LOCUS       RC217692                8370 bp    DNA     circular  SYN 
DEFINITION  AFF4 (Myc-DDK-tagged)-Human AF4/FMR2 family, member 4 (AFF4)
ACCESSION   NM_014423
VERSION     NM_014423.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4527..4618 
                     /label="Myc-DDK"  
     misc_feature    4648..5233 
                     /label="PolyA signal"  
     misc_feature    4705..4724 
                     /label="Restriction Enzyme"  
     misc_feature    5509..6181 
                     /label="ColE1"  
     misc_feature    6611..7405 
                     /label="Kan/Neo"  
     misc_feature    7440..7742 
                     /label="SV40 ori"  
     misc_feature    7828..8305 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4509..4515 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4509 
                     /label="AFF4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACCGTGAA GACCGGAATG TGCTGCGTAT GAAAGAACGG GAAAGGCGGA 
     1081 ATCAGGAAAT TCAGCAGGGC GAAGACGCCT TCCCACCTAG CTCTCCTCTC TTTGCAGAGC 
     1141 CATACAAAGT TACTAGCAAA GAAGATAAGT TATCAAGTCG TATTCAGAGT ATGCTTGGAA 
     1201 ACTACGATGA AATGAAGGAT TTCATAGGAG ACAGATCTAT ACCAAAGCTT GTTGCAATTC 
     1261 CCAAGCCTAC AGTACCACCA TCAGCAGATG AAAAATCTAA CCCAAATTTC TTTGAACAGA 
     1321 GACATGGAGG CTCTCATCAG AGTAGCAAAT GGACTCCAGT AGGACCCGCA CCCAGCACTT 
     1381 CTCAGTCTCA GAAACGGTCC TCAGGCTTAC AGAGTGGACA TAGTAGCCAG CGGACCAGCG 
     1441 CAGGTAGCAG TAGTGGCACT AACAGTAGTG GTCAGAGGCA CGACCGTGAG TCATATAACA 
     1501 ATAGTGGGAG CAGTAGCCGG AAAAAAGGCC AGCATGGATC AGAACACTCC AAATCACGTT 
     1561 CTTCCAGCCC TGGAAAACCC CAGGCTGTTT CTTCATTAAA CTCTAGTCAT TCCAGGTCTC 
     1621 ATGGGAATGA TCACCATAGC AAGGAACATC AACGCTCCAA ATCACCTCGG GACCCTGATG 
     1681 CAAACTGGGA TTCTCCTTCC CGTGTACCTT TTTCAAGTGG GCAGCACTCA ACTCAATCTT 
     1741 TCCCACCCTC ATTGATGTCA AAGTCCAATT CAATGTTACA GAAACCCACT GCCTATGTGC 
     1801 GGCCCATGGA CGGACAGGAG TCCATGGAAC CAAAGCTGTC CTCTGAGCAC TACAGCAGCC 
     1861 AATCCCATGG CAACAGCATG ACTGAGCTGA AGCCCAGCAG CAAAGCACAT CTCACCAAGC 
     1921 TGAAAATACC TTCCCAACCA CTGGATGCAT CAGCTTCTGG TGATGTGAGC TGTGTGGATG 
     1981 AAATCCTAAA AGAGATGACG CATTCATGGC CTCCCCCTCT AACGGCTATT CATACACCAT 
     2041 GCAAAACAGA ACCTTCCAAA TTTCCTTTTC CAACTAAGGA GTCTCAGCAG TCCAATTTTG 
     2101 GCACTGGAGA ACAAAAAAGA TATAATCCTT CTAAAACTTC AAATGGGCAC CAGTCTAAAT 
     2161 CTATGTTAAA AGATGACTTA AAACTAAGCA GCAGTGAAGA CAGTGATGGG GAACAGGATT 
     2221 GTGATAAGAC AATGCCGAGG AGTACACCAG GAAGTAACTC TGAACCTTCA CACCATAATA 
     2281 GTGAAGGAGC AGATAACTCC AGGGATGATT CTAGTAGCCA CAGTGGATCT GAAAGCAGCT 
     2341 CTGGATCTGA CTCAGAGAGT GAAAGTAGTT CCAGTGACAG TGAGGCAAAT GAGCCATCCC 
     2401 AGAGTGCATC TCCCGAGCCT GAACCCCCGC CAACAAACAA ATGGCAACTT GATAATTGGC 
     2461 TGAATAAAGT GAACCCACAT AAAGTGTCAC CCGCCTCTTC AGTGGACAGT AACATCCCAT 
     2521 CATCTCAAGG CTACAAAAAG GAAGGCCGAG AGCAGGGCAC TGGGAATAGC TACACTGATA 
     2581 CAAGTGGACC TAAAGAAACG AGTTCCGCTA CTCCGGGACG AGACTCCAAA ACCATCCAAA 
     2641 AGGGATCAGA AAGTGGGCGT GGGAGGCAGA AATCTCCTGC ACAGAGTGAC AGCACAACAC 
     2701 AGAGAAGAAC TGTAGGCAAA AAACAACCCA AAAAGGCTGA GAAGGCAGCT GCTGAAGAGC 
     2761 CTCGTGGAGG CCTGAAGATA GAAAGTGAAA CCCCTGTAGA CTTGGCTAGC AGCATGCCCT 
     2821 CCAGCAGACA CAAAGCAGCC ACCAAAGGCT CAAGGAAACC CAATATAAAG AAGGAGTCTA 
     2881 AGTCTTCCCC TCGACCTACA GCAGAGAAAA AGAAATATAA GTCAACAAGT AAATCTTCCC 
     2941 AGAAATCAAG GGAAATCATA GAAACAGATA CCTCATCCTC AGATTCAGAT GAAAGTGAGA 
     3001 GCCTTCCTCC TTCCTCACAA ACTCCTAAGT ACCCCGAGAG CAATAGGACT CCTGTTAAAC 
     3061 CCTCCTCAGT GGAGGAAGAA GATAGCTTTT TTCGGCAACG AATGTTCTCT CCTATGGAAG 
     3121 AGAAGGAACT TCTTTCACCC CTCAGTGAGC CTGATGACAG GTACCCACTT ATTGTGAAGA 
     3181 TTGACCTGAA TCTTTTGACT AGAATACCAG GAAAGCCTTA CAAAGAAACA GAGCCGCCCA 
     3241 AGGGGGAAAA GAAAAATGTG CCAGAAAAGC ACACGAGAGA GGCTCAGAAA CAAGCCTCAG 
     3301 AAAAAGTTTC CAACAAAGGC AAGAGGAAGC ATAAGAATGA AGATGATAAC CGAGCCAGTG 
     3361 AGAGCAAGAA ACCCAAAACG GAGGACAAGA ATTCAGCAGG CCATAAGCCA TCCAGCAACA 
     3421 GAGAGTCATC TAAGCAGAGT GCTGCAAAAG AAAAGGATTT GTTGCCTTCT CCCGCTGGGC 
     3481 CTGTTCCTTC AAAAGATCCA AAAACAGAGC ATGGCTCTCG GAAGAGGACT ATTAGTCAGT 
     3541 CTTCTTCCTT AAAGTCAAGC AGTAACAGCA ACAAGGAGAC GAGTGGCAGC AGCAAAAACA 
     3601 GTTCCTCCAC ATCAAAGCAG AAGAAGACCG AAGGGAAGAC TTCCAGTAGC TCCAAGGAGG 
     3661 TTAAGGAAAA GGCTCCAAGT AGCTCCTCTA ACTGTCCTCC ATCTGCACCA ACTCTTGATT 
     3721 CTTCTAAGCC TCGGAGAACA AAGCTTGTCT TTGATGACAG AAATTATTCA GCAGACCATT 
     3781 ATTTACAAGA AGCAAAAAAG CTAAAGCACA ATGCAGATGC ATTGTCTGAT AGGTTTGAGA 
     3841 AAGCTGTATA CTATCTTGAT GCTGTGGTAT CTTTCATTGA ATGTGGGAAT GCATTAGAGA 
