LOCUS       RC217352                7971 bp    DNA     circular  SYN 
DEFINITION  SEMA6A (Myc-DDK-tagged)-Human sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A (SEMA6A)
ACCESSION   NM_020796
VERSION     NM_020796.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4128..4219 
                     /label="Myc-DDK"  
     misc_feature    4249..4834 
                     /label="PolyA signal"  
     misc_feature    4306..4325 
                     /label="Restriction Enzyme"  
     misc_feature    5110..5782 
                     /label="ColE1"  
     misc_feature    6212..7006 
                     /label="Kan/Neo"  
     misc_feature    7041..7343 
                     /label="SV40 ori"  
     misc_feature    7429..7906 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4110..4116 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4110 
                     /label="SEMA6A"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGTCAGAA GCCTTGCTGC TATATTTCAC ACTGCTACAC TTTGCTGGGG 
     1081 CTGGTTTCCC AGAAGATTCT GAGCCAATCA GTATTTCGCA TGGCAACTAT ACAAAACAGT 
     1141 ATCCGGTGTT TGTGGGCCAC AAGCCAGGAC GGAACACCAC ACAGAGGCAC AGGCTGGACA 
     1201 TCCAGATGAT TATGATCATG AACGGAACCC TCTACATTGC TGCTAGGGAC CATATTTATA 
     1261 CTGTTGATAT AGACACATCA CACACGGAAG AAATTTATTG TAGCAAAAAA CTGACATGGA 
     1321 AATCTAGACA GGCCGATGTA GACACATGCA GAATGAAGGG AAAACATAAG GATGAGTGCC 
     1381 ACAACTTTAT TAAAGTTCTT CTAAAGAAAA ACGATGATGC ATTGTTTGTC TGTGGAACTA 
     1441 ATGCCTTCAA CCCTTCCTGC AGAAACTATA AGATGGATAC ATTGGAACCA TTCGGGGATG 
     1501 AATTCAGCGG AATGGCCAGA TGCCCATATG ATGCCAAACA TGCCAACGTT GCACTGTTTG 
     1561 CAGATGGAAA ACTATACTCA GCCACAGTGA CTGACTTCCT TGCCATTGAC GCAGTCATTT 
     1621 ACCGGAGTCT TGGAGAAAGC CCTACCCTGC GGACCGTCAA GCACGATTCA AAATGGTTGA 
     1681 AAGAACCATA CTTTGTTCAA GCCGTGGATT ACGGAGATTA TATCTACTTC TTCTTCAGGG 
     1741 AAATAGCAGT GGAGTATAAC ACCATGGGAA AGGTAGTTTT CCCAAGAGTG GCTCAGGTTT 
     1801 GTAAGAATGA TATGGGAGGA TCTCAAAGAG TCCTGGAGAA ACAGTGGACG TCGTTCCTGA 
     1861 AGGCGCGCTT GAACTGCTCA GTTCCTGGAG ACTCTCATTT TTATTTCAAC ATTCTCCAGG 
     1921 CAGTTACAGA TGTGATTCGT ATCAACGGGC GTGATGTTGT CCTGGCAACG TTTTCTACAC 
     1981 CTTATAACAG CATCCCTGGG TCTGCAGTCT GTGCCTATGA CATGCTTGAC ATTGCCAGTG 
     2041 TTTTTACTGG GAGATTCAAG GAACAGAAGT CTCCTGATTC CACCTGGACA CCAGTTCCTG 
     2101 ATGAACGAGT TCCTAAGCCC AGGCCAGGTT GCTGTGCTGG CTCATCCTCC TTAGAAAGAT 
     2161 ATGCAACCTC CAATGAGTTC CCTGATGATA CCCTGAACTT CATCAAGACG CACCCGCTCA 
     2221 TGGATGAGGC AGTGCCCTCC ATCTTCAACA GGCCATGGTT CCTGAGAACA ATGGTCAGAT 
     2281 ACCGCCTTAC CAAAATTGCA GTGGACACAG CTGCTGGGCC ATATCAGAAT CACACTGTGG 
     2341 TTTTTCTGGG ATCAGAGAAG GGAATCATCT TGAAGTTTTT GGCCAGAATA GGAAATAGTG 
     2401 GTTTTCTAAA TGACAGCCTT TTCCTGGAGG AGATGAGTGT TTACAACTCT GAAAAATGCA 
     2461 GCTATGATGG AGTCGAAGAC AAAAGGATCA TGGGCATGCA GCTGGACAGA GCAAGCAGCT 
     2521 CTCTGTATGT TGCGTTCTCT ACCTGTGTGA TAAAGGTTCC CCTTGGCCGG TGTGAACGAC 
     2581 ATGGGAAGTG TAAAAAAACC TGTATTGCCT CCAGAGACCC ATATTGTGGA TGGATAAAGG 
     2641 AAGGTGGTGC CTGCAGCCAT TTATCACCCA ACAGCAGACT GACTTTTGAG CAGGACATAG 
     2701 AGCGTGGCAA TACAGATGGT CTGGGGGACT GTCACAATTC CTTTGTGGCA CTGAATGGGC 
     2761 ATTCCAGTTC CCTCTTGCCC AGCACAACCA CATCAGATTC GACGGCTCAA GAGGGGTATG 
     2821 AGTCTAGGGG AGGAATGCTG GACTGGAAGC ATCTGCTTGA CTCACCTGAC AGCACAGACC 
     2881 CTTTGGGGGC AGTGTCTTCC CATAATCACC AAGACAAGAA GGGAGTGATT CGGGAAAGTT 
     2941 ACCTCAAAGG CCACGACCAG CTGGTTCCCG TCACCCTCTT GGCCATTGCA GTCATCCTGG 
     3001 CTTTCGTCAT GGGGGCCGTC TTCTCGGGCA TCACCGTCTA CTGCGTCTGT GATCATCGGC 
     3061 GCAAAGACGT GGCTGTGGTG CAGCGCAAGG AGAAGGAGCT CACCCACTCG CGCCGGGGCT 
     3121 CCATGAGCAG CGTCACCAAG CTCAGCGGCC TCTTTGGGGA CACTCAATCC AAAGACCCAA 
     3181 AGCCGGAGGC CATCCTCACG CCACTCATGC ACAACGGCAA GCTCGCCACT CCCGGCAACA 
     3241 CGGCCAAGAT GCTCATTAAA GCAGACCAGC ACCACCTGGA CCTGACGGCC CTCCCCACCC 
     3301 CAGAGTCAAC CCCAACGCTG CAGCAGAAGC GGAAGCCCAG CCGCGGCAGC CGCGAGTGGG 
     3361 AGAGGAACCA GAACCTCATC AATGCCTGCA CAAAGGACAT GCCCCCCATG GGCTCCCCTG 
     3421 TGATTCCCAC GGACCTGCCC CTGCGGGCCT CCCCCAGCCA CATCCCCAGC GTGGTGGTCC 
     3481 TGCCCATCAC GCAGCAGGGC TACCAGCATG AGTACGTGGA CCAGCCCAAA ATGAGCGAGG 
     3541 TGGCCCAGAT GGCGCTGGAG GACCAGGCCG CCACACTGGA GTATAAGACC ATCAAGGAAC 
     3601 ATCTCAGCAG CAAGAGTCCC AACCATGGGG TGAACCTTGT GGAGAACCTG GACAGCCTGC 
     3661 CCCCCAAAGT TCCACAGCGG GAGGCCTCCC TGGGTCCCCC GGGAGCCTCC CTGTCTCAGA 
