LOCUS       RC217487                8196 bp    DNA     circular  SYN 
DEFINITION  SH3PXD2A (Myc-DDK-tagged)-Human SH3 and PX domains 2A (SH3PXD2A)
ACCESSION   NM_014631
VERSION     NM_014631.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4353..4444 
                     /label="Myc-DDK"  
     misc_feature    4474..5059 
                     /label="PolyA signal"  
     misc_feature    4531..4550 
                     /label="Restriction Enzyme"  
     misc_feature    5335..6007 
                     /label="ColE1"  
     misc_feature    6437..7231 
                     /label="Kan/Neo"  
     misc_feature    7266..7568 
                     /label="SV40 ori"  
     misc_feature    7654..8131 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4335..4341 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4335 
                     /label="SH3PXD2A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCGCCTAC TGCGTGCAGG ATGCCACCGT GGTGGACGTG GAGAAGCGGA 
     1081 GGAACCCCTC CAAGCACTAC GTATACATAA TCAATGTGAC CTGGTCTGAC TCCACCTCCC 
     1141 AGACTATCTA CCGGAGGTAC AGCAAGTTCT TTGACCTGCA GATGCAGCTT TTGGATAAGT 
     1201 TTCCCATTGA AGGTGGCCAG AAGGACCCCA AGCAAAGGAT CATCCCCTTC CTCCCAGGCA 
     1261 AGATCCTCTT CCGCAGAAGC CACATCCGGG ACGTAGCTGT GAAGAGACTG AAGCCCATCG 
     1321 ATGAATACTG CCGGGCACTT GTCCGGCTGC CCCCCCACAT CTCACAGTGT GACGAAGTCT 
     1381 TCCGGTTCTT CGAGGCTCGA CCCGAGGATG TCAACCCTCC AAAAGAGGAC TATGGCAGTT 
     1441 CCAAGAGGAA ATCAGTGTGG CTGTCCAGCT GGGCTGAGTC GCCCAAGAAG GACGTGACAG 
     1501 GTGCCGACGC CACCGCCGAG CCCATGATCC TGGAACAGTA CGTGGTGGTG TCCAACTATA 
     1561 AGAAGCAGGA GAACTCGGAG CTGAGCCTCC AGGCCGGGGA GGTGGTGGAT GTCATCGAGA 
     1621 AGAACGAGAG CGGCTGGTGG TTCGTGAGCA CTTCTGAGGA GCAGGGCTGG GTCCCTGCCA 
     1681 CCTACCTGGA GGCCCAGAAT GGTACTCGGG ATGACTCCGA CATCAACACC TCTAAGACTG 
     1741 GAGAAGAGGA GAAGTATGTC ACCGTGCAGC CTTACACCAG CCAAAGCAAG GACGAGATTG 
     1801 GCTTTGAGAA GGGCGTCACA GTGGAGGTGA TCCGGAAGAA TCTGGAAGGC TGGTGGTATA 
     1861 TCAGATACCT GGGCAAAGAG GGCTGGGCGC CAGCATCCTA CCTGAAGAAG GCCAAGGATG 
     1921 ACCTGCCAAC CCGGAAGAAG AACCTGGCCG GCCCAGTGGA GATCATTGGG AACATCATGG 
     1981 AGATCAGCAA CCTGCTGAAC AAGAAGGCGT CTGGGGACAA GGAAACTCCA CCAGCCGAAG 
     2041 GCGAGGGCCA TGAGGCCCCC ATTGCCAAGA AGGAGATCAG CCTGCCCATC CTCTGCAATG 
     2101 CCTCCAATGG CAGTGCCGTG GGCGTTCCTG ACAGGACTGT CTCCAGGCTG GCCCAGGGCT 
     2161 CTCCAGCTGT GGCCAGGATT GCCCCTCAGC GGGCCCAGAT CAGCTCCCCG AACCTACGGA 
     2221 CAAGACCTCC ACCACGCAGA GAATCCAGCC TGGGGTTCCA ACTGCCAAAG CCACCAGAGC 
     2281 CCCCTTCTGT TGAGGTGGAG TACTACACCA TTGCCGAATT CCAGTCGTGC ATTTCCGATG 
     2341 GCATCAGCTT TCGGGGTGGA CAGAAGGCAG AGGTCATTGA TAAGAACTCA GGTGGCTGGT 
     2401 GGTACGTGCA GATCGGTGAG AAGGAGGGCT GGGCCCCCGC ATCATACATC GATAAGCGCA 
     2461 AGAAGCCCAA CCTGAGCCGC CGCACAAGCA CGCTGACCCG GCCCAAGGTG CCCCCGCCAG 
     2521 CACCCCCCAG CAAGCCCAAG GAGGCCGAGG AGGGCCCTAC GGGGGCCAGT GAGAGCCAGG 
     2581 ACTCCCCGCG GAAGCTCAAG TATGAGGAGC CTGAGTATGA CATCCCTGCA TTCGGCTTTG 
     2641 ACTCAGAGCC TGAGCTGAGC GAGGAGCCCG TGGAGGACAG AGCCTCAGGG GAGAGGCGGC 
     2701 CTGCCCAGCC CCACCGGCCC TCGCCGGCCT CTTCTCTGCA GCGGGCCCGC TTCAAGGTGG 
     2761 GTGAGTCTTC AGAGGATGTG GCCCTGGAAG AGGAGACCAT CTATGAGAAT GAGGGCTTCC 
     2821 GGCCATATGC AGAGGACACC CTGTCAGCCA GAGGCTCCTC CGGGGACAGC GACTCCCCAG 
     2881 GCAGCTCCTC GCTGTCCCTG ACCAGGAAAA ACTCCCCCAA ATCAGGCTCC CCCAAGTCAT 
     2941 CATCACTCCT AAAGCTCAAG GCAGAGAAGA ATGCCCAGGC AGAAATGGGG AAGAACCACT 
     3001 CCTCAGCCTC CTTTTCCTCA TCCATCACCA TCAACACCAC TTGCTGCTCC TCCTCTTCCT 
     3061 CCTCCTCCTC TTCCTTGTCC AAAACCAGTG GCGACCTGAA GCCCCGCTCT GCTTCGGACG 
     3121 CAGGCATCCG CGGCACTCCC AAGGTCAGGG CAAAGAAGGA TGCTGATGCG AACGCTGGGC 
     3181 TGACCTCCTG TCCCCGGGCC AAGCCATCGG TCCGGCCCAA GCCATTCCTA AACCGAGCAG 
     3241 AGTCGCAGAG CCAAGAGAAG ATGGACATCA GCACTTTACG GCGCCAGCTG AGACCCACAG 
     3301 GCCAGCTCCG TGGAGGGCTC AAGGGCTCCA AGAGTGAGGA TTCGGAGCTG CCCCCGCAGA 
     3361 CGGCCTCCGA GGCTCCCAGT GAGGGGTCTA GGAGAAGCTC ATCCGACCTC ATCACCCTCC 
     3421 CAGCCACCAC TCCCCCATGT CCCACCAAGA AGGAATGGGA AGGGCCAGCC ACCTCGTACA 
     3481 TGACATGCAG CGCCTACCAG AAGGTCCAGG ACTCGGAGAT CAGCTTCCCC GCGGGCGTGG 
     3541 AGGTGCAGGT GCTGGAGAAG CAGGAGAGCG GGTGGTGGTA TGTGAGGTTT GGGGAGCTGG 
     3601 AGGGCTGGGC CCCTTCCCAC TATTTGGTGC TGGATGAGAA CGAGCAACCT GACCCCTCTG 
     3661 GCAAAGAGCT GGACACAGTG CCCGCCAAGG GCAGGCAGAA CGAAGGCAAA TCAGACAGCC 
     3721 TGGAGAAGAT CGAGAGGCGC GTCCAAGCAC TGAACACCGT CAACCAGAGC AAGAAGGCCA 
