LOCUS       RC217903                8406 bp    DNA     circular  SYN 
DEFINITION  PLCB4 (Myc-DDK-tagged)-Human phospholipase C, beta 4 (PLCB4), transcript variant 2
ACCESSION   NM_182797
VERSION     NM_182797.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4563..4654 
                     /label="Myc-DDK"  
     misc_feature    4684..5269 
                     /label="PolyA signal"  
     misc_feature    4741..4760 
                     /label="Restriction Enzyme"  
     misc_feature    5545..6217 
                     /label="ColE1"  
     misc_feature    6647..7441 
                     /label="Kan/Neo"  
     misc_feature    7476..7778 
                     /label="SV40 ori"  
     misc_feature    7864..8341 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4545..4551 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4545 
                     /label="PLCB4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCAAACCT TATGAATTTA ACTGGCAGAA GGAAGTTCCC TCCTTTTTGC 
     1081 AAGAAGGAGC AGTTTTTGAC AGATACGAGG AGGAATCCTT TGTGTTTGAA CCCAACTGCC 
     1141 TCTTCAAAGT GGATGAGTTT GGCTTCTTTC TGACATGGAG AAGTGAAGGC AAGGAAGGAC 
     1201 AGGTGCTAGA ATGCTCCCTC ATCAACAGTA TTCGGTCGGG AGCCATACCA AAGGATCCCA 
     1261 AAATCTTGGC TGCTCTTGAA GCTGTTGGAA AATCAGAAAA TGATCTGGAA GGGCGGATAG 
     1321 TTTGTGTCTG CAGTGGCACA GATCTAGTGA ACATTAGTTT TACCTACATG GTGGCTGAAA 
     1381 ATCCAGAAGT AACTAAGCAA TGGGTAGAAG GCCTGAGATC AATCATACAC AACTTCAGGG 
     1441 CCAACAACGT CAGTCCAATG ACATGCCTCA AGAAACACTG GATGAAATTG GCATTTATGA 
     1501 CCAACACAAA TGGTAAAATT CCAGTTAGGA GTATTACTAG AACATTTGCA TCGGGAAAAA 
     1561 CAGAAAAGGT GATCTTTCAA GCACTCAAGG AGTTAGGTCT TCCCAGTGGA AAGAATGATG 
     1621 AAATTGAGCC CACAGCATTT TCTTATGAAA AGTTCTATGA ACTGACACAA AAGATTTGTC 
     1681 CTCGGACAGA TATAGAAGAT CTTTTCAAAA AAATCAATGG AGACAAAACT GATTATTTAA 
     1741 CGGTAGACCA ATTAGTGAGC TTTCTAAATG AACATCAACG AGATCCTCGA TTGAATGAAA 
     1801 TTTTATTTCC ATTTTATGAT GCCAAAAGGG CAATGCAGAT CATTGAGATG TATGAACCTG 
     1861 ATGAAGATTT GAAGAAAAAA GGCCTTATAT CAAGTGATGG GTTTTGCAGA TATCTGATGT 
     1921 CAGATGAAAA CGCCCCAGTC TTCCTAGATC GTTTAGAACT TTACCAAGAA ATGGACCATC 
     1981 CTCTGGCTCA CTACTTCATC AGTTCTTCCC ATAACACTTA TCTCACTGGC AGACAGTTCG 
     2041 GCGGGAAGTC TTCGGTAGAA ATGTACAGAC AGGTTCTCCT GGCTGGTTGC AGATGTGTTG 
     2101 AACTTGACTG CTGGGATGGA AAAGGTGAAG ACCAAGAACC AATAATAACT CATGGAAAAG 
     2161 CAATGTGTAC AGATATCCTT TTTAAGGATG TAATTCAAGC CATCAAGGAA ACTGCATTTG 
     2221 TCACATCAGA ATATCCTGTA ATTCTCTCCT TTGAAAATCA CTGCAGCAAA TATCAACAGT 
     2281 ACAAGATGTC CAAATATTGC GAAGATCTAT TTGGGGATCT CCTGTTGAAA CAAGCACTTG 
     2341 AATCACATCC ACTTGAACCA GGCAGGGCTT TGCCATCCCC CAATGACCTC AAAAGAAAAA 
     2401 TACTCATAAA AAACAAGCGG CTGAAACCTG AAGTTGAAAA AAAACAGCTG GAAGCTTTGA 
     2461 GAAGCATGAT GGAAGCTGGA GAATCTGCCT CCCCAGCAAA CATCTTAGAG GACGATAATG 
     2521 AAGAGGAGAT CGAAAGTGCT GACCAAGAGG AGGAAGCTCA CCCCGAATTC AAATTTGGAA 
     2581 ATGAACTTTC TGCTGATGAC TTGGGTCACA AGGAAGCTGT TGCAAATAGC GTCAAGAAGG 
     2641 GCCTGGTCAC TGTAGAAGAT GAGCAGGCGT GGATGGCATC TTATAAATAT GTAGGTGCTA 
     2701 CCACTAATAT CCATCCATAT TTGTCCACAA TGATCAACTA CGCCCAGCCT GTAAAGTTTC 
     2761 AAGGTTTCCA TGTGGCAGAA GAACGCAATA TTCATTATAA CATGTCTTCT TTTAATGAAT 
     2821 CAGTCGGTCT TGGCTACTTG AAGACACATG CAATTGAATT TGTCAATTAT AACAAACGGC 
     2881 AAATGAGTCG CATTTACCCC AAGGGAGGCC GAGTCGATTC CAGTAATTAC ATGCCTCAGA 
     2941 TTTTCTGGAA CGCTGGCTGC CAGATGGTTT CACTGAACTA TCAAACCCCA GATTTAGCGA 
     3001 TGCAATTGAA TCAGGGAAAA TTTGAGTATA ATGGATCGTG CGGGTACCTT CTCAAACCAG 
     3061 ATTTCATGAG GCGGCCTGAT CGAACATTTG ACCCCTTCTC TGAAACTCCT GTTGATGGTG 
     3121 TTATTGCAGC CACTTGCTCA GTGCAGGTTA TATCAGGTCA ATTCTTATCA GATAAGAAAA 
     3181 TTGGCACCTA CGTAGAGGTG GATATGTATG GGTTGCCCAC TGACACCATA CGTAAGGAAT 
     3241 TCCGAACTCG CATGGTTATG AATAATGGAC TCAATCCAGT TTACAATGAA GAGTCATTTG 
     3301 TATTTCGGAA GGTGATCCTG CCGGACCTGG CTGTCTTGAG AATAGCTGTG TATGATGATA 
     3361 ACAACAAGCT GATTGGCCAG AGGATCCTCC CGCTTGATGG CCTCCAAGCC GGATATCGAC 
     3421 ACATTTCCCT TCGAAATGAG GGAAATAAAC CATTATCACT ACCAACAATT TTCTGCAATA 
     3481 TTGTTCTTAA AACATATGTG CCTGATGGAT TTGGAGATAT CGTGGATGCT TTATCAGATC 
     3541 CAAAGAAATT TCTCTCAATT ACAGAAAAGA GAGCAGACCA AATGAGAGCT ATGGGCATTG 
     3601 AAACTAGTGA CATAGCCGAC GTGCCCAGTG ACACTTCCAA AAATGACAAG AAAGGAAAGG 
     3661 CCAACACCGC CAAAGCAAAT GTGACCCCTC AGAGTAGCTC TGAGCTCAGA CCAACCACCA 
     3721 CGGCTGCCCT GGCCTCTGGT GTGGAAGCCA AGAAAGGTAT TGAACTTATC CCTCAAGTAA 
     3781 GGATAGAAGA CTTAAAGCAG ATGAAGGCTT ACTTGAAGCA TTTAAAGAAA CAGCAGAAGG 
     3841 AGCTAAATTC TTTAAAGAAG AAACATGCAA AGGAACACAG TACCATGCAG AAGTTACACT 
