LOCUS       RC217570                9210 bp    DNA     circular  SYN 
DEFINITION  PTPRK (Myc-DDK-tagged)-Human protein tyrosine phosphatase, receptor type, K (PTPRK), transcript variant 2
ACCESSION   NM_002844
VERSION     NM_002844.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5365..5456 
                     /label="Myc-DDK"  
     misc_feature    5486..6071 
                     /label="PolyA signal"  
     misc_feature    5543..5562 
                     /label="Restriction Enzyme"  
     misc_feature    6347..7019 
                     /label="ColE1"  
     misc_feature    7449..8243 
                     /label="Kan/Neo"  
     misc_feature    8278..8580 
                     /label="SV40 ori"  
     misc_feature    8666..9143 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5340..5347 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5340 
                     /label="PTPRK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATACGACT GCGGCGGCGG CGCTGCCTGC TTTTGTGGCG CTCTTGCTCC 
     1081 TCTCTCCTTG GCCTCTCCTG GGATCGGCCC AAGGCCAGTT CTCCGCAGGT GGCTGTACTT 
     1141 TTGATGATGG TCCAGGGGCC TGTGATTACC ACCAGGATCT GTATGATGAC TTTGAATGGG 
     1201 TGCATGTTAG TGCTCAAGAG CCTCATTATC TACCACCCGA GATGCCCCAA GGTTCCTATA 
     1261 TGATAGTGGA CTCTTCAGAT CACGACCCTG GAGAAAAAGC CAGACTTCAG CTGCCTACAA 
     1321 TGAAGGAGAA CGACACTCAC TGCATTGATT TCAGTTACCT ATTATATAGC CAGAAAGGAC 
     1381 TGAATCCTGG CACTTTGAAC ATATTAGTTA GGGTGAATAA AGGACCTCTT GCCAATCCAA 
     1441 TTTGGAATGT GACTGGATTC ACGGGTAGAG ATTGGCTTCG GGCTGAGCTA GCAGTGAGCA 
     1501 CCTTTTGGCC CAATGAATAT CAGGTAATAT TTGAAGCTGA AGTCTCAGGA GGGAGAAGTG 
     1561 GTTATATTGC CATTGATGAC ATCCAAGTAC TGAGTTATCC TTGTGATAAA TCTCCTCATT 
     1621 TCCTCCGTCT AGGGGATGTA GAGGTGAATG CAGGGCAAAA CGCTACATTT CAGTGCATTG 
     1681 CCACAGGGAG AGATGCTGTG CATAACAAGT TATGGCTCCA GAGACGAAAT GGAGAAGATA 
     1741 TACCAGTAGC CCAGACTAAG AACATCAATC ATAGAAGGTT TGCCGCTTCC TTCAGATTGC 
     1801 AAGAAGTGAC AAAAACTGAC CAGGATTTGT ATCGCTGTGT AACTCAGTCA GAACGAGGTT 
     1861 CCGGTGTGTC CAATTTTGCT CAACTTATTG TGAGAGAACC GCCAAGACCC ATTGCTCCTC 
     1921 CTCAGCTTCT TGGTGTTGGG CCTACATATT TGCTGATCCA ACTAAATGCC AACTCGATCA 
     1981 TTGGCGATGG TCCTATCATC CTGAAAGAAG TAGAGTACCG AATGACATCA GGATCCTGGA 
     2041 CAGAAACCCA TGCAGTCAAT GCTCCAACTT ACAAATTATG GCATTTAGAT CCAGATACCG 
     2101 AATATGAGAT CCGAGTTCTA CTTACAAGAC CTGGTGAAGG TGGAACGGGG CTCCCAGGAC 
     2161 CTCCACTAAT CACCAGAACA AAATGTGCAG AACCTATGAG AACCCCAAAG ACATTAAAGA 
     2221 TTGCTGAAAT ACAGGCAAGA CGGATTGCTG TGGACTGGGA ATCCTTGGGT TACAACATTA 
     2281 CGCGTTGCCA CACTTTTAAT GTCACTATCT GCTACCATTA CTTCCGTGGT CACAACGAGA 
     2341 GCAAGGCAGA CTGTTTGGAC ATGGACCCCA AAGCCCCTCA GCATGTTGTG AACCATCTGC 
     2401 CACCTTATAC AAATGTCAGC CTCAAGATGA TCCTAACCAA TCCAGAGGGA AGGAAGGAGA 
     2461 GTGAAGAGAC AATTATTCAA ACTGATGAAG ATGTGCCTGG TCCCGTACCA GTAAAATCTC 
     2521 TTCAAGGAAC ATCCTTTGAA AATAAGATCT TCTTGAACTG GAAAGAACCT TTGGATCCAA 
     2581 ATGGAATCAT CACTCAATAT GAGATCAGCT ATAGCAGTAT AAGATCATTT GATCCTGCAG 
     2641 TTCCAGTGGC TGGACCTCCC CAGACTGTAT CAAATTTATG GAACAGTACA CACCATGTCT 
     2701 TTATGCATCT CCACCCTGGA ACCACGTACC AGTTTTTCAT AAGAGCCAGC ACGGTCAAAG 
     2761 GCTTTGGTCC AGCCACAGCC ATCAATGTCA CCACCAATAT CTCAGCTCCA ACTTTACCTG 
     2821 ACTATGAAGG AGTTGATGCC TCTCTCAATG AAACTGCCAC CACAATAACT GTATTGTTGA 
     2881 GACCAGCACA AGCCAAAGGT GCTCCTATCA GTGCTTATCA GATTGTTGTG GAAGAACTGC 
     2941 ACCCACACCG AACCAAGAGA GAAGCCGGAG CCATGGAATG CTACCAGGTT CCTGTCACAT 
