LOCUS       RC217410                8754 bp    DNA     circular  SYN 
DEFINITION  MAP3K6 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 6 (MAP3K6)
ACCESSION   NM_004672
VERSION     NM_004672.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4911..5002 
                     /label="Myc-DDK"  
     misc_feature    5032..5617 
                     /label="PolyA signal"  
     misc_feature    5089..5108 
                     /label="Restriction Enzyme"  
     misc_feature    5893..6565 
                     /label="ColE1"  
     misc_feature    6995..7789 
                     /label="Kan/Neo"  
     misc_feature    7824..8126 
                     /label="SV40 ori"  
     misc_feature    8212..8689 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4893..4899 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4893 
                     /label="MAP3K6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG GCGGGGCCGT GTCCCCGGTC CGGGGCGGAG CGCGCCGGCA 
     1081 GCTGCTGGCA GGACCCGCTG GCCGTGGCGC TGAGCCGGGG CCGGCAGCTC GCGGCGCCCC 
     1141 CGGGCCGGGG CTGCGCGCGG AGCCGGCCGC TCAGCGTGGT CTACGTGCTG ACCCGGGAGC 
     1201 CGCAGCCCGG GCTCGAGCCT CGGGAGGGAA CCGAGGCGGA GCCGCTGCCC CTGCGCTGCC 
     1261 TGCGCGAGGC TTGCGCGCAG GTCCCCCGGC CGCGGCCGCC CCCGCAGCTG CGCAGCCTGC 
     1321 CCTTCGGGAC GCTGGAGCTA GGCGACACCG CGGCTCTGGA TGCCTTCTAC AACGCGGATG 
     1381 TGGTGGTGCT GGAGGTGAGC AGCTCGCTGG TACAGCCCTC CCTGTTCTAC CACCTTGGTG 
     1441 TGCGTGAGAG CTTCAGCATG ACCAACAATG TGCTCCTCTG CTCCCAGGCC GACCTCCCTG 
     1501 ACCTGCAGGC CCTGCGGGAG GATGTTTTCC AGAAGAACTC GGATTGCGTT GGCAGCTACA 
     1561 CACTGATCCC CTATGTGGTG ACGGCCACTG GTCGGGTGCT GTGTGGTGAT GCAGGCCTTC 
     1621 TGCGGGGCCT GGCTGATGGG CTGGTACAGG CTGGAGTGGG GACCGAGGCC CTGCTCACTC 
     1681 CCCTGGTGGG CCGGCTTGCC CGCCTGCTGG AGGCCACACC CACAGACTCT TGTGGCTATT 
     1741 TCCGGGAGAC CATTCGGCGG GACATCCGGC AGGCGCGGGA GCGGTTCAGT GGGCCACAGC 
     1801 TGCGGCAGGA GCTGGCTCGC CTGCAGCGGA GACTGGACAG CGTGGAGCTG CTGAGCCCCG 
     1861 ACATCATCAT GAACTTGCTG CTCTCCTACC GCGATGTGCA GGACTACTCG GCCATCATTG 
     1921 AGCTGGTGGA GACGCTGCAG GCCTTGCCCA CCTGTGATGT GGCCGAGCAG CATAATGTCT 
     1981 GCTTCCACTA CACTTTTGCC CTCAACCGGA GGAACAGGCC TGGGGACCGG GCGAAGGCCC 
     2041 TGTCTGTGCT GCTGCCGCTG GTACAGCTTG AGGGCTCTGT GGCGCCCGAT CTGTACTGCA 
     2101 TGTGTGGCCG TATCTACAAG GACATGTTCT TCAGCTCGGG TTTCCAGGAT GCTGGGCACC 
     2161 GGGAGCAGGC CTATCACTGG TATCGCAAGG CTTTTGACGT AGAGCCCAGC CTTCACTCAG 
     2221 GCATCAATGC AGCTGTGCTC CTCATTGCTG CCGGGCAGCA CTTTGAGGAT TCCAAAGAGC 
     2281 TCCGGCTAAT AGGCATGAAG CTGGGCTGCC TGCTGGCCCG CAAAGGCTGC GTGGAGAAGA 
     2341 TGCAGTATTA CTGGGATGTG GGTTTCTACC TGGGAGCCCA GATCCTCGCC AATGACCCCA 
     2401 CCCAGGTGGT GCTGGCTGCA GAGCAGCTGT ATAAGCTCAA TGCCCCCATA TGGTACCTGG 
     2461 TGTCCGTGAT GGAGACCTTC CTGCTCTACC AGCACTTCAG GCCCACGCCA GAGCCCCCTG 
     2521 GAGGGCCACC ACGCCGTGCC CACTTCTGGC TCCACTTCTT GCTACAGTCC TGCCAACCAT 
     2581 TCAAGACAGC CTGTGCCCAG GGCGACCAGT GCTTGGTGCT GGTCCTGGAG ATGAACAAGG 
     2641 TGCTGCTGCC TGCAAAGCTC GAGGTTCGGG GTACTGACCC AGTAAGCACA GTGACCCTGA 
     2701 GCCTGCTGGA GCCTGAGACC CAGGACATTC CCTCCAGCTG GACCTTCCCA GTCGCCTCCA 
     2761 TATGCGGAGT CAGCGCCTCA AAGCGCGACG AGCGCTGCTG CTTCCTCTAT GCACTCCCCC 
     2821 CGGCTCAGGA CGTCCAGCTG TGCTTCCCCA GCGTAGGGCA CTGCCAGTGG TTCTGCGGCC 
     2881 TGATCCAGGC CTGGGTGACG AACCCGGATT CCACGGCGCC CGCGGAGGAG GCGGAGGGCG 
     2941 CGGGGGAGAT GTTGGAGTTT GATTATGAGT ACACGGAGAC GGGCGAGCGG CTGGTGCTGG 
     3001 GCAAGGGCAC GTATGGGGTG GTGTACGCGG GCCGCGATCG CCACACGAGG GTGCGCATCG 
     3061 CCATCAAGGA GATCCCGGAG CGGGACAGCA GGTTCTCTCA GCCCCTGCAT GAAGAGATCG 
     3121 CTCTTCACAG ACGCCTGCGC CACAAGAACA TAGTGCGCTA TCTGGGCTCA GCTAGCCAGG 
     3181 GCGGCTACCT TAAGATCTTC ATGGAGGAAG TGCCTGGAGG CAGCCTGTCC TCCTTGCTGC 
     3241 GGTCGGTGTG GGGACCCCTG AAGGACAACG AGAGCACCAT CAGTTTCTAC ACCCGCCAGA 
     3301 TCCTGCAGGG ACTTGGCTAC TTGCACGACA ACCACATCGT GCACAGGGAC ATAAAAGGGG 
     3361 ACAATGTGCT GATCAACACC TTCAGTGGGC TGCTCAAGAT TTCTGACTTC GGCACCTCCA 
     3421 AGCGGCTGGC AGGCATCACA CCTTGCACTG AGACCTTCAC AGGAACTCTG CAGTATATGG 
     3481 CCCCAGAAAT CATTGACCAG GGCCCACGCG GGTATGGGAA AGCAGCTGAC ATCTGGTCAC 
     3541 TGGGCTGCAC TGTCATTGAG ATGGCCACAG GTCGCCCCCC CTTCCACGAG CTCGGGAGCC 
     3601 CACAGGCTGC CATGTTTCAG GTGGGTATGT ACAAGGTCCA TCCGCCAATG CCCAGCTCTC 
     3661 TGTCGGCCGA GGCCCAAGCC TTTCTCCTCC GAACTTTTGA GCCAGACCCC CGCCTCCGAG 
     3721 CCAGCGCCCA GACACTGCTG GGGGACCCCT TCCTGCAGCC TGGGAAAAGG AGCCGCAGCC 
     3781 CCAGCTCCCC ACGACATGCT CCACGGCCCT CAGATGCCCC TTCTGCCAGT CCCACTCCTT 
     3841 CAGCCAACTC AACCACCCAG TCTCAGACAT TCCCGTGCCC TCAGGCACCC TCTCAGCACC 
     3901 CACCCAGCCC CCCGAAGCGC TGCCTCAGTT ATGGGGGCAC CAGCCAGCTC CGGGTGCCCG 
     3961 AGGAGCCTGC GGCCGAGGAG CCTGCGTCTC CGGAGGAGAG TTCGGGGCTG AGCCTGCTGC 
     4021 ACCAGGAGAG CAAGCGTCGG GCCATGCTGG CCGCAGTATT GGAGCAGGAG CTGCCAGCGC 
