LOCUS       RC217385                8994 bp    DNA     circular  SYN 
DEFINITION  ADAMTS13 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 13 (ADAMTS13), transcript variant 2
ACCESSION   NM_139027
VERSION     NM_139027.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5151..5242 
                     /label="Myc-DDK"  
     misc_feature    5272..5857 
                     /label="PolyA signal"  
     misc_feature    5329..5348 
                     /label="Restriction Enzyme"  
     misc_feature    6133..6805 
                     /label="ColE1"  
     misc_feature    7235..8029 
                     /label="Kan/Neo"  
     misc_feature    8064..8366 
                     /label="SV40 ori"  
     misc_feature    8452..8929 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5133..5139 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5133 
                     /label="ADAMTS13"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCACCAGCGT CACCCCCGGG CAAGATGCCC TCCCCTCTGT GTGGCCGGAA 
     1081 TCCTTGCCTG TGGCTTTCTC CTGGGCTGCT GGGGACCCTC CCATTTCCAG CAGAGTTGTC 
     1141 TTCAGGCTTT GGAGCCACAG GCCGTGTCTT CTTACTTGAG CCCTGGTGCT CCCTTAAAAG 
     1201 GCCGCCCTCC TTCCCCTGGC TTCCAGAGGC AGAGGCAGAG GCAGAGGCGG GCTGCAGGCG 
     1261 GCATCCTACA CCTGGAGCTG CTGGTGGCCG TGGGCCCCGA TGTCTTCCAG GCTCACCAGG 
     1321 AGGACACAGA GCGCTATGTG CTCACCAACC TCAACATCGG GGCAGAACTG CTTCGGGACC 
     1381 CGTCCCTGGG GGCTCAGTTT CGGGTGCACC TGGTGAAGAT GGTCATTCTG ACAGAGCCTG 
     1441 AGGGTGCCCC AAATATCACA GCCAACCTCA CCTCGTCCCT GCTGAGCGTC TGTGGGTGGA 
     1501 GCCAGACCAT CAACCCTGAG GACGACACGG ATCCTGGCCA TGCTGACCTG GTCCTCTATA 
     1561 TCACTAGGTT TGACCTGGAG TTGCCTGATG GTAACCGGCA GGTGCGGGGC GTCACCCAGC 
     1621 TGGGCGGTGC CTGCTCCCCA ACCTGGAGCT GCCTCATTAC CGAGGACACT GGCTTCGACC 
     1681 TGGGAGTCAC CATTGCCCAT GAGATTGGGC ACAGCTTCGG CCTGGAGCAC GACGGCGCGC 
     1741 CCGGCAGCGG CTGCGGCCCC AGCGGACACG TGATGGCTTC GGACGGCGCC GCGCCCCGCG 
     1801 CCGGCCTCGC CTGGTCCCCC TGCAGCCGCC GGCAGCTGCT GAGCCTGCTC AGCGCAGGAC 
     1861 GGGCGCGCTG CGTGTGGGAC CCGCCGCGGC CTCAACCCGG GTCCGCGGGG CACCCGCCGG 
     1921 ATGCGCAGCC TGGCCTCTAC TACAGCGCCA ACGAGCAGTG CCGCGTGGCC TTCGGCCCCA 
     1981 AGGCTGTCGC CTGCACCTTC GCCAGGGAGC ACCTGGATAT GTGCCAGGCC CTCTCCTGCC 
     2041 ACACAGACCC GCTGGACCAA AGCAGCTGCA GCCGCCTCCT CGTTCCTCTC CTGGATGGGA 
     2101 CAGAATGTGG CGTGGAGAAG TGGTGCTCCA AGGGTCGCTG CCGCTCCCTG GTGGAGCTGA 
     2161 CCCCCATAGC AGCAGTGCAT GGGCGCTGGT CTAGCTGGGG TCCCCGAAGT CCTTGCTCCC 
     2221 GCTCCTGCGG AGGAGGTGTG GTCACCAGGA GGCGGCAGTG CAACAACCCC AGACCTGCCT 
     2281 TTGGGGGGCG TGCATGTGTT GGTGCTGACC TCCAGGCCGA GATGTGCAAC ACTCAGGCCT 
     2341 GCGAGAAGAC CCAGCTGGAG TTCATGTCGC AACAGTGCGC CAGGACCGAC GGCCAGCCGC 
     2401 TGCGCTCCTC CCCTGGCGGC GCCTCCTTCT ACCACTGGGG TGCTGCTGTA CCACACAGCC 
     2461 AAGGGGATGC TCTGTGCAGA CACATGTGCC GGGCCATTGG CGAGAGCTTC ATCATGAAGC 
     2521 GTGGAGACAG CTTCCTCGAT GGGACCCGGT GTATGCCAAG TGGCCCCCGG GAGGACGGGA 
     2581 CCCTGAGCCT GTGTGTGTCG GGCAGCTGCA GGACATTTGG CTGTGATGGT AGGATGGACT 
     2641 CCCAGCAGGT ATGGGACAGG TGCCAGGTGT GTGGTGGGGA CAACAGCACG TGCAGCCCAC 
     2701 GGAAGGGCTC TTTCACAGCT GGCAGAGCGA GAGAATATGT CACATTTCTG ACAGTTACCC 
     2761 CCAACCTGAC CAGTGTCTAC ATTGCCAACC ACAGGCCTCT CTTCACACAC TTGGCGGTGA 
     2821 GGATCGGAGG GCGCTATGTC GTGGCTGGGA AGATGAGCAT CTCCCCTAAC ACCACCTACC 