     3901 AGAATGCTCA GGAATCCAAA TCCCCATTCC CTATGTATTC AGAGACGGTG GATCTCATCA 
     3961 AATACACTAT GAAGCTAAAG AATTACTTGG CACCAGATGC TACAGCTGCA GATAAACGAC 
     4021 TCACAGTACT TTGCCTGCGA TGCGAGTCTT TGCTGTACCT GAGGCTGTTC AAACTGAAGA 
     4081 AGGAAAATGC TCTGAAGTAC TCAAAGACAC TGACAGAGCA CCTGAAGAAT TCTTATAATA 
     4141 ATTCTCAAGC ACCATCGCCT GGCTTGGGAA GCAAAGCTGT GGGGATGCCT TCCCCTGTTT 
     4201 CTCCAAAGCT GTCACCAGGC AATTCAGGAA ATTATTCATC TGGGGCCAGT AGTGCTTCTG 
     4261 CAAGTGGTTC TTCAGTGACC ATTCCACAGA AGATCCACCA GATGGCAGCC AGCTATGTTC 
     4321 AGGTCACATC CAACTTCCTC TATGCCACCG AAATTTGGGA CCAAGCTGAA CAGCTTTCCA 
     4381 AAGAGCAAAA AGAATTCTTT GCTGAACTGG ATAAAGTAAT GGGCCCTCTC ATCTTTAATG 
     4441 CAAGCATCAT GACAGATCTA GTTCGTTATA CCCGGCAGGG ACTGCACTGG CTTCGCCAGG 
     4501 ATGCCAAGTT GATATCTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4561 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4621 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4681 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4741 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4801 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4861 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4921 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4981 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5041 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5101 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     5161 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     5221 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     5281 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     5341 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5401 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5461 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5521 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5581 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5641 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5701 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5761 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5821 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5881 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5941 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6001 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6061 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6121 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     6181 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     6241 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     6301 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6361 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6421 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6481 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6541 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6601 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6661 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6721 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6781 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6841 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6901 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6961 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7021 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7081 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7141 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     7201 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     7261 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     7321 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7381 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7441 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7501 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7561 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7621 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7681 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7741 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7801 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7861 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7921 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7981 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8041 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8101 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     8161 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     8221 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     8281 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     8341 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//