LOCUS RC217750 7119 bp DNA circular SYN DEFINITION CLCN5 (Myc-DDK-tagged)-Human chloride channel 5 (CLCN5), transcript variant 3 ACCESSION NM_000084 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3276..3367 /label="Myc-DDK" misc_feature 3397..3982 /label="PolyA signal" misc_feature 3454..3473 /label="Restriction Enzyme" misc_feature 4258..4930 /label="ColE1" misc_feature 5360..6154 /label="Kan/Neo" misc_feature 6189..6491 /label="SV40 ori" misc_feature 6577..7054 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3258..3264 /label="Restriction Enzyme" misc_feature 1020..3258 /label="CLCN5" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGACTTCTTG GAGGAGCCAA TCCCTGGTGT AGGGACCTAT GATGATTTCA 1081 ATACAATTGA TTGGGTGAGA GAGAAGTCTC GAGACCGGGA TAGGCACCGA GAGATTACCA 1141 ATAAAAGCAA AGAGTCAACA TGGGCCTTAA TTCACAGTGT GAGTGATGCT TTTTCCGGCT 1201 GGTTGTTGAT GCTCCTTATT GGGCTTTTAT CAGGTTCGTT AGCTGGTTTG ATAGACATCT 1261 CTGCTCATTG GATGACAGAC TTAAAAGAAG GTATATGCAC AGGGGGATTC TGGTTTAACC 1321 ATGAACATTG TTGCTGGAAC TCTGAGCATG TCACCTTTGA AGAGAGAGAC AAATGTCCAG 1381 AGTGGAATAG TTGGTCCCAG CTTATCATCA GCACAGATGA GGGAGCCTTT GCCTACATAG 1441 TCAATTATTT CATGTACGTC CTCTGGGCTC TCCTATTTGC CTTCCTTGCC GTATCTCTTG 1501 TCAAGGTGTT TGCGCCTTAT GCCTGTGGCT CTGGAATCCC TGAGATAAAA ACTATCTTGA 1561 GTGGTTTCAT TATTAGGGGC TATTTGGGTA AGTGGACTCT GGTTATCAAA ACCATCACCT 1621 TGGTGCTGGC AGTGTCGTCT GGCTTGAGCC TGGGCAAAGA GGGCCCTCTA GTGCACGTGG 1681 CTTGCTGCTG TGGGAACATC CTGTGCCACT GCTTCAACAA ATACAGGAAG AATGAAGCCA 1741 AGCGCAGAGA GGTCTTGTCG GCTGCAGCAG CAGCTGGTGT ATCTGTAGCC TTTGGAGCAC 1801 CTATAGGTGG AGTATTATTC AGCCTTGAAG AGGTCAGCTA CTATTTTCCC CTCAAAACAT 1861 TGTGGCGTTC ATTCTTTGCT GCCTTGGTGG CAGCATTCAC TCTACGCTCC ATCAATCCAT 1921 TTGGGAACAG CCGCCTGGTA CTATTTTATG TGGAGTTTCA CACCCCATGG CATCTCTTTG 1981 AGCTCGTGCC ATTCATTCTG CTGGGCATAT TTGGTGGTCT GTGGGGAGCA CTGTTTATCC 2041 GCACAAACAT TGCCTGGTGT CGGAAGCGAA AGACCACCCA GTTGGGCAAG TATCCTGTTA 2101 TAGAGGTACT CGTCGTGACA GCCATCACTG CCATCCTGGC TTTCCCCAAT GAATACACTC 2161 GGATGAGCAC AAGTGAGCTC ATTTCTGAGC TGTTTAATGA CTGTGGCCTT CTGGACTCCT 2221 CCAAGCTCTG TGATTATGAG AACCGTTTCA ACACAAGCAA AGGGGGTGAA CTGCCTGACA 2281 GACCGGCTGG CGTGGGAGTC TACAGTGCAA TGTGGCAGCT GGCTTTAACA CTCATACTGA 2341 AAATTGTCAT TACTATATTC ACCTTTGGCA TGAAGATCCC TTCTGGCCTC TTTATCCCTA 2401 GCATGGCTGT TGGTGCTATA GCAGGTCGAC TTCTAGGAGT AGGAATGGAA CAGCTGGCTT 2461 ATTACCACCA GGAATGGACC GTCTTCAATA GCTGGTGTAG TCAGGGAGCT GATTGCATCA 2521 CCCCCGGCCT TTATGCAATG GTTGGGGCTG CAGCCTGCTT AGGTGGGGTG ACTCGGATGA 2581 CTGTTTCTCT TGTTGTCATA ATGTTTGAAC TGACTGGTGG CTTAGAATAC ATCGTGCCTC 2641 TGATGGCTGC AGCCATGACA AGCAAGTGGG TGGCAGATGC TCTTGGGCGG GAGGGCATCT 2701 ATGATGCCCA CATCCGTCTC AATGGATACC CCTTTCTTGA AGCCAAAGAA GAGTTTGCTC 2761 ATAAGACCCT GGCAATGGAT GTGATGAAAC CCCGGAGAAA TGATCCTTTG TTGACTGTCC 2821 TTACTCAGGA CAGTATGACT GTGGAAGATG TAGAGACCAT AATCAGTGAA ACCACTTACA 2881 GTGGCTTCCC AGTGGTGGTA TCCCGGGAGT CCCAAAGACT TGTGGGCTTT GTCCTCCGAA 2941 GAGATCTCAT TATTTCAATT GAAAATGCTC GAAAGAAACA GGATGGGGTT GTTAGCACTT 3001 CCATCATTTA TTTCACGGAG CATTCTCCTC CATTGCCACC ATACACTCCA CCCACTCTAA 3061 AGCTTCGGAA CATCCTCGAT CTCAGCCCCT TCACTGTGAC TGACCTTACA CCCATGGAGA 3121 TCGTAGTGGA TATTTTCCGA AAGCTGGGAC TGCGGCAGTG CCTGGTTACA CACAACGGGC 3181 GATTGCTTGG AATCATTACC AAAAAGGATG TGTTAAAGCA TATAGCACAG ATGGCGAACC 3241 AAGATCCTGA TTCCATTCTC TTCAACACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 3301 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 3361 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 3421 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 3481 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 3541 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 3601 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 3661 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 3721 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 3781 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 3841 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 3901 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 3961 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 4021 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 4081 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 4141 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 4201 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 4261 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 4321 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 4381 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 4441 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 4501 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 4561 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 4621 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 4681 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 4741 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 4801 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 4861 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 4921 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 4981 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 5041 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 5101 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 5161 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 5221 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 5281 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 5341 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 5401 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 5461 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 5521 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 5581 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 5641 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 5701 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 5761 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 5821 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 5881 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 5941 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 6001 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 6061 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 6121 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 6181 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 6241 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 6301 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 6361 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 6421 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 6481 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 6541 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 6601 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 6661 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 6721 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 6781 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 6841 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 6901 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 6961 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 7021 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 7081 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT //