LOCUS       RC217661                8472 bp    DNA     circular  SYN 
DEFINITION  SMC2 (Myc-DDK-tagged)-Human structural maintenance of chromosomes 2 (SMC2), transcript variant 3
ACCESSION   NM_006444
VERSION     NM_006444.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4629..4720 
                     /label="Myc-DDK"  
     misc_feature    4750..5335 
                     /label="PolyA signal"  
     misc_feature    4807..4826 
                     /label="Restriction Enzyme"  
     misc_feature    5611..6283 
                     /label="ColE1"  
     misc_feature    6713..7507 
                     /label="Kan/Neo"  
     misc_feature    7542..7844 
                     /label="SV40 ori"  
     misc_feature    7930..8407 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4611..4617 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4611 
                     /label="SMC2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATATTAAG TCAATTATTC TAGAGGGATT CAAGTCCTAT GCTCAGAGGA 
     1081 CCGAAGTCAA TGGTTTTGAC CCCCTCTTCA ATGCTATCAC TGGCTTAAAT GGTAGTGGGA 
     1141 AATCCAACAT ATTGGACTCC ATCTGCTTTT TGCTGGGCAT CTCCAACCTG TCTCAGGTTC 
     1201 GGGCTTCTAA TTTACAAGAT TTAGTTTACA AAAATGGGCA GGCTGGTATT ACCAAAGCCT 
     1261 CTGTGTCAAT CACTTTTGAT AATTCTGACA AAAAGCAAAG TCCTTTAGGA TTTGAGGTTC 
     1321 ATGATGAAAT CACAGTAACA AGGCAGGTGG TTATTGGTGG TAGAAATAAA TATTTAATCA 
     1381 ATGGAGTCAA TGCCAACAAC ACCAGAGTAC AGGATCTCTT CTGTTCTGTT GGCCTTAATG 
     1441 TTAACAACCC TCACTTTCTC ATCATGCAGG GCCGAATTAC AAAAGTATTG AATATGAAAC 
     1501 CTCCAGAGAT TTTATCCATG ATAGAAGAAG CAGCTGGAAC CAGGATGTAT GAATACAAAA 
     1561 AAATAGCTGC ACAGAAAACT ATAGAAAAAA AGGAGGCTAA GCTGAAAGAA ATTAAGACGA 
     1621 TACTTGAAGA AGAGATTACT CCAACCATTC AAAAATTAAA AGAGGAAAGA TCGTCCTACT 
     1681 TGGAGTACCA AAAAGTAATG AGAGAAATAG AACATTTGAG TCGTTTATAT ATTGCTTATC 
     1741 AGTTTTTGCT GGCTGAAGAT ACCAAAGTAC GCTCAGCTGA GGAATTAAAA GAAATGCAAG 
     1801 ATAAAGTTAT AAAGCTTCAG GAAGAATTGT CTGAGAATGA TAAAAAAATA AAAGCACTTA 
     1861 ATCATGAAAT AGAAGAATTG GAAAAAAGAA AAGATAAGGA AACTGGAGGT ATACTTCGAT 
     1921 CTTTAGAAGA TGCTCTTGCA GAGGCTCAGC GAGTTAATAC TAAATCTCAA AGCGCATTTG 
     1981 ATCTCAAGAA GAAAAATCTG GCATGTGAGG AAAGCAAACG CAAAGAGCTG GAAAAAAATA 
     2041 TGGTTGAGGA CTCAAAAACT TTAGCAGCAA AGGAAAAAGA GGTTAAAAAG ATAACAGATG 
     2101 GACTGCATGC CCTTCAAGAA GCAAGTAATA AAGATGCTGA AGCTCTGGCA GCTGCACAGC 
     2161 AGCACTTCAA TGCTGTTTCC GCTGGCCTGT CCAGTAATGA AGATGGAGCA GAAGCAACTC 
     2221 TTGCTGGTCA AATGATGGCC TGTAAAAATG ATATAAGTAA AGCTCAGACA GAAGCCAAAC 
     2281 AGGCTCAGAT GAAGTTGAAG CATGCTCAAC AGGAATTAAA GAATAAACAA GCTGAAGTTA 
     2341 AGAAGATGGA TAGTGGCTAC AGGAAGGATC AAGAAGCTCT AGAAGCTGTA AAAAGACTTA 
     2401 AAGAAAAACT TGAAGCTGAA ATGAAAAAGC TAAATTATGA AGAAAATAAA GAGGAAAGCC 
     2461 TTTTGGAAAA GCGCAGGCAG CTGTCTCGTG ATATTGGTAG ATTGAAAGAA ACATATGAAG 
     2521 CTCTATTAGC CAGATTTCCC AATCTTCGAT TTGCATACAA GGATCCAGAG AAGAACTGGA 
     2581 ATAGAAATTG TGTGAAAGGA CTTGTGGCTT CTCTGATTAG TGTGAAAGAC ACTTCTGCAA 
     2641 CCACAGCTTT AGAATTAGTG GCTGGAGAAC GACTCTACAA TGTTGTAGTA GACACAGAAG 
     2701 TTACTGGTAA AAAGCTACTA GAAAGGGGGG AACTGAAACG TCGATACACT ATAATTCCAC 
     2761 TCAATAAAAT TTCAGCCAGA TGTATTGCAC CAGAAACTCT GAGAGTTGCT CAGAATCTTG 
     2821 TTGGCCCTGA CAACGTTCAT GTGGCTCTTT CCTTGGTTGA ATATAAACCA GAACTTCAGA 
     2881 AAGCAATGGA GTTTGTCTTT GGAACAACAT TTGTTTGTGA CAATATGGAT AATGCCAAAA 
     2941 AAGTGGCCTT TGATAAGAGG ATAATGACTA GAACTGTAAC TCTCGGAGGT GATGTGTTTG 
     3001 ATCCTCATGG GACATTGAGT GGAGGTGCTC GATCCCAGGC AGCTTCCATT TTAACCAAGT 
     3061 TTCAAGAACT CAAAGATGTT CAGGATGAAC TGAGAATCAA AGAGAATGAG CTGCGGGCTC 
     3121 TAGAAGAGGA ATTAGCAGGT CTTAAAAACA CTGCTGAAAA GTATCGCCAA CTAAAACAGC 
     3181 AGTGGGAGAT GAAAACTGAA GAGGCAGATT TATTACAAAC CAAGCTCCAG CAAAGCTCAT 
     3241 ATCACAAGCA ACAAGAAGAA TTAGATGCCC TTAAAAAAAC CATTGAGGAA AGTGAGGAGA 
     3301 CTTTGAAAAA CACTAAAGAA ATCCAAAGAA AAGCAGAAGA AAAATATGAA GTATTGGAAA 
     3361 ATAAAATGAA AAATGCAGAA GCTGAAAGAG AGCGAGAACT GAAAGATGCT CAGAAAAAAC 
     3421 TGGATTGTGC CAAAACAAAG GCAGATGCAT CTAGCAAGAA GATGAAAGAA AAACAACAGG 
     3481 AAGTTGAAGC TATCACTCTG GAACTGGAAG AGCTCAAGAG AGAGCATACA TCTTACAAAC 
     3541 AACAGCTTGA AGCTGTAAAT GAAGCTATCA AATCCTATGA AAGTCAGATT GAAGTAATGG 
     3601 CAGCTGAGGT GGCTAAAAAT AAGGAGTCAG TAAATAAAGC TCAAGAAGAG GTGACCAAGC 
     3661 AAAAAGAGGT GATAACAGCC CAAGACACTG TAATTAAAGC TAAATATGCA GAAGTGGCAA 
     3721 AACACAAGGA GCAAAACAAT GATTCTCAGC TTAAAATTAA GGAATTAGAC CACAACATCA 
     3781 GCAAACATAA ACGGGAGGCT GAAGATGGTG CTGCAAAGGT ATCCAAAATG TTGAAAGATT 
     3841 ATGACTGGAT TAATGCAGAG AGACACCTCT TTGGCCAACC CAATAGTGCC TATGATTTCA 
