LOCUS       RC217976                9447 bp    DNA     circular  SYN 
DEFINITION  ARHGEF11 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 11 (ARHGEF11), transcript variant 1
ACCESSION   NM_014784
VERSION     NM_014784.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5604..5695 
                     /label="Myc-DDK"  
     misc_feature    5725..6310 
                     /label="PolyA signal"  
     misc_feature    5782..5801 
                     /label="Restriction Enzyme"  
     misc_feature    6586..7258 
                     /label="ColE1"  
     misc_feature    7688..8482 
                     /label="Kan/Neo"  
     misc_feature    8517..8819 
                     /label="SV40 ori"  
     misc_feature    8905..9382 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5586..5592 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5586 
                     /label="ARHGEF11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGTAAGG TTACCCCAGA GTATAGACAG GTTAAGTAGC CTGTCTTCTC 
     1081 TGGGAGATTC TGCACCAGAG CGCAAGTCCC CTTCCCACCA TCGCCAGCCT TCGGATGCCT 
     1141 CTGAGACAAC AGGTCTCGTT CAACGCTGTG TCATTATCCA AAAGGACCAG CATGGCTTCG 
     1201 GCTTCACAGT CAGTGGGGAT CGCATTGTTC TGGTGCAGTC TGTGCGGCCT GGAGGTGCAG 
     1261 CCATGAAGGC CGGTGTGAAA GAGGGCGACC GGATCATCAA AGTCAACGGC ACCATGGTGA 
     1321 CCAATAGCTC ACACCTGGAA GTGGTAAAGC TGATCAAATC TGGCGCCTAT GTCGCACTCA 
     1381 CCCTCCTGGG CTCTTCACCT TCATCCATGG GCATCTCTGG GCTCCAGCAG GACCCATCCC 
     1441 CAGCAGGAGC TCCCCGAATC ACGTCAGTGA TCCCCTCACC ACCACCTCCT CCACCTCTAC 
     1501 CACCTCCACA ACGCATCACA GGACCCAAAC CTCTGCAGGA TCCCGAAGTT CAAAAACATG 
     1561 CCACCCAGAT CCTCAGGAAT ATGCTGAGGC AGGAAGAAAA AGAATTACAG GACATACTTC 
     1621 CACTATATGG TGACACCAGC CAGAGACCAT CAGAAGGCCG GCTCTCTCTG GATTCCCAGG 
     1681 AGGGGGACAG TGGCTTGGAC TCTGGGACAG AACGCTTTCC TTCCCTCAGT GAGTCATTGA 
     1741 TGAATCGGAA CTCGGTACTG TCAGACCCTG GGCTAGACAG TCCTCGAACC TCCCCTGTGA 
     1801 TCATGGCCAG GGTGGCCCAG CACCACAGGC GGCAGGGCTC GGATGCAGCA GTCCCCTCAA 
     1861 CCGGTGACCA GGGTGTAGAT CAAAGCCCAA AGCCTTTAAT TATTGGCCCA GAGGAAGACT 
     1921 ATGACCCGGG TTATTTCAAC AACGAGAGCG ACATCATATT CCAGGATCTG GAGAAACTGA 
     1981 AGTCTCGGCC AGCTCACCTG GGGGTTTTTC TACGTTACAT CTTCTCTCAG GCGGACCCCA 
     2041 GTCCACTGCT TTTTTACCTG TGTGCAGAAG TTTATCAGCA GGCAAGCCCC AAGGATTCCC 
     2101 GAAGCTTGGG GAAAGACATC TGGAATATTT TCCTGGAGAA AAATGCGCCT CTGAGAGTGA 
     2161 AGATCCCTGA GATGCTACAG GCTGAAATTG ACTCGCGCCT GCGGAACAGC GAAGATGCCC 
     2221 GTGGTGTTCT CTGTGAAGCT CAAGAGGCAG CCATGCCTGA GATCCAAGAG CAGATCCACG 
     2281 ACTACAGAAC GAAGCGCACA CTGGGGCTGG GCAGCCTGTA TGGTGAAAAT GACCTGCTGG 
     2341 ACCTGGATGG GGACCCTCTC CGAGAGCGCC AAGTGGCTGA GAAGCAGCTG GCTGCCCTTG 
     2401 GAGATATTTT GTCCAAGTAT GAGGAAGACA GGAGCGCCCC CATGGACTTC GCCCTCAATA 
     2461 CCTACATGAG CCATGCTGGG ATCCGTCTTC GAGAGGCACG ACCTTCCAAC ACAGCTGAAA 
     2521 AGGCCCAGTC TGCTCCTGAC AAGGACAAGT GGCTACCGTT CTTCCCTAAG ACCAAGAAGA 
     2581 GCAGCAATTC CAAGAAAGAA AAGGATGCCT TGGAGGACAA GAAGCGAAAC CCTATCCTCA 
     2641 AATACATTGG GAAGCCCAAA AGCTCTTCTC AAAGCACATT TCATATTCCC TTGTCCCCTG 
     2701 TGGAAGTCAA ACCAGGCAAT GTGAGGAACA TCATTCAGCA CTTTGAGAAC AACCAGCAGT 
     2761 ATGATGCCCC AGAACCTGGG ACACAACGAC TCTCGACCGG AAGCTTTCCT GAGGACCTGC 
     2821 TGGAGAGTGA CAGTTCACGC TCAGAGATTC GCCTGGGCCG CTCTGAAAGC CTCAAGGGCC 
     2881 GGGAAGAGAT GAAACGGTCT CGAAAGGCAG AGAACGTGCC CCGCTCTCGC AGTGATGTTG 
     2941 ACATGGATGC TGCTGCGGAG GCTACTCGCC TGCACCAGTC AGCCTCGTCC TCTACCTCCA 
     3001 GCCTCTCCAC CAGGTCTCTT GAGAACCCAA CCCCTCCATT CACTCCCAAA ATGGGCCGCA 
