LOCUS       RC217471                7230 bp    DNA     circular  SYN 
DEFINITION  CEP89 (Myc-DDK-tagged)-Human centrosomal protein 89kDa (CEP89)
ACCESSION   NM_032816
VERSION     NM_032816.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3387..3478 
                     /label="Myc-DDK"  
     misc_feature    3508..4093 
                     /label="PolyA signal"  
     misc_feature    3565..3584 
                     /label="Restriction Enzyme"  
     misc_feature    4369..5041 
                     /label="ColE1"  
     misc_feature    5471..6265 
                     /label="Kan/Neo"  
     misc_feature    6300..6602 
                     /label="SV40 ori"  
     misc_feature    6688..7165 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3369..3375 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3369 
                     /label="CEP89"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCCTGGGA TTTCGGAGAG GCCGCAGGAG TCATTTCAAA CACATCATCC 
     1081 ATGGCCTTTT ACCTGCAGCC AGCGTTGCTC CGAAGGCAGC TGTGCCACGC ACACCTCCTC 
     1141 CCCGCAGCCC CAACCCATCT CCAGAGAGAC CAAGATCTGC TCTGGCAGCA GCCATTCTGG 
     1201 CGACAACATT GACTGGGCGG ACGGTTGCTA TTCCTCAGCC TCGCCAGAGG TCCCGGTCTG 
     1261 AGAGTGATGT GAGCAGTGTT GAACAGGACA GCTTCATCGA GCCCTATGCC ACCACCTCAC 
     1321 AGCTGAGGCC TCGGCCAAAT TGGCAGAGTG AGATGGGAAG AAGATCTTCT TTGCCATCCT 
     1381 TTGAAACACT GGACTATGGG GACGAAGAGG ACATTGAAAC TCAGCTGTCA TCCAGCGGCA 
     1441 AGGAATTGGG GGATGTCAGT GCCCGGGAGG ACAGAGGAGG CCACAGTGAT GACCTGTACG 
     1501 CTGTGCCACA CAGAAATCAG GTGCCATTGT TACATGAGGT GAACAGTGAA GACGATGAAA 
     1561 ATATTTCTCA TCAAGATGGG TTTCCAGGCT CCCCTCCTGC ACCACAGCGG ACACAACAAA 
     1621 AAGATGGTAA ACACCCTGTT CTGAATTTAA AGGATGAAAA ACCTCCATTA TGTGAGAAAC 
     1681 CTCCACCCTC CCCAGATATA ACTGGTAGAG CACGTCAAAG ATATACAGAA ATAACCAGAG 
     1741 AAAAGTTTGA GGCATTAAAA GAAGAAAATA TGGACCTAAA CAATATGAAT CAAAGCCTTA 
     1801 CCCTTGAACT AAACACAATG AAACAAGCAA TGAAAGAACT ACAGTTAAAA CTTAAGGGAA 
     1861 TGGAAAAAGA GAAGAGAAAG CTCAAAGAGG CTGAGAAGGC GTCGTCACAG GAAGTTGCTG 
     1921 CACCTGAATT ACTTTATCTG CGAAAACAAG CTCAAGAACT GGTGGATGAA AATGATGGAT 
     1981 TGAAAATGAC TGTCCATCGT TTGAATGTAG AACTCAGTCG ATATCAGACA AAATTCAGGC 
     2041 ATTTGTCCAA GGAAGAGAGC TTAAATATTG AAGGCCTCCC ATCCAAGGGC CCTATACCAC 
     2101 CCTGGTTGTT GGATATAAAG TACCTGTCAC CATTGTTGCT GGCTTATGAA GATATGATGA 
     2161 AAGAGAAGGA CGAGCTCAAT GCCACCCTCA AGGAGGAAAT GAGAATGTTT AGGATGCGAG 
     2221 TCCAAGAAGT GGTGAAAGAA AATGAAGAAT TGCACCAAGA GTTAAATAAG AGTAGTGCTG 
     2281 TTACCAGTGA GGAATGGCGT CAGCTTCAGA CTCAAGCAAA ACTGGTTTTA GAGGAAAACA 
     2341 AGTTGTTGCT GGAGCAGTTG GAGATTCAGC AAAGGAAAGC CAAGGACAGC CACCAGGAGC 
     2401 GCCTCCAAGA AGTTTCTAAG CTGACTAAAC AACTAATGCT CCTGGAGGCA AAAACCCACG 
     2461 GCCAGGAAAA GGAGCTGGCG GAGAACAGGG AACAGCTGGA GATTTTACGT GCCAAATGCC 
     2521 AAGAACTCAA AACACACTCG GATGGCAAAA TCGCAGTGGA AGTTCATAAA TCAATTGTGA 
     2581 ATGAATTAAA AAGCCAATTA CAGAAGGAAG AAGAGAAAGA AAGGGCTGAG ATGGAGGAGT 
     2641 TGATGGAGAA GCTGACAGTC CTGCAAGCGC AGAAGAAGAG CCTGCTGTTA GAGAAGAACA 
     2701 GTTTGACAGA GCAAAACAAA GCACTGGAAG CCGAACTTGA ACGAGCACAG AAAATCAATA 
     2761 GGAAATCTCA AAAGAAAATT GAGGTCCTCA AAAAGCAGGT GGAAAAAGCC ATGGGGAACG 
     2821 AAATGTCTGC TCATCAGTAC CTGGCAAACC TTGTTGGCCT GGCAGAAAAT ATAACCCAGG 
     2881 AACGTGACAG TCTTATGTGT TTGGCAAAAT GTTTAGAAAG TGAGAAGGAT GGAGTGCTTA 
     2941 ATAAAGTCAT AAAAAGCAAC ATTCGCCTGG GAAAGTTAGA GGAAAAAGTC AAGGGCTACA 
     3001 AGAAGCAGGC AGCACTGAAG CTGGGGGACA TCAGTCACCG TCTGCTGGAG CAGCAGGAGG 
     3061 ACTTCGCCGG CAAGACAGCC CAGTACCGGC AGGAGATGCG GCACCTGCAC CAGGTGCTGA 
     3121 AGGACAAGCA GGAGGTGCTG GACCAGGCGC TGCAGCAGAA CAGAGAAATG GAAGGTGAAC 
     3181 TTGAAGTTAT TTGGGAATCT ACCTTCAGGG AAAACCGAAG AATCCGAGAA CTTCTCCAGG 
     3241 ACACACTCAC GAGGACAGGC GTGCAGGACA ACCCCAGAGC TCTGGTTGCC CCCAGCCTCA 
     3301 ATGGCGTCTC TCAGGCAGAC CTGCTGGACG GCTGCGATGT CTGCTCCTAT GACCTGAAGT 
     3361 CTCATGCCCC CACCTGCACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3421 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     3481 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     3541 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     3601 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     3661 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     3721 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     3781 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     3841 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     3901 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     3961 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4021 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4081 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4141 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4201 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4261 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4321 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4381 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4441 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     4501 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     4561 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     4621 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     4681 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     4741 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     4801 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     4861 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     4921 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     4981 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5041 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5101 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5161 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5221 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5281 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5341 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5401 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     5461 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     5521 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     5581 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     5641 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     5701 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     5761 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     5821 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     5881 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     5941 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6001 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6061 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6121 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6181 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6241 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6301 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6361 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6421 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     6481 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     6541 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6601 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     6661 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     6721 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     6781 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     6841 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     6901 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     6961 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7021 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7081 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7141 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7201 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//