LOCUS       RC217391                8865 bp    DNA     circular  SYN 
DEFINITION  MAP3K19 (Myc-DDK-tagged)-Human YSK4 Sps1/Ste20-related kinase homolog (S. cerevisiae) (YSK4), transcript variant 1
ACCESSION   NM_025052
VERSION     NM_025052.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5022..5113 
                     /label="Myc-DDK"  
     misc_feature    5143..5728 
                     /label="PolyA signal"  
     misc_feature    5200..5219 
                     /label="Restriction Enzyme"  
     misc_feature    6004..6676 
                     /label="ColE1"  
     misc_feature    7106..7900 
                     /label="Kan/Neo"  
     misc_feature    7935..8237 
                     /label="SV40 ori"  
     misc_feature    8323..8800 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5004..5010 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5004 
                     /label="MAP3K19"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTTCTATG CCAAAACCAG AAAGACATGC TGAGTCATTG CTTGACATTT 
     1081 GTCATGATAC AAACTCTTCT CCAACTGATT TGATGACAGT TACCAAAAAT CAAAACATCA 
     1141 TCTTGCAAAG CATCAGCAGA AGTGAGGAGT TCGACCAAGA TGGTGACTGC AGTCATTCCA 
     1201 CACTGGTTAA TGAAGAAGAA GATCCCAGTG GTGGTAGACA GGACTGGCAA CCCAGGACAG 
     1261 AAGGTGTTGA GATCACTGTA ACTTTTCCAA GAGATGTCAG TCCTCCCCAA GAAATGAGCC 
     1321 AAGAAGACTT AAAAGAAAAG AATCTGATAA ACTCATCGCT TCAAGAATGG GCACAAGCAC 
     1381 ATGCAGTTTC TCATCCAAAT GAAATAGAAA CGGTGGAGCT CAGGAAAAAG AAGCTGACCA 
     1441 TGCGGCCCTT AGTTTTGCAA AAAGAGGAAA GTTCCAGGGA GCTCTGCAAT GTGAACTTGG 
     1501 GCTTTTTGCT ACCAAGATCT TGTTTAGAAC TGAACATTTC CAAGTCTGTA ACCAGAGAAG 
     1561 ATGCTCCTCA TTTTCTGAAG GAGCAGCAAA GAAAATCTGA AGAGTTTTCG ACCTCTCATA 
     1621 TGAAGTACAG TGGCCGAAGC ATCAAGTTCC TTCTGCCACC ACTGTCACTC TTGCCCACGC 
     1681 GATCTGGTGT CCTTACTATC CCCCAAAATC ACAAGTTTCC AAAAGAAAAA GAAAGAAACA 
     1741 TTCCAAGTCT CACATCTTTT GTGCCTAAGC TCTCAGTGTC TGTTCGTCAA TCTGATGAGC 
     1801 TCAGCCCATC AAACGAGCCT CCGGGAGCCC TAGTTAAGTC GTTGATGGAT CCGACTCTCA 
     1861 GGTCTTCTGA TGGCTTCATT TGGTCAAGAA ACATGTGCTC TTTTCCTAAG ACTAACCATC 
     1921 ACAGGCAATG CCTGGAGAAG GAGGAAAACT GGAAATCCAA GGAAATAGAA GAATGTAACA 
     1981 AAATTGAAAT CACTCACTTT GAAAAAGGGC AGTCTTTGGT GTCTTTTGAG AATTTGAAGG 
     2041 AAGGCAATAT TCCTGCAGTT AGGGAAGAGG ATATTGACTG CCATGGTAGT AAAACGCGAA 
     2101 AACCTGAAGA AGAGAACTCT CAATATCTTT CATCAAGAAA GAATGAGAGT TCAGTAGCCA 
     2161 AAAACTATGA ACAAGATCCA GAAATAGTAT GTACCATTCC AAGCAAGTTC CAAGAAACCC 
     2221 AGCATTCAGA AATAACTCCA AGCCAGGATG AAGAGATGAG AAATAATAAA GCTGCTTCAA 
     2281 AAAGAGTTTC ATTACATAAA AATGAAGCAA TGGAACCAAA CAATATTTTA GAAGAGTGTA 
     2341 CTGTACTTAA AAGCTTATCC AGTGTAGTCT TTGATGACCC CATTGATAAA CTCCCAGAAG 
     2401 GTTGTAGCAG CATGGAGACA AACATAAAAA TATCAATAGC AGAAAGAGCC AAACCAGAAA 
     2461 TGAGTAGGAT GGTGCCTCTT ATCCACATCA CCTTCCCTGT GGATGGAAGC CCCAAGGAAC 
     2521 CAGTGATAGC CAAACCAAGC CTCCAAACAA GAAAGGGAAC CATTCATAAC AACCATAGTG 
     2581 TCAACATACC TGTACACCAA GAAAATGACA AGCATAAGAT GAATTCCCAT AGGAGTAAGT 
     2641 TGGATTCAAA GACCAAGACA AGTAAGAAGA CACCTCAGAA TTTTGTGATT TCTACTGAAG 
     2701 GTCCCATTAA GCCTACCATG CATAAAACCA GCATAAAAAC ACAAATTTTC CCGGCTTTGG 
     2761 GACTTGTGGA CCCCAGGCCT TGGCAATTGC CCAGGTTTCA AAAGAAAATG CCACAGATAG 
     2821 CAAAGAAGCA ATCAACTCAC CGGACTCAGA AACCTAAAAA GCAATCATTT CCTTGCATCT 
     2881 GTAAAAATCC AGGAACACAG AAGTCATGTG TTCCTCTCTC TGTTCAACCG ACAGAGCCAA 
     2941 GACTAAATTA CCTAGATCTT AAGTATAGTG ATATGTTCAA AGAAATCAAT TCAACTGCTA 
     3001 ATGGACCTGG AATCTATGAA ATGTTTGGGA CCCCTGTTTA TTGTCATGTG CGAGAGACTG 
     3061 AAAGGGATGA AAACACGTAT TACCGTGAGA TATGTTCGGC TCCATCAGGC AGACGTATCA 
     3121 CCAATAAATG TCGATCTTCA CACAGTGAGA GGAAGAGCAA TATCAGAACA AGACTTTCTC 
     3181 AGAAAAAAAC ACATATGAAA TGCCCAAAGA CTTCATTTGG CATTAAACAA GAGCACAAAG 
     3241 TCTTAATTTC TAAAGAAAAG AGTTCCAAGG CTGTACATAG CAACCTACAT GACATTGAAA 
     3301 ATGGTGATGG TATTTCAGAA CCAGACTGGC AGATAAAGTC TTCAGGAAAT GAGTTTCTAT 
     3361 CTTCCAAAGA TGAAATTCAT CCCATGAACT TGGCTCAGAC ACCTGAGCAG TCCATGAAAC 
     3421 AGAATGAATT CCCTCCTGTC TCAGATTTAT CCATTGTTGA AGAAGTTTCT ATGGAAGAGT 
     3481 CTACTGGTGA TAGAGACATT TCTAACAATC AAATACTCAC CACAAGCCTC AGAGATCTGC 
     3541 AAGAACTTGA AGAGCTACAT CACCAGATCC CATTTATCCC TTCAGAAGAC AGCTGGGCAG 
     3601 TGCCCAGTGA GAAGAATTCT AACAAGTATG TACAGCAAGA AAAGCAGAAT ACAGCATCTC 
     3661 TTAGTAAAGT AAATGCCAGC CGAATTTTAA CTAATGATCT AGAGTTTGAT AGTGTTTCAG 
     3721 ATCACTCTAA AACACTTACA AATTTCTCTT TCCAAGCAAA ACAAGAAAGT GCATCTTCCC 
     3781 AGACATATCA ATATTGGGTA CATTATTTGG ATCATGATAG TTTAGCAAAT AAGTCAATCA 
     3841 CATATCAAAT GTTTGGAAAA ACCTTAAGTG GCACAAATTC AATTTCCCAA GAAATTATGG 
     3901 ACTCTGTAAA TAATGAAGAA TTGACAGATG AACTATTAGG TTGTCTAGCT GCAGAATTAT 
     3961 TAGCTCTTGA TGAGAAAGAT AACAACTCTT GCCAAAAAAT GGCAAATGAA ACAGATCCTG 
     4021 AAAACCTAAA TCTTGTCCTC AGATGGAGAG GAAGTACCCC AAAAGAAATG GGCAGAGAGA 
     4081 CAACAAAAGT CAAAATACAG AGGCATAGTA GTGGGCTCAG GATATATGAC AGGGAGGAGA 
