LOCUS       RC217878                9090 bp    DNA     circular  SYN 
DEFINITION  KIAA1009 (Myc-DDK-tagged)-Human KIAA1009 (KIAA1009)
ACCESSION   NM_014895
VERSION     NM_014895.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5247..5338 
                     /label="Myc-DDK"  
     misc_feature    5368..5953 
                     /label="PolyA signal"  
     misc_feature    5425..5444 
                     /label="Restriction Enzyme"  
     misc_feature    6229..6901 
                     /label="ColE1"  
     misc_feature    7331..8125 
                     /label="Kan/Neo"  
     misc_feature    8160..8462 
                     /label="SV40 ori"  
     misc_feature    8548..9025 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5229..5235 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5229 
                     /label="CEP162"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTAACTGT TCCCAAGAAG AGCTAGATGA AGAGTTTGAA CAGTTTATGA 
     1081 AAGAGCTTTC AGATGATTCT TTTGAAAATT CAGACAAAAC AGCTAGACAA TCTAAAAAAG 
     1141 AGATGAAGAA GAAAGATACA GTGCCTTGGT GGATAACTGA AGATGATTTT AAAGATGATG 
     1201 GACTTCTTGG AACAAATGTG AGCTATTTGA AAACAAAGAA GACTTCTCAG CCTGTTATGG 
     1261 AAATAGAAGA GGAGTCTGCT GAAAAGATTC AATTTCTTAA GAGCAGTGGA ACCTCTCTCT 
     1321 TAAGTACTGA TAGCTTAGAA ACAAATGAAC TAGTAGTTTC TGAGCTCAAC CATAGTAGTC 
     1381 TCGGAGTGGG ATTGGACACA TTAGAAGAAC AAGAGGAGAA AGAACAATTT TTTGCCAGGC 
     1441 TTGAGAAAGG CTTGACATCT TCCATTGATT ATTCGAGATT AAATAAGGAA TTGGATTCTA 
     1501 ATGACTCTAC ACATTTTAAA GCTTTACATA GTAATCAAGC CAACGCAGAA CTAACTGATG 
     1561 ACGAACATGA GAATGAATCG AAACATGAAG AACTGGCAGA AAATTACAGT GATGATTTTG 
     1621 AAGATGAGTA TGTTGGTGCA CCGTTGACTA CTAAAGATGA AGAGATGCCT TCCAAAGAGA 
     1681 ATTCCAAATC AGAAAAAATA AGTGTGCCCA AACAGGAAGA AGAAAAAACT GGCATGCTTG 
     1741 CTAATGTTGT GCTGCTTGAT TCATTAGACT CTGTTGCAGA GGTCAATCTT GATGAACAAG 
     1801 ATAAAATAAC ACCTAAGCCA AGGTGCCTAC CAGAAATGAC TGAGAATGAA ATGACAGGAA 
     1861 CAGGTGTTTC TTATGGACAA AGCAGTAGTG ACGTTGAAGC CCTACATCAA GCTTATTGTC 
     1921 ATATAGCCCA TTCATTGGGA GATGAAGACA AACAAAAAAT TGAGAGTAAC ACAGTGGAAG 
     1981 ATATCAAGAG CTCAGTGAAA GGTCATCCTC AAGAAAATGA AGAGAATTCA AAAAACATCT 
     2041 CTACTATGGA ATCTGATCTG CCCACAGTAG AGGAGCTGAT GAAACCTATC AGAATAGATT 
     2101 CCTTTGGGAT CAGTGGTTTT GATTTACAAC CTGTCAGCTC TGAGAAAGTG GCCGAAAGAA 
     2161 AAGAAACTGA ATTTTTTAGC TCTTTACCCC TGAAGATGAA CCCAAATATT TTGTCTCAAG 
     2221 ACTCACAACA TGTGAACCTT TTTTTTGACA AAAATGATGA GAATGTGATT TTACAAAAGA 
     2281 CCACAAATGA GAGTATGGAA AACAGCTGTC CACAAGTAAC TGAAGTAACT GCCACAGAAG 
     2341 AACATGTTGA TAAAATGTAC CTTAATATTT TGAGGAAAAA AATAACTGTT AATTCTTCAT 
     2401 CATTATCTCA GGATGACAAA ATTAATAAAA CTTACAGATC TCAACTTAGT TCTGAAGAAG 
     2461 AAGGGGCTGT AATGGGTAAA CAGGTACCAT ACAAGAAGGC CAGAAGTGCA CCTCCTTTAC 
     2521 TTAAAAGGAA ACCCCAGAGT GGATTATATG CGTCAGTTAG GAGCTCAGGC TATGGCAAAC 
     2581 CCAGTTCACC ACTCAAGATG TTTTCTACTC TTGAAAAGAA AACTTCAGAG GACATTATAA 
     2641 AAAGCAAAAA CTTGAGATCG ATTTCTACCT CCAATCAACC TAGGAAAAAA GAAATCTTAT 
     2701 CTGGAACAAA ACTCATCAAG CCTGCAGCTT TGGATAAACC AGCTCACAAA ACTGAAAGTT 
     2761 GCCTGTCTAC TCGTAAGAAG TCTGAAAATC CCACAGAAAC TGATTCCTGT ATTCAGTTTC 
     2821 AGACTGATTC CTTAGGATAC TGTGGTGAGA ACAAGGAGAA GAAATTACTT ATGTTTAAAA 
     2881 GAGTTCAGGA AGCAGAGGAT AAATGGAGGG GTGCGCAAGC CCTAATTGAG CAAATTAAAG 
