LOCUS       RC217988                7725 bp    DNA     circular  SYN 
DEFINITION  AKAP2 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 2 (AKAP2), transcript variant 1
ACCESSION   NM_001004065
VERSION     NM_001004065.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3882..3973 
                     /label="Myc-DDK"  
     misc_feature    4003..4588 
                     /label="PolyA signal"  
     misc_feature    4060..4079 
                     /label="Restriction Enzyme"  
     misc_feature    4864..5536 
                     /label="ColE1"  
     misc_feature    5966..6760 
                     /label="Kan/Neo"  
     misc_feature    6795..7097 
                     /label="SV40 ori"  
     misc_feature    7183..7660 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3864..3870 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3864 
                     /label="AKAP2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGCTGGCCC CAGCCCGGGG CTGCCGCTCG CCTTCCCCCG GAGTCTCCTG 
     1081 GACCCCCGGA GTCTCCTGGA CCCCCGGAGC GGGAGGCAGC GGCCGCGCGG CGCTGGACTG 
     1141 GAGCAGAACC CCAGGACTGC GCCCCCGGGA GCGGGCGCCC AGAGAAGCCT CCCCAGCTTT 
     1201 CTGAGGATGA TATCTGGCTA AAAAGCGAGG GAGACAACTA TAGTGCCACC CTCCTGGAGC 
     1261 CTGCTGCCAG CTCTCTTTCC CCAGATCACA AAAACATGGA AATTGAGGTG TCTGTTGCAG 
     1321 AATGTAAAAG TGTTCCTGGA ATCACCTCTA CCCCACATCC CATGGACCAT CCCTCCGCTT 
     1381 TCTATTCACC CCCGCATAAT GGCCTCCTTA CTGATCACCA CGAATCCCTG GATAATGATG 
     1441 TTGCCAGAGA GATCCGCTAT CTAGATGAGG TGCTAGAGGC CAACTGCTGT GATTCTGCTG 
     1501 TGGATGGAAC GTACAATGGA ACATCCTCCC CAGAGCCTGG TGCAGTGGTT CTGGTGGGCG 
     1561 GCCTAAGCCC CCCTGTCCAC GAGGCGACCC AGCCAGAACC CACTGAAAGA ACAGCTAGCC 
     1621 GGCAGGCACC TCCTCACATC GAGCTCAGTA ATAGCAGCCC TGACCCCATG GCAGAGGCAG 
     1681 AAAGAACAAA TGGCCATTCC CCCAGCCAGC CTAGAGATGC GCTGGGGGAC AGCCTGCAGG 
     1741 TGCCTGTCAG CCCCAGCTCC ACAACCAGCT CACGGTGTTC TTCCCGAGAT GGAGAGTTCA 
     1801 CTCTCACCAC ACTGAAAAAG GAGGCCAAGT TTGAGCTGCG TGCCTTCCAT GAGGACAAGA 
     1861 AGCCCTCCAA GCTCTTTGAG GATGACGAGC ATGAGAAAGA ACAATACTGC ATTAGAAAAG 
     1921 TGAGGCCTTC AGAGGAGATG CTGGAGCTGG AAAAGGAGAG GAGAGAGCTC ATCCGCAGCC 
     1981 AGGCCGTCAA GAAGAATCCT GGCATTGCAG CAAAATGGTG GAATCCCCCG CAGGAAAAAA 
     2041 CCATCGAGGA GCAGCTGGAC GAGGAACATC TGGAGTCGCA CAAAAAGTAC AAGGAGCGCA 
     2101 AAGAGAGAAG GGCACAGCAG GAACAGTTGC TGCTGCAGAA GCAGTTACAG CAGCAGCAGC 
     2161 AGCAGCCCCC ATCGCAGCTC TGCACAGCCC CTGCCTCTTC TCATGAACGC GCAAGCATGA 
     2221 TTGACAAAGC AAAGGAGGAC ATTGTCACAG AGCAGATAGA TTTCTCTGCT GCTCGCAAAC 
     2281 AATTTCAGCT GATGGAGAAT TCCAGGCAAG CGGTGGCCAA GGGCCAGAGT ACACCCAGGC 
     2341 TGTTCTCCAT CAAGCCTTTC TACAGGCCTC TGGGGTCAGT CAACTCAGAC AAGCCACTGA 
     2401 CTAATCCGAG ACCACCTTCT GTCGGGGGAC CTCCAGAAGA CAGTGGTGCC TCAGCCGCCA 
     2461 AGGGACAGAA ATCCCCCGGT GCCCTGGAGA CCCCATCGGC AGCAGGAAGC CAGGGCAACA 
     2521 CAGCCTCTCA GGGGAAGGAA GGGCCCTACA GCGAGCCTTC TAAACGTGGG CCCTTATCTA 
     2581 AACTGTGGGC TGAGGATGGA GAATTTACGA GCGCCCGGGC TGTCCTCACT GTGGTCAAGG 
     2641 ATGATGACCA TGGGATTTTG GATCAGTTCT CAAGATCTGT CAATGTCTCC TTGACCCAAG 
     2701 AGGAGCTTGA CTCTGGTCTG GACGAATTGT CGGTGAGGTC TCAGGATACC ACAGTCCTGG 
     2761 AGACCCTATC CAATGATTTC AGCATGGACA ACATCAGTGA CAGCGGGGCA TCCAATGAGA 
     2821 CAACCAATGC CCTCCAGGAA AATTCACTGG CTGATTTTTC TCTGCCCCAG ACACCACAAA 
     2881 CTGACAACCC CTCAGAGGGC CGAGGAGAAG GCGTCTCCAA GTCATTTAGT GATCATGGTT 
     2941 TCTATTCCCC TTCCTCCACG CTGGGGGACT CTCCGTTGGT TGATGACCCC TTGGAGTATC 
     3001 AGGCTGGCCT CCTGGTGCAG AATGCCATTC AACAAGCCAT AGCCGAGCAG GTGGATAAAG 
     3061 CTGTGTCCAA AACCAGCAGG GATGGAGCAG AGCAACAGGG ACCTGAAGCG ACTGTAGAGG 
     3121 AAGCTGAAGC TGCGGCTTTC GGCTCAGAAA AGCCTCAGAG CATGTTTGAG CCACCTCAGG 
     3181 TGTCTTCTCC TGTTCAAGAG AAAAGGGATG TATTACCAAA GATTCTGCCT GCTGAAGACA 
     3241 GGGCGCTCAG GGAAAGGGGG CCCCCCCAGC CACTGCCAGC TGTGCAGCCC AGTGGCCCGA 
     3301 TTAACATGGA GGAGACCAGG CCCGAAGGAA GCTATTTCAG CAAGTACTCG GAGGCAGCTG 
     3361 AGCTGAGAAG CACAGCCTCC CTCCTGGCCA CTCAAGAATC TGACGTGATG GTTGGGCCTT 
     3421 TCAAGCTGAG GTCCAGGAAA CAGCGGACTT TGTCCATGAT TGAGGAAGAG ATCCGAGCAG 
     3481 CTCAGGAAAG GGAAGAGGAG CTGAAGAGGC AGAGACAAGT CTTGCAGAGT ACGCAGAGCC 
     3541 CCAGGACAAA GAATGCCCCA TCACTGCCCT CCAGAACATG CTACAAAACT GCTCCAGGGA 
