LOCUS       RC217528                8631 bp    DNA     circular  SYN 
DEFINITION  REV1 (Myc-DDK-tagged)-Human REV1 homolog (S. cerevisiae) (REV1), transcript variant 2
ACCESSION   NM_001037872
VERSION     NM_001037872.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4788..4879 
                     /label="Myc-DDK"  
     misc_feature    4909..5494 
                     /label="PolyA signal"  
     misc_feature    4966..4985 
                     /label="Restriction Enzyme"  
     misc_feature    5770..6442 
                     /label="ColE1"  
     misc_feature    6872..7666 
                     /label="Kan/Neo"  
     misc_feature    7701..8003 
                     /label="SV40 ori"  
     misc_feature    8089..8566 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4770..4776 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4770 
                     /label="REV1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCGAGGT GGATGGAGGA AGCGAGCTGA AAATGATGGC TGGGAAACAT 
     1081 GGGGTGGGTA TATGGCTGCC AAGGTCCAGA AATTGGAGGA ACAGTTTCGA TCAGATGCTG 
     1141 CTATGCAGAA GGATGGGACT TCATCTACAA TTTTTAGTGG AGTTGCCATC TATGTTAATG 
     1201 GATACACAGA TCCTTCCGCT GAGGAATTGA GAAAACTAAT GATGTTGCAT GGAGGTCAAT 
     1261 ACCATGTATA TTATTCCAGA TCTAAAACAA CACATATTAT TGCCACAAAT CTTCCCAATG 
     1321 CCAAAATTAA AGAATTAAAG GGGGAAAAAG TAATTCGACC AGAATGGATT GTGGAAAGCA 
     1381 TCAAAGCTGG ACGACTCCTC TCCTACATTC CATATCAGCT GTACACCAAG CAGTCCAGTA 
     1441 TGCAGAAAGG TCTCAGCTTT AATCCTGTAT GCAGACCTGA GGATCCTCTG CCAGGTCCAA 
     1501 GCAATATAGC CAAACAGCTC AACAACAGGG TAAATCACAT CGTTAAGAAG ATTGAAACGG 
     1561 AAAATGAAGT CAAAGTCAAT GGCATGAACA GTTGGAATGA AGAAGATGAA AATAATGATT 
     1621 TTAGTTTTGT GGATCTGGAG CAGACCTCTC CGGGAAGGAA ACAGAATGGA ATTCCGCATC 
     1681 CCAGAGGGAG CACTGCCATT TTTAATGGAC ACACTCCTAG CTCTAATGGT GCCTTAAAGA 
     1741 CACAGGATTG CTTGGTGCCC ATGGTCAACA GTGTTGCCAG CAGGCTTTCT CCAGCCTCTT 
     1801 CCCAGGAGGA GGATAAGGCT GAGAAGAGCA GCACTGATTT CAGAGACTGC ACTCTGCAGC 
     1861 AGTTGCAGCA AAGCACCAGA AACACAGATG CTTTGCGGAA TCCACACAGA ACTAATTCTT 
     1921 TCTCATTATC ACCTTTGCAC AGTAACACTA AAATCAATGG TGCTCACCAC TCCACTGTTC 
     1981 AGGGGCCTTC AAGCACAAAA AGCACTTCTT CAGTATCTAC GTTTAGCAAG GCAGCACCTT 
     2041 CAGTGCCATC CAAACCTTCA GACTGCAATT TTATTTCAAA CTTCTATTCT CATTCAAGAC 
     2101 TGCATCACAT ATCAATGTGG AAGTGTGAAT TGACTGAGTT TGTCAATACC CTACAAAGAC 
     2161 AAAGTAATGG TATCTTTCCA GGAAGGGAAA AGTTAAAAAA AATGAAAACA GGCAGGTCTG 
     2221 CACTTGTTGT AACTGACACA GGAGATATGT CAGTATTGAA TTCTCCCAGA CATCAGAGCT 
     2281 GTATAATGCA TGTTGATATG GATTGCTTCT TTGTATCAGT GGGTATACGA AATAGACCAG 
     2341 ATCTCAAAGG AAAACCAGTG GCTGTTACAA GTAACAGAGG CACAGGAAGG GCACCTTTAC 
     2401 GTCCTGGCGC TAACCCCCAG CTGGAGTGGC AGTATTACCA GAATAAAATC CTGAAAGGCA 
     2461 AAGCAGATAT ACCAGATTCA TCATTGTGGG AGAATCCAGA TTCTGCGCAA GCAAATGGAA 
     2521 TTGATTCTGT TTTGTCAAGG GCTGAAATTG CATCTTGTAG TTATGAGGCC AGGCAACTTG 
     2581 GCATTAAGAA CGGAATGTTT TTTGGGCATG CTAAACAACT ATGTCCTAAT CTTCAAGCTG 
     2641 TTCCATACGA TTTTCATGCA TATAAGGAAG TCGCACAAAC ATTGTATGAA ACATTGGCAA 
     2701 GCTACACTCA TAACATTGAA GCTGTCAGTT GTGATGAAGC GCTGGTAGAC ATTACCGAAA 
     2761 TCCTTGCAGA GACCAAACTT ACTCCTGATG AATTTGCAAA TGCTGTTCGT ATGGAAATCA 
     2821 AAGACCAGAC GAAATGTGCT GCCTCTGTTG GAATTGGTTC TAATATTCTC CTGGCTAGAA 
     2881 TGGCAACTAG AAAAGCAAAA CCAGATGGGC AGTACCACCT AAAACCAGAA GAAGTAGATG 
     2941 ATTTTATCAG AGGCCAGCTA GTGACCAATC TACCAGGAGT TGGACATTCA ATGGAATCTA 
     3001 AGTTGGCATC TTTGGGAATT AAAACTTGTG GAGACTTGCA GTATATGACC ATGGCAAAAC 
     3061 TCCAAAAAGA ATTTGGTCCC AAAACAGGTC AGATGCTTTA TAGGTTCTGC CGTGGCTTGG 
     3121 ATGATAGACC AGTTCGAACT GAAAAGGAAA GAAAATCTGT TTCAGCTGAG ATCAACTATG 
     3181 GAATAAGGTT TACTCAGCCA AAAGAGGCAG AAGCTTTTCT TCTGAGTCTT TCAGAAGAAA 
     3241 TTCAAAGAAG ACTAGAAGCC ACTGGCATGA AGGGTAAACG TCTAACTCTC AAAATCATGG 
     3301 TACGAAAGCC TGGGGCTCCT GTAGAAACTG CAAAATTTGG AGGCCATGGA ATTTGTGATA 
     3361 ACATTGCCAG GACTGTAACT CTTGACCAGG CAACAGATAA TGCAAAAATA ATTGGAAAGG 
     3421 CGATGCTAAA CATGTTTCAT ACAATGAAAC TAAATATATC AGATATGAGA GGGGTTGGGA 
     3481 TTCACGTGAA TCAGTTGGTT CCAACTAATC TGAACCCTTC CACATGTCCC AGTCGCCCAT 
     3541 CAGTTCAGTC AAGCCACTTT CCTAGTGGGT CATACTCTGT CCGTGATGTC TTCCAAGTTC 
     3601 AGAAAGCTAA GAAATCCACC GAAGAGGAGC ACAAAGAAGT ATTTCGGGCT GCTGTGGATC 
     3661 TGGAAATATC ATCTGCTTCT AGAACTTGCA CTTTCTTGCC ACCTTTTCCT GCACATCTGC 
     3721 CGACCAGTCC TGATACTAAC AAGGCTGAGT CTTCAGGGAA ATGGAATGGT CTACATACTC 
     3781 CTGTCAGTGT GCAGTCGAGA CTTAACCTGA GTATAGAGGT CCCGTCACCT TCCCAGCTGG 
     3841 ATCAGTCTGT TTTAGAAGCA CTTCCACCTG ATCTCCGGGA ACAAGTAGAG CAAGTCTGTG 
     3901 CTGTCCAGCA AGCAGAGTCA CATGGCGACA AAAAGAAAGA ACCAGTAAAT GGCTGTAATA 
     3961 CAGGAATTTT GCCACAACCA GTTGGGACAG TCTTGTTGCA AATACCAGAA CCTCAAGAAT 