     3901 GCACGCAAGT TGACAAAATT GTGGCACAGT ATGACAAAGA GAAGTCGACT CATGAGAAAA 
     3961 TCCTAGAGAA GGCAATGAAG AAGAAGGGGG GAAGTAATTG TCTCGAAATG AAAAAAGAAA 
     4021 CAGAAATCAA AATTCAGACG CTGACATCAG ATCACAAATC TAAGGTCAAA GAGATTGTAG 
     4081 CACAGCACAC AAAGGAATGG TCAGAAATGA TCAATACCCA CAGTGCTGAG GAGCAAGAAA 
     4141 TCCGAGACCT GCACCTCAGC CAGCAGTGTG AGCTGCTGAA AAAGCTACTC ATCAATGCCC 
     4201 ACGAGCAGCA AACCCAGCAG CTGAAACTGT CCCATGACAG GGAAAGCAAG GAAATGCGAG 
     4261 CACACCAGGC TAAGATTTCT ATGGAAAATA GCAAAGCCAT CAGCCAAGAT AAATCTATCA 
     4321 AGAATAAAGC AGAACGGGAA AGGCGAGTCA GGGAGTTAAA CAGCAGCAAC ACTAAAAAGT 
     4381 TTCTGGAAGA AAGAAAGAGA CTTGCCATGA AGCAGTCCAA AGAAATGGAT CAGTTGAAAA 
     4441 AAGTCCAGCT TGAACATCTA GAATTCCTAG AGAAACAGAA TGAGCAGGCG AAGGAGATGC 
     4501 AGCAGATGGT GAAATTGGAA GCCGAGATGG ACCGCAGACC AGCAACAGTA GTAACGCGTA 
     4561 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4621 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4681 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4741 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4801 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4861 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4921 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4981 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5041 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5101 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5161 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5221 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5281 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5341 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5401 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5461 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5521 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5581 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5641 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5701 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5761 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5821 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5881 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5941 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6001 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6061 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6121 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6181 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6241 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6301 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6361 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6421 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6481 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6541 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6601 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6661 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6721 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6781 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6841 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6901 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6961 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7021 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7081 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7141 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7201 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7261 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7321 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7381 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7441 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7501 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7561 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7621 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7681 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7741 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7801 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7861 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7921 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7981 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8041 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8101 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8161 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8221 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8281 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8341 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8401 AATTTT
//