LOCUS       RC217591                9066 bp    DNA     circular  SYN 
DEFINITION  CCNB3 (Myc-DDK-tagged)-Human cyclin B3 (CCNB3), transcript variant 3
ACCESSION   NM_033031
VERSION     NM_033031.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5223..5314 
                     /label="Myc-DDK"  
     misc_feature    5344..5929 
                     /label="PolyA signal"  
     misc_feature    5401..5420 
                     /label="Restriction Enzyme"  
     misc_feature    6205..6877 
                     /label="ColE1"  
     misc_feature    7307..8101 
                     /label="Kan/Neo"  
     misc_feature    8136..8438 
                     /label="SV40 ori"  
     misc_feature    8524..9001 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5205..5211 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5205 
                     /label="CCNB3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTACTGCCA CTACCACCCC AGAGCTCCAA ACCTGTGCCT AAGAAATCTC 
     1081 AGTCCAGCAA AATTGTGCCC AGTCATCATG ACCCATCTGA AAAGACGGGG GAGAATTGCC 
     1141 AAACGAAGAT ATCTCCATCT TCACTTCAGG AGTCTCCATC TTCACTTCAG GGAGCACTCA 
     1201 AAAAGAGATC AGCTTTTGAA GATCTCACTA ATGCTTCTCA ATGTCAACCT GTCCAGCCCA 
     1261 AGAAAGAAGC CAATAAAGAG TTTGTAAAAG TTGTTTCCAA GAAGATAAAC AGGAACACAC 
     1321 ATGCTCTTGG ACTGGCCAAA AAGAATAAGC GGAATCTAAA ATGGCATAAG CTGGAAGTCA 
     1381 CACCAGTAGT AGCCTCTACT ACCGTGGTAC CAAACATTAT GGAGAAACCA CTCATTCTAG 
     1441 ACATATCCAC CACCTCCAAA ACACCCAACA CTGAGGAGGC ATCTCTCTTC AGAAAGCCAT 
     1501 TAGTTTTAAA GGAGGAACCC ACTATTGAGG ATGAAACCCT TATCAATAAG TCATTATCTT 
     1561 TAAAAAAGTG CTCAAATCAT GAGGAGGTGT CCTTACTGGA AAAGCTACAG CCCCTGCAGG 
     1621 AGGAGAGTGA CAGTGATGAT GCGTTTGTTA TAGAGCCAAT GACTTTTAAG AAGACACATA 
     1681 AAACTGAGGA GGCAGCCATC ACCAAGAAGA CATTATCCTT AAAGAAGAAG ATGTGTGCAA 
     1741 GTCAGCGGAA GCAGTCCTGC CAGGAAGAGT CGTTGGCTGT GCAGGATGTC AATATGGAAG 
     1801 AGGATTCCTT CTTTATGGAG TCAATGAGTT TTAAGAAGAA GCCTAAAACT GAGGAGTCAA 
     1861 TCCCCACCCA TAAGTTATCA TCTTTAAAGA AGAAATGTAC CATTTATGGG AGGATATGCC 
     1921 ACTTTAGGAA GCCACCAGTA TTGCAGACAA CCATCTGTGG AGCAATGTCC TCCATTAAGA 
     1981 AGCCTACCAC TGAGAAGGAG ACACTTTTCC AAGAGCTATC TGTATTGCAA GAGAAACACA 
     2041 CCACTGAGCA TGAGATGTCC ATCTTGAAGA AATCATTGGC CTTGCAGAAG ACCAACTTTA 
     2101 AAGAGGATTC CCTTGTTAAG GAGTCGTTAG CCTTTAAGAA GAAGCCTAGC ACTGAGGAGG 
     2161 CAATCATGAT GCCAGTAATA TTGAAGGAGC AGTGCATGAC TGAGGGGAAG AGGCCCCGTC 
     2221 TGAAGCCATT AGTATTGCAG GAGATCACCT CTGGAGAGAA GTCGCTCATT ATGAAGCCAT 
     2281 TGTCCATTAA AGAAAAGCCA TCTACTGAGA AGGAGTCCTT TTCCCAGGAA CCATCTGCAT 
     2341 TGCAAAAGAA GCACACCACT CAGGAGGAGG TTTCCATCTT AAAGGAGCCC TCGTCCTTGC 
     2401 TAAAGTCTCC AACTGAGGAG TCACCTTTTG ATGAGGCTTT GGCTTTTACA AAGAAGTGTA 
     2461 CCATTGAGGA GGCACCCCCC ACCAAGAAGC CTTTAATTTT AAAGAAGAAG CATGCCACTC 
     2521 AGGGGACAAT GTCCCACTTG AAGAAACCAC TAATATTACA GACCACCTCT GGAGAAAAGT 
     2581 CACTTATTAA GGAGCCACTG CCCTTTAAAG AAGAAAAAGT GTCTTTAAAG AAAAAGTGTA 
     2641 CCACACAAGA GATGATGTCC ATCTGTCCAG AACTGTTGGA CTTTCAGGAT ATGATTGGTG 
     2701 AAGATAAGAA TTCCTTCTTT ATGGAGCCAA TGTCATTTAG GAAGAGCCCT ACAACTGAGG 
     2761 AGACAGTACT TACCAAGACA TCGTTGTCTT TACAGGAAAA GAAAATTACT CAGGGGGAGA 
     2821 TGTCCCACTT AAAGAAGCCA CTGGTCTTGC AGAAGATCAC TTCTGAGGAG GAGTCATTCT 
     2881 ATAAGAAGCT GTTGCCCTTT AAGATGAAAT CTACAACGGA AGAAAAGTTC CTCTCCCAGG 
