LOCUS       RC217317                8817 bp    DNA     circular  SYN 
DEFINITION  MINK1 (Myc-DDK-tagged)-Human misshapen-like kinase 1 (MINK1), transcript variant 4
ACCESSION   NM_001024937
VERSION     NM_001024937.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4974..5065 
                     /label="Myc-DDK"  
     misc_feature    5095..5680 
                     /label="PolyA signal"  
     misc_feature    5152..5171 
                     /label="Restriction Enzyme"  
     misc_feature    5956..6628 
                     /label="ColE1"  
     misc_feature    7058..7852 
                     /label="Kan/Neo"  
     misc_feature    7887..8189 
                     /label="SV40 ori"  
     misc_feature    8275..8752 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4956..4962 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4956 
                     /label="MINK1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCGACCCA GCCCCCGCCC GCAGCCTGGA CGACATCGAC CTGTCCGCCC 
     1081 TGCGGGACCC TGCTGGGATC TTTGAGCTTG TGGAGGTGGT CGGCAATGGA ACCTACGGAC 
     1141 AGGTGTACAA GGGTCGGCAT GTCAAGACGG GGCAGCTGGC TGCCATCAAG GTCATGGATG 
     1201 TCACGGAGGA CGAGGAGGAA GAGATCAAAC AGGAGATCAA CATGCTGAAA AAGTACTCTC 
     1261 ACCACCGCAA CATCGCCACC TACTACGGAG CCTTCATCAA GAAGAGCCCC CCGGGAAACG 
     1321 ATGACCAGCT CTGGCTGGTG ATGGAGTTCT GTGGTGCTGG TTCAGTGACT GACCTGGTAA 
     1381 AGAACACAAA AGGCAACGCC CTGAAGGAGG ACTGTATCGC CTATATCTGC AGGGAGATCC 
     1441 TCAGGGGTCT GGCCCATCTC CATGCCCACA AGGTGATCCA TCGAGACATC AAGGGGCAGA 
     1501 ATGTGCTGCT GACAGAGAAT GCTGAGGTCA AGCTAGTGGA TTTTGGGGTG AGTGCTCAGC 
     1561 TGGACCGCAC CGTGGGCAGA CGGAACACTT TCATTGGGAC TCCCTACTGG ATGGCTCCAG 
     1621 AGGTCATCGC CTGTGATGAG AACCCTGATG CCACCTATGA TTACAGGAGT GATATTTGGT 
     1681 CTCTAGGAAT CACAGCCATC GAGATGGCAG AGGGAGCCCC CCCTCTGTGT GACATGCACC 
     1741 CCATGCGAGC CCTCTTCCTC ATTCCTCGGA ACCCTCCGCC CAGGCTCAAG TCCAAGAAGT 
     1801 GGTCTAAGAA GTTCATTGAC TTCATTGACA CATGTCTCAT CAAGACTTAC CTGAGCCGCC 
     1861 CACCCACGGA GCAGCTACTG AAGTTTCCCT TCATCCGGGA CCAGCCCACG GAGCGGCAGG 
     1921 TCCGCATCCA GCTTAAGGAC CACATTGACC GATCCCGGAA GAAGCGGGGT GAGAAAGAGG 
     1981 AGACAGAATA TGAGTACAGC GGCAGCGAGG AGGAAGATGA CAGCCATGGA GAGGAAGGAG 
     2041 AGCCAAGCTC CATCATGAAC GTGCCTGGAG AGTCGACTCT ACGCCGGGAG TTTCTCCGGC 
     2101 TCCAGCAGGA AAATAAGAGC AACTCAGAGG CTTTAAAACA GCAGCAGCAG CTGCAGCAGC 
     2161 AGCAGCAGCG AGACCCCGAG GCACACATCA AACACCTGCT GCACCAGCGG CAGCGGCGCA 
     2221 TAGAGGAGCA GAAGGAGGAG CGGCGCCGCG TGGAGGAGCA ACAGCGGCGG GAGCGGGAGC 
     2281 AGCGGAAGCT GCAGGAGAAG GAGCAGCAGC GGCGGCTGGA GGACATGCAG GCTCTGCGGC 
     2341 GGGAGGAGGA GCGGCGGCAG GCGGAGCGCG AGCAGGAATA CAAGCGGAAG CAGCTGGAGG 
     2401 AGCAGCGGCA GTCAGAACGT CTCCAGAGGC AGCTGCAGCA GGAGCATGCC TACCTCAAGT 
     2461 CCCTGCAGCA GCAGCAACAG CAGCAGCAGC TTCAGAAACA GCAGCAGCAG CAGCTCCTGC 
     2521 CTGGGGACAG GAAGCCCCTG TACCATTATG GTCGGGGCAT GAATCCCGCT GACAAACCAG 
     2581 CCTGGGCCCG AGAGGTAGAA GAGAGAACAA GGATGAACAA GCAGCAGAAC TCTCCCTTGG 
     2641 CCAAGAGCAA GCCAGGCAGC ACGGGGCCTG AGCCCCCCAT CCCCCAGGCC TCCCCAGGGC 
     2701 CCCCAGGACC CCTTTCCCAG ACTCCTCCTA TGCAGAGGCC GGTGGAGCCC CAGGAGGGAC 
     2761 CGCACAAGTC CCTGCAGGAC CAGCCCACCC GAAACCTGGC TGCCTTCCCA GCCTCCCATG 