     3001 ACCAAAATGC CATGAGTGGG GGTGCACCGT ATTACTTTGC TGCAGAACTC CCCCCGGGAA 
     3061 ACCTACCTGA GCCTGCCCCG TTCACTGTGG GTGACAATCG GACCTACCAA GGCTTTTGGA 
     3121 ACCCTCCTTT GGCTCCGCGC AAAGGATACA ACATCTATTT CCAGGCGATG AGCAGTGTGG 
     3181 AGAAGGAAAC TAAAACCCAG TGCGTACGCA TTGCTACAAA AGCAGCAGCA ACAGAAGAAC 
     3241 CAGAAGTGAT CCCAGATCCC GCCAAGCAGA CAGACAGAGT GGTGAAAATA GCAGGAATTA 
     3301 GTGCTGGAAT TTTGGTGTTC ATCCTCCTTC TCCTAGTTGT CATATTAATT GTAAAAAAGA 
     3361 GCAAACTTGC TAAAAAACGC AAAGATGCCA TGGGGAATAC CCGGCAGGAG ATGACTCACA 
     3421 TGGTGAATGC AATGGATCGA AGTTATGCTG ATCAGAGCAC TCTGCATGCA GAAGATCCTC 
     3481 TTTCCATCAC CTTCATGGAC CAACATAACT TTAGTCCAAG ATATGAGAAC CACAGTGCTA 
     3541 CAGCAGAGTC CAGTCGCCTT CTAGACGTAC CTCGCTACCT CTGTGAGGGG ACGGAATCCC 
     3601 CTTACCAGAC AGGACAGCTG CATCCAGCCA TCAGGGTAGC TGATTTACTG CAGCACATTA 
     3661 ATCTCATGAA GACATCAGAC AGCTATGGGT TCAAAGAGGA ATATGAGAGC TTTTTTGAAG 
     3721 GACAGTCAGC ATCTTGGGAT GTAGCTAAAA AAGATCAAAA TAGAGCAAAA AACCGATATG 
     3781 GAAACATTAT AGCATATGAT CACTCCAGAG TGATTTTGCA ACCCGTAGAG GATGATCCTT 
     3841 CCTCAGATTA TATTAATGCC AACTATATTG ATGGCTACCA GAGACCAAGT CATTACATTG 
     3901 CAACCCAAGG TCCCGTTCAT GAAACAGTGT ATGATTTCTG GAGGATGATT TGGCAAGAAC 
     3961 AATCTGCTTG CATTGTGATG GTTACAAATT TAGTTGAGGT TGGCCGGGTT AAATGCTATA 
     4021 AATATTGGCC TGATGATACT GAAGTTTATG GTGACTTCAA AGTAACGTGT GTAGAAATGG 
     4081 AACCACTTGC TGAATATGTA GTTAGGACAT TCACCCTGGA AAGGAGGGGG TACAATGAAA 
     4141 TCCGTGAAGT TAAACAGTTC CATTTCACGG GCTGGCCTGA CCATGGAGTG CCCTACCATG 
     4201 CTACAGGGCT GCTTTCCTTT ATCCGGCGAG TCAAGTTATC AAACCCTCCC AGTGCTGGCC 
     4261 CCATCGTTGT ACATTGCAGT GCTGGTGCTG GACGAACTGG CTGCTACATT GTGATTGACA 
     4321 TCATGCTAGA CATGGCTGAA AGAGAGGGTG TTGTTGATAT TTACAATTGT GTCAAAGCCT 
     4381 TAAGATCTCG GCGTATTAAT ATGGTCCAGA CAGAGGAACA GTACATTTTT ATTCATGATG 
     4441 CCATTTTAGA AGCCTGCTTA TGTGGAGAAA CTGCCATACC TGTCTGTGAA TTTAAAGCTG 
     4501 CATATTTTGA TATGATTAGA ATAGACTCCC AGACTAACTC TTCACATCTC AAGGATGAAT 
     4561 TTCAGACTCT GAATTCAGTC ACCCCTCGAC TACAAGCTGA AGACTGCAGT ATAGCGTGCC 
     4621 TGCCAAGGAA CCATGACAAG AACCGTTTCA TGGACATGCT GCCACCTGAC AGATGTCTGC 
     4681 CTTTTTTAAT TACAATTGAT GGGGAGAGCA GTAACTACAT CAATGCTGCT CTTATGGACA 
     4741 GCTACAGGCA ACCAGCTGCT TTCATCGTCA CACAATACCC TCTGCCAAAC ACTGTAAAAG 
     4801 ACTTCTGGAG ATTAGTGTAT GATTATGGCT GTACCTCCAT TGTGATGTTA AACGAAGTCG 
     4861 ACTTGTCCCA GGGCTGCCCT CAGTACTGGC CAGAGGAAGG GATGCTACGA TATGGCCCCA 
     4921 TCCAAGTGGA ATGTATGTCT TGTTCAATGG ACTGTGATGT GATCAACCGG ATTTTTAGGA 
     4981 TATGCAATCT AACAAGACCA CAGGAAGGTT ATCTGATGGT GCAACAGTTT CAGTACCTAG 
     5041 GATGGGCTTC TCATCGAGAA GTGCCTGGAT CCAAAAGGTC ATTCTTGAAA CTGATACTTC 
     5101 AGGTGGAAAA GTGGCAGGAG GAATGCGAGG AAGGGGAAGG CCGGACGATT ATCCACTGCC 
     5161 TAAATGGTGG CGGGCGAAGT GGCATGTTCT GTGCTATAGG CATCGTTGTT GAAATGGTGA 
     5221 AACGGCAAAA TGTTGTCGAT GTTTTCCATG CAGTAAAGAC ACTGAGGAAC AGCAAGCCAA 
     5281 ACATGGTGGA AGCCCCGGAG CAATACCGTT TCTGCTATGA TGTAGCTTTG GAGTACCTGG 
     5341 AATCATCTAG CGGACCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5401 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5461 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5521 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5581 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5641 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5701 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5761 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     5821 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     5881 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     5941 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6001 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6061 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6121 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6181 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6241 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6301 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6361 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6421 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6481 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6541 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6601 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6661 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6721 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6781 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     6841 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     6901 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     6961 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7021 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7081 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7141 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7201 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7261 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7321 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7381 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7441 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7501 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7561 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7621 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7681 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7741 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7801 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     7861 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     7921 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     7981 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8041 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8101 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8161 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8221 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8281 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8341 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8401 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8461 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8521 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8581 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8641 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8701 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8761 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     8821 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     8881 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     8941 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9001 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9061 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9121 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9181 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//