     4021 CGAACAGTGA CGCAGGAATA AATTTAATAG CCCTTCCAGC ATTTTCACAG GTGGACCCTG 
     4081 AGGTATTTGC TGCCCTTCCT GCTGAACTTC AGAGGGAGCT GAAAGCAGCG TATGATCAAA 
     4141 GACAAAGGCA GGGCGAGAAC AGCACTCACC AGCAGTCAGC CAGCGCATCT GTGCCAAAGA 
     4201 ATCCTTTACT TCATCTAAAG GCAGCAGTGA AAGAAAAGAA AAGAAACAAG AAGAAAAAAA 
     4261 CCATCGGTTC ACCAAAAAGG ATTCAGAGTC CTTTGAATAA CAAGCTGCTT AACAGTCCTG 
     4321 CAAAAACTCT GCCAGGGGCC TGTGGCAGTC CCCAGAAGTT AATTGATGGG TTTCTAAAAC 
     4381 ATGAAGGACC TCCTGCAGAG AAACCCCTGG AAGAACTCTC TGCTTCTACT TCAGGTGTGC 
     4441 CAGGCCTTTC TAGTTTGCAG TCTGACCCAG CTGGCTGTGT GAGACCTCCA GCACCCAATC 
     4501 TAGCTGGAGC TGTTGAATTC AATGATGTGA AGACCTTGCT CAGAGAATGG ATAACTACAA 
     4561 TTTCAGATCC AATGGAAGAA GACATTCTCC AAGTTGTGAA ATACTGTACT GATCTAATAG 
     4621 AAGAAAAAGA TTTGGAAAAA CTGGATCTAG TTATAAAATA CATGAAAAGG CTGATGCAGC 
     4681 AATCGGTGGA ATCGGTTTGG AATATGGCAT TTGACTTTAT TCTTGACAAT GTCCAGGTGG 
     4741 TTTTACAACA AACTTATGGA AGCACATTAA AAGTTACAAC GCGTACGCGG CCGCTCGAGC 
     4801 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4861 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4921 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4981 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5041 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5101 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5161 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5221 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5281 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5341 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5401 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5461 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5521 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5581 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5641 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5701 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5761 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5821 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5881 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5941 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6001 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6061 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6121 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6181 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6241 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6301 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6361 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6421 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6481 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6541 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6601 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6661 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6721 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6781 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6841 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6901 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6961 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7021 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7081 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7141 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7201 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7261 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7321 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7381 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7441 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7501 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7561 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7621 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7681 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7741 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7801 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7861 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7921 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7981 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8041 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8101 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8161 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8221 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8281 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8341 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8401 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8461 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8521 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8581 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//