LOCUS       RC217782                8892 bp    DNA     circular  SYN 
DEFINITION  DROSHA (Myc-DDK-tagged)-Human drosha, ribonuclease type III (DROSHA), transcript variant 2
ACCESSION   NM_001100412
VERSION     NM_001100412.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5049..5140 
                     /label="Myc-DDK"  
     misc_feature    5170..5755 
                     /label="PolyA signal"  
     misc_feature    5227..5246 
                     /label="Restriction Enzyme"  
     misc_feature    6031..6703 
                     /label="ColE1"  
     misc_feature    7133..7927 
                     /label="Kan/Neo"  
     misc_feature    7962..8264 
                     /label="SV40 ori"  
     misc_feature    8350..8827 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5031..5037 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5031 
                     /label="DROSHA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGCAGGGA AACACATGTC ACAGAATGTC GTTCCACCCG GGACGAGGGT 
     1081 GTCCCCGAGG ACGAGGAGGA CATGGAGCCA GACCCTCAGC ACCATCCTTT AGGCCCCAAA 
     1141 ATCTGAGGCT GCTTCACCCT CAGCAGCCTC CTGTGCAATA TCAATATGAA CCTCCAAGTG 
     1201 CCCCTTCCAC CACTTTCTCA AACTCTCCAG CCCCCAATTT TCTCCCTCCA CGACCAGACT 
     1261 TTGTACCCTT CCCCCCACCC ATGCCTCCGT CAGCGCAAGG CCCTCTTCCC CCCTGCCCAA 
     1321 TCAGGCCGCC TTTCCCCAAC CACCAGATGA GGCACCCCTT CCCAGTTCCT CCTTGTTTTC 
     1381 CTCCCATGCC ACCACCAATG CCTTGTCCTA ATAACCCCCC AGTCCCTGGG GCACCTCCTG 
     1441 GACAAGGCAC TTTCCCCTTC ATGATGCCCC CTCCCTCCAT GCCTCATCCC CCGCCCCCTC 
     1501 CAGTCATGCC GCAGCAGGTT AATTATCAGT ACCCTCCGGG CTATTCTCAC CACAACTTCC 
     1561 CACCTCCCAG TTTTAATAGT TTCCAGAACA ACCCTAGTTC TTTCCTGCCC AGTGCTAATA 
     1621 ACAGCAGTAG TCCTCATTTC AGACATCTCC CTCCATACCC ACTCCCAAAG GCTCCCAGTG 
     1681 AGAGAAGGTC CCCAGAAAGG CTGAAACACT ATGATGACCA CAGGCACCGA GATCACAGTC 
     1741 ATGGGCGAGG TGAGAGGCAT CGGTCCCTGG ATCGGCGGGA GCGAGGCCGC AGTCCCGACA 
     1801 GGAGAAGACA AGACAGCCGG TACAGATCTG ATTATGACCG AGGGAGAACA CCATCTCGCC 
     1861 ACCGCAGCTA CGAACGGAGC AGAGAGCGAG AACGGGAGAG ACACAGGCAT CGAGACAACC 
     1921 GAAGATCACC ATCTCTGGAA AGGTCCTACA AAAAAGAGTA TAAGAGATCT GGAAGTCGCT 
     1981 CCCCAAGTAG GGAGAAGAAG AGAGCTCGTT GGGAGGAAGA AAAAGACCGT TGGAGTGACA 
     2041 ACCAGAGTTC TGGCAAAGAC AAGAACTATA CCTCAATCAA GGAAAAAGAG CCCGAGGAGA 
     2101 CCATGCCTGA CAAGAATGAG GAGGAAGAAG AAGAACTTCT TAAGCCTGTG TGGATTCGAT 
     2161 GCACTCATTC AGAAAACTAC TACTCCAGTG ACCCCATGGA TCAGGTGGGA GATTCTACAG 
     2221 TGGTTGGAAC GAGTAGGCTT CGTGACTTAT ATGACAAATT TGAGGAGGAG TTGGGGAGCA 
     2281 GGCAAGAAAA GGCCAAAGCT GCTCGGCCTC CGTGGGAACC TCCAAAGACG AAGCTCGATG 
     2341 AAGATTTAGA GAGTTCCAGT GAATCCGAGT GTGAGTCTGA TGAGGACAGC ACCTGTTCTA 
     2401 GCAGCTCAGA CTCTGAAGTT TTTGACGTTA TTGCAGAAAT CAAACGCAAA AAGGCCCACC 
     2461 CTGACCGACT TCATGATGAA CTTTGGTACA ACGATCCAGG CCAGATGAAT GATGGACCAC 
     2521 TCTGCAAATG CAGCGCAAAG GCAAGACGCA CAGGAATTAG GCACAGCATT TATCCTGGAG 
     2581 AAGAGGCCAT CAAGCCCTGT CGTCCTATGA CCAACAATGC TGGCAGACTT TTCCACTACC 
     2641 GGATCACAGT CTCCCCGCCT ACGAACTTTT TAACTGACAG GCCAACTGTT ATAGAATACG 
     2701 ATGATCACGA GTATATCTTT GAAGGATTTT CTATGTTTGC ACATGCCCCC CTGACCAATA 
     2761 TTCCACTGTG TAAAGTAATT AGATTCAACA TAGACTACAC GATTCATTTC ATTGAAGAGA 
