LOCUS       RC217549                8010 bp    DNA     circular  SYN 
DEFINITION  GRIN3B (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, N-methyl-D-aspartate 3B (GRIN3B)
ACCESSION   NM_138690
VERSION     NM_138690.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4167..4258 
                     /label="Myc-DDK"  
     misc_feature    4288..4873 
                     /label="PolyA signal"  
     misc_feature    4345..4364 
                     /label="Restriction Enzyme"  
     misc_feature    5149..5821 
                     /label="ColE1"  
     misc_feature    6251..7045 
                     /label="Kan/Neo"  
     misc_feature    7080..7382 
                     /label="SV40 ori"  
     misc_feature    7468..7945 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4149..4155 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4149 
                     /label="GRIN3B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGTTTGTG CGGGCGCTGT GGCTGGGCCT GGCGCTGGCG CTGGGGCCGG 
     1081 GGTCCGCGGG GGGCCACCCT CAGCCGTGCG GCGTCCTGGC GCGCCTCGGG GGCTCCGTGC 
     1141 GCCTGGGCGC CCTCCTGCCC CGCGCGCCTC TCGCCCGCGC CCGCGCCCGC GCCGCCCTGG 
     1201 CCCGGGCCGC CCTGGCGCCG CGGCTGCCGC ACAACCTGAG CTTGGAGCTG GTGGTCGCCG 
     1261 CGCCCCCCGC CCGCGACCCC GCCTCGCTGA CCCGCGGCCT GTGCCAGGCG CTGGTGCCTC 
     1321 CGGGCGTGGC GGCCCTGCTC GCCTTTCCCG AGGCTCGGCC CGAGCTGCTG CAGCTGCACT 
     1381 TCCTGGCGGC GGCCACCGAG ACCCCCGTGC TCAGCCTGCT GCGGCGGGAG GCGCGCGCGC 
     1441 CCCTCGGAGC CCCGAACCCA TTCCACCTGC AGCTGCACTG GGCCAGCCCC CTGGAGACGC 
     1501 TGCTGGATGT GCTGGTGGCG GTGCTGCAGG CGCACGCCTG GGAAGACGTC GGCCTGGCCC 
     1561 TGTGCCGCAC TCAGGACCCC GGCGGCCTGG TGGCCCTCTG GACAAGCCGG GCTGGCCGGC 
     1621 CCCCACAGCT GGTCCTGGAC CTAAGCCGGC GGGACACGGG AGATGCAGGA CTGCGGGCAC 
     1681 GCCTGGCCCC GATGGCGGCG CCAGTGGGGG GTGAAGCACC GGTACCCGCG GCGGTCCTCC 
     1741 TCGGCTGTGA CATCGCCCGT GCCCGTCGGG TGCTGGAGGC CGTACCTCCC GGCCCCCACT 
     1801 GGCTGTTGGG GACACCACTG CCGCCCAAGG CCCTGCCCAC CGCGGGGCTG CCACCAGGGC 
     1861 TGCTGGCGCT GGGCGAGGTG GCACGACCCC CGCTGGAGGC CGCCATCCAT GACATTGTGC 
     1921 AACTGGTGGC CCGGGCGCTG GGCAGTGCGG CCCAGGTGCA GCCGAAGCGA GCCCTCCTCC 
     1981 CCGCCCCGGT CAACTGCGGG GACCTGCAGC CGGCCGGGCC CGAGTCCCCG GGGCGCTTCT 
     2041 TGGCACGGTT CCTGGCCAAC ACGTCCTTCC AGGGCCGCAC GGGCCCCGTG TGGGTGACAG 
     2101 GCAGCTCCCA GGTACACATG TCTCGGCACT TTAAGGTGTG GAGCCTTCGC CGGGACCCAC 
     2161 GGGGCGCCCC GGCCTGGGCC ACGGTGGGCA GCTGGCGGGA CGGCCAGCTG GACTTGGAAC 
     2221 CGGGAGGTGC CTCTGCACGG CCCCCGCCCC CACAGGGTGC CCAGGTCTGG CCCAAGCTGC 
     2281 GTGTGGTAAC GCTGTTGGAA CACCCATTTG TGTTTGCCCG TGATCCAGAC GAAGACGGGC 
     2341 AGTGCCCAGC GGGGCAGCTG TGCCTGGACC CTGGCACCAA CGACTCGGCC ACCCTGGACG 
     2401 CACTGTTCGC CGCGCTGGCC AACGGCTCAG CGCCCCGTGC CCTGCGCAAG TGCTGCTACG 
     2461 GCTACTGCAT TGACCTGCTG GAGCGGCTGG CGGAGGACAC GCCCTTCGAC TTCGAGCTGT 
     2521 ACCTCGTGGG TGACGGCAAG TACGGCGCCC TGCGGGACGG CCGCTGGACC GGCCTGGTCG 
     2581 GGGACCTGCT GGCCGGCCGG GCCCACATGG CGGTCACCAG CTTCAGTATC AACTCCGCCC 
     2641 GCTCACAGGT GGTGGACTTC ACCAGCCCCT TCTTCTCCAC CAGCCTGGGC ATCATGGTGC 
     2701 GGGCACGGGA CACGGCCTCA CCCATCGGTG CCTTTATGTG GCCCCTGCAC TGGTCCACGT 
     2761 GGCTGGGCGT CTTTGCGGCC CTGCACCTCA CCGCGCTCTT CCTCACCGTG TACGAGTGGC 
     2821 GTAGCCCCTA CGGCCTCACG CCACGTGGCC GCAACCGCAG CACCGTCTTC TCCTACTCCT 
     2881 CAGCCCTCAA CCTGTGCTAC GCCATCCTCT TCAGACGCAC CGTGTCCAGC AAGACGCCCA 
     2941 AGTGCCCCAC GGGCCGCCTG CTCATGAACC TCTGGGCCAT CTTCTGCCTG CTGGTGCTGT 
     3001 CCAGCTACAC GGCCAACCTG GCTGCCGTCA TGGTCGGGGA CAAGACCTTC GAGGAGCTGT 
     3061 CGGGGATCCA CGACCCCAAG CTGCACCACC CGGCGCAGGG CTTCCGCTTC GGCACCGTGT 
     3121 GGGAGAGCAG CGCCGAGGCG TACATCAAGA AGAGCTTCCC CGACATGCAC GCACACATGC 
     3181 GGCGCCACAG CGCGCCCACC ACGCCCCGCG GCGTCGCCAT GCTCACGAGC GACCCCCCCA 
     3241 AGCTCAACGC CTTCATCATG GACAAGTCGC TCCTGGACTA CGAGGTCTCC ATCGACGCCG 
     3301 ACTGCAAACT GCTGACCGTG GGAAAGCCCT TCGCCATTGA GGGCTATGGG ATCGGACTGC 
     3361 CCCAGAACTC GCCGCTCACC TCCAACCTGT CCGAGTTCAT CAGCCGCTAC AAGTCCTCCG 
     3421 GCTTCATCGA CCTGCTCCAC GACAAGTGGT ACAAGATGGT GCCTTGCGGC AAGCGGGTCT 
     3481 TTGCGGTTAC AGAGACCCTG CAGATGAGCA TCTACCACTT CGCGGGCCTC TTCGTGTTGC 
     3541 TGTGCCTGGG CCTGGGCAGC GCTCTGCTCA GCTCGCTGGG CGAGCACGCC TTCTTCCGCC 
     3601 TGGCGCTGCC GCGCATCCGC AAGGGGAGCA GGCTGCAGTA CTGGCTGCAC ACCAGCCAGA 
     3661 AAATCCACCG CGCCCTCAAC ACGGAGCCAC CAGAGGGGTC GAAGGAGGAG ACGGCAGAGG 
