LOCUS       RC217928                8253 bp    DNA     circular  SYN 
DEFINITION  JAK3 (Myc-DDK-tagged)-Human Janus kinase 3 (JAK3)
ACCESSION   NM_000215
VERSION     NM_000215.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4410..4501 
                     /label="Myc-DDK"  
     misc_feature    4531..5116 
                     /label="PolyA signal"  
     misc_feature    4588..4607 
                     /label="Restriction Enzyme"  
     misc_feature    5392..6064 
                     /label="ColE1"  
     misc_feature    6494..7288 
                     /label="Kan/Neo"  
     misc_feature    7323..7625 
                     /label="SV40 ori"  
     misc_feature    7711..8188 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4392..4398 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4392 
                     /label="JAK3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACCTCCA AGTGAAGAGA CGCCCCTGAT CCCTCAGCGT TCATGCAGCC 
     1081 TCTTGTCCAC GGAGGCTGGT GCCCTGCATG TGCTGCTGCC CGCTCGGGGC CCCGGGCCCC 
     1141 CCCAGCGCCT ATCTTTCTCC TTTGGGGACC ACTTGGCTGA GGACCTGTGC GTGCAGGCTG 
     1201 CCAAGGCCAG CGGCATCCTG CCTGTGTACC ACTCCCTCTT TGCTCTGGCC ACGGAGGACC 
     1261 TGTCCTGCTG GTTCCCCCCG AGCCACATCT TCTCCGTGGA GGATGCCAGC ACCCAAGTCC 
     1321 TGCTGTACAG GATTCGCTTT TACTTCCCCA ATTGGTTTGG GCTGGAGAAG TGCCACCGCT 
     1381 TCGGGCTACG CAAGGATTTG GCCAGTGCTA TCCTTGACCT GCCAGTCCTG GAGCACCTCT 
     1441 TTGCCCAGCA CCGCAGTGAC CTGGTGAGTG GGCGCCTCCC CGTGGGCCTC AGTCTCAAGG 
     1501 AGCAGGGTGA GTGTCTCAGC CTGGCCGTGT TGGACCTGGC CCGGATGGCG CGAGAGCAGG 
     1561 CCCAGCGGCC GGGAGAGCTG CTGAAGACTG TCAGCTACAA GGCCTGCCTA CCCCCAAGCC 
     1621 TGCGCGACCT GATCCAGGGC CTGAGCTTCG TGACGCGGAG GCGTATTCGG AGGACGGTGC 
     1681 GCAGAGCCCT GCGCCGCGTG GCCGCCTGCC AGGCAGACCG GCACTCGCTC ATGGCCAAGT 
     1741 ACATCATGGA CCTGGAGCGG CTGGATCCAG CCGGGGCCGC CGAGACCTTC CACGTGGGCC 
     1801 TCCCTGGGGC CCTTGGTGGC CACGACGGGC TGGGGCTGCT CCGCGTGGCT GGTGACGGCG 
     1861 GCATCGCCTG GACCCAGGGA GAACAGGAGG TCCTCCAGCC CTTCTGCGAC TTTCCAGAAA 
     1921 TCGTAGACAT TAGCATCAAG CAGGCCCCGC GCGTTGGCCC GGCCGGAGAG CACCGCCTGG 
     1981 TCACTGTTAC CAGGACAGAC AACCAGATTT TAGAGGCCGA GTTCCCAGGG CTGCCCGAGG 
     2041 CTCTGTCGTT CGTGGCGCTC GTGGACGGCT ACTTCCGGCT GACCACGGAC TCCCAGCACT 
     2101 TCTTCTGCAA GGAGGTGGCA CCGCCGAGGC TGCTGGAGGA AGTGGCCGAG CAGTGCCACG 
     2161 GCCCCATCAC TCTGGACTTT GCCATCAACA AGCTCAAGAC TGGGGGCTCA CGTCCTGGCT 
     2221 CCTATGTTCT CCGCCGCAGC CCCCAGGACT TTGACAGCTT CCTCCTCACT GTCTGTGTCC 
     2281 AGAACCCCCT TGGTCCTGAT TATAAGGGCT GCCTCATCCG GCGCAGCCCC ACAGGAACCT 
     2341 TCCTTCTGGT TGGCCTCAGC CGACCCCACA GCAGTCTTCG AGAGCTCCTG GCAACCTGCT 
     2401 GGGATGGGGG GCTGCACGTA GATGGGGTGG CAGTGACCCT CACTTCCTGC TGTATCCCCA 
     2461 GACCCAAAGA AAAGTCCAAC CTGATCGTGG TCCAGAGAGG TCACAGCCCA CCCACATCAT 
     2521 CCTTGGTTCA GCCCCAATCC CAATACCAGC TGAGTCAGAT GACATTTCAC AAGATCCCTG 
     2581 CTGACAGCCT GGAGTGGCAT GAGAACCTGG GCCATGGGTC CTTCACCAAG ATTTACCGGG 
     2641 GCTGTCGCCA TGAGGTGGTG GATGGGGAGG CCCGAAAGAC AGAGGTGCTG CTGAAGGTCA 
     2701 TGGATGCCAA GCACAAGAAC TGCATGGAGT CATTCCTGGA AGCAGCGAGC TTGATGAGCC 
     2761 AAGTGTCGTA CCGGCATCTC GTGCTGCTCC ACGGCGTGTG CATGGCTGGA GACAGCACCA 
     2821 TGGTGCAGGA ATTTGTACAC CTGGGGGCCA TAGACATGTA TCTGCGAAAA CGTGGCCACC 
     2881 TGGTGCCAGC CAGCTGGAAG CTGCAGGTGG TCAAACAGCT GGCCTACGCC CTCAACTATC 
     2941 TGGAGGACAA AGGCCTGCCC CATGGCAATG TCTCTGCCCG GAAGGTGCTC CTGGCTCGGG 
     3001 AGGGGGCTGA TGGGAGCCCG CCCTTCATCA AGCTGAGTGA CCCTGGGGTC AGCCCCGCTG 
     3061 TGTTAAGCCT GGAGATGCTC ACCGACAGGA TCCCCTGGGT GGCCCCCGAG TGTCTCCGGG 
     3121 AGGCGCAGAC ACTTAGCTTG GAAGCTGACA AGTGGGGCTT CGGCGCCACG GTCTGGGAAG 
     3181 TGTTTAGTGG CGTCACCATG CCCATCAGTG CCCTGGATCC TGCTAAGAAA CTCCAATTTT 
     3241 ATGAGGACCG GCAGCAGCTG CCGGCCCCCA AGTGGACAGA GCTGGCCCTG CTGATTCAAC 
     3301 AGTGCATGGC CTATGAGCCG GTCCAGAGGC CCTCCTTCCG AGCCGTCATT CGTGACCTCA 
     3361 ATAGCCTCAT CTCTTCAGAC TATGAGCTCC TCTCAGACCC CACACCTGGT GCCCTGGCAC 
     3421 CTCGTGATGG GCTGTGGAAT GGTGCCCAGC TCTATGCCTG CCAAGACCCC ACGATCTTCG 
     3481 AGGAGAGACA CCTCAAGTAC ATCTCACAGC TGGGCAAGGG CAACTTTGGC AGCGTGGAGC 
     3541 TGTGCCGCTA TGACCCGCTA GGCGACAATA CAGGTGCCCT GGTGGCCGTG AAACAGCTGC 
     3601 AGCACAGCGG GCCAGACCAG CAGAGGGACT TTCAGCGGGA GATTCAGATC CTCAAAGCAC 
     3661 TGCACAGTGA TTTCATTGTC AAGTATCGTG GTGTCAGCTA TGGCCCGGGC CGCCAGAGCC 
     3721 TGCGGCTGGT CATGGAGTAC CTGCCCAGCG GCTGCTTGCG CGACTTCCTG CAGCGGCACC 
     3781 GCGCGCGCCT CGATGCCAGC CGCCTCCTTC TCTATTCCTC GCAGATCTGC AAGGGCATGG 