     2941 CCACATTCTC AGAAAAGGAG AAAGAACTAG AAAATAAGTT GGAAGAACTA AAGAAACAAC 
     3001 AGGAAAAAGA ACTCTTCAAA TTGAATCAAG ATAATTATAT TCTTCAAGCC AAGTTAAGCA 
     3061 GCTTTGAAGA AACAAACAAA AAACAAAGGT GGTTACATTT TGGAGAAGCA GCTGATCCTG 
     3121 TCACTGGAGA AAAGTTGAAG CAAATCCAAA AAGAAATACA AGAACAAGAG ACACTTCTTC 
     3181 AAGGATATCA ACAGGAAAAT GAACGATTAT ATAATCAAGT AAAAGATCTC CAAGAACAAA 
     3241 ACAAGAAAAA TGAGGAGCGA ATGTTTAAGG AAAACCAAAG TTTATTCAGT GAGGTAGCTT 
     3301 CCTTAAAAGA ACAGATGCAC AAAAGTCGTT TTCTGTCTCA AGTAGTTGAA GATTCAGAGC 
     3361 CCACAAGAAA TCAGAATTTT ACAGATCTGT TAGCAGAACT ACGGATGGCA CAGAAAGAAA 
     3421 AAGACAGTTT ATTGGAAGAC ATCAAAAGAC TGAAACAAGA CAAACAAGCT CTTGAAGTAG 
     3481 ACTTCGAAAA AATGAAGAAA GAGAGGGACC AAGCCAAAGA TCAGATTGCT TATGTCACAG 
     3541 GTGAGAAATT ATATGAAATA AAAATTTTAG AAGAAACACA TAAACAAGAA ATCAGTCGTC 
     3601 TGCAAAAAAG ATTACAGTGG TATGCTGAAA ATCAGGAACT TCTGGATAAA GATGCACTTC 
     3661 GGCTTAGAGA AGCAAATGAG GAAATTGAGA AGCTCAAACT TGAGATTGAG AAACTGAAAG 
     3721 CTGAATCTGG GAATCCATCT ATTCGGCAGA AGATACGCTT AAAAGATAAA GCAGCAGATG 
     3781 CCAAAAAAAT TCAGGATCTG GAGCGACAAG TTAAGGAAAT GGAAGGGATT CTGAAGAGAA 
     3841 GATATCCCAA TTCTTTACCT GCTTTAATAT TGGCTGCATC AGCAGCTGGT GATACAGTGG 
     3901 ATAAAAATAC AGTGGAATTT ATGGAGAAAA GGATAAAAAA GCTAGAAGCT GATCTGGAGG 
     3961 GCAAAGATGA AGATGCAAAG AAAAGCCTTC GTACCATGGA ACAACAGTTT CAGAAAATGA 
     4021 AGATTCAGTA TGAACAAAGA CTAGAGCAGC AGGAGCAGCT ACTTGCCTGC AAATTGAATC 
     4081 AACATGACTC TCCCAGAATT AAAGCCCTAG AGAAGGAACT TGATGACATC AAGGAAGCCC 
     4141 ATCAGATCAC TGTAAGAAAC CTTGAAGCCG AAATAGACGT TCTTAAACAT CAGAATGCTG 
     4201 AATTAGACGT CAAGAAAAAT GATAAAGATG ATGAAGATTT TCAGTCTATA GAATTCCAGG 
     4261 TGGAACAGGC TCATGCTAAA GCTAAATTAG TAAGACTCAA TGAAGAACTG GCTGCAAAAA 
     4321 AGAGAGAAAT ACAAGACCTC TCAAAAACTG TGGAGAGGCT TCAGAAAGAC AGAAGAATGA 
     4381 TGCTATCAAA TCAGAACTCA AAGGGCAGAG AGGAAATGTC TGCCAAAAGG GCAAAGAAAG 
     4441 ATGTTTTGCA CTCAAGTAAA GGAAATGCTA ACTCCTTCCC TGGAACCCTG GACAGCAAGC 
     4501 TGTACCAACC ACATACTTTC ACTGATTCCC ATGTTTCAGA AGTTTTACAA GAAAACTACA 
     4561 GATTAAAAAA TGAGCTAGAA GGATTAATTT CAGAGAAGAA TGAACTGAAG ATGAAATCTG 
     4621 AAGCAGTGAT GAATCAATTT GAAAACTCCA TGAGAAGGGT CAAGGAAGAT ACTGCAGCAC 
     4681 ACATTGCATC CCTCAAAGCA TCTCATCAGA GAGAAATAGA GAAACTCCTT TGCCAAAATG 
     4741 CAGTAGAAAA TTCTTCTTCC AAAGTAGCTG AACTAAATCG TAAAATAGCA ACTCAAGAGG 
     4801 TACTTATAAG ACATTTCCAA AGTCAAGTTA ATGAGCTGCA GAGTAAACAA GAGTCTCTCG 
     4861 TAGTTTCTGA AGTTCGAGAA GAAATTCTAC AGAAAGAGAT TACAAAACTC TTAGAAGAAT 
     4921 TGAGAGAAGC CAAAGAAAAC CATACACCAG AGATGAAACA TTTCGTGGGC TTAGAAAAGA 
     4981 AAATTAAGCA GATGGAAATG AGACATGCAC AAAGAGAACA GGAACTTCAA CAGATAATAC 
     5041 AGCAAACACA CCAAGTAGTA GAAACTGAGC AAAACAAAGA AGTTGAAAAA TGGAAAAGAC 
     5101 TGGCACAGTT AAAGAATCGT GAGCTGGAGA AGTTCCGCAC AGAACTAGAC TCAATATTAG 
     5161 ATGTTCTCCG AGAGCTGCAC CGGCAAGGAG TGGTTGTGCC AGTTGCTTTT GCAGATGAAA 
     5221 TGAATGCACC AGAGTATACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5281 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5341 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5401 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5461 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5521 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5581 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5641 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5701 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5761 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5821 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5881 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5941 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6001 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6061 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6121 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6181 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6241 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6301 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6361 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6421 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6481 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6541 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6601 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6661 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6721 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6781 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6841 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6901 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6961 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7021 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7081 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7141 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7201 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7261 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7321 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7381 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7441 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7501 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7561 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7621 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7681 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7741 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7801 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7861 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7921 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7981 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8041 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8101 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8161 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8221 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8281 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8341 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8401 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8461 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8521 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8581 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8641 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8701 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8761 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8821 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8881 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8941 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9001 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9061 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//