     3721 CGGAGCCCAG CGGCCCCGAG GTGGAGCAGC AGCAGCAGCA GCAGGACCAG CCAACGGCTC 
     3781 CGGAGGGCTG GAAACGGGCG CGCCGGGCCG TGGACAAGGA GCGCCGCGTG CGCTTCCTGC 
     3841 TGGAGCCCGC CGTGGTTGTG GCACCCGAAG CGGACGCGGA GGCGGAGGCT GCGCCGCGAG 
     3901 AGGGCCCCGT CTGGCTGTGC TCCTACGGCC GCCCGCCCGC CGCAAGGCCC ACGGGGGCCC 
     3961 CCCAGCCCGG GGAGCTGCAG GAGCTGGAGC GCCGCATCGA AGTCGCGCGT GAGCGGCTCC 
     4021 GCCAGGCCCT GGTGCGGCGC GGCCAGCTCC TGGCACAGCT CGGGGACAGC GCACGTCACC 
     4081 GGCCTCGGCG CTTGCTTCAG GCCAGAGCGG CCCCCGCGGA GGCCCCACCA CACTCTGGCC 
     4141 GACCGGGGAG CCAGGAAACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4201 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4261 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4321 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4381 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4441 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4501 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4561 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4621 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4681 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4741 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4801 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4861 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4921 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4981 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5041 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5101 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5161 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5221 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5281 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5341 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5401 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5461 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5521 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5581 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5641 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5701 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5761 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5821 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5881 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5941 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6001 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6061 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6121 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6181 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6241 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6301 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6361 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6421 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6481 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6541 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6601 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6661 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6721 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6781 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6841 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6901 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6961 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7021 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7081 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7141 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7201 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7261 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7321 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7381 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7441 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7501 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7561 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7621 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7681 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7741 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7801 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7861 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7921 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7981 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//