LOCUS       RC217559                9417 bp    DNA     circular  SYN 
DEFINITION  EPRS (Myc-DDK-tagged)-Human glutamyl-prolyl-tRNA synthetase (EPRS)
ACCESSION   NM_004446
VERSION     NM_004446.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5574..5665 
                     /label="Myc-DDK"  
     misc_feature    5695..6280 
                     /label="PolyA signal"  
     misc_feature    5752..5771 
                     /label="Restriction Enzyme"  
     misc_feature    6556..7228 
                     /label="ColE1"  
     misc_feature    7658..8452 
                     /label="Kan/Neo"  
     misc_feature    8487..8789 
                     /label="SV40 ori"  
     misc_feature    8875..9352 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5556..5562 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5556 
                     /label="EPRS"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACGCTC TCTCTGACCG TGAATTCAGG AGACCCTCCG CTAGGAGCTT 
     1081 TGCTGGCAGT AGAACACGTG AAAGACGATG TCAGCATTTC CGTTGAAGAA GGGAAAGAGA 
     1141 ATATTCTTCA TGTTTCTGAA AATGTGATAT TCACAGATGT GAATTCTATA CTTCGCTACT 
     1201 TGGCTAGAGT TGCAACTACA GCTGGGTTAT ATGGCTCTAA TCTGATGGAA CATACTGAGA 
     1261 TTGATCACTG GTTGGAGTTC AGTGCTACAA AATTATCTTC ATGTGATTCC TTTACTTCTA 
     1321 CAATTAATGA ACTCAATCAT TGCCTGTCTC TGAGAACATA CTTAGTTGGA AACTCCTTGA 
     1381 GTTTAGCAGA TTTATGTGTT TGGGCCACCC TAAAAGGAAA TGCTGCCTGG CAAGAACAGT 
     1441 TGAAACAGAA GAAAGCTCCA GTTCATGTAA AACGTTGGTT TGGCTTTCTT GAAGCCCAGC 
     1501 AGGCCTTCCA GTCAGTAGGT ACCAAGTGGG ATGTTTCAAC AACCAAAGCT CGAGTGGCAC 
     1561 CTGAGAAAAA GCAAGATGTT GGGAAATTTG TTGAGCTTCC AGGTGCGGAG ATGGGAAAGG 
     1621 TTACCGTCAG ATTTCCTCCA GAGGCCAGTG GTTACTTACA CATTGGGCAT GCAAAAGCTG 
     1681 CTCTTCTGAA CCAGCACTAC CAGGTTAACT TTAAAGGGAA ACTGATCATG AGATTTGATG 
     1741 ACACAAATCC TGAAAAAGAA AAGGAAGATT TTGAGAAGGT TATCTTGGAA GATGTTGCAA 
     1801 TGTTGCATAT CAAACCAGAT CAATTTACTT ATACTTCGGA TCATTTTGAA ACTATAATGA 
     1861 AGTATGCAGA GAAGCTAATT CAAGAAGGGA AGGCTTATGT GGATGATACT CCTGCTGAAC 
     1921 AGATGAAAGC AGAACGTGAG CAGAGGATAG ACTCTAAACA TAGAAAAAAC CCTATTGAGA 
     1981 AGAATCTACA AATGTGGGAA GAAATGAAAA AAGGGAGCCA GTTTGGTCAG TCCTGTTGTT 
     2041 TGCGAGCAAA AATTGACATG AGTAGTAACA ATGGATGCAT GAGAGATCCA ACCCTTTATC 
     2101 GCTGCAAAAT TCAACCACAT CCAAGAACTG GAAATAAATA CAATGTTTAT CCAACATATG 
     2161 ATTTTGCCTG CCCCATAGTT GACAGCATCG AAGGTGTTAC ACATGCCCTG AGAACAACAG 
     2221 AATACCATGA CAGAGATGAG CAGTTTTACT GGATTATTGA AGCTTTAGGC ATAAGAAAAC 
     2281 CATATATTTG GGAATATAGT CGGCTAAATC TCAACAACAC AGTGCTATCC AAAAGAAAAC 
     2341 TCACATGGTT TGTCAATGAA GGACTAGTAG ATGGATGGGA TGACCCAAGA TTTCCTACGG 
     2401 TTCGTGGTGT ACTGAGAAGA GGGATGACAG TTGAAGGACT GAAACAGTTT ATTGCTGCTC 
     2461 AGGGCTCCTC ACGTTCAGTC GTGAACATGG AGTGGGACAA AATCTGGGCG TTTAACAAAA 
     2521 AGGTTATTGA CCCAGTGGCT CCACGATATG TTGCATTACT GAAGAAAGAA GTGATCCCAG 
     2581 TGAATGTACC TGAAGCTCAG GAGGAGATGA AAGAAGTAGC CAAACACCCA AAGAATCCTG 
     2641 AGGTTGGCTT GAAGCCTGTG TGGTATAGTC CCAAAGTTTT CATTGAAGGT GCTGATGCAG 
     2701 AGACTTTTTC GGAGGGTGAG ATGGTTACAT TTATAAATTG GGGCAACCTC AACATTACAA 
     2761 AAATACACAA AAATGCAGAT GGAAAAATCA TATCTCTTGA TGCAAAGTTG AATTTGGAAA 
     2821 ACAAAGACTA CAAGAAAACC ACTAAGGTCA CTTGGCTTGC AGAGACTACA CATGCTCTTC 
     2881 CTATTCCAGT AATCTGTGTC ACTTATGAGC ACTTGATCAC AAAGCCAGTG CTAGGAAAAG 
     2941 ACGAGGACTT TAAGCAGTAT GTCAACAAGA ACAGTAAGCA TGAAGAGCTA ATGCTAGGGG 
     3001 ATCCCTGCCT TAAGGATTTG AAAAAAGGAG ATATTATACA ACTCCAGAGA AGAGGATTCT 