     3901 AAACTAACAA CCCTAAAGAA GCTGGTCAGA GACTTCAGAA GTTGCAAGAA ATGAAGGAGA 
     3961 AACTAGGAAG AAATGTCAAT ATGAGAGCTA TGAATGTATT GACAGAAGCT GAAGAGCGAT 
     4021 ACAATGACTT GATGAAGAAG AAGAGAATTG TAGAAAATGA CAAATCCAAA ATTCTTACAA 
     4081 CTATAGAAGA CCTTGACCAG AAGAAAAACC AAGCCCTAAA TATTGCATGG CAAAAGGTGA 
     4141 ACAAGGACTT TGGGTCTATT TTTTCTACTC TTTTGCCTGG TGCTAATGCT ATGCTTGCAC 
     4201 CACCAGAGGG TCAAACTGTT TTGGATGGTC TGGAGTTCAA GGTTGCCTTG GGAAATACCT 
     4261 GGAAAGAAAA CCTAACTGAA CTTAGTGGTG GTCAGAGGTC TTTAGTGGCC TTGTCATTAA 
     4321 TACTGTCCAT GCTTCTCTTC AAACCTGCTC CAATTTATAT CCTTGATGAG GTAGATGCAG 
     4381 CCTTGGATCT TTCTCATACC CAAAACATTG GACAGATGCT GCGTACTCAT TTCACACATT 
     4441 CTCAGTTCAT TGTGGTGTCA CTAAAAGAAG GTATGTTCAA CAATGCAAAC GTTCTTTTCA 
     4501 AAACCAAGTT TGTGGATGGT GTTTCTACAG TAGCCAGATT TACTCAATGT CAAAATGGAA 
     4561 AGATTTCAAA GGAAGCAAAA TCCAAGGCAA AACCACCCAA AGGAGCACAT GTGGAAGTTA 
     4621 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4681 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4741 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4801 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4861 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4921 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4981 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5041 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5101 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5161 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5221 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5281 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5341 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5401 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5461 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5521 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5581 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5641 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5701 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5761 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5821 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5881 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5941 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6001 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6061 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6121 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6181 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6241 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6301 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6361 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6421 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6481 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6541 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6601 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6661 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6721 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6781 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6841 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6901 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6961 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7021 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7081 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7141 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7201 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7261 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7321 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7381 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7441 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7501 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7561 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7621 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7681 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7741 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7801 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7861 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7921 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7981 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8041 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8101 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8161 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8221 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8281 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8341 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8401 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8461 AACGCGAATT TT
//