LOCUS       RC217540                7863 bp    DNA     circular  SYN 
DEFINITION  KCNH6 (Myc-DDK-tagged)-Human potassium voltage-gated channel, subfamily H (eag-related), member 6 (KCNH6), transcript variant 1
ACCESSION   NM_030779
VERSION     NM_030779.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4020..4111 
                     /label="Myc-DDK"  
     misc_feature    4141..4726 
                     /label="PolyA signal"  
     misc_feature    4198..4217 
                     /label="Restriction Enzyme"  
     misc_feature    5002..5674 
                     /label="ColE1"  
     misc_feature    6104..6898 
                     /label="Kan/Neo"  
     misc_feature    6933..7235 
                     /label="SV40 ori"  
     misc_feature    7321..7798 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4002..4008 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4002 
                     /label="KCNH6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTCCGC AGGGGCCACG TCGCTCCCCA AAACACTTAC CTGGACACCA 
     1081 TCATCCGCAA GTTCGAGGGC CAAAGTCGGA AGTTCCTGAT TGCCAATGCT CAGATGGAGA 
     1141 ACTGCGCCAT CATTTACTGC AACGACGGCT TCTGCGAACT CTTCGGCTAC TCCCGAGTGG 
     1201 AGGTGATGCA GCAACCCTGC ACCTGCGACT TCCTCACAGG CCCCAACACA CCAAGCAGCG 
     1261 CCGTGTCCCG CCTAGCGCAG GCCCTGCTGG GGGCTGAGGA GTGCAAGGTG GACATCCTCT 
     1321 ACTACCGCAA GGATGCCTCC AGCTTCCGCT GCCTGGTAGA TGTGGTGCCC GTGAAGAACG 
     1381 AGGACGGGGC TGTCATCATG TTCATTCTCA ACTTCGAGGA CCTGGCCCAG CTCCTGGCCA 
     1441 AGTGCAGCAG CCGCAGCTTG TCCCAGCGCC TGTTGTCCCA GAGCTTCCTG GGCTCCGAGG 
     1501 GCTCTCATGG CAGGCCAGGC GGACCAGGGC CAGGCACAGG CAGGGGCAAG TACAGGACCA 
     1561 TCAGCCAGAT CCCACAGTTC ACGCTCAACT TCGTGGAGTT CAACTTGGAG AAGCACCGCT 
     1621 CCAGCTCCAC CACGGAGATT GAGATCATCG CGCCCCATAA GGTGGTGGAG CGGACACAGA 
     1681 ACGTCACTGA GAAGGTCACC CAGGTCCTGT CCCTGGGCGC GGATGTGCTG CCGGAGTACA 
     1741 AGCTGCAGGC GCCGCGCATC CACCGCTGGA CCATCCTGCA CTACAGCCCC TTCAAGGCCG 
     1801 TGTGGGACTG GCTCATCCTG CTGCTGGTCA TCTACACGGC TGTCTTCACG CCCTACTCAG 
     1861 CCGCCTTCCT GCTCAGCGAT CAGGACGAAT CACGGCGTGG GGCCTGCAGC TATACCTGCA 
     1921 GTCCCCTCAC TGTGGTGGAT CTCATCGTGG ACATCATGTT CGTCGTGGAC ATCGTCATCA 
     1981 ACTTCCGCAC CACCTATGTC AACACCAATG ATGAGGTGGT CAGCCACCCC CGCCGCATCG 
     2041 CCGTCCACTA CTTCAAGGGC TGGTTCCTCA TTGACATGGT GGCCGCCATC CCTTTCGACC 
     2101 TCCTGATCTT CCGCACTGGC TCCGATGAGA CCACAACCCT GATTGGGCTA TTGAAGACAG 
     2161 CGCGGCTGCT GCGGCTGGTG CGCGTAGCAC GGAAGCTGGA CCGCTACTCT GAGTATGGGG 
     2221 CGGCTGTGCT CTTCTTGCTC ATGTGCACCT TCGCGCTCAT AGCGCACTGG CTGGCCTGCA 
     2281 TCTGGTACGC CATCGGCAAT GTGGAGCGGC CCTACCTAGA ACACAAGATC GGCTGGCTGG 
     2341 ACAGCCTGGG TGTGCAGCTT GGCAAGCGCT ACAACGGCAG CGACCCAGCC TCGGGCCCCT 
     2401 CGGTGCAGGA CAAGTATGTC ACAGCCCTCT ACTTCACCTT CAGCAGCCTC ACCAGCGTGG 
     2461 GCTTCGGCAA TGTCTCGCCC AACACCAACT CCGAGAAGGT CTTCTCCATC TGCGTCATGC 
     2521 TCATCGGCTC CCTGATGTAC GCCAGCATCT TCGGGAACGT GTCCGCGATC ATCCAGCGCC 
     2581 TGTACTCGGG CACCGCGCGC TACCACACGC AGATGCTGCG TGTCAAGGAG TTCATCCGCT 
     2641 TCCACCAGAT CCCCAACCCA CTGCGCCAGC GCCTGGAGGA GTATTTCCAG CACGCCTGGT 
     2701 CCTACACCAA TGGCATTGAC ATGAACGCGG TGCTGAAGGG CTTCCCCGAG TGCCTGCAGG 
     2761 CTGACATCTG CCTGCACCTG CACCGCGCAC TGCTGCAGCA CTGCCCAGCT TTCAGCGGCG 
     2821 CCGGCAAGGG CTGCCTGCGC GCGCTAGCCG TCAAGTTCAA GACCACCCAC GCGCCGCCTG 
     2881 GGGACACGCT GGTGCACCTC GGCGACGTGC TCTCCACCCT CTACTTCATC TCCCGAGGCT 
     2941 CCATCGAGAT CCTGCGCGAC GACGTGGTCG TGGCCATCCT AGGAAAGAAT GACATCTTTG 
     3001 GGGAACCCGT CAGCCTCCAT GCCCAGCCAG GCAAGTCCAG TGCAGACGTG CGGGCTCTGA 
     3061 CCTACTGCGA CCTGCACAAG ATCCAGCGGG CAGATCTGCT GGAGGTGCTG GACATGTACC 
     3121 CGGCCTTTGC GGAGAGCTTC TGGAGTAAGC TGGAGGTCAC CTTCAACCTG CGGGACGCAG 
     3181 CCGGGGGTCT CCACTCATCC CCCCGACAGG CTCCTGGCAG CCAAGACCAC CAAGGTTTCT 
     3241 TTCTCAGTGA CAACCAGTCA GGGTCTCCCC ATGAGCTGGG GCCCCAGTTC CCCTCTAAGG 
     3301 GCTACAGCCT CCTGGGTCCT GGGAGCCAGA ACTCCATGGG GGCAGGACCT TGTGCTCCAG 
     3361 GGCACCCAGA TGCAGCCCCT CCCCTGAGCA TCTCAGATGC ATCTGGCCTC TGGCCTGAGC 
     3421 TACTGCAGGA AATGCCCCCA AGGCACAGCC CCCAAAGCCC TCAGGAAGAC CCAGATTGCT 
     3481 GGCCTCTGAA GCTGGGCTCC AGGCTAGAGC AGCTCCAGGC CCAGATGAAC AGGCTGGAGT 
     3541 CCCGCGTGTC CTCAGACCTC AGCCGCATCT TGCAGCTCCT CCAGAAGCCC ATGCCCCAGG 
     3601 GCCACGCCAG CTACATTCTG GAAGCCCCTG CCTCCAATGA CCTGGCCTTG GTTCCTATAG 
     3661 CCTCGGAGAC GACGAGTCCA GGGCCCAGGC TGCCCCAGGG CTTTCTGCCT CCTGCACAGA 
