LOCUS       RC217642                8649 bp    DNA     circular  SYN 
DEFINITION  SYTL2 (Myc-DDK-tagged)-Human synaptotagmin-like 2 (SYTL2), transcript variant d
ACCESSION   NM_206928
VERSION     NM_206928.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4806..4897 
                     /label="Myc-DDK"  
     misc_feature    4927..5512 
                     /label="PolyA signal"  
     misc_feature    4984..5003 
                     /label="Restriction Enzyme"  
     misc_feature    5788..6460 
                     /label="ColE1"  
     misc_feature    6890..7684 
                     /label="Kan/Neo"  
     misc_feature    7719..8021 
                     /label="SV40 ori"  
     misc_feature    8107..8584 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4788..4794 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4788 
                     /label="SYTL2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGAACCC AATGCAGAAC AGGTCTATAA TCCCTCTCAG TTTGAGAATT 
     1081 TGAGAAAGTT TTGGGACTTA GAAGCTAATT CAAACAGTAA GGATAATGAC AAGAATATTA 
     1141 CCACCACAAG CCAAAAAAAT TCTGCACCTT TTAATAGGCA GAAACACAAG GAATTCAGCG 
     1201 ACATTAAATT ATCAGGTAAA AATACCCATG AAGCAGAGGT GCTTCTAAGC CCAAAAAAAG 
     1261 TTATGGCAAG AGAGGAAATG GAGAAATTAA ATTCAAAGGG CATACTCCAG GTGCTACCAG 
     1321 ATGAAATCAC ATTTCCTTTG AGTCCACTTA GAAAGTATAC TTATCAGTTG CCAGGAAATG 
     1381 AGTCATCAAA GGAAAATGTG GAAAAGAATA CGGAAGGGAT TGTTACTCCA GTGTTTAAGG 
     1441 AAGAAAAGGA TTACTCAGAA CAAGAGATTC AAGAATCCAT AATAAAAACC AATGTTTTGT 
     1501 CTAAAGACTG CAAAGACACT TTTAATGACA GCTTGCAGAA ACTGCTTTCA GAAACCTCAA 
     1561 CACCAGCAAT TCAACCCTCT GGTGGAAAAG TTCATGGAAA ACAAGTGCTT GAACCAAGTG 
     1621 TTTCTGAAAA TAGGACATGG CCTCAAAAAA CAGATTTTGC TGATACTGAG GAAGAAGTCA 
     1681 AAGGACCTGA GAAGATCATT AATGAGCATG TTGACAAAAC AGTAGTTCAT CCAAAGGTTA 
     1741 AACGGAACTC TTTGACTGCT AGTCTAGACA AACTCCTGAA GGAAGCAACT GGAACTTCAC 
     1801 CCTCTCCCTT GCAAGCCAAG TTGGCGCCTG TTATCACTGG AACCAACTCT AAGCTGGAAG 
     1861 AGGGGAGATT TTTTGGAAAA GGGATAGAAC AGAGTCACAA TACTTCAGCT GATAAGAGAG 
     1921 AAATACTAGC TCCTTTTCCA GTGAGAGATG AAACTTTTGG AAATACAGCT CTCCTCAAGA 
     1981 AAGCTGAAAG TGGTGAGTGC CAGCTAAGCA CACAGAATTT GATTCAGATG GCTGCAGAAG 
     2041 ATTCTCATCC ATTGGATCCA ACTTCCCAGC TTTCCAGAAA GGGTTCTTTT GGGGATGTGG 
     2101 CCAGCCCTCC CCAAGATATG CTTTTTCCCC AGGATGCTCA TCTTGTTCCC CAGGCTAGGG 
     2161 TACACCCTTC TCAAACGGAA ATTTCGGAGA CTGTAGAGAA AGTCATTCTT CCACCCAGAC 
     2221 CTGTATTGAA TGATGTAAGT GCTGCATTAC AGAAGCTGTG TGGAGAAGTA TGGTTAAGTT 
     2281 ATCCAGCTGG AAGGGAAGTA GGTCCTGGAG AAGTGAACCC AGAATTTCCT GAAGCAGTAC 
     2341 AGCCAGTATG TAGCCCCCTA AATCCTCCAG GAGTGATATC ACCATGGGCT ACGATGGACA 
     2401 CCATAGTTCC AGACAGGAAG GATTTTTATT CCTCCAATGT AGTTCCTGAT AAAACTCATG 
     2461 AAGTTGGATC TTATTTAGCT GCCCAAATGT CTCCATCAGA CCAGACGCTT AGCTCATTTG 
     2521 CTTCCATTGT TGCTCAATAT GGCAAAGGCC TCCCTCAGGA AGTGGAAGAA ATTGTGAGGG 
     2581 AAACAATTGT TCAACCCAAA TCAGAGTTCC TCGAATTCAG TGCTGGCTTA GAAAAACTAC 
     2641 TGAAGGAAGA AACTGAAACC TTCCCCTCAA AATATGAAAG TGATACAGGG AATCTTTCTC 
     2701 CATCAAAGTT AATAGGTAGT ACAGAGGAGC CCAGGCGAGC CACTTCTGAA TGCCATCCTG 
     2761 AGGAATTAAA AGAAACAGTA GAAAAGGCCG AGGCTCCGTT AATAACTGAG AGTGCTTTTG 
     2821 ATGCTGGTTT TGAGAAACTT CTTAAAGAAA TAACTGAAGC TCCTCCTTAT CAGCCCCAGG 
     2881 TGTCAGTGAG AGAAGAAACT CACGAGAAGG AGTCCTCACA GTCAGAGCAG ACCAGGTTCT 
     2941 TGGGGACAGT GCCCCATTTT TACAGGGCAG CCTCACAGAC CTCTGAAATG AAGGATAAAA 
     3001 GTAATGGTTT GGAATCTCAA GTCAACCAAT GTGATAAAAT GTTGGGAGGA GACGCACTTG 
     3061 TGACTGATTT ATTGGTAGAT TTTTGTGGTT CCAGAAGTGG AGTTGAGATC CCTAGAACCC 
     3121 CACAACTTTA TGTGGCTCAT GAAATAGGGA CCATTAAAAC TGTAACCCCC CCAGAGGACA 
     3181 GGGACAGTGA AAGTGGGGTT GCAGGGGGAC AAGGGACTCT TCAGGAACCT GGCTTTGGAG 
     3241 AGGCTTCTGA AGCAATTAGT GTGTCCAGAA ATAGGCAACC CATTCCTCTC CTGATGAACA 
     3301 AAGAAAACTC TACAAAAACA AGTAAAGTTG AATTGACTCT AGCATCGCCA TATATGAAAC 
     3361 AAGAGAAAGA GGAAGAAAAA GAAGGTTTCT CTGAGTCTGA TTTTTCAGAT GGAAACACCA 
     3421 GTTCTAATGC AGAGAGCTGG AGAAATCCTT CCAGTTCAGA AGAAGAACCC AGTCCTGTTT 
     3481 TGAAAACTTT GGAAAGGAGT GCCGCTAGGA AAATGCCTTC CAAAAGTCTA GAAGACATTT 
     3541 CATCAGATTC ATCAAATCAA GCAAAAGTAG ATAATCAGCC AGAAGAATTA GTGCGTAGTG 
     3601 CTGAAGATGT TTCCACAGTG CCTACACAAC CTGATAATCC ATTTTCTCAC CCTGACAAAC 
     3661 TCAAAAGGAT GAGCAAGTCT GTTCCAGCAT TTCTCCAAGA TGAGAGTGAT GACAGAGAAA 
     3721 CAGATACAGC ATCAGAAAGC AGTTACCAGC TCAGCAGACA CAAGAAGAGC CCGAGCTCTT 
     3781 TAACCAATCT TAGCAGCTCC TCTGGCATGA CGTCCTTGTC TTCTGTGAGT GGCAGTGTGA 
     3841 TGAGTGTTTA TAGTGGAGAC TTTGGCAATC TGGAAGTTAA AGGAAATATT CAGTTTGCAA 
     3901 TTGAATATGT GGAGTCACTG AAGGAGTTGC ATGTTTTTGT GGCCCAGTGT AAGGACTTAG 