     3061 TCATATGTGA TCAACCTTAT GAACCTGTTA GCCCATATAG TTGCAAGGAA GCCCCGTGTG 
     3121 TTTTGATATA CATTCCTGAT GGGCACACAA AGGAAATGCC AACATCAGGG TCAAAGGAAA 
     3181 AGACCAAAGT AGAAGCCACA AAAAATGAGA CCTCTGCTCC TTTTAAGGAA AGACCAACAC 
     3241 CTTCTCTGAA TAATAATTGT ACTACATCTG AGGATTCCTT GGTCCTTTAC AATAGAGTGG 
     3301 CTGTTCAAGG AGATGTGGTT CGTGAATTAA AAGCCAAGAA AGCACCAAAG GAAGATGTAG 
     3361 ATGCAGCTGT AAAACAGCTT TTGTCTTTGA AAGCTGAATA TAAGGAGAAA ACTGGCCAGG 
     3421 AATATAAACC TGGAAACCCT CCTGCTGAAA TAGGACAGAA TATTTCTTCT AATTCCTCAG 
     3481 CAAGTATTCT GGAAAGTAAA TCTCTGTATG ATGAAGTTGC TGCACAAGGG GAGGTGGTTC 
     3541 GTAAGCTAAA AGCTGAAAAA TCCCCTAAGG CTAAAATAAA TGAAGCTGTA GAATGCTTAC 
     3601 TGTCCCTGAA GGCTCAGTAT AAAGAAAAAA CTGGGAAGGA GTACATACCT GGTCAGCCCC 
     3661 CATTATCTCA AAGTTCGGAT TCAAGCCCAA CCAGAAATTC TGAACCTGCT GGTTTAGAAA 
     3721 CACCAGAAGC GAAAGTACTT TTTGACAAAG TAGCTTCTCA AGGGGAAGTA GTTCGGAAAC 
     3781 TTAAAACTGA AAAAGCCCCT AAGGATCAAG TAGATATAGC TGTTCAAGAA CTCCTTCAGC 
     3841 TAAAGGCACA GTACAAGTCT TTGATAGGAG TAGAGTATAA GCCTGTGTCG GCCACTGGAG 
     3901 CTGAGGACAA AGATAAGAAG AAGAAAGAAA AAGAAAATAA ATCTGAAAAG CAGAATAAGC 
     3961 CTCAGAAACA AAATGATGGC CAAAGGAAAG ACCCTTCTAA AAACCAAGGA GGTGGGCTCT 
     4021 CATCAAGTGG AGCAGGAGAA GGGCAGGGGC CTAAGAAACA GACCAGGTTG GGTCTTGAGG 
     4081 CAAAAAAAGA AGAAAATCTT GCTGATTGGT ATTCTCAGGT CATCACAAAG TCAGAAATGA 
     4141 TTGAATACCA TGACATAAGT GGCTGTTATA TTCTTCGTCC CTGGGCCTAT GCCATTTGGG 
     4201 AAGCCATCAA GGACTTTTTT GATGCTGAGA TCAAGAAACT TGGTGTTGAA AACTGCTACT 
     4261 TCCCCATGTT TGTGTCTCAA AGTGCATTAG AGAAAGAGAA GACTCATGTT GCTGACTTTG 
     4321 CCCCAGAGGT TGCTTGGGTT ACAAGATCTG GCAAAACCGA GCTGGCAGAA CCAATTGCCA 
     4381 TTCGTCCTAC TAGTGAAACA GTAATGTATC CTGCATATGC AAAATGGGTA CAGTCACACA 
     4441 GAGACCTGCC CATCAAGCTC AATCAGTGGT GCAATGTGGT GCGTTGGGAA TTCAAGCATC 
     4501 CTCAGCCTTT CCTACGTACT CGTGAATTTC TTTGGCAGGA AGGGCACAGT GCTTTTGCTA 
     4561 CCATGGAAGA GGCAGCGGAA GAGGTCTTGC AGATACTTGA CTTATATGCT CAGGTATATG 
     4621 AAGAACTCCT GGCAATTCCT GTTGTTAAAG GAAGAAAGAC GGAAAAGGAA AAATTTGCAG 
     4681 GAGGAGACTA TACAACTACA ATAGAAGCAT TTATATCTGC TAGTGGAAGA GCTATCCAGG 
     4741 GAGGAACATC ACATCATTTA GGGCAGAATT TTTCCAAAAT GTTTGAAATC GTTTTTGAAG 
     4801 ATCCAAAGAT ACCAGGAGAG AAGCAATTTG CCTATCAAAA CTCCTGGGGC CTGACAACTC 
     4861 GAACTATTGG TGTTATGACC ATGGTTCATG GGGACAACAT GGGTTTAGTA TTACCACCCC 
     4921 GTGTAGCATG TGTTCAGGTG GTGATTATTC CTTGTGGCAT TACCAATGCA CTTTCTGAAG 
     4981 AAGACAAAGA AGCGCTGATT GCAAAATGCA ATGATTATCG AAGGCGATTA CTCAGTGTTA 
     5041 ACATCCGCGT TAGAGCTGAT TTACGAGATA ATTATTCTCC AGGTTGGAAA TTCAATCACT 
     5101 GGGAGCTCAA GGGAGTTCCC ATTAGACTTG AAGTTGGGCC ACGTGATATG AAGAGCTGTC 
     5161 AGTTTGTAGC CGTCAGACGA GATACTGGAG AAAAGCTGAC AGTTGCTGAA AATGAGGCAG 
     5221 AGACTAAACT TCAAGCTATT TTGGAAGACA TCCAGGTCAC CCTTTTCACA AGGGCTTCTG 
     5281 AAGACCTTAA GACTCATATG GTTGTGGCTA ATACAATGGA AGACTTTCAG AAGATACTAG 
     5341 ATTCTGGAAA GATTGTTCAG ATTCCATTCT GTGGGGAAAT TGACTGTGAG GACTGGATCA 
     5401 AAAAGACCAC TGCCAGGGAT CAAGATCTTG AACCTGGTGC TCCATCCATG GGAGCTAAAA 
     5461 GCCTTTGCAT CCCCTTCAAA CCACTCTGTG AACTGCAGCC TGGAGCCAAA TGTGTCTGTG 
     5521 GCAAGAACCC TGCCAAGTAC TACACCTTAT TTGGTCGCAG CTACACGCGT ACGCGGCCGC 
     5581 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5641 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5701 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5761 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5821 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5881 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5941 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     6001 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     6061 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     6121 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     6181 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     6241 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     6301 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     6361 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     6421 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     6481 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     6541 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6601 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6661 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6721 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6781 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6841 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6901 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6961 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     7021 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     7081 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     7141 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     7201 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     7261 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     7321 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     7381 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     7441 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     7501 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     7561 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7621 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7681 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7741 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7801 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7861 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7921 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7981 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     8041 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     8101 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     8161 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     8221 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     8281 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     8341 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     8401 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     8461 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     8521 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     8581 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8641 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8701 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8761 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8821 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8881 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8941 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     9001 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     9061 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     9121 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     9181 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     9241 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     9301 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     9361 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//