LOCUS       RC217755                8445 bp    DNA     circular  SYN 
DEFINITION  PTPRO (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, O (PTPRO), transcript variant 2
ACCESSION   NM_002848
VERSION     NM_002848.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4602..4693 
                     /label="Myc-DDK"  
     misc_feature    4723..5308 
                     /label="PolyA signal"  
     misc_feature    4780..4799 
                     /label="Restriction Enzyme"  
     misc_feature    5584..6256 
                     /label="ColE1"  
     misc_feature    6686..7480 
                     /label="Kan/Neo"  
     misc_feature    7515..7817 
                     /label="SV40 ori"  
     misc_feature    7903..8380 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4584..4590 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4584 
                     /label="PTPRO"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCACCTG CCCACGGGGA TACACGGCGC CCGCCGCCTC CTGCCTCTGC 
     1081 TCTGGCTCTT TGTGCTGTTC AAGAATGCTA CAGCTTTCCA TGTAACTGTC CAAGATGATA 
     1141 ATAACATCGT TGTCTCATTA GAAGCTTCAG ACGTCATCAG TCCAGCATCT GTGTATGTTG 
     1201 TGAAGATAAC TGGTGAATCC AAAAATTATT TCTTCGAATT TGAGGAATTC AACAGCACTT 
     1261 TGCCTCCTCC TGTTATTTTC AAGGCCAGTT ATCATGGCCT TTATTATATA ATCACTCTGG 
     1321 TAGTGGTAAA TGGAAATGTG GTGACCAAGC CATCCAGATC AATCACTGTG TTAACAAAAC 
     1381 CTCTACCTGT AACCAGTGTT TCCATATATG ACTATAAACC TTCTCCTGAA ACAGGAGTCC 
     1441 TGTTTGAAAT ACATTATCCA GAAAAATATA ACGTTTTCAC AAGAGTGAAC ATTAGCTACT 
     1501 GGGAAGGTAA AGACTTCCGG ACAATGCTAT ATAAAGATTT CTTTAAGGGA AAAACAGTAT 
     1561 TTAATCACTG GCTGCCAGGA ATGTGTTATA GTAATATCAC CTTTCAGCTG GTATCTGAGG 
     1621 CAACTTTTAA TAAAAGTACC CTTGTTGAGT ACAGTGGTGT CAGTCACGAA CCCAAACAGC 
     1681 ACAGAACTGC CCCTTATCCA CCTCAAAATA TTTCCGTTCG TATCGTAAAC TTGAACAAAA 
     1741 ACAACTGGGA AGAACAGAGT GGCAATTTCC CAGAAGAATC CTTCATGAGA TCACAAGATA 
     1801 CAATAGGAAA AGAAAAACTC TTCCATTTTA CAGAAGAAAC CCCTGAAATT CCCTCGGGCA 
     1861 ACATTTCTTC CGGTTGGCCT GATTTTAATA GCAGTGACTA TGAAACTACG TCTCAGCCAT 
     1921 ATTGGTGGGA CAGTGCATCT GCAGCTCCTG AAAGTGAAGA TGAATTTGTC AGCGTACTTC 
     1981 CCATGGAATA CGAAAATAAC AGTACACTCA GTGAGACAGA GAAGTCAACA TCAGGCTCTT 
     2041 TCTCCTTTTT CCCTGTGCAA ATGATATTGA CCTGGTTACC ACCCAAACCA CCCACTGCTT 
     2101 TTGATGGGTT CCATATCCAT ATTGAACGAG AAGAGAACTT TACTGAATAT TTGATGGTGG 
     2161 ATGAAGAAGC ACATGAATTT GTTGCAGAAC TGAAGGAACC TGGGAAATAT AAGTTATCTG 
     2221 TGACAACCTT TAGTTCCTCA GGATCTTGTG AAACTCGAAA AAGTCAGTCA GCAAAATCAC 
     2281 TCAGCTTTTA TATCAGTCCT TCAGGAGAGT GGATTGAAGA ACTGACCGAG AAGCCGCAGC 
     2341 ACGTGAGTGT CCACGTTTTA AGCTCAACCA CTGCCTTGAT GTCCTGGACA TCTTCCCAAG 
     2401 AGAACTACAA CAGCACCATT GTGTCTGTGG TGTCGCTGAC CTGCCAGAAA CAAAAGGAGA 
     2461 GCCAGAGGCT TGAAAAGCAG TACTGCACTC AGGTGAACTC AAGCAAACCT ATTATTGAAA 
     2521 ATCTGGTTCC TGGTGCCCAG TACCAGGTTG TAATATACCT AAGGAAAGGC CCTTTGATTG 
     2581 GACCACCTTC AGATCCTGTG ACATTTGCTA TTGTTCCCAC AGGAATAAAG GATTTAATGC 
     2641 TCTATCCTTT GGGTCCTACG GCCGTGGTTC TGAGCTGGAC CAGACCTTAT TTAGGCGTGT 
     2701 TCAGAAAATA CGTGGTTGAA ATGTTTTATT TCAACCCTGC TACAATGACA TCAGAGTGGA 
     2761 CCACCTACTA TGAAATAGCA GCAACTGTTT CCTTAACTGC ATCCGTGAGA ATAGCTAATC 
     2821 TGCTGCCAGC ATGGTACTAC AACTTCCGGG TTACCATGGT GACGTGGGGA GATCCAGAAT 
     2881 TGAGCTGCTG TGACAGCTCT ACCATCAGCT TCATAACAGC CCCAGTGGCT CCGGAAATCA 
     2941 CTTCTGTGGA ATATTTCAAC AGTCTGTTAT ATATCAGTTG GACATATGGG GATGATACAA 
     3001 CGGACTTGTC CCATTCTAGA ATGCTTCACT GGATGGTGGT TGCAGAAGGA AAAAAGAAAA 
     3061 TTAAAAAGAG TGTAACACGC AATGTCATGA CTGCAATTCT CAGCTTGCCT CCAGGCGACA 
     3121 TCTATAACCT CTCAGTAACT GCTTGTACTG AAAGAGGAAG TAATACCTCC ATGCTCCGCC 
     3181 TTGTCAAGCT AGAACCAGCT CCACCCAAAT CACTCTTCGC AGTGAACAAA ACCCAGACTT 
     3241 CAGTGACTTT GCTGTGGGTG GAAGAGGGAG TAGCTGATTT CTTTGAAGTT TTCTGTCAAC 
     3301 AAGTTGGCTC CAGTCAGAAA ACCAAACTTC AGGAACCAGT TGCTGTTTCT TCCCATGTCG 
     3361 TGACCATCTC CAGCCTTCTT CCTGCCACTG CCTACAATTG TAGTGTCACC AGCTTTAGCC 
     3421 ATGACAGCCC CAGTGTCCCT ACGTTCATAG CCGTCTCAAC AATGGTTACA GAGATGAATC 
     3481 CCAATGTGGT AGTGATCTCC GTGCTGGCCA TCCTTAGCAC ACTTTTAATT GGACTGTTGC 
     3541 TTGTTACCCT CATTATTCTT AGGAAAAAGC ATCTGCAGAT GGCTAGGGAG TGTGGAGCTG 
     3601 GTACATTTGT CAATTTTGCA TCCTTAGAGA GGGATGGAAA GCTTCCATAC AACTGGAGTA 
     3661 AAAATGGTTT AAAGAAGAGG AAACTGACAA ACCCGGTTCA ACTGGATGAC TTTGATGCCT 
     3721 ATATTAAGGA TATGGCCAAA GACTCTGACT ATAAATTTTC TCTTCAGTTT GAGGAGTTGA 
     3781 AATTGATTGG ACTGGATATC CCACACTTTG CTGCAGATCT TCCACTGAAT CGATGTAAAA 
     3841 ACCGTTACAC AAACATCCTA CCATATGACT TCAGCCGTGT GAGATTAGTC TCCATGAATG 
