LOCUS       RC217876                7149 bp    DNA     circular  SYN 
DEFINITION  LARGE (Myc-DDK-tagged)-Human like-glycosyltransferase (LARGE), transcript variant 1
ACCESSION   NM_004737
VERSION     NM_004737.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3306..3397 
                     /label="Myc-DDK"  
     misc_feature    3427..4012 
                     /label="PolyA signal"  
     misc_feature    3484..3503 
                     /label="Restriction Enzyme"  
     misc_feature    4288..4960 
                     /label="ColE1"  
     misc_feature    5390..6184 
                     /label="Kan/Neo"  
     misc_feature    6219..6521 
                     /label="SV40 ori"  
     misc_feature    6607..7084 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3288..3294 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3288 
                     /label="LARGE"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTGGGAATC TGCAGGGGGA GACGGAAATT CTTGGCTGCC TCGTTGAGTC 
     1081 TTCTCTGCAT CCCAGCCATC ACCTGGATTT ACCTGTTTTC TGGGAGCTTC GAAGATGGAA 
     1141 AGCCCGTGTC TCTGTCACCG CTGGAGTCCC AGGCACACAG CCCCAGGTAC ACGGCCTCCA 
     1201 GCCAGCGGGA GCGCGAGAGC CTGGAGGTGC GCATGCGCGA GGTGGAGGAG GAGAACCGCG 
     1261 CCCTCCGCAG GCAGCTCAGC CTGGCCCAGG GCCGAGCCCC ATCCCATCGC CGAGGCAACC 
     1321 ACTCCAAGAC CTACTCCATG GAGGAGGGCA CTGGAGACAG CGAGAACCTT CGGGCTGGCA 
     1381 TCGTGGCAGG CAACAGCTCC GAGTGTGGGC AGCAGCCGGT CGTGGAGAAA TGCGAGACAA 
     1441 TCCACGTTGC TATTGTCTGC GCCGGATACA ATGCCAGCCG GGATGTCGTC ACCCTGGTCA 
     1501 AATCCGTCCT GTTCCATAGA CGGAACCCTC TGCACTTCCA CCTTATTGCT GACTCCATTG 
     1561 CGGAGCAGAT CCTGGCCACG CTCTTCCAGA CCTGGATGGT GCCCGCTGTG CGTGTGGACT 
     1621 TCTACAATGC AGACGAGCTC AAGTCTGAAG TTTCCTGGAT CCCCAATAAA CATTACTCTG 
     1681 GGATTTATGG TCTGATGAAG CTTGTCCTGA CCAAGACTCT TCCTGCCAAC CTGGAGAGAG 
     1741 TCATCGTCCT TGACACGGAT ATCACCTTTG CCACTGACAT TGCAGAGCTG TGGGCTGTGT 
     1801 TCCACAAGTT CAAAGGTCAG CAAGTCCTGG GCTTGGTGGA GAACCAGAGT GACTGGTACC 
     1861 TTGGAAACCT GTGGAAAAAT CACCGCCCAT GGCCAGCCCT TGGAAGAGGC TACAACACAG 
     1921 GGGTGATCCT GTTACTTCTG GATAAGCTGC GGAAGATGAA ATGGGAGCAG ATGTGGAGGC 
     1981 TGACCGCAGA GAGGGAGCTC ATGGGCATGC TCTCTACATC CTTAGCTGAC CAGGATATTT 
     2041 TCAATGCCGT CATCAAACAA AACCCCTTCC TTGTGTACCA GCTCCCCTGC TTCTGGAATG 
     2101 TGCAGCTGTC AGACCACACC CGCTCCGAGC AGTGCTACAG AGACGTGTCT GATCTAAAGG 
     2161 TCATTCACTG GAACTCCCCC AAGAAGCTCC GGGTGAAGAA CAAGCATGTG GAGTTTTTTC 
     2221 GCAACCTCTA CCTGACCTTC CTGGAGTATG ACGGCAATCT TCTGAGGCGG GAACTGTTTG 
     2281 GCTGCCCCAG TGAGGCTGAT GTCAACAGTG AAAACCTCCA GAAGCAGCTG TCTGAGCTGG 
     2341 ACGAGGACGA CCTGTGCTAT GAGTTCCGGC GAGAGCGCTT CACTGTCCAC CGCACCCACC 
     2401 TGTACTTCCT GCACTACGAG TATGAGCCTG CAGCAGACAG CACGGACGTC ACCCTGGTCG 
     2461 CTCAGCTGTC CATGGACAGG CTCCAGATGC TGGAGGCCAT CTGCAAGCAC TGGGAGGGGC 
     2521 CCATCAGCCT GGCCCTCTAC CTGTCAGACG CCGAGGCCCA GCAGTTCCTC CGCTACGCAC 
     2581 AGGGCTCTGA GGTGCTTATG AGCCGCCACA ACGTGGGCTA CCACATCGTG TACAAGGAGG 
     2641 GCCAGTTCTA CCCCGTGAAC CTGCTGCGCA ACGTGGCCAT GAAGCACATC AGCACTCCCT 
     2701 ACATGTTCCT GTCTGACATT GACTTCCTGC CCATGTATGG GCTCTATGAG TACCTCAGGA 
     2761 AGTCTGTCAT CCAGCTCGAT CTTGCCAACA CCAAGAAAGC AATGATTGTC CCCGCGTTCG 
     2821 AGACACTGCG CTACCGGCTG TCCTTCCCCA AGTCAAAAGC GGAGTTGCTG TCAATGCTGG 
     2881 ACATGGGGAC CCTCTTCACA TTCAGGTACC ACGTCTGGAC GAAAGGCCAC GCACCCACAA 
     2941 ACTTCGCCAA GTGGCGGACC GCCACCACGC CTTACCGGGT TGAGTGGGAG GCCGATTTTG 
     3001 AGCCGTATGT TGTTGTGAGA CGTGACTGCC CGGAGTACGA CCGGAGGTTT GTAGGCTTTG 
     3061 GCTGGAACAA AGTGGCTCAT ATCATGGAGC TGGATGTGCA GGAGTATGAG TTCATTGTGC 
     3121 TGCCCAACGC CTACATGATC CACATGCCTC ATGCCCCCAG CTTCGACATT ACCAAGTTCC 
     3181 GTTCCAACAA GCAATACCGC ATCTGTCTCA AAACCCTCAA GGAAGAGTTT CAGCAGGACA 
     3241 TGTCCCGCCG CTACGGCTTT GCTGCCCTGA AATATCTCAC AGCCGAGAAC AACAGCACGC 
     3301 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3361 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3421 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3481 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3541 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3601 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3661 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3721 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3781 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3841 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3901 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3961 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4021 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4141 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4621 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4921 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4981 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5041 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5101 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5161 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5221 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5281 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5341 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5401 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5461 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5521 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5581 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5641 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5701 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5761 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5821 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5881 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5941 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6001 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6061 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6121 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6181 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6241 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6301 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6361 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6421 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6481 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6541 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6601 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6661 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6721 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6781 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6841 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6901 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6961 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7021 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7081 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7141 GCGAATTTT
//