     3841 AGTACCTGGG CTCCCGCCGC TGCGTGCACC GCGACCTGGC CGCCCGAAAC ATCCTCGTGG 
     3901 AGAGCGAGGC ACACGTCAAG ATCGCTGACT TCGGCCTAGC TAAGCTGCTG CCGCTTGACA 
     3961 AAGACTACTA CGTGGTCCGC GAGCCAGGCC AGAGCCCCAT TTTCTGGTAT GCCCCCGAAT 
     4021 CCCTCTCGGA CAACATCTTC TCTCGCCAGT CAGACGTCTG GAGCTTCGGG GTCGTCCTGT 
     4081 ACGAGCTCTT CACCTACTGC GACAAAAGCT GCAGCCCCTC GGCCGAGTTC CTGCGGATGA 
     4141 TGGGATGTGA GCGGGATGTC CCCGCCCTCT GCCGCCTCTT GGAACTGCTG GAGGAGGGCC 
     4201 AGAGGCTGCC GGCGCCTCCT GCCTGCCCTG CTGAGGTTCA CGAGCTCATG AAGCTGTGCT 
     4261 GGGCCCCTAG CCCACAGGAC CGGCCATCAT TCAGCGCCCT GGGCCCCCAG CTGGACATGC 
     4321 TGTGGAGCGG AAGCCGGGGG TGTGAGACTC ATGCCTTCAC TGCTCACCCA GAGGGCAAAC 
     4381 ACCACTCCCT GTCCTTTTCA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4441 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4501 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4561 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4621 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4681 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4741 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4801 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4861 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4921 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4981 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5041 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5101 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5161 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5221 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5281 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5341 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5401 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5461 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5521 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5581 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5641 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5701 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5761 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5821 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5881 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5941 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6001 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6061 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6121 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6181 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6241 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6301 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6361 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6421 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6481 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6541 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6601 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6661 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6721 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6781 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6841 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6901 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6961 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7021 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7081 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7141 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7201 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7261 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7321 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7381 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7441 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7501 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7621 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7681 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7741 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7801 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7861 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7921 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7981 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8041 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8101 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8161 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8221 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//