     3061 GGAGCATTGA GTCCCCCAGT TTGGGGTTCT GCACAGATAC CCTCCTTCCC CACCTCCTAG 
     3121 AGGATGATCT GGGCCAGCTG TCTGACCTGG AGCCAGAGCC AGATGCCCAA AATTGGCAGC 
     3181 ATACAGTGGG CAAGGATGTG GTGGCTGGGC TAACCCAGCG GGAGATTGAC CGGCAAGAGG 
     3241 TCATCAATGA GCTGTTTGTG ACTGAAGCTT CCCACCTGCG CACACTCCGG GTCCTGGACC 
     3301 TGATCTTCTA CCAGCGAATG AAGAAGGAGA ACCTGATGCC CCGGGAGGAG CTGGCCCGGC 
     3361 TCTTCCCGAA CCTGCCTGAA CTCATAGAGA TTCACAATTC CTGGTGTGAA GCCATGAAGA 
     3421 AGCTCCGGGA GGAAGGCCCC ATCATCAAAG AGATCAGTGA CCTCATGCTG GCCCGGTTTG 
     3481 ATGGCCCTGC CCGAGAGGAA CTCCAGCAAG TGGCTGCACA GTTCTGTTCC TATCAGTCAA 
     3541 TAGCCCTAGA GCTAATCAAG ACCAAGCAAC GCAAGGAGAG TCGATTCCAG CTCTTCATGC 
     3601 AGGAGGCTGA GAGCCACCCT CAGTGTCGGC GGCTGCAGCT GAGAGACCTC ATCATCTCTG 
     3661 AGATGCAGCG GCTCACCAAG TACCCGCTGC TGCTGGAGAG CATCATCAAG CACACAGAGG 
     3721 GTGGCACCTC TGAGCATGAG AAGCTGTGCC GGGCCCGGGA CCAGTGCCGG GAGATTCTCA 
     3781 AGTATGTGAA TGAAGCGGTA AAACAAACAG AGAACCGCCA CCGTTTAGAG GGCTACCAGA 
     3841 AACGCCTGGA TGCCACCGCC CTGGAGAGGG CCAGCAACCC CCTGGCAGCA GAGTTCAAGA 
     3901 GCCTGGATCT TACAACCAGA AAAATGATCC ATGAGGGACC CCTGACCTGG AGGATCAGCA 
     3961 AGGATAAGAC CTTGGACCTC CACGTGCTGC TGCTGGAGGA CCTCCTAGTG CTGCTACAGA 
     4021 AACAGGATGA GAAGCTATTG CTGAAGTGCC ACAGCAAGAC TGCTGTGGGC TCCTCAGACA 
     4081 GCAAGCAGAC CTTCAGCCCC GTGCTCAAGC TCAATGCTGT GCTCATCCGC TCTGTGGCCA 
     4141 CAGATAAACG GGCCTTCTTC ATCATCTGCA CCTCCAAGCT GGGCCCACCC CAGATCTATG 
     4201 AGCTGGTTGC ATTGACGTCA TCAGACAAGA ACACATGGAT GGAGCTCTTA GAAGAGGCCG 
     4261 TGCGGAATGC CACCAGGCAC CCCGGAGCTG CCCCAATGCC CGTCCATCCT CCACCCCCAG 
     4321 GTCCCCGGGA GCCAGCCCAG CAGGGCCCCA CACCCAGCAG GGTAGAACTG GATGACTCAG 
     4381 ACGTGTTCCA TGGTGAACCT GAACCTGAGG AGCTGCCTGG AGGCACTGGG TCCCAGCAGA 
     4441 GGGTCCAAGG GAAGCACCAG GTCCTGCTAG AGGACCCTGA GCAGGAGGGC AGTGCAGAGG 
     4501 AAGAGGAACT GGGTGTCCTG CCTTGCCCTT CCACATCCCT GGATGGAGAG AACAGGGGCA 
     4561 TCAGGACAAG GAACCCCATC CACTTGGCCT TCCCAGGCCC TCTGTTCATG GAAGGGCTCG 
     4621 CTGACTCCGC TCTGGAAGAT GTGGAGAACC TGCGACATCT GATCCTGTGG AGCCTGCTGC 
     4681 CAGGTCACAC CATGGAAACT CAGGCTGCCC AGGAGCCCGA GGACGACCTG ACACCCACAC 
     4741 CTTCTGTCAT CAGCGTCACC TCTCACCCCT GGGACCCAGG CTCCCCAGGG CAAGCACCCC 
     4801 CTGGGGGTGA AGGGGACAAC ACCCAGCTTG CAGGGCTGGA GGGGGAACGG CCAGAGCAGG 
     4861 AAGACATGGG TCTCTGTTCT CTGGAACACC TACCCCCAAG GACCAGAAAT TCTGGGATAT 
     4921 GGGAGTCTCC AGAACTGGAC AGGAATCTGG CTGAAGATGC TTCAAGCACA GAGGCAGCAG 
     4981 GAGGTTACAA AGTTGTGAGA AAAGCTGAGG TGGCAGGCAG CAAGGTTGTC CCTGCACTAC 
     5041 CAGAGAGTGG CCAGTCAGAG CCTGGGCCAC CTGAAGTGGA AGGCGGAACA AAGGCTACGG 
     5101 GGAACTGCTT TTATGTCAGC ATGCCATCAG GACCCCCGGA CTCAAGCACC GACCACTCAG 
     5161 AGGCACCCAT GAGCCCCCCT CAGCCTGACA GCCTCCCTGC AGGGCAGACA GAGCCTCAGC 
     5221 CTCAGCTGCA GGGAGGCAAC GATGATCCAA GACGCCCCAG CCGCTCTCCT CCAAGCCTGG 
     5281 CCCTCAGGGA CGTGGGCATG ATCTTCCATA CCATTGAGCA GCTCACTCTC AAGCTCAACA 
     5341 GGCTCAAGGA TATGGAGCTG GCCCACAGAG AGCTGCTCAA GTCCCTTGGG GGAGAGTCAT 
     5401 CTGGTGGCAC CACGCCTGTG GGCAGTTTCC ACACAGAAGC AGCTAGATGG ACAGATGGCT 
     5461 CCCTCTCACC TCCCGCTAAG GAGCCCCTAG CTTCTGACTC CAGGAACAGC CATGAACTGG 
     5521 GGCCCTGCCC TGAGGATGGC TCTGACGCCC CCCTGGAAGA CAGCACAGCA GACGCAGCCG 
     5581 CGTCACCAGG ACCAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5641 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5701 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5761 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5821 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5881 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5941 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     6001 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     6061 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     6121 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6181 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6241 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6301 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6361 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6421 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6481 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6541 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6601 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6661 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6721 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6781 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6841 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6901 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6961 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     7021 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     7081 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     7141 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7201 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7261 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7321 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7381 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7441 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7501 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7561 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7621 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7681 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7741 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7801 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7861 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7921 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7981 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     8041 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     8101 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     8161 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8221 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8281 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8341 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8401 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8461 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8521 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8581 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8641 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8701 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8761 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8821 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8881 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8941 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     9001 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     9061 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     9121 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9181 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9241 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9301 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9361 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9421 ATTTAACAAA AATTTAACGC GAATTTT
//