGTCATAAA CGGTCACAAA TGGTGGATCA CAGGCATCCT GGATCCTCGT TGCCAACTCT 
     3541 GTGTGTTTAT GGGAAAAACA GACCCACATG CACCAAGACA CCGGCAGCAG TCTGTGCTCT 
     3601 TGGTTCCCAT GGATACCCCA GGGATAAAAA TCATCCGGCC TCTGACGGTG TATGGACTGG 
     3661 AAGATGCACC AGGTGGCCAT GGTGAAGTCC GATTTGAGCA CGTGCGTGTG CCCAAAGAGA 
     3721 ACATGGTCCT GGGCCCTGGC CGAGGCTTTG AGATCGCCCA GGGCAGACTG GGCCCCGGCA 
     3781 GGATCCATCA CTGCATGAGG CTGATCGGGT TCTCAGAGAG GGCCCTGGCA CTCATGAAGG 
     3841 CCCGCGTGAA GTCCCGCTTG GCTTTTGGGA AGCCCCTGGT GGAGCAGGGC ACAGTGCTGG 
     3901 CGGACATCGC GCAGTCGCGC GTGGAGATTG AGCAGGCACG GCTGCTGGTG CTGAGAGCTG 
     3961 CCCACCTCAT GGACCTGGCA GGAAACAAGG CTGCAGCCTT GGATATAGCC ATGATTAAAA 
     4021 TGGTCGCCCC GTCCATGGCC TCCCGAGTGA TTGATCGTGC GATTCAGGCC TTTGGAGCAG 
     4081 CAGGCCTGAG CAGCGACTAC CCACTGGCTC AGTTCTTCAC CTGGGCCCGA GCCCTGCGCT 
     4141 TTGCCGACGG CCCTGACGAG GTGCACCGGG CCACGGTGGC CAAGCTAGAG CTGAAGCACC 
     4201 GCATTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4261 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4321 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4381 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4441 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4501 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4561 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4621 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4681 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4741 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4801 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4861 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4921 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4981 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5041 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5101 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5161 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5221 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5281 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5341 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5401 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5461 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5521 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5581 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5641 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5701 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5761 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5821 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5881 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5941 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6001 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6061 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6121 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6181 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6241 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6301 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6361 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6421 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6481 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6541 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6601 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6661 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6721 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6781 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6841 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6901 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6961 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7021 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7081 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7141 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7201 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7261 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7321 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7381 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7441 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7501 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7561 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7621 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7681 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7741 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7801 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7861 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7921 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7981 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8041 AAATTTAACG CGAATTTT
//