     2821 ACCCCGACCC TGCCATCCCC GCACCCACTG CCACGCCCAG TGCCCGAGGA GCTGTCATCC 
     2881 GCCAGAATTC AGACCCCACC TCTGAAGGAC CTGGCCCCAG CCCGAATCCC CCAGCCTGGG 
     2941 TCCGCCCAGA TAACGAGGCC CCACCCAAGG TGCCTCAGAG GACCTCATCT ATCGCCACTG 
     3001 CCCTTAACAC CAGTGGGGCC GGAGGGTCCC GGCCAGCCCA GGCAGTCCGT GCCAGACCTC 
     3061 GCAGCAACTC CGCCTGGCAA ATCTATCTGC AAAGGCGGGC AGAGCGGGGC ACCCCAAAGC 
     3121 CTCCAGGGCC CCCTGCTCAG CCCCCTGGCC CGCCCAACGC CTCTAGTAAC CCCGACCTCA 
     3181 GGAGGAGCGA CCCTGGCTGG GAACGCTCGG ACAGCGTCCT TCCAGCCTCT CACGGGCACC 
     3241 TCCCCCAGGC TGGCTCACTG GAGCGGAACC GCGTGGGAGT CTCCTCCAAA CCGGACAGCT 
     3301 CCCCTGTGCT CTCCCCTGGG AATAAAGCCA AGCCCGACGA CCACCGCTCA CGGCCAGGCC 
     3361 GGCCCGCAGA CTTTGTGTTG CTGAAAGAGC GGACTCTGGA CGAGGCCCCT CGGCCTCCCA 
     3421 AGAAGGCCAT GGACTACTCG TCGTCCAGCG AGGAGGTGGA AAGCAGTGAG GACGACGAGG 
     3481 AGGAAGGCGA AGGCGGGCCA GCAGAGGGGA GCAGAGATAC CCCTGGGGGC CGCAGCGATG 
     3541 GGGATACAGA CAGCGTCAGC ACCATGGTGG TCCACGACGT CGAGGAGATC ACCGGGACCC 
     3601 AGCCCCCATA CGGGGGCGGC ACCATGGTGG TCCAGCGCAC CCCTGAAGAG GAGCGGAACC 
     3661 TGCTGCATGC TGACAGCAAT GGGTACACAA ACCTGCCTGA CGTGGTCCAG CCCAGCCACT 
     3721 CACCCACCGA GAACAGCAAA GGCCAAAGCC CACCCTCGAA GGATGGGAGT GGTGACTACC 
     3781 AGTCTCGTGG GCTGGTAAAG GCCCCTGGCA AGAGCTCGTT CACGATGTTT GTGGATCTAG 
     3841 GGATCTACCA GCCTGGAGGC AGTGGGGACA GCATCCCCAT CACAGCCCTA GTGGGTGGAG 
     3901 AGGGCACTCG GCTCGACCAG CTGCAGTACG ACGTGAGGAA GGGTTCTGTG GTCAACGTGA 
     3961 ATCCCACCAA CACCCGGGCC CACAGTGAGA CCCCTGAGAT CCGGAAGTAC AAGAAGCGAT 
     4021 TCAACTCCGA GATCCTCTGT GCAGCCCTTT GGGGGGTCAA CCTGCTGGTG GGCACGGAGA 
     4081 ACGGGCTGAT GTTGCTGGAC CGAAGTGGGC AGGGCAAGGT GTATGGACTC ATTGGGCGGC 
     4141 GACGCTTCCA GCAGATGGAT GTGCTGGAGG GGCTCAACCT GCTCATCACC ATCTCAGGGA 
     4201 AAAGGAACAA ACTGCGGGTG TATTACCTGT CCTGGCTCCG GAACAAGATT CTGCACAATG 
     4261 ACCCAGAAGT GGAGAAGAAG CAGGGCTGGA CCACCGTGGG GGACATGGAG GGCTGCGGGC 
     4321 ACTACCGTGT TGTGAAATAC GAGCGGATTA AGTTCCTGGT CATCGCCCTC AAGAGCTCCG 
     4381 TGGAGGTGTA TGCCTGGGCC CCCAAACCCT ACCACAAATT CATGGCCTTC AAGTCCTTTG 
     4441 CCGACCTCCC CCACCGCCCT CTGCTGGTCG ACCTGACAGT AGAGGAGGGG CAGCGGCTCA 
     4501 AGGTCATCTA TGGCTCCAGT GCTGGCTTCC ATGCTGTGGA TGTCGACTCG GGGAACAGCT 
     4561 ATGACATCTA CATCCCTGTG CACATCCAGA GCCAGATCAC GCCCCATGCC ATCATCTTCC 
     4621 TCCCCAACAC CGACGGCATG GAGATGCTGC TGTGCTACGA GGACGAGGGT GTCTACGTCA 
     4681 ACACGTACGG GCGCATCATT AAGGATGTGG TGCTGCAGTG GGGGGAGATG CCTACTTCTG 
     4741 TGGCCTACAT CTGCTCCAAC CAGATAATGG GCTGGGGTGA GAAAGCCATT GAGATCCGCT 
     4801 CTGTGGAGAC GGGCCACCTC GACGGGGTCT TCATGCACAA ACGAGCTCAG AGGCTCAAGT 
     4861 TCCTGTGTGA GCGGAATGAC AAGGTGTTTT TTGCCTCAGT CCGCTCTGGG GGCAGCAGCC 
     4921 AAGTTTACTT CATGACTCTG AACCGTAACT GCATCATGAA CTGGACGCGT ACGCGGCCGC 
     4981 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     5041 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     5101 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5161 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5221 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5281 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5341 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5401 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5461 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5521 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5581 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5641 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5701 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5761 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5821 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5881 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5941 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     6001 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     6061 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6121 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6181 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6241 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6301 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6361 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6421 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6481 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6541 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6601 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6661 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6721 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6781 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6841 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6901 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6961 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     7021 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     7081 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7141 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7201 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7261 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7321 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7381 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7441 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7501 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7561 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7621 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7681 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7741 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7801 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7861 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7921 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7981 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     8041 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8221 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8281 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8341 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8401 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8461 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8521 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8581 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8641 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8701 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8761 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//