     3601 AAATAGAGAA AGTCAAACCT CCTCCATCCC CCACCACTGA AGGCCCCAGC TTGCAGCCTG 
     3661 ACTTAGCCCC TGAAGAGGCT GCCGGAACCC AGCGGCCCAA GAATCTGATG CAGACCCTCA 
     3721 TGGAAGACTA TGAGACACAC AAATCTAAAA GGCGCGAGAG AATGGATGAT AGTAGTGTCC 
     3781 TCGAGGCCAC ACGGGTTAAT CGAAGAAAGA GCGCACTGGC TTTGCGCTGG GAAGCAGGGA 
     3841 TCTATGCCAA CCAGGAGGAA GAAGACAACG AAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     3901 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     3961 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4021 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4081 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4141 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4201 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4261 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4321 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4381 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4441 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4501 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4561 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4621 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4681 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4741 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     4801 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     4861 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     4921 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     4981 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5041 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5101 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5161 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5221 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5281 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5341 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5401 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5461 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5521 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5581 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5641 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5701 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     5761 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     5821 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     5881 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     5941 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6001 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6061 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6121 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6181 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6241 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6301 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6361 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6421 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6481 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6541 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6601 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6661 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6721 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     6781 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     6841 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     6901 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     6961 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7021 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7081 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7141 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7201 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7261 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7321 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7381 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7441 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7501 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7561 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7621 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7681 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//