     3721 CCCCAAGCTA TGGAGACTTG GATGACTGTA GTCCAAAGCA CAGGAACTCC TCCCCCAGGA 
     3781 TGCCTCACCT GGCTGTGGCA ACGGACAAAA CTCTGGCACC ATCCTCAGAA CAGGAACAGC 
     3841 CTGAGGGGCT CTGGCCACCC CTAGCCTCAC CTCTACATCC CCTGGAAGTA CAAGGACTCA 
     3901 TCTGTGGTCC CTGCTTCTCC TCCCTCCCTG AACACCTTGG CTCTGTTCCC AAGCAGCTGG 
     3961 ACTTCCAGAG ACATGGCTCA GATCCTGGAT TTGCAGGGAG TTGGGGCCAC ACGCGTACGC 
     4021 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4081 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4141 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4201 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4261 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4321 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     4381 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     4441 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     4501 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     4561 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     4621 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     4681 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     4741 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     4801 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     4861 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     4921 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     4981 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5041 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5101 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5161 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5221 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5281 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     5341 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     5401 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     5461 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     5521 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     5581 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     5641 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     5701 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     5761 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     5821 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     5881 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     5941 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6001 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6061 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6121 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6181 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6241 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6301 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     6361 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     6421 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     6481 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     6541 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     6601 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     6661 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     6721 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     6781 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     6841 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     6901 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     6961 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7021 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7081 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7141 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7201 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7261 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7321 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     7381 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     7441 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     7501 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     7561 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     7621 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     7681 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     7741 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     7801 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     7861 TTT
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