     3901 AAGAGGAAGG TGCAGACTAC ATCAATGCCA ACTATATTCC TGGATACAAC TCACCCCAGG 
     3961 AGTATATTGC CACCCAGGGG CCACTGCCTG AAACCAGAAA TGACTTCTGG AAGATGGTCC 
     4021 TGCAACAAAA GTCTCAGATT ATTGTCATGC TCACTCAGTG TAATGAGAAA AGGAGGGTGA 
     4081 AATGTGACCA TTACTGGCCA TTCACGGAAG AACCTATAGC CTATGGAGAC ATCACTGTGG 
     4141 AGATGATTTC AGAGGAAGAG CAGGACGACT GGGCCTGTAG ACACTTCCGG ATCAACTATG 
     4201 CTGACGAGAT GCAGGATGTG ATGCATTTTA ACTACACTGC ATGGCCTGAT CATGGTGTGC 
     4261 CCACAGCAAA TGCTGCAGAA AGTATCCTGC AGTTTGTACA CATGGTCCGA CAGCAAGCTA 
     4321 CCAAGAGCAA AGGTCCCATG ATCATTCACT GCAGTGCTGG CGTGGGACGG ACAGGAACAT 
     4381 TCATTGCCCT GGACAGGCTC TTGCAGCACA TTCGGGATCA TGAGTTTGTT GACATCTTAG 
     4441 GGCTGGTGTC AGAAATGAGG TCATACCGGA TGTCTATGGT ACAGACAGAG GAGCAGTACA 
     4501 TTTTTATCCA TCAGTGTGTG CAACTGATGT GGATGAAGAA GAAGCAGCAG TTCTGCATCA 
     4561 GTGATGTCAT ATACGAGAAT GTTAGCAAGT CCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4621 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4681 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4741 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4801 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4861 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4921 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4981 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5041 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5101 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5161 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5221 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5281 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5341 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5401 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5461 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5521 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5581 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5641 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5701 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5761 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5821 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5881 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5941 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6001 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6061 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6121 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6181 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6241 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6301 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6361 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6421 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6481 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6541 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6601 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6661 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6721 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6781 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6841 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6901 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6961 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7021 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7081 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7141 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7201 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7261 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7321 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7381 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7441 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7501 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7561 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7621 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7681 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7801 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7861 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7921 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7981 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8041 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8101 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8161 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8221 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8281 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8341 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8401 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//