     3781 CGCCCCCCAT CCCCTCCAAA CCTCCCGGGG GCTTCGGCAA GACCTCAGGC ACTCCAGCGG 
     3841 TGAAGATGAG GAACGGAGTG CGGCAGGTGG CGGTCAGGCC CCAGTCGGTG TTTGTGTCCC 
     3901 CGCCACCCAA GGACAACAAC CTGTCCTGCG CCCTGCGGAG GAATGAGTCA CTCACGGCCA 
     3961 CTGATGGCCT CCGAGGCGTC CGACGGAACT CCTCCTTTAG CACTGCTCGC TCCGCTGCCG 
     4021 CCGAGGCCAA GGGCCGCCTG GCCGAACGGG CTGCCAGCCA GGGTTCAGAC TCACCCCTAC 
     4081 TGCCCGCCCA GCGCAACAGC ATACCCGTGT CCCCTGTGCG CCCCAAGCCC ATCGAGAAGT 
     4141 CTCAGTTCAT CCACAATAAC CTCAAAGATG TGTACGTCTC TATCGCAGAC TACGAGGGGG 
     4201 ATGAGGAGAC AGCAGGCTTC CAGGAGGGGG TGTCCATGGA GGTTCTGGAG AGGAACCCTA 
     4261 ATGGCTGGTG GTACTGCCAG ATCCTGGATG GTGTGAAGCC CTTCAAAGGC TGGGTGCCTT 
     4321 CCAACTACCT TGAGAAAAAG AACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4381 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4441 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4501 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4561 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4621 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4681 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4741 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4801 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4861 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4921 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4981 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5041 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5101 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5161 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5221 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5281 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5341 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5401 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5461 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5521 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5581 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5641 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5701 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5761 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5821 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5881 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5941 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6001 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6061 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6121 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6181 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6241 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6301 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6361 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6421 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6481 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6541 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6601 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6661 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6721 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6781 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6841 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6901 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6961 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7021 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7081 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7141 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7201 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7261 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7321 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7381 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7441 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7501 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7561 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7621 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7681 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7741 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7801 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7861 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7921 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7981 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8041 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8101 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8161 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//