     3961 CAGCAGCGGA TGTAAAAAAA CAGCGTTCAG ACCCATATGT AAAGGCCTAT TTGCTACCAG 
     4021 ACAAAGGCAA AATGGGCAAG AAGAAAACAC TCGTAGTGAA GAAAACCTTG AATCCTGTGT 
     4081 ATAACGAAAT ACTGCGGTAT AAAATTGAAA AACAAATCTT AAAGACACAG AAATTGAACC 
     4141 TGTCCATTTG GCATCGGGAT ACATTTAAGC GCAATAGTTT CCTAGGGGAG GTGGAACTTG 
     4201 ATTTGGAAAC ATGGGACTGG GATAACAAAC AGAATAAACA ATTGAGATGG TACCCTCTGA 
     4261 AGCGGAAGAC AGCACCAGTT GCCCTTGAAG CAGAAAACAG AGGTGAAATG AAACTAGCTC 
     4321 TCCAGTATGT CCCAGAGCCA GTCCCTGGTA AAAAGCTTCC TACAACTGGA GAAGTGCACA 
     4381 TCTGGGTGAA GGAATGCCTT GATCTACCAC TGCTAAGGGG AAGTCATCTA AATTCTTTTG 
     4441 TTAAATGTAC CATCCTTCCA GATACAAGTA GGAAAAGTCG CCAGAAGACA AGAGCTGTAG 
     4501 GGAAAACCAC CAACCCTATC TTCAACCACA CTATGGTGTA TGATGGGTTC AGGCCTGAAG 
     4561 ATCTGATGGA AGCCTGTGTA GAGCTTACTG TCTGGGACCA TTACAAATTA ACCAACCAAT 
     4621 TTTTGGGAGG TCTTCGTATT GGCTTTGGAA CAGGTAAAAG TTATGGGACT GAAGTGGACT 
     4681 GGATGGACTC TACTTCAGAG GAAGTTGCTC TCTGGGAGAA GATGGTAAAC TCCCCCAATA 
     4741 CTTGGATTGA AGCAACACTG CCTCTCAGAA TGCTTTTGAT TGCCAAGATT TCCAAAACGC 
     4801 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4861 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4921 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4981 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5041 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5101 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5161 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5221 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5281 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5341 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5401 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5461 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5521 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5581 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5641 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5701 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5761 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5821 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5881 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5941 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6001 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6061 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6121 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6181 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6241 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6301 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6361 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6421 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6481 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6541 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6601 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6661 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6721 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6781 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6841 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6901 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6961 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7021 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7081 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7141 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7201 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7261 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7321 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7381 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7441 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7501 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7561 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7621 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7681 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7741 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7801 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7861 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7921 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8101 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8161 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8221 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8281 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8341 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8401 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8461 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8521 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8581 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8641 GCGAATTTT
//