     2821 TGATGCCGGA GAATTTTTGT GTGAAAGGGC TTGAACTCTT TTCACTGTTC CTATTCAGAG 
     2881 ATATTTTGGA ATTATATGAC TGGAATCTTA AAGGTCCTTT GTTTGAAGAC AGCCCTCCCT 
     2941 GCTGCCCAAG ATTTCATTTC ATGCCACGTT TTGTAAGATT TCTTCCAGAT GGAGGAAAGG 
     3001 AAGTGCTGTC CATGCACCAG ATTCTCCTGT ACTTGTTAAG GTGCAGCAAA GCCCTGGTGC 
     3061 CTGAGGAGGA GATTGCCAAT ATGCTTCAGT GGGAGGAGCT GGAGTGGCAG AAATATGCAG 
     3121 AAGAATGCAA AGGCATGATT GTTACCAACC CTGGGACGAA ACCAAGCTCT GTCCGTATCG 
     3181 ATCAACTGGA TCGTGAACAG TTCAACCCCG ATGTGATTAC TTTTCCGATT ATCGTCCACT 
     3241 TTGGGATACG CCCTGCACAG TTGAGTTATG CAGGAGACCC ACAGTACCAA AAACTGTGGA 
     3301 AGAGTTATGT GAAACTTCGC CACCTCCTAG CAAATAGTCC CAAAGTCAAA CAAACTGACA 
     3361 AACAGAAGCT GGCACAGAGG GAGGAAGCCC TCCAAAAAAT ACGGCAGAAG AATACAATGA 
     3421 GACGAGAAGT AACGGTGGAG CTAAGTAGCC AAGGATTCTG GAAAACTGGC ATCCGTTCTG 
     3481 ATGTCTGTCA GCATGCAATG ATGCTACCTG TTCTGACCCA TCATATCCGC TACCACCAAT 
     3541 GCCTAATGCA TTTGGACAAG TTGATAGGAT ATACTTTCCA AGATCGTTGT CTGTTGCAGC 
     3601 TGGCCATGAC TCATCCAAGT CATCATTTAA ATTTTGGAAT GAATCCTGAT CATGCCAGGA 
     3661 ATTCATTATC TAACTGTGGA ATTCGGCAGC CCAAATACGG AGACAGAAAA GTTCATCACA 
     3721 TGCACATGCG GAAGAAAGGG ATTAACACCT TGATAAATAT CATGTCACGC CTTGGCCAAG 
     3781 ATGACCCAAC TCCCTCGAGG ATTAACCACA ATGAACGGTT GGAATTCCTG GGTGATGCTG 
     3841 TTGTTGAATT TCTGACCAGC GTCCATTTGT ACTATTTGTT TCCTAGTCTG GAAGAAGGAG 
     3901 GATTAGCAAC CTATCGGACT GCCATTGTTC AGAATCAGCA CCTTGCCATG CTAGCAAAGA 
     3961 AACTTGAACT GGATCGATTT ATGCTGTATG CTCACGGGCC TGACCTTTGT AGAGAATCGG 
     4021 ACCTTCGACA TGCAATGGCC AATTGTTTTG AAGCGTTAAT AGGAGCTGTT TACTTGGAGG 
     4081 GAAGCCTGGA GGAAGCCAAG CAGTTATTTG GACGCTTGCT CTTTAATGAT CCGGACCTGC 
     4141 GCGAAGTCTG GCTCAATTAT CCTCTCCACC CACTCCAACT ACAAGAGCCA AATACTGATC 
     4201 GACAACTTAT TGAAACTTCT CCAGTTCTAC AAAAACTTAC TGAGTTTGAA GAAGCAATTG 
     4261 GAGTAATTTT TACTCATGTT CGACTTCTGG CAAGGGCATT CACATTGAGA ACTGTGGGAT 
     4321 TTAACCATCT GACCCTAGGC CACAATCAGA GAATGGAATT CCTAGGTGAC TCCATAATGC 
     4381 AACTGGTAGC CACAGAGTAC TTATTCATTC ATTTCCCAGA TCATCATGAA GGACACTTAA 
     4441 CTTTGTTGCG AAGCTCTTTG GTGAATAATA GAACTCAGGC CAAGGTAGCG GAGGAGCTGG 
     4501 GCATGCAGGA GTACGCCATA ACCAACGACA AGACCAAGAG GCCTGTGGCG CTTCGCACCA 
     4561 AGACCTTGGC GGACCTTTTG GAATCATTTA TTGCAGCGCT GTACATTGAT AAGGATTTGG 
     4621 AATATGTTCA TACTTTCATG AATGTCTGCT TCTTTCCACG ATTGAAAGAG TTCATTTTGA 
     4681 ATCAGGATTG GAATGACCCC AAATCCCAGC TTCAGCAGTG TTGCTTGACA CTTAGGACAG 
     4741 AAGGAAAAGA GCCAGACATT CCTCTGTACA AGACTCTGCA GACAGTGGGC CCATCCCATG 
     4801 CCCGAACCTA CACTGTGGCT GTTTATTTCA AGGGAGAAAG AATAGGCTGT GGGAAAGGAC 
     4861 CAAGTATTCA GCAAGCGGAA ATGGGAGCAG CAATGGATGC GCTTGAAAAA TATAATTTTC 
     4921 CCCAGATGGC CCATCAGAAG CGGTTCATCG AACGGAAGTA CAGACAAGAG TTAAAAGAAA 
     4981 TGAGGTGGGA AAGAGAGCAT CAAGAGAGAG AGCCAGATGA GACTGAAGAC ATCAAGAAAA 
     5041 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5101 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5161 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5221 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5281 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5341 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5401 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5461 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5521 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5581 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5641 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5701 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5761 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5821 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5881 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5941 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6001 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6061 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6121 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6181 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6241 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6301 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6361 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6421 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6481 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6541 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6601 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6661 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6721 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6781 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6841 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6901 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6961 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7021 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7081 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7141 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7201 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7261 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7321 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7381 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7441 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7501 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7561 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7621 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7681 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7741 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7801 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7861 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7921 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7981 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8041 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8101 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8161 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8221 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8281 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8341 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8401 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8461 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8521 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8581 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8641 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8701 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8761 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8821 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8881 AACGCGAATT TT
//