     2941 AACCATCTGC ATTGAAAGAG AAGCATACCA CCTTGCAGGA AGTGTCCCTC TCAAAAGAGT 
     3001 CATTGGCCAT CCAAGAGAAG GCTACCACTG AGGAGGAATT CTCTCAGGAA CTATTTTCAT 
     3061 TGCATGTTAA GCATACCAAC AAAAGTGGGT CCCTCTTCCA GGAGGCTTTG GTCTTGCAAG 
     3121 AGAAGACTGA TGCCGAAGAG GATTCCTTGA AGAACTTGTT GGCTTTGCAG GAGAAAAGCA 
     3181 CCATGGAAGA AGAGTCCCTT ATCAATAAGC TATTGGCTCT GAAGGAGGAG CTTTCTGCTG 
     3241 AGGCAGCCAC AAACATACAG ACACAATTAT CTTTAAAGAA GAAGTCCACT TCTCATGGAA 
     3301 AAGTGTTCTT CCTGAAGAAG CAGTTGGCTT TGAATGAGAC CATCAATGAA GAGGAGTTCC 
     3361 TTAATAAGCA GCCACTGGCC TTGGAGGGGT ATCCCAGCAT TGCGGAGGGG GAGACCCTCT 
     3421 TCAAGAAGCT TTTGGCCATG CAGGAGGAGC CCAGCATTGA GAAGGAAGCT GTCCTCAAGG 
     3481 AGCCCACTAT TGACACAGAA GCTCACTTTA AGGAACCTTT GGCCTTGCAG GAGGAGCCCA 
     3541 GCACTGAGAA GGAGGCTGTC CTCAAGGAGC CCAGTGTTGA CACAGAAGCT CACTTTAAGG 
     3601 AAACTTTGGC CTTGCAGGAG AAGCCCAGCA TTGAGCAGGA GGCCCTCTTT AAGCGACACT 
     3661 CAGCTTTGTG GGAGAAGCCC AGCACTGAGA AGGAGACCAT CTTCAAGGAG TCTTTGGACT 
     3721 TGCAAGAGAA GCCCAGCATT AAGAAAGAGA CCCTCCTCAA AAAGCCATTA GCCTTGAAGA 
     3781 TGTCTACCAT CAATGAGGCA GTCCTCTTCG AAGATATGAT AGCTCTGAAT GAGAAACCCA 
     3841 CCACTGGGAA GGAGTTGTCC TTCAAGGAGC CATTAGCCTT ACAAGAGAGT CCCACCTACA 
     3901 AGGAAGACAC CTTTCTCAAA ACATTGTTGG TCCCCCAAGT TGGAACCAGC CCAAATGTGT 
     3961 CTAGCACTGC CCCTGAATCC ATAACCAGCA AGTCCAGCAT TGCTACCATG ACCAGTGTGG 
     4021 GCAAATCTGG TACCATCAAT GAGGCATTCC TCTTCGAAGA TATGATAACT CTGAATGAGA 
     4081 AACCCACCAC TGGGAAGGAG TTGTCCTTCA AGGAGCCATT GGCCTTACAA GAGAGTCCCA 
     4141 CCTGCAAGGA AGACACCTTT CTGGAAACAT TCTTGATCCC CCAAATTGGA ACCAGCCCAT 
     4201 ATGTGTTTAG CACCACCCCT GAATCCATAA CAGAGAAGTC CAGCATTGCA ACCATGACCA 
     4261 GCGTGGGCAA GTCCAGGACC ACCACCGAGT CCAGTGCATG TGAATCTGCT TCTGATAAAC 
     4321 CTGTCTCACC ACAGGCCAAG GGAACACCAA AGGAGATAAC CCCACGGGAA GATATTGATG 
     4381 AGGACAGCAG TGATCCAAGT TTCAACCCAA TGTATGCCAA GGAAATCTTC AGTTACATGA 
     4441 AAGAGAGAGA GGAACAGTTT ATACTTACAG ATTACATGAA CAGGCAGATT GAAATCACCA 
     4501 GTGACATGAG GGCCATTCTT GTGGACTGGT TGGTGGAGGT GCAGGTGTCC TTTGAGATGA 
     4561 CCCATGAGAC CCTGTACTTG GCAGTGAAGC TGGTGGATCT CTACCTAATG AAGGCAGTAT 
     4621 GCAAGAAGGA TAAGTTACAA CTCCTTGGTG CCACTGCCTT TATGATTGCA GCAAAATTTG 
     4681 AGGAGCACAA CTCACCTCGT GTGGATGACT TTGTGTACAT CTGTGATGAT AATTATCAGC 
     4741 GATCTGAGGT ACTCAGCATG GAAATCAACA TCCTGAACGT CCTCAAATGT GACATTAACA 
     4801 TTCCCATCGC CTACCATTTT CTGCGCAGAT ATGCTAGGTG TATCCACACC AACATGAAGA 
     4861 CACTGACCTT GTCCCGCTAC ATCTGCGAGA TGACCCTGCA GGAATACCAC TATGTCCAGG 
     4921 AGAAGGCTTC CAAGCTAGCT GCTGCCTCCT TACTCCTGGC CCTCTACATG AAGAAGCTCG 
     4981 GATACTGGGT TCCCTTCCTG GAGCATTACA GTGGCTACAG TATCTCTGAG CTTCACCCCT 
     5041 TGGTCAGACA GCTGAACAAA CTGCTGACTT TCAGTTCTTA CGATAGTCTC AAGGCTGTGT 
     5101 ATTACAAGTA TTCTCACCCG GTCTTCTTTG AAGTCGCCAA AATCCCTGCC TTGGATATGT 
     5161 TGAAGCTGGA GGAGATTTTG AACTGTGATT GTGAGGCTCA GGGCCTGGTA CTCACGCGTA 
     5221 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     5281 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     5341 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     5401 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     5461 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     5521 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5581 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5641 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5701 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5761 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5821 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5881 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5941 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     6001 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     6061 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     6121 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     6181 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     6241 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     6301 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     6361 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     6421 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     6481 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6541 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6601 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6661 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6721 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6781 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6841 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6901 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6961 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     7021 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     7081 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     7141 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     7201 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     7261 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     7321 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     7381 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     7441 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     7501 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7561 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7621 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7681 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7741 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7801 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7861 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7921 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7981 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     8041 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     8101 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     8161 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     8221 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     8281 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     8341 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     8401 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     8461 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     8521 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8581 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8641 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8701 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8761 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8821 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8881 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8941 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     9001 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     9061 AATTTT
//