     2881 CCTCCCTCCT GGAGGATGGT CGTGTCGAGT ACAGAGTGGC CCTCACCGAG GACCGGCTGC 
     2941 CCCGCCTGGA GGAGATCCGC ATCTGGGGAC CCCTCCAGGA AGATGCTGAC ATCCAGGTTT 
     3001 ACAGGCGGTA TGGCGAGGAG TATGGCAACC TCACCCGCCC AGACATCACC TTCACCTACT 
     3061 TCCAGCCTAA GCCACGGCAG GCCTGGGTGT GGGCCGCTGT GCGTGGGCCC TGCTCGGTGA 
     3121 GCTGTGGGGC AGGGCTGCGC TGGGTAAACT ACAGCTGCCT GGACCAGGCC AGGAAGGAGT 
     3181 TGGTGGAGAC TGTCCAGTGC CAAGGGAGCC AGCAGCCACC AGCGTGGCCA GAGGCCTGCG 
     3241 TGCTCGAACC CTGCCCTCCC TACTGGGCGG TGGGAGACTT CGGCCCATGC AGCGCCTCCT 
     3301 GTGGGGGCGG CCTGCGGGAG CGGCCAGTGC GCTGCGTGGA GGCCCAGGGC AGCCTCCTGA 
     3361 AGACATTGCC CCCAGCCCGG TGCAGAGCAG GGGCCCAGCA GCCAGCTGTG GCGCTGGAAA 
     3421 CCTGCAACCC CCAGCCCTGC CCTGCCAGGT GGGAGGTGTC AGAGCCCAGC TCATGCACAT 
     3481 CAGCTGGTGG AGCAGGCCTG GCCTTGGAGA ACGAGACCTG TGTGCCAGGG GCAGATGGCC 
     3541 TGGAGGCTCC AGTGACTGAG GGGCCTGGCT CCGTAGATGA GAAGCTGCCT GCCCCTGAGC 
     3601 CCTGTGTCGG GATGTCATGT CCTCCAGGCT GGGGCCATCT GGATGCCACC TCTGCAGGGG 
     3661 AGAAGGCTCC CTCCCCATGG GGCAGCATCA GGACGGGGGC TCAAGCTGCA CACGTGTGGA 
     3721 CCCCTGCGGC AGGGTCGTGC TCCGTCTCCT GCGGGCGAGG TCTGATGGAG CTGCGTTTCC 
     3781 TGTGCATGGA CTCTGCCCTC AGGGTGCCTG TCCAGGAAGA GCTGTGTGGC CTGGCAAGCA 
     3841 AGCCTGGGAG CCGGCGGGAG GTCTGCCAGG CTGTCCCGTG CCCTGCTCGG TGGCAGTACA 
     3901 AGCTGGCGGC CTGCAGCGTG AGCTGTGGGA GAGGGGTCGT GCGGAGGATC CTGTATTGTG 
     3961 CCCGGGCCCA TGGGGAGGAC GATGGTGAGG AGATCCTGTT GGACACCCAG TGCCAGGGGC 
     4021 TGCCTCGCCC GGAACCCCAG GAGGCCTGCA GCCTGGAGCC CTGCCCACCT AGGTGGAAAG 
     4081 TCATGTCCCT TGGCCCATGT TCGGCCAGCT GTGGCCTTGG CACTGCTAGA CGCTCGGTGG 
     4141 CCTGTGTGCA GCTCGACCAA GGCCAGGACG TGGAGGTGGA CGAGGCGGCC TGTGCGGCGC 
     4201 TGGTGCGGCC CGAGGCCAGT GTCCCCTGTC TCATTGCCGA CTGCACCTAC CGCTGGCATG 
     4261 TTGGCACCTG GATGGAGTGC TCTGTTTCCT GTGGGGATGG CATCCAGCGC CGGCGTGACA 
     4321 CCTGCCTCGG ACCCCAGGCC CAGGCGCCTG TGCCAGCTGA TTTCTGCCAG CACTTGCCCA 
     4381 AGCCGGTGAC TGTGCGTGGC TGCTGGGCTG GGCCCTGTGT GGGACAGGGT GCCTGTGGCA 
     4441 GGCAGCACCT TGAGCCAACA GGAACCATTG ACATGCGAGG CCCAGGGCAG GCAGACTGTG 
     4501 CAGTGGCCAT TGGGCGGCCC CTCGGGGAGG TGGTGACCCT CCGCGTCCTT GAGAGTTCTC 
     4561 TCAACTGCAG TGCGGGGGAC ATGTTGCTGC TTTGGGGCCG GCTCACCTGG AGGAAGATGT 
     4621 GCAGGAAGCT GTTGGACATG ACTTTCAGCT CCAAGACCAA CACGCTGGTG GTGAGGCAGC 
     4681 GCTGCGGGCG GCCAGGAGGT GGGGTGCTGC TGCGGTATGG GAGCCAGCTT GCTCCTGAAA 
     4741 CCTTCTACAG AGAATGTGAC ATGCAGCTCT TTGGGCCCTG GGGTGAAATC GTGAGCCCCT 
     4801 CGCTGAGTCC AGCCACGAGT AATGCAGGGG GCTGCCGGCT CTTCATTAAT GTGGCTCCGC 
     4861 ACGCACGGAT TGCCATCCAT GCCCTGGCCA CCAACATGGG CGCTGGGACC GAGGGAGCCA 
     4921 ATGCCAGCTA CATCTTGATC CGGGACACCC ACAGCTTGAG GACCACAGCG TTCCATGGGC 
     4981 AGCAGGTGCT CTACTGGGAG TCAGAGAGCA GCCAGGCTGA GATGGAGTTC AGCGAGGGCT 
     5041 TCCTGAAGGC TCAGGCCAGC CTGCGGGGCC AGTACTGGAC CCTCCAATCA TGGGTACCGG 
     5101 AGATGCAGGA CCCTCAGTCC TGGAAGGGAA AGGAAGGAAC CACGCGTACG CGGCCGCTCG 
     5161 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5221 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5281 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5341 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5401 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5461 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5521 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5581 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5641 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5701 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5761 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5821 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5881 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5941 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6001 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6061 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6121 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6181 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6241 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6301 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6361 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6421 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6481 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6541 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6601 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6661 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6721 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6781 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6841 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6901 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6961 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7021 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7081 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7141 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7201 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7261 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7321 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7381 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7441 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7501 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7561 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7621 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7681 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7741 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7801 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7861 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7921 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7981 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8041 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8101 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8161 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8221 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8281 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8341 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8401 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8461 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8521 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8581 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8641 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8701 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8761 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8821 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8881 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8941 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//