     4081 TGGCGGAGAA TCTGCACCAG GAGCAGAAGC AAGAGCAGGG GGCCCGTCTG GGCAGAAACC 
     4141 ATGTGGAAGA GCTGCTGCGC TGCCTCGGGG CACACATCCA CACTCCCAAC CGCCGGCAGC 
     4201 TCGCCCAGGA GCTGCGGGCG CTGCAAGGAC GGCTGAGGGC CCAGGGCCTT GGGCCTGCGC 
     4261 TTCTGCACAG ACCGCTGTTT GCCTTCCCGG ATGCGGTGAA GCAGATCCTC CGCAAGCGCC 
     4321 AGATCCGTCC ACACTGGATG TTCGTTCTGG ACTCACTGCT CAGCCGTGCT GTGCGGGCAG 
     4381 CCCTGGGTGT GCTAGGACCG GAGGTGGAGA AGGAGGCGGT CTCACCGAGG TCAGAGGAGC 
     4441 TGAGTAATGA AGGGGACTCC CAGCAGAGCC CAGGCCAGCA GAGCCCGCTT CCGGTGGAGC 
     4501 CCGAGCAGGG CCCCGCTCCT CTGATGGTGC AGCTGAGCCT CTTGAGGGCA GAGACTGATC 
     4561 GGCTGCGCGA AATCCTGGCG GGGAAGGAAC GGGAGTACCA GGCCCTGGTG CAGCGGGCTC 
     4621 TACAGCGGCT GAATGAGGAA GCCCGGACCT ATGTCCTGGC CCCAGAGCCT CCAACTGCTC 
     4681 TTTCAACGGA CCAGGGCCTG GTGCAGTGGC TACAGGAACT GAATGTGGAT TCAGGCACCA 
     4741 TCCAAATGCT GTTGAACCAT AGCTTCACCC TCCACACTCT GCTCACCTAT GCCACTCGAG 
     4801 ATGACCTCAT CTACACCCGC ATCAGGGGAG GGATGGTATG CCGCATCTGG AGGGCCATCT 
     4861 TGGCACAGCG AGCAGGATCC ACACCAGTCA CCTCTGGACC CACGCGTACG CGGCCGCTCG 
     4921 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4981 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5041 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5101 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5161 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5221 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5281 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5341 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5401 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5461 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5521 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5581 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5641 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5701 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5761 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5821 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5881 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5941 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6001 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6061 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6121 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6181 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6241 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6301 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6361 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6421 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6481 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6541 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6601 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6661 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6721 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6781 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6841 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6901 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6961 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7021 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7081 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7141 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7201 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7261 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7321 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7381 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7441 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7501 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7561 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7621 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7681 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7741 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7801 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7861 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7921 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7981 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8041 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8101 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8161 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8221 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8281 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8341 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8401 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8461 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8521 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8581 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8641 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8701 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//