     3721 CCGGTCTAAG CAAGCGGCTG GAAATGCACC ACTCCTCTTC CTACGGGGTT GACTATAAGA 
     3781 GGAGCTACCC CACGAACTCG CTCACGAGAA GCCACCAGGC CACCACTCTC AAAAGAAACA 
     3841 ACACTAACTC CTCCAATTCC TCTCACCTCT CCAGAAACCA GAGCTTTGGC AGGGGAGACA 
     3901 ACCCGCCGCC CGCCCCGCAG AGGGTGGACT CCATCCAGGT GCACAGCTCC CAGCCATCTG 
     3961 GCCAGGCCGT GACTGTCTCG AGGCAGCCCA GCCTCAACGC CTACAACTCA CTGACAAGGT 
     4021 CGGGGCTGAA GCGTACGCCC TCGCTAAAGC CGGACGTACC CCCCAAACCA TCCTTTGCTC 
     4081 CCCTTTCCAC ATCCATGAAG CCCAATGATG CGTGTACAAC GCGTACGCGG CCGCTCGAGC 
     4141 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4201 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4261 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4321 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4381 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4441 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4501 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4561 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4621 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4681 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4741 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4801 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4861 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     4921 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     4981 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5041 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5101 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5161 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5221 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5281 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5341 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5401 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5461 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5521 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5581 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5641 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5701 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5761 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5821 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     5881 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     5941 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6001 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6061 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6121 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6181 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6241 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6301 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6361 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6421 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6481 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6541 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6601 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6661 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6721 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6781 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6841 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     6901 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     6961 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7021 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7081 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7141 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7201 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7261 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7321 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7381 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7441 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7501 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7561 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7621 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7681 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7741 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7801 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7861 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     7921 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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