     4141 AATTTCTCAT CTCAAATGAA AAGAAGATAT TTTCTGAAAA TAGTTTAAAG TCTGAAGAAC 
     4201 CTATCCTATG GACCAAGGGT GAGATTCTTG GAAAGGGAGC CTACGGCACA GTATACTGTG 
     4261 GTCTCACTAG TCAAGGACAG CTAATAGCTG TAAAACAGGT GGCTTTGGAT ACCTCTAATA 
     4321 AATTAGCTGC TGAAAAGGAA TACCGGAAAC TACAGGAAGA AGTAGATTTG CTCAAAGCAC 
     4381 TGAAACATGT CAACATTGTG GCCTATTTGG GGACATGCTT GCAAGAGAAC ACTGTGAGCA 
     4441 TTTTCATGGA GTTTGTTCCT GGTGGCTCAA TCTCTAGTAT TATAAACCGT TTTGGGCCAT 
     4501 TGCCTGAGAT GGTGTTCTGT AAATATACGA AACAAATACT TCAAGGTGTT GCTTATCTCC 
     4561 ATGAGAACTG TGTGGTACAT CGCGATATCA AAGGAAATAA TGTTATGCTC ATGCCAACTG 
     4621 GAATAATAAA GCTGATTGAC TTTGGCTGTG CCAGGCGTTT GGCCTGGGCA GGTTTAAATG 
     4681 GCACCCACAG TGACATGCTT AAGTCCATGC ATGGGACTCC ATATTGGATG GCCCCAGAAG 
     4741 TCATCAATGA GTCTGGCTAT GGACGGAAAT CAGATATCTG GAGCATTGGT TGTACTGTGT 
     4801 TTGAGATGGC TACAGGGAAG CCTCCACTGG CTTCCATGGA CAGGATGGCC GCCATGTTTT 
     4861 ACATCGGAGC ACACCGAGGG CTGATGCCTC CTTTACCAGA CCACTTCTCA GAAAATGCAG 
     4921 CAGACTTTGT GCGCATGTGC CTGACCAGGG ACCAGCATGA GCGACCTTCT GCTCTCCAGC 
     4981 TCCTGAAGCA CTCCTTCTTG GAGAGAAGTC ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5041 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5101 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5161 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5221 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5281 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5341 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5401 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5461 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5521 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5581 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5641 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5701 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5761 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5821 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5881 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5941 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6001 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6061 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6121 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6181 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6241 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6301 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6361 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6421 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6481 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6541 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6601 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6661 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6721 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6781 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6841 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6901 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6961 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7021 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7081 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7141 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7201 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7261 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7321 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7381 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7441 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7501 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7561 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7621 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7681 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7741 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7801 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7861 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7921 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7981 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8041 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8101 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8161 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8221 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8281 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8341 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8401 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8461 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8521 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8581 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8